Mutagenetix > Incidental Mutation

Incidental Mutation 'R4601:Itgb4'

345649

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4601 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

115865556-115899238 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 115896548 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1436 (T1436A)

Ref Sequence

ENSEMBL: ENSMUSP00000070811 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000021114] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

possibly damaging
Transcript: ENSMUST00000021107
AA Change: T1432A

PolyPhen 2 Score 0.752 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: T1432A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000021114

SMART Domains

Protein: ENSMUSP00000021114
Gene: ENSMUSG00000020766

Domain	Start	End	E-Value	Type
Pfam:GalKase_gal_bdg	18	67	2.6e-24	PFAM
Pfam:GHMP_kinases_N	126	194	7.3e-14	PFAM
Pfam:GHMP_kinases_C	289	374	1.6e-14	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000068981
AA Change: T1436A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: T1436A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106458
AA Change: T1501A

PolyPhen 2 Score 0.055 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: T1501A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106460
AA Change: T1436A

PolyPhen 2 Score 0.128 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: T1436A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106461
AA Change: T1501A

PolyPhen 2 Score 0.055 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: T1501A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000169928
AA Change: T1432A

PolyPhen 2 Score 0.752 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: T1432A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 94.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	T	A	17: 45,827,847 (GRCm39)	D555E	probably damaging	Het
Abca16	T	G	7: 120,035,920 (GRCm39)	F334L	probably damaging	Het
Abcc4	T	A	14: 118,869,575 (GRCm39)	M186L	probably benign	Het
Agap3	T	C	5: 24,681,406 (GRCm39)	L120P	probably damaging	Het
Ak7	G	A	12: 105,679,834 (GRCm39)	V123M	probably benign	Het
Aldh1a7	A	T	19: 20,693,343 (GRCm39)	V192D	probably damaging	Het
Ankrd36	A	G	11: 5,520,102 (GRCm39)	D59G	probably benign	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Cacna1e	A	G	1: 154,347,359 (GRCm39)	V936A	probably benign	Het
Camkv	T	C	9: 107,823,295 (GRCm39)	V107A	probably damaging	Het
Camp	T	C	9: 109,677,730 (GRCm39)	E80G	probably damaging	Het
Cblif	A	C	19: 11,729,554 (GRCm39)	D171A	probably damaging	Het
Ccdc66	T	C	14: 27,222,377 (GRCm39)	N122S	probably damaging	Het
Cd101	G	A	3: 100,901,204 (GRCm39)	T960M	possibly damaging	Het
Cdk18	A	G	1: 132,044,657 (GRCm39)	V323A	possibly damaging	Het
Celf2	G	T	2: 6,590,831 (GRCm39)	N279K	possibly damaging	Het
Cemip	T	C	7: 83,600,826 (GRCm39)	I932V	probably damaging	Het
Cep250	C	A	2: 155,803,973 (GRCm39)	Q28K	probably benign	Het
Ces2f	T	C	8: 105,676,596 (GRCm39)	C97R	probably damaging	Het
Cfap126	G	T	1: 170,941,627 (GRCm39)	G41C	possibly damaging	Het
Cfap96	A	T	8: 46,423,505 (GRCm39)	I69N	probably damaging	Het
Chat	T	C	14: 32,146,112 (GRCm39)	M354V	probably benign	Het
Clca4b	A	T	3: 144,632,945 (GRCm39)	D168E	possibly damaging	Het
Cpxm1	T	A	2: 130,235,496 (GRCm39)	M499L	possibly damaging	Het
Dennd3	T	A	15: 73,439,009 (GRCm39)	W1126R	probably damaging	Het
Dgkb	T	C	12: 38,652,819 (GRCm39)	S735P	probably damaging	Het
Dlec1	T	C	9: 118,976,202 (GRCm39)		probably null	Het
Dnajc6	T	C	4: 101,468,461 (GRCm39)	F166L	probably damaging	Het
Dph5	A	G	3: 115,693,426 (GRCm39)	N115D	possibly damaging	Het
Ercc3	G	A	18: 32,378,624 (GRCm39)	A202T	probably benign	Het
Erich3	A	T	3: 154,470,375 (GRCm39)	D136V	unknown	Het
Exoc2	T	C	13: 31,066,251 (GRCm39)	N475S	probably benign	Het
Fam217a	T	C	13: 35,095,285 (GRCm39)	D310G	probably damaging	Het
Fam53a	C	T	5: 33,758,007 (GRCm39)	S372N	probably benign	Het
Fbn2	C	G	18: 58,186,805 (GRCm39)	G1699R	probably damaging	Het
Fbxo46	T	C	7: 18,869,489 (GRCm39)	V36A	probably benign	Het
Fhip1a	T	C	3: 85,648,487 (GRCm39)	M26V	probably damaging	Het
G6pc1	A	G	11: 101,263,567 (GRCm39)	Y127C	probably damaging	Het
Gba2	A	G	4: 43,573,810 (GRCm39)	F161L	probably damaging	Het
Gja1	T	C	10: 56,264,325 (GRCm39)	L228P	probably damaging	Het
Gm20834	A	G	Y: 10,323,178 (GRCm39)	V86A	probably benign	Het
Hmcn1	A	T	1: 150,614,396 (GRCm39)	C1337S	probably damaging	Het
Il1rl1	T	A	1: 40,480,460 (GRCm39)	S30T	possibly damaging	Het
Itk	T	A	11: 46,227,342 (GRCm39)	Q427L	probably benign	Het
Klhdc4	T	C	8: 122,526,266 (GRCm39)	E291G	probably damaging	Het
Map4	T	A	9: 109,881,887 (GRCm39)	S250R	possibly damaging	Het
Mnt	T	A	11: 74,727,285 (GRCm39)	V57E	possibly damaging	Het
Mrc2	G	A	11: 105,239,257 (GRCm39)		probably null	Het
Mslnl	G	A	17: 25,961,908 (GRCm39)	V128M	probably damaging	Het
Mtcl2	T	C	2: 156,881,844 (GRCm39)	K736R	probably benign	Het
Musk	A	T	4: 58,301,625 (GRCm39)	I128F	probably damaging	Het
Myh4	G	A	11: 67,141,136 (GRCm39)	A733T	possibly damaging	Het
Myo5a	T	A	9: 75,043,670 (GRCm39)	F220I	probably damaging	Het
Npas3	A	G	12: 54,091,361 (GRCm39)	H305R	probably damaging	Het
Nrcam	A	T	12: 44,637,839 (GRCm39)	Y1132F	probably damaging	Het
Nt5c3b	A	C	11: 100,323,744 (GRCm39)	D189E	probably benign	Het
Nuf2	A	G	1: 169,333,683 (GRCm39)	L331P	probably damaging	Het
Nup214	C	T	2: 31,887,977 (GRCm39)	T646I	probably benign	Het
Or14j9	T	C	17: 37,875,076 (GRCm39)	N42S	probably damaging	Het
Pcdh18	T	C	3: 49,699,174 (GRCm39)	E1096G	probably damaging	Het
Pgm2	T	A	5: 64,265,070 (GRCm39)	F364I	probably benign	Het
Pikfyve	A	G	1: 65,273,421 (GRCm39)	N612S	probably damaging	Het
Pla2g4e	T	G	2: 120,016,863 (GRCm39)	H226P	possibly damaging	Het
Ppp3cb	T	C	14: 20,570,714 (GRCm39)	N339S	possibly damaging	Het
Prkd2	G	T	7: 16,577,573 (GRCm39)		probably benign	Het
Rnf133	T	C	6: 23,649,041 (GRCm39)	E296G	possibly damaging	Het
Sav1	A	T	12: 70,031,095 (GRCm39)	D142E	probably benign	Het
Scn3b	C	A	9: 40,199,719 (GRCm39)	P212T	probably damaging	Het
Sel1l	A	T	12: 91,799,827 (GRCm39)		probably null	Het
Septin9	A	G	11: 117,251,310 (GRCm39)	K543E	probably damaging	Het
Serpinb12	T	A	1: 106,876,883 (GRCm39)	D66E	probably benign	Het
Slc35e2	T	C	4: 155,702,106 (GRCm39)	F290S	probably benign	Het
Snrpa	A	G	7: 26,894,958 (GRCm39)	M1T	probably null	Het
Sptlc3	G	A	2: 139,478,600 (GRCm39)	V520I	probably benign	Het
Srpk3	A	G	X: 72,818,547 (GRCm39)	H79R	possibly damaging	Het
Stox2	A	G	8: 47,645,970 (GRCm39)	S497P	probably damaging	Het
Stradb	G	A	1: 59,032,731 (GRCm39)	S361N	probably damaging	Het
Sult3a2	T	C	10: 33,658,083 (GRCm39)	K10R	probably benign	Het
Susd4	A	G	1: 182,686,025 (GRCm39)	N192D	probably damaging	Het
Tcp10a	T	A	17: 7,593,374 (GRCm39)	D32E	probably benign	Het
Tdpoz4	T	A	3: 93,704,339 (GRCm39)	V212D	probably damaging	Het
Tdrd5	T	A	1: 156,111,944 (GRCm39)	T479S	probably benign	Het
Tex10	T	C	4: 48,452,946 (GRCm39)	D671G	probably benign	Het
Tex55	A	G	16: 38,648,380 (GRCm39)	V243A	probably benign	Het
Traj37	T	C	14: 54,418,996 (GRCm39)		probably benign	Het
Ttc7b	G	A	12: 100,466,376 (GRCm39)	R79C	probably damaging	Het
Vmn2r115	T	A	17: 23,565,373 (GRCm39)	L420Q	probably benign	Het
Wdr24	T	A	17: 26,047,181 (GRCm39)		probably null	Het
Wdr81	T	A	11: 75,336,484 (GRCm39)	Q516L	probably damaging	Het
Zfp516	A	G	18: 82,974,164 (GRCm39)	T121A	probably benign	Het
Zmat1	A	T	X: 133,873,694 (GRCm39)	S566T	probably damaging	Homo

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,881,766 (GRCm39)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,881,746 (GRCm39)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	115,897,283 (GRCm39)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	115,894,213 (GRCm39)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	115,898,795 (GRCm39)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	115,896,792 (GRCm39)	missense	probably benign
IGL02879:Itgb4	APN	11	115,885,178 (GRCm39)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,879,731 (GRCm39)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,879,550 (GRCm39)	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	115,891,166 (GRCm39)	nonsense	probably null
R0021:Itgb4	UTSW	11	115,870,453 (GRCm39)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,869,950 (GRCm39)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,870,238 (GRCm39)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	115,898,428 (GRCm39)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,870,582 (GRCm39)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,870,521 (GRCm39)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,881,835 (GRCm39)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,870,594 (GRCm39)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,884,168 (GRCm39)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,888,988 (GRCm39)	intron	probably benign
R1381:Itgb4	UTSW	11	115,885,163 (GRCm39)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,881,710 (GRCm39)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,869,937 (GRCm39)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,874,990 (GRCm39)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,874,873 (GRCm39)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,890,625 (GRCm39)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,871,817 (GRCm39)	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115,882,142 (GRCm39)	nonsense	probably null
R1633:Itgb4	UTSW	11	115,898,586 (GRCm39)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	115,898,183 (GRCm39)	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115,882,156 (GRCm39)	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	115,894,315 (GRCm39)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,879,744 (GRCm39)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,879,346 (GRCm39)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,874,590 (GRCm39)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,871,564 (GRCm39)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,884,279 (GRCm39)	nonsense	probably null
R2102:Itgb4	UTSW	11	115,896,561 (GRCm39)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,870,450 (GRCm39)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,884,261 (GRCm39)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	115,897,389 (GRCm39)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	115,894,496 (GRCm39)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	115,896,752 (GRCm39)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	115,897,296 (GRCm39)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,881,761 (GRCm39)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,879,555 (GRCm39)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,890,640 (GRCm39)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,874,087 (GRCm39)	splice site	silent
R4585:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	115,897,431 (GRCm39)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,879,417 (GRCm39)	intron	probably benign
R5084:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	115,897,365 (GRCm39)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,880,575 (GRCm39)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,875,894 (GRCm39)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,879,258 (GRCm39)	intron	probably benign
R5812:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,870,212 (GRCm39)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,885,102 (GRCm39)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	115,891,237 (GRCm39)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,888,963 (GRCm39)	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115,874,897 (GRCm39)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	115,896,391 (GRCm39)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,888,906 (GRCm39)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	115,897,302 (GRCm39)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,870,557 (GRCm39)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	115,894,536 (GRCm39)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	115,894,510 (GRCm39)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	115,891,087 (GRCm39)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,873,525 (GRCm39)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,884,255 (GRCm39)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,882,544 (GRCm39)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,871,853 (GRCm39)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,875,898 (GRCm39)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,879,295 (GRCm39)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,874,879 (GRCm39)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	115,897,923 (GRCm39)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	115,898,130 (GRCm39)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,870,465 (GRCm39)	missense	probably benign
R9289:Itgb4	UTSW	11	115,885,187 (GRCm39)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,880,625 (GRCm39)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	115,895,855 (GRCm39)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,874,097 (GRCm39)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,885,171 (GRCm39)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,882,485 (GRCm39)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,884,278 (GRCm39)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	115,897,346 (GRCm39)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,888,884 (GRCm39)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,877,637 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGATGGACTTTGCCTATCCAG -3'
(R):5'- TAGAGTCTGGTGAACCCCTG -3'

Sequencing Primer
(F):5'- GAATGACTGCAGCCAATG -3'
(R):5'- CCATCTTCCCAGATAGGAGGG -3'

Posted On

2015-09-25