Mutagenetix > Incidental Mutation

Incidental Mutation 'R4681:Vmn2r15'

350023

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r15

Ensembl Gene

ENSMUSG00000091375

Gene Name

vomeronasal 2, receptor 15

Synonyms

EG211223

MMRRC Submission

042015-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R4681 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109434135-109445422 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109434488 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 739 (I739F)

Ref Sequence

ENSEMBL: ENSMUSP00000128333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167133]

AlphaFold

L7N2A0

Predicted Effect

probably damaging
Transcript: ENSMUST00000167133
AA Change: I739F

PolyPhen 2 Score 0.968 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000128333
Gene: ENSMUSG00000091375
AA Change: I739F

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	75	472	1e-29	PFAM
Pfam:NCD3G	514	568	5.8e-18	PFAM
Pfam:7tm_3	601	836	9.1e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahctf1	G	T	1: 179,580,361 (GRCm39)	T1947K	probably benign	Het
Ak9	A	C	10: 41,303,234 (GRCm39)	K1669T	unknown	Het
Atp4b	T	C	8: 13,439,700 (GRCm39)	E174G	probably benign	Het
Bcl6	A	G	16: 23,787,203 (GRCm39)		probably benign	Het
Brca2	C	G	5: 150,475,863 (GRCm39)		probably null	Het
Btnl4	A	T	17: 34,689,075 (GRCm39)		probably null	Het
C4a	G	T	17: 35,036,075 (GRCm39)		noncoding transcript	Het
Cab39l	A	G	14: 59,737,054 (GRCm39)	D58G	probably benign	Het
Cacna2d3	A	T	14: 29,015,092 (GRCm39)	M100K	probably damaging	Het
Car2	T	A	3: 14,960,624 (GRCm39)	Y127*	probably null	Het
Cdhr1	T	C	14: 36,818,194 (GRCm39)	N86S	probably benign	Het
Celsr3	A	G	9: 108,704,953 (GRCm39)	I479V	possibly damaging	Het
Cfhr1	A	T	1: 139,478,667 (GRCm39)	Y53*	probably null	Het
Cgrrf1	A	G	14: 47,091,283 (GRCm39)	E269G	probably benign	Het
Cimap3	C	A	3: 105,905,701 (GRCm39)	G148C	probably damaging	Het
Clcn7	A	T	17: 25,376,935 (GRCm39)	H636L	probably damaging	Het
Cox10	T	G	11: 63,867,277 (GRCm39)	T240P	possibly damaging	Het
Crybg2	CTTCCAGAGCCATGGACCCATCTTTTCCA	CTTCCA	4: 133,800,029 (GRCm39)		probably null	Het
Dbt	A	G	3: 116,326,963 (GRCm39)	D104G	probably damaging	Het
F730035P03Rik	T	C	7: 99,429,425 (GRCm39)		noncoding transcript	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fam186b	T	C	15: 99,178,771 (GRCm39)	K185R	probably benign	Het
Fat4	T	C	3: 38,941,491 (GRCm39)	L128P	probably damaging	Het
Gbf1	A	T	19: 46,268,989 (GRCm39)	Q1381L	probably benign	Het
Glp2r	C	T	11: 67,621,453 (GRCm39)		probably null	Het
Gm18025	A	G	12: 34,340,884 (GRCm39)	S70P	probably benign	Het
Gpr45	A	G	1: 43,072,068 (GRCm39)	D237G	probably benign	Het
Hectd4	A	T	5: 121,441,678 (GRCm39)	L1213F	possibly damaging	Het
Hydin	T	C	8: 111,233,103 (GRCm39)	V1734A	possibly damaging	Het
Kcnh5	A	T	12: 75,054,397 (GRCm39)	S516T	probably benign	Het
Liph	A	C	16: 21,802,777 (GRCm39)	S97R	probably benign	Het
Mtcl1	T	C	17: 66,756,139 (GRCm39)	T68A	unknown	Het
Nsun7	T	A	5: 66,418,542 (GRCm39)	S91T	probably benign	Het
Or5an10	T	C	19: 12,276,413 (GRCm39)	T28A	probably benign	Het
Or6c75	A	G	10: 129,337,433 (GRCm39)	I227V	probably damaging	Het
Pcdh20	A	T	14: 88,705,052 (GRCm39)	N749K	probably damaging	Het
Pot1b	A	T	17: 55,961,831 (GRCm39)	D582E	probably benign	Het
Pxdn	T	C	12: 30,062,325 (GRCm39)	I1212T	probably benign	Het
Ramp1	T	C	1: 91,124,511 (GRCm39)	V24A	probably benign	Het
S100a8	T	A	3: 90,576,890 (GRCm39)	D14E	probably benign	Het
Stk31	A	G	6: 49,414,369 (GRCm39)	D501G	probably benign	Het
Tbc1d19	G	A	5: 54,029,595 (GRCm39)	V319M	probably damaging	Het
Tbcel	G	T	9: 42,361,268 (GRCm39)	H93Q	probably damaging	Het
Traf3ip2	C	T	10: 39,515,256 (GRCm39)	P345S	possibly damaging	Het
Trpm8	A	T	1: 88,312,427 (GRCm39)	I1103F	possibly damaging	Het
Ttc19	T	A	11: 62,199,917 (GRCm39)	C112*	probably null	Het
Unc5c	T	G	3: 141,474,374 (GRCm39)		probably null	Het
Urb1	A	T	16: 90,601,425 (GRCm39)	H115Q	probably damaging	Het
Zcchc14	G	T	8: 122,335,339 (GRCm39)		probably benign	Het
Zfp408	G	A	2: 91,476,131 (GRCm39)	P341L	probably damaging	Het
Zfp638	G	A	6: 83,958,719 (GRCm39)	V1166M	possibly damaging	Het

Other mutations in Vmn2r15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01335:Vmn2r15	APN	5	109,434,602 (GRCm39)	missense	possibly damaging	0.70
IGL01367:Vmn2r15	APN	5	109,441,075 (GRCm39)	missense	probably damaging	0.99
IGL01844:Vmn2r15	APN	5	109,434,135 (GRCm39)	makesense	probably null
IGL02190:Vmn2r15	APN	5	109,441,240 (GRCm39)	missense	probably damaging	1.00
IGL02754:Vmn2r15	APN	5	109,441,134 (GRCm39)	nonsense	probably null
IGL02797:Vmn2r15	APN	5	109,445,250 (GRCm39)	missense	probably benign	0.18
IGL03301:Vmn2r15	APN	5	109,445,221 (GRCm39)	critical splice donor site	probably null
IGL03407:Vmn2r15	APN	5	109,434,185 (GRCm39)	nonsense	probably null
BB001:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
BB011:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r15	UTSW	5	109,435,008 (GRCm39)	missense	probably damaging	0.99
PIT4520001:Vmn2r15	UTSW	5	109,434,871 (GRCm39)	missense	probably damaging	1.00
R0038:Vmn2r15	UTSW	5	109,441,010 (GRCm39)	missense	possibly damaging	0.46
R0111:Vmn2r15	UTSW	5	109,435,022 (GRCm39)	missense	possibly damaging	0.56
R0379:Vmn2r15	UTSW	5	109,434,344 (GRCm39)	missense	probably damaging	1.00
R0427:Vmn2r15	UTSW	5	109,434,953 (GRCm39)	missense	probably damaging	1.00
R0639:Vmn2r15	UTSW	5	109,440,881 (GRCm39)	missense	probably benign	0.22
R0964:Vmn2r15	UTSW	5	109,445,401 (GRCm39)	missense	probably benign	0.34
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1232:Vmn2r15	UTSW	5	109,441,168 (GRCm39)	missense	probably benign	0.39
R1241:Vmn2r15	UTSW	5	109,440,770 (GRCm39)	missense	probably damaging	1.00
R1244:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R1394:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1395:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1423:Vmn2r15	UTSW	5	109,441,093 (GRCm39)	missense	probably damaging	1.00
R1439:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	probably damaging	1.00
R1513:Vmn2r15	UTSW	5	109,441,195 (GRCm39)	missense	probably damaging	1.00
R1777:Vmn2r15	UTSW	5	109,442,136 (GRCm39)	missense	possibly damaging	0.79
R1844:Vmn2r15	UTSW	5	109,434,860 (GRCm39)	nonsense	probably null
R2072:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2074:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2122:Vmn2r15	UTSW	5	109,434,322 (GRCm39)	missense	probably damaging	1.00
R2208:Vmn2r15	UTSW	5	109,445,309 (GRCm39)	missense	possibly damaging	0.64
R2268:Vmn2r15	UTSW	5	109,441,073 (GRCm39)	missense	probably benign	0.31
R2831:Vmn2r15	UTSW	5	109,434,458 (GRCm39)	missense	probably damaging	1.00
R3848:Vmn2r15	UTSW	5	109,445,312 (GRCm39)	missense	probably benign	0.00
R4058:Vmn2r15	UTSW	5	109,441,312 (GRCm39)	missense	probably damaging	0.99
R4615:Vmn2r15	UTSW	5	109,441,348 (GRCm39)	missense	possibly damaging	0.91
R4663:Vmn2r15	UTSW	5	109,441,940 (GRCm39)	missense	probably benign
R4751:Vmn2r15	UTSW	5	109,434,620 (GRCm39)	missense	probably benign	0.01
R5095:Vmn2r15	UTSW	5	109,436,317 (GRCm39)	critical splice acceptor site	probably null
R5300:Vmn2r15	UTSW	5	109,441,974 (GRCm39)	missense	probably damaging	0.99
R5309:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R5335:Vmn2r15	UTSW	5	109,434,673 (GRCm39)	missense	probably damaging	0.99
R5421:Vmn2r15	UTSW	5	109,434,401 (GRCm39)	missense	probably damaging	1.00
R5805:Vmn2r15	UTSW	5	109,434,806 (GRCm39)	missense	possibly damaging	0.88
R6280:Vmn2r15	UTSW	5	109,441,291 (GRCm39)	missense	possibly damaging	0.65
R6324:Vmn2r15	UTSW	5	109,434,137 (GRCm39)	makesense	probably null
R6383:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R6772:Vmn2r15	UTSW	5	109,434,238 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r15	UTSW	5	109,441,180 (GRCm39)	missense	probably damaging	1.00
R7194:Vmn2r15	UTSW	5	109,440,649 (GRCm39)	missense	probably damaging	1.00
R7365:Vmn2r15	UTSW	5	109,445,388 (GRCm39)	missense	probably benign	0.15
R7365:Vmn2r15	UTSW	5	109,441,105 (GRCm39)	missense	probably benign	0.19
R7423:Vmn2r15	UTSW	5	109,445,394 (GRCm39)	missense	probably benign	0.00
R7552:Vmn2r15	UTSW	5	109,440,774 (GRCm39)	nonsense	probably null
R7619:Vmn2r15	UTSW	5	109,436,190 (GRCm39)	critical splice donor site	probably null
R7892:Vmn2r15	UTSW	5	109,434,217 (GRCm39)	missense	probably damaging	1.00
R7924:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
R8058:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R8099:Vmn2r15	UTSW	5	109,441,185 (GRCm39)	missense	possibly damaging	0.58
R8189:Vmn2r15	UTSW	5	109,434,713 (GRCm39)	missense	probably benign	0.02
R8275:Vmn2r15	UTSW	5	109,434,150 (GRCm39)	missense	probably benign	0.08
R8277:Vmn2r15	UTSW	5	109,441,310 (GRCm39)	missense	probably damaging	1.00
R8277:Vmn2r15	UTSW	5	109,440,970 (GRCm39)	missense	probably benign	0.00
R8465:Vmn2r15	UTSW	5	109,445,302 (GRCm39)	missense	probably damaging	1.00
R8679:Vmn2r15	UTSW	5	109,434,779 (GRCm39)	missense	probably benign	0.31
R8682:Vmn2r15	UTSW	5	109,441,938 (GRCm39)	missense	probably damaging	0.98
R8809:Vmn2r15	UTSW	5	109,434,874 (GRCm39)	missense	probably benign	0.22
R9016:Vmn2r15	UTSW	5	109,442,109 (GRCm39)	missense	probably benign	0.01
R9372:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	possibly damaging	0.60
R9596:Vmn2r15	UTSW	5	109,440,791 (GRCm39)	missense	probably benign	0.01
R9619:Vmn2r15	UTSW	5	109,440,622 (GRCm39)	missense	possibly damaging	0.58
R9685:Vmn2r15	UTSW	5	109,440,598 (GRCm39)	missense	probably benign	0.05
R9716:Vmn2r15	UTSW	5	109,445,224 (GRCm39)	missense	possibly damaging	0.88
R9772:Vmn2r15	UTSW	5	109,434,923 (GRCm39)	missense	probably damaging	1.00
X0065:Vmn2r15	UTSW	5	109,441,174 (GRCm39)	missense	probably damaging	1.00
Z1187:Vmn2r15	UTSW	5	109,434,529 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTGATCCAGACACTGCAG -3'
(R):5'- TTCACAGTGGCTATTTCTACAGTG -3'

Sequencing Primer
(F):5'- TGCAGAATACCAGCATGCTG -3'
(R):5'- AACTGTGCTCATGGCTTTCAAG -3'

Posted On

2015-10-08