Incidental Mutation 'R4683:Wnt10a'
ID 350098
Institutional Source Beutler Lab
Gene Symbol Wnt10a
Ensembl Gene ENSMUSG00000026167
Gene Name wingless-type MMTV integration site family, member 10A
Synonyms
MMRRC Submission 041935-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R4683 (G1)
Quality Score 175
Status Not validated
Chromosome 1
Chromosomal Location 74831178-74843335 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 74842296 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Tyrosine at position 93 (H93Y)
Gene Model predicted gene model for transcript(s): [ENSMUST00000006718]
AlphaFold P70701
Predicted Effect silent
Transcript: ENSMUST00000006718
SMART Domains Protein: ENSMUSP00000006718
Gene: ENSMUSG00000026167

DomainStartEndE-ValueType
signal peptide 1 41 N/A INTRINSIC
WNT1 63 417 4.97e-143 SMART
Predicted Effect unknown
Transcript: ENSMUST00000187238
AA Change: H93Y
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.2%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit taurodontism, supernumerary molars, small molars and misshapened crowns. Mice homozygous for a conditional allele activated in Krt14+ cells also exhibit decreased basal cell proliferation affecting the tongue, sweat glands and nails. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 50 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ackr3 T C 1: 90,141,709 (GRCm39) V56A probably damaging Het
Acyp1 A G 12: 85,325,717 (GRCm39) probably benign Het
Adgrb1 A T 15: 74,459,963 (GRCm39) K532I probably damaging Het
Ahrr T A 13: 74,372,885 (GRCm39) silent Het
Asz1 T C 6: 18,055,541 (GRCm39) probably benign Het
AW554918 T C 18: 25,472,852 (GRCm39) Y219H probably benign Het
Ccno C A 13: 113,125,543 (GRCm39) probably null Het
Cdh17 A T 4: 11,817,036 (GRCm39) N816Y possibly damaging Het
Clca4a C T 3: 144,660,701 (GRCm39) V708I probably damaging Het
Col6a3 T A 1: 90,701,179 (GRCm39) Y2579F unknown Het
Col6a4 A G 9: 105,957,329 (GRCm39) V165A probably benign Het
Csf1r T A 18: 61,257,983 (GRCm39) C651S probably damaging Het
Cyp4f14 T C 17: 33,126,985 (GRCm39) D315G probably null Het
Def6 A G 17: 28,436,609 (GRCm39) D91G probably damaging Het
Dmxl1 T G 18: 50,011,088 (GRCm39) S1082A probably damaging Het
Dnah2 A T 11: 69,349,768 (GRCm39) Y2392N probably damaging Het
Dsg4 T C 18: 20,594,466 (GRCm39) S532P probably benign Het
Efr3a C A 15: 65,691,650 (GRCm39) S126R probably damaging Het
Gab1 A C 8: 81,515,261 (GRCm39) H352Q probably benign Het
Gm1110 A G 9: 26,831,890 (GRCm39) M87T probably damaging Het
Greb1 C T 12: 16,761,774 (GRCm39) M535I possibly damaging Het
Greb1l T A 18: 10,529,563 (GRCm39) probably null Het
Gucy2d T C 7: 98,102,650 (GRCm39) C487R probably benign Het
H1f7 A T 15: 98,154,921 (GRCm39) I76N probably damaging Het
Lrrc2 A T 9: 110,791,614 (GRCm39) H122L possibly damaging Het
Mrps22 A T 9: 98,480,359 (GRCm39) probably null Het
Mxd3 T C 13: 55,473,613 (GRCm39) T202A probably benign Het
Neb T C 2: 52,134,074 (GRCm39) H3303R possibly damaging Het
Nup133 G A 8: 124,657,721 (GRCm39) R405* probably null Het
Or13a19 T C 7: 139,902,681 (GRCm39) L23P probably benign Het
Or14j6 A G 17: 38,215,039 (GRCm39) T201A probably benign Het
Pard3b T C 1: 62,255,675 (GRCm39) Y629H probably benign Het
Pcnx1 A G 12: 82,033,446 (GRCm39) D1781G probably benign Het
Pcsk5 A T 19: 17,450,405 (GRCm39) C1148S probably damaging Het
Pcsk9 A T 4: 106,316,092 (GRCm39) I117N possibly damaging Het
Pcyt1b C A X: 92,789,970 (GRCm39) P318H probably damaging Het
Pfkfb2 T A 1: 130,634,221 (GRCm39) probably null Het
Pi4ka T C 16: 17,114,901 (GRCm39) E1456G possibly damaging Het
Rlig1 T C 10: 100,414,243 (GRCm39) I139V probably benign Het
Sh2b2 C T 5: 136,260,574 (GRCm39) C214Y probably damaging Het
Slc52a2 C A 15: 76,424,433 (GRCm39) P224T probably damaging Het
Slf2 C G 19: 44,923,920 (GRCm39) R245G probably benign Het
Sox5 T C 6: 143,779,193 (GRCm39) S648G probably damaging Het
Stk36 T A 1: 74,673,344 (GRCm39) I1079N probably benign Het
Stxbp3 T C 3: 108,708,188 (GRCm39) D371G probably damaging Het
Trnau1ap A T 4: 132,049,063 (GRCm39) Y47N probably damaging Het
Ubr5 C T 15: 38,038,211 (GRCm39) R316H probably damaging Het
Vmn1r230 C T 17: 21,067,515 (GRCm39) R235C probably benign Het
Zfp1005 T A 2: 150,108,390 (GRCm39) H50Q possibly damaging Het
Zfp52 A G 17: 21,781,769 (GRCm39) D539G probably benign Het
Other mutations in Wnt10a
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0347:Wnt10a UTSW 1 74,832,702 (GRCm39) missense probably damaging 0.97
R2312:Wnt10a UTSW 1 74,842,589 (GRCm39) missense possibly damaging 0.74
R3941:Wnt10a UTSW 1 74,842,656 (GRCm39) splice site probably null
R4719:Wnt10a UTSW 1 74,842,762 (GRCm39) missense probably damaging 1.00
R5218:Wnt10a UTSW 1 74,832,754 (GRCm39) missense probably benign
R5244:Wnt10a UTSW 1 74,842,454 (GRCm39) missense probably damaging 0.99
R5813:Wnt10a UTSW 1 74,839,755 (GRCm39) missense probably damaging 1.00
R6652:Wnt10a UTSW 1 74,842,613 (GRCm39) splice site probably null
R7278:Wnt10a UTSW 1 74,832,641 (GRCm39) missense possibly damaging 0.92
R7637:Wnt10a UTSW 1 74,832,633 (GRCm39) nonsense probably null
R8293:Wnt10a UTSW 1 74,842,376 (GRCm39) missense probably damaging 1.00
R9446:Wnt10a UTSW 1 74,842,728 (GRCm39) missense possibly damaging 0.52
Predicted Primers PCR Primer
(F):5'- CGCAGTTAGTTGCTAGAAAGGC -3'
(R):5'- CTCAAAGTAGACCAGGTCGGAG -3'

Sequencing Primer
(F):5'- GCCTAGCCGGATAGGAAGTG -3'
(R):5'- ACCAGGTCGGAGTGGCTG -3'
Posted On 2015-10-08