Mutagenetix > Incidental Mutation

Incidental Mutation 'R4645:Vmn2r74'

350168

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r74

Ensembl Gene

ENSMUSG00000090774

Gene Name

vomeronasal 2, receptor 74

Synonyms

EG546980

MMRRC Submission

041906-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R4645 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85601075-85610690 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 85606317 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 343 (S343N)

Ref Sequence

ENSEMBL: ENSMUSP00000126917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166355]

AlphaFold

E9PW21

Predicted Effect

probably benign
Transcript: ENSMUST00000166355
AA Change: S343N

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126917
Gene: ENSMUSG00000090774
AA Change: S343N

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	467	7.3e-28	PFAM
Pfam:NCD3G	510	562	4.7e-20	PFAM
Pfam:7tm_3	592	830	1.3e-52	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

98% (53/54)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	T	C	17: 46,635,700 (GRCm39)	E101G	probably damaging	Het
Adgrf5	A	G	17: 43,748,416 (GRCm39)	E29G	probably damaging	Het
Agfg2	T	A	5: 137,682,854 (GRCm39)		probably benign	Het
Ap4b1	A	G	3: 103,728,765 (GRCm39)	S468G	probably benign	Het
AY702103	G	T	17: 50,546,941 (GRCm39)		noncoding transcript	Het
Ccdc14	T	C	16: 34,542,110 (GRCm39)	L536S	probably damaging	Het
Ccr9	G	T	9: 123,608,658 (GRCm39)	M101I	probably benign	Het
Celsr1	A	T	15: 85,800,957 (GRCm39)	V2496E	probably benign	Het
Celsr2	C	A	3: 108,303,285 (GRCm39)	G2486V	probably damaging	Het
Cntnap3	A	G	13: 64,926,602 (GRCm39)		probably null	Het
Erlin1	T	C	19: 44,057,759 (GRCm39)	Y22C	probably damaging	Het
Fam135b	T	C	15: 71,334,189 (GRCm39)	T1002A	probably benign	Het
Gas2l3	CACTCGTCATACT	CACT	10: 89,266,820 (GRCm39)		probably benign	Het
Glud1	T	C	14: 34,033,063 (GRCm39)	V70A	probably damaging	Het
Hdlbp	A	G	1: 93,349,842 (GRCm39)		probably benign	Het
Immt	T	C	6: 71,833,923 (GRCm39)	L184P	probably damaging	Het
Iqsec1	T	G	6: 90,644,995 (GRCm39)	K983T	probably damaging	Het
Klhl6	A	T	16: 19,765,897 (GRCm39)	N568K	probably damaging	Het
Lbhd1	T	G	19: 8,861,452 (GRCm39)		probably benign	Het
Lrit2	C	T	14: 36,794,432 (GRCm39)	R499C	probably benign	Het
Lrp6	T	C	6: 134,461,213 (GRCm39)	D748G	probably damaging	Het
Lrriq4	A	G	3: 30,704,892 (GRCm39)	K292E	probably benign	Het
Ly96	G	T	1: 16,761,940 (GRCm39)	E49*	probably null	Het
Lztr1	C	T	16: 17,341,955 (GRCm39)		probably benign	Het
Mmp12	A	G	9: 7,347,515 (GRCm39)	M31V	probably benign	Het
Mok	A	T	12: 110,774,873 (GRCm39)		probably benign	Het
Mpi	T	C	9: 57,458,040 (GRCm39)	H54R	probably damaging	Het
Naip5	T	A	13: 100,356,338 (GRCm39)	E1092D	probably benign	Het
Or4c101	T	C	2: 88,390,722 (GRCm39)	I292T	probably damaging	Het
Pomt1	G	T	2: 32,132,888 (GRCm39)		probably benign	Het
Ptpn20	G	A	14: 33,353,169 (GRCm39)	V303I	probably benign	Het
Pyroxd1	C	G	6: 142,300,467 (GRCm39)	S199*	probably null	Het
Shank2	A	G	7: 143,964,159 (GRCm39)	K799R	possibly damaging	Het
Sim1	A	T	10: 50,860,093 (GRCm39)	T652S	probably benign	Het
Slc8a2	C	T	7: 15,868,164 (GRCm39)	T132I	probably damaging	Het
Spaca6	A	T	17: 18,056,307 (GRCm39)		probably benign	Het
Sympk	G	T	7: 18,777,385 (GRCm39)	R545L	possibly damaging	Het
Tenm4	G	T	7: 96,544,949 (GRCm39)	G2322W	probably damaging	Het
Thap1	A	G	8: 26,652,597 (GRCm39)	T135A	probably damaging	Het
Traf3	T	C	12: 111,228,400 (GRCm39)	V537A	probably damaging	Het
Ttn	T	C	2: 76,578,680 (GRCm39)	D24071G	probably damaging	Het
Tysnd1	G	T	10: 61,531,962 (GRCm39)	V205L	probably benign	Het
Unc79	T	C	12: 103,079,081 (GRCm39)	S1749P	probably benign	Het
Vmn2r50	T	A	7: 9,771,162 (GRCm39)	*846C	probably null	Het
Zfyve28	T	G	5: 34,379,787 (GRCm39)		probably benign	Het
Zmym2	T	C	14: 57,165,764 (GRCm39)	S696P	probably damaging	Het
Zscan4e	T	C	7: 11,041,002 (GRCm39)	Y290C	possibly damaging	Het
Zswim2	G	T	2: 83,745,891 (GRCm39)	H516N	probably benign	Het

Other mutations in Vmn2r74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Vmn2r74	APN	7	85,606,338 (GRCm39)	missense	probably benign	0.03
IGL00904:Vmn2r74	APN	7	85,606,788 (GRCm39)	missense	probably benign	0.05
IGL01285:Vmn2r74	APN	7	85,606,692 (GRCm39)	missense	possibly damaging	0.54
IGL01300:Vmn2r74	APN	7	85,606,414 (GRCm39)	missense	probably benign	0.00
IGL01410:Vmn2r74	APN	7	85,610,500 (GRCm39)	missense	possibly damaging	0.83
IGL01827:Vmn2r74	APN	7	85,606,800 (GRCm39)	missense	probably benign	0.00
IGL02094:Vmn2r74	APN	7	85,610,669 (GRCm39)	missense	probably benign	0.01
IGL02252:Vmn2r74	APN	7	85,606,531 (GRCm39)	missense	probably benign	0.41
IGL02349:Vmn2r74	APN	7	85,601,724 (GRCm39)	missense	probably damaging	0.99
IGL02438:Vmn2r74	APN	7	85,601,824 (GRCm39)	missense	probably damaging	0.98
IGL02554:Vmn2r74	APN	7	85,606,581 (GRCm39)	missense	probably benign	0.00
IGL03036:Vmn2r74	APN	7	85,601,900 (GRCm39)	nonsense	probably null
IGL03370:Vmn2r74	APN	7	85,607,265 (GRCm39)	missense	probably benign
R0115:Vmn2r74	UTSW	7	85,606,564 (GRCm39)	missense	probably benign	0.00
R0333:Vmn2r74	UTSW	7	85,601,491 (GRCm39)	missense	probably benign	0.06
R0415:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R0571:Vmn2r74	UTSW	7	85,601,629 (GRCm39)	missense	probably damaging	1.00
R0626:Vmn2r74	UTSW	7	85,610,517 (GRCm39)	nonsense	probably null
R0659:Vmn2r74	UTSW	7	85,605,122 (GRCm39)	splice site	probably benign
R1202:Vmn2r74	UTSW	7	85,610,545 (GRCm39)	missense	possibly damaging	0.83
R1473:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R1908:Vmn2r74	UTSW	7	85,601,650 (GRCm39)	missense	probably benign
R2079:Vmn2r74	UTSW	7	85,606,383 (GRCm39)	missense	probably benign	0.00
R2368:Vmn2r74	UTSW	7	85,610,522 (GRCm39)	missense	probably benign	0.39
R3782:Vmn2r74	UTSW	7	85,605,322 (GRCm39)	missense	probably benign	0.01
R3824:Vmn2r74	UTSW	7	85,607,466 (GRCm39)	missense	probably damaging	1.00
R3977:Vmn2r74	UTSW	7	85,607,345 (GRCm39)	missense	probably benign	0.01
R4182:Vmn2r74	UTSW	7	85,606,395 (GRCm39)	missense	possibly damaging	0.87
R4289:Vmn2r74	UTSW	7	85,606,562 (GRCm39)	missense	probably benign
R4294:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R4646:Vmn2r74	UTSW	7	85,606,782 (GRCm39)	missense	probably benign	0.42
R4655:Vmn2r74	UTSW	7	85,610,555 (GRCm39)	missense	probably benign
R4901:Vmn2r74	UTSW	7	85,605,199 (GRCm39)	nonsense	probably null
R5532:Vmn2r74	UTSW	7	85,601,197 (GRCm39)	missense	probably benign	0.32
R5642:Vmn2r74	UTSW	7	85,606,588 (GRCm39)	missense	probably benign	0.00
R5913:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6170:Vmn2r74	UTSW	7	85,606,348 (GRCm39)	missense	probably benign	0.03
R6232:Vmn2r74	UTSW	7	85,607,498 (GRCm39)	missense	possibly damaging	0.82
R6238:Vmn2r74	UTSW	7	85,601,280 (GRCm39)	missense	probably damaging	1.00
R6255:Vmn2r74	UTSW	7	85,601,659 (GRCm39)	missense	possibly damaging	0.90
R6468:Vmn2r74	UTSW	7	85,610,599 (GRCm39)	missense	probably benign	0.34
R6732:Vmn2r74	UTSW	7	85,606,758 (GRCm39)	missense	probably damaging	1.00
R6816:Vmn2r74	UTSW	7	85,610,621 (GRCm39)	nonsense	probably null
R6836:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.00
R6995:Vmn2r74	UTSW	7	85,606,860 (GRCm39)	critical splice acceptor site	probably null
R6995:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R7186:Vmn2r74	UTSW	7	85,601,150 (GRCm39)	nonsense	probably null
R7246:Vmn2r74	UTSW	7	85,605,173 (GRCm39)	missense	probably benign
R7374:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.02
R7505:Vmn2r74	UTSW	7	85,606,279 (GRCm39)	nonsense	probably null
R7525:Vmn2r74	UTSW	7	85,610,510 (GRCm39)	missense	probably benign
R7569:Vmn2r74	UTSW	7	85,601,544 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r74	UTSW	7	85,606,746 (GRCm39)	missense	probably benign	0.11
R7956:Vmn2r74	UTSW	7	85,605,166 (GRCm39)	missense	probably benign	0.09
R8119:Vmn2r74	UTSW	7	85,610,690 (GRCm39)	start codon destroyed	probably null	0.08
R8131:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R8147:Vmn2r74	UTSW	7	85,605,227 (GRCm39)	nonsense	probably null
R8181:Vmn2r74	UTSW	7	85,605,324 (GRCm39)	missense	probably damaging	1.00
R8184:Vmn2r74	UTSW	7	85,601,454 (GRCm39)	missense	probably benign	0.00
R8375:Vmn2r74	UTSW	7	85,601,914 (GRCm39)	missense	possibly damaging	0.64
R8948:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R9033:Vmn2r74	UTSW	7	85,606,414 (GRCm39)	missense	probably benign
R9342:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R9578:Vmn2r74	UTSW	7	85,606,101 (GRCm39)	missense	probably benign	0.01
R9607:Vmn2r74	UTSW	7	85,610,619 (GRCm39)	missense	probably benign	0.02
R9776:Vmn2r74	UTSW	7	85,605,212 (GRCm39)	missense	possibly damaging	0.70
Z1176:Vmn2r74	UTSW	7	85,604,835 (GRCm39)	missense	probably damaging	1.00
Z31818:Vmn2r74	UTSW	7	85,604,729 (GRCm39)	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- TGCAAAAGCATCCCATGGAG -3'
(R):5'- CTATGCCTTCATTGTGTGGAAATC -3'

Sequencing Primer
(F):5'- GGCCACAGCATACATAGCGTTG -3'
(R):5'- CTCAAGACCTTTTCAGAATCTGGG -3'

Posted On

2015-10-08