Mutagenetix > Incidental Mutation

Incidental Mutation 'R4649:Zfat'

350636

Institutional Source

Beutler Lab

Gene Symbol

Zfat

Ensembl Gene

ENSMUSG00000022335

Gene Name

zinc finger and AT hook domain containing

Synonyms

LOC380993, Zfp406, Zfat1

MMRRC Submission

041910-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4649 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67955613-68130705 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 68056325 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 237 (Y237H)

Ref Sequence

ENSEMBL: ENSMUSP00000125732 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159430] [ENSMUST00000160248] [ENSMUST00000162054] [ENSMUST00000162173]

AlphaFold

Q7TS63

PDB Structure

Solution structure of the 5th C2H2 zinc finger of mouse Zinc finger protein 406 [SOLUTION NMR]
Solution structure of the 8th C2H2 zinc finger of mouse Zinc finger protein 406 [SOLUTION NMR]

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159430

SMART Domains

Protein: ENSMUSP00000124420
Gene: ENSMUSG00000022335

Domain	Start	End	E-Value	Type
ZnF_C2H2	12	35	2.45e0	SMART
ZnF_C2H2	116	136	1.53e2	SMART
low complexity region	158	174	N/A	INTRINSIC
ZnF_C2H2	209	231	1.47e-3	SMART
ZnF_C2H2	237	259	1.18e-2	SMART
ZnF_C2H2	264	287	2.36e-2	SMART
ZnF_C2H2	292	315	4.4e-2	SMART
ZnF_C2H2	342	364	1.67e-2	SMART
ZnF_C2H2	370	392	1.33e-1	SMART
ZnF_C2H2	396	419	2.05e-2	SMART
low complexity region	539	555	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000160248
AA Change: Y244H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000125257
Gene: ENSMUSG00000022335
AA Change: Y244H

Domain	Start	End	E-Value	Type
ZnF_C2H2	12	35	2.45e0	SMART
ZnF_C2H2	116	136	1.53e2	SMART
low complexity region	220	236	N/A	INTRINSIC
ZnF_C2H2	271	293	1.47e-3	SMART
ZnF_C2H2	299	321	1.18e-2	SMART
ZnF_C2H2	326	349	2.36e-2	SMART
ZnF_C2H2	354	377	4.4e-2	SMART
ZnF_C2H2	404	426	1.67e-2	SMART
ZnF_C2H2	432	454	1.33e-1	SMART
ZnF_C2H2	458	481	2.05e-2	SMART
low complexity region	601	617	N/A	INTRINSIC
ZnF_C2H2	737	759	1.43e-1	SMART
ZnF_C2H2	765	788	3.52e-1	SMART
ZnF_C2H2	793	817	5.59e-4	SMART
ZnF_C2H2	825	848	3.83e-2	SMART
ZnF_C2H2	875	898	3.95e1	SMART
ZnF_C2H2	904	926	6.88e-4	SMART
ZnF_C2H2	932	954	8.94e-3	SMART
ZnF_C2H2	961	983	2.27e-4	SMART
ZnF_C2H2	989	1012	9.3e-1	SMART
ZnF_C2H2	1036	1059	5.21e-4	SMART
low complexity region	1073	1082	N/A	INTRINSIC
low complexity region	1139	1149	N/A	INTRINSIC
low complexity region	1218	1236	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162054
AA Change: Y237H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125732
Gene: ENSMUSG00000022335
AA Change: Y237H

Domain	Start	End	E-Value	Type
ZnF_C2H2	12	35	2.45e0	SMART
ZnF_C2H2	116	136	1.53e2	SMART
low complexity region	213	229	N/A	INTRINSIC
ZnF_C2H2	264	286	1.47e-3	SMART
ZnF_C2H2	292	314	1.18e-2	SMART
ZnF_C2H2	319	342	2.36e-2	SMART
ZnF_C2H2	347	370	4.4e-2	SMART
ZnF_C2H2	397	419	1.67e-2	SMART
ZnF_C2H2	425	447	1.33e-1	SMART
ZnF_C2H2	451	474	2.05e-2	SMART
low complexity region	594	610	N/A	INTRINSIC
ZnF_C2H2	730	752	1.43e-1	SMART
ZnF_C2H2	758	781	3.52e-1	SMART
ZnF_C2H2	786	810	5.59e-4	SMART
ZnF_C2H2	818	841	3.83e-2	SMART
ZnF_C2H2	868	891	3.95e1	SMART
ZnF_C2H2	897	919	6.88e-4	SMART
ZnF_C2H2	925	947	8.94e-3	SMART
ZnF_C2H2	954	976	2.27e-4	SMART
ZnF_C2H2	982	1005	9.3e-1	SMART
ZnF_C2H2	1029	1052	5.21e-4	SMART
low complexity region	1121	1131	N/A	INTRINSIC
low complexity region	1200	1218	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162173
AA Change: Y244H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124974
Gene: ENSMUSG00000022335
AA Change: Y244H

Domain	Start	End	E-Value	Type
ZnF_C2H2	12	35	2.45e0	SMART
ZnF_C2H2	116	136	1.53e2	SMART
low complexity region	220	236	N/A	INTRINSIC
ZnF_C2H2	271	293	1.47e-3	SMART
ZnF_C2H2	299	321	1.18e-2	SMART
ZnF_C2H2	326	349	2.36e-2	SMART
ZnF_C2H2	354	377	4.4e-2	SMART
ZnF_C2H2	404	426	1.67e-2	SMART
ZnF_C2H2	432	454	1.33e-1	SMART
ZnF_C2H2	458	481	2.05e-2	SMART
low complexity region	601	617	N/A	INTRINSIC
ZnF_C2H2	737	759	1.43e-1	SMART
ZnF_C2H2	765	788	3.52e-1	SMART
ZnF_C2H2	793	817	5.59e-4	SMART
ZnF_C2H2	825	848	3.83e-2	SMART
ZnF_C2H2	875	898	3.95e1	SMART
ZnF_C2H2	904	926	6.88e-4	SMART
ZnF_C2H2	932	954	8.94e-3	SMART
ZnF_C2H2	961	983	2.27e-4	SMART
ZnF_C2H2	989	1012	9.3e-1	SMART
ZnF_C2H2	1036	1059	5.21e-4	SMART
low complexity region	1133	1151	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that likely binds DNA and functions as a transcriptional regulator involved in apoptosis and cell survival. This gene resides in a susceptibility locus for autoimmune thyroid disease (AITD) on chromosome 8q24. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit complete embryonic lethality associated with failure to initiation of embryo turning, abnormal embryonic hematopoiesis, abnormal spongiotrophoblast layer morphology, abnormal visceral yolk sac blood island morphology and pale yolk sac. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
4933409G03Rik	G	A	2: 68,436,559 (GRCm39)	E168K	unknown	Het
Abca4	G	T	3: 121,963,542 (GRCm39)	V841F	probably damaging	Het
Adcy10	G	A	1: 165,331,618 (GRCm39)	E84K	probably damaging	Het
Adgrg6	C	A	10: 14,344,571 (GRCm39)	M127I	probably damaging	Het
Agpat5	A	G	8: 18,929,668 (GRCm39)	I266V	possibly damaging	Het
Agtpbp1	A	T	13: 59,676,213 (GRCm39)	Y186N	possibly damaging	Het
Alx1	A	T	10: 102,845,193 (GRCm39)	N279K	probably damaging	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Arsi	A	G	18: 61,050,170 (GRCm39)	Y351C	probably damaging	Het
B4galnt3	T	A	6: 120,201,581 (GRCm39)	R155W	probably damaging	Het
BC028528	T	C	3: 95,795,577 (GRCm39)	E75G	probably damaging	Het
Bmp2k	C	T	5: 97,200,970 (GRCm39)	P297S	possibly damaging	Het
Btnl4	A	G	17: 34,691,602 (GRCm39)	S296P	probably benign	Het
Bzw2	A	T	12: 36,173,980 (GRCm39)	V98E	probably null	Het
Cacna1d	C	T	14: 29,817,365 (GRCm39)	M1232I	probably benign	Het
Cast	A	G	13: 74,894,133 (GRCm39)	S171P	probably benign	Het
Catsperb	G	A	12: 101,507,771 (GRCm39)	A513T	probably benign	Het
Cd19	A	G	7: 126,013,664 (GRCm39)	S42P	probably benign	Het
Chml	A	G	1: 175,514,962 (GRCm39)	S138P	probably benign	Het
Clic6	T	G	16: 92,327,827 (GRCm39)		probably null	Het
Col12a1	A	T	9: 79,547,076 (GRCm39)	V2252E	probably damaging	Het
Cplx2	G	A	13: 54,527,361 (GRCm39)	E82K	probably benign	Het
Csmd2	G	A	4: 128,439,866 (GRCm39)	V3107M	probably benign	Het
Cspg4	T	A	9: 56,794,149 (GRCm39)	V628D	possibly damaging	Het
Dcaf13	G	A	15: 39,001,637 (GRCm39)	R315Q	possibly damaging	Het
Deaf1	A	G	7: 140,877,486 (GRCm39)	V547A	possibly damaging	Het
Dmxl1	C	T	18: 50,011,698 (GRCm39)	T1285I	probably damaging	Het
Dnah3	C	A	7: 119,646,921 (GRCm39)	W1036L	probably damaging	Het
Dnai3	A	G	3: 145,753,922 (GRCm39)	S713P	probably damaging	Het
Dpy19l1	A	C	9: 24,393,350 (GRCm39)	S140A	possibly damaging	Het
Dsg2	T	A	18: 20,735,302 (GRCm39)	D1093E	possibly damaging	Het
Edaradd	T	A	13: 12,523,304 (GRCm39)	H12L	probably damaging	Het
Enam	T	C	5: 88,640,827 (GRCm39)	M163T	probably benign	Het
Fbxo22	T	A	9: 55,128,333 (GRCm39)	V218E	probably damaging	Het
Fbxo34	A	T	14: 47,767,085 (GRCm39)	K148N	probably damaging	Het
Foxi3	A	G	6: 70,933,960 (GRCm39)	D149G	probably damaging	Het
Gbp8	C	T	5: 105,189,807 (GRCm39)	G75D	probably damaging	Het
Gm10784	C	T	13: 50,099,251 (GRCm39)		noncoding transcript	Het
Gm7247	G	A	14: 51,807,051 (GRCm39)		probably null	Het
Grik3	T	A	4: 125,544,278 (GRCm39)	V333E	probably damaging	Het
Gsap	A	T	5: 21,431,309 (GRCm39)	D211V	probably damaging	Het
H2-T15	T	C	17: 36,368,768 (GRCm39)	Y104C	possibly damaging	Het
Ino80	T	C	2: 119,261,489 (GRCm39)	N725S	probably damaging	Het
Itgad	A	G	7: 127,788,703 (GRCm39)	I443V	probably benign	Het
Itsn1	T	A	16: 91,638,476 (GRCm39)	I52N	probably damaging	Het
Kmt2b	TCTCCTCCTCCTCCTCCTCCTCCTCC	TCTCCTCCTCCTCCTCCTCCTCC	7: 30,285,783 (GRCm39)		probably benign	Het
Lamc1	T	G	1: 153,104,523 (GRCm39)	S59R	probably damaging	Het
Lrrk1	T	C	7: 65,922,801 (GRCm39)	I1366V	probably benign	Het
Lyn	C	A	4: 3,738,850 (GRCm39)	Q41K	probably benign	Het
Mapk13	C	T	17: 28,997,461 (GRCm39)	R360*	probably null	Het
Mapk8ip3	T	A	17: 25,123,726 (GRCm39)	I599F	probably damaging	Het
Me1	T	C	9: 86,561,905 (GRCm39)	K60R	probably benign	Het
Mga	T	A	2: 119,771,974 (GRCm39)	D1532E	possibly damaging	Het
Miga1	G	A	3: 151,984,642 (GRCm39)	S427L	probably benign	Het
Mknk2	A	T	10: 80,505,173 (GRCm39)	V186E	probably damaging	Het
Myo1a	C	A	10: 127,546,086 (GRCm39)	N236K	probably benign	Het
Myt1	A	G	2: 181,439,207 (GRCm39)	D243G	probably benign	Het
Ncf4	T	C	15: 78,140,189 (GRCm39)	V196A	possibly damaging	Het
Nlrp5	G	T	7: 23,117,603 (GRCm39)	L442F	probably damaging	Het
Olfm4	G	A	14: 80,258,747 (GRCm39)	D332N	probably benign	Het
Or4a81	C	A	2: 89,619,637 (GRCm39)	V20F	probably benign	Het
Or4b1d	T	A	2: 89,969,432 (GRCm39)	D17V	probably damaging	Het
Osbpl11	T	A	16: 33,016,452 (GRCm39)	M69K	probably benign	Het
Oxnad1	T	A	14: 31,824,366 (GRCm39)	*312K	probably null	Het
Plekhg1	A	G	10: 3,906,985 (GRCm39)	Q634R	probably benign	Het
Plekhh2	T	C	17: 84,882,691 (GRCm39)	Y733H	probably damaging	Het
Ppp1r21	A	G	17: 88,856,941 (GRCm39)	H132R	probably benign	Het
Prkcq	T	C	2: 11,284,333 (GRCm39)	V501A	possibly damaging	Het
Prpf38b	G	A	3: 108,811,408 (GRCm39)		probably benign	Het
Prpf4b	A	T	13: 35,083,954 (GRCm39)	M908L	probably benign	Het
Prps2	T	C	X: 166,135,288 (GRCm39)	D183G	probably damaging	Het
Pudp	T	C	18: 50,701,259 (GRCm39)	D158G	probably damaging	Het
R3hdm1	T	C	1: 128,112,181 (GRCm39)	S422P	probably damaging	Het
Ranbp3	T	A	17: 57,003,640 (GRCm39)		probably null	Het
Ranbp6	A	G	19: 29,787,721 (GRCm39)	V877A	probably benign	Het
Rgsl1	A	T	1: 153,693,328 (GRCm39)	S618T	probably benign	Het
Rhox2a	G	C	X: 36,508,962 (GRCm39)	R43P	probably benign	Het
Rhox2a	A	T	X: 36,509,015 (GRCm39)	I61L	probably benign	Het
Rnf207	A	C	4: 152,396,612 (GRCm39)	D466E	probably benign	Het
Rnf7	A	G	9: 96,353,883 (GRCm39)	I80T	probably benign	Het
Ros1	T	A	10: 52,005,764 (GRCm39)	I878F	possibly damaging	Het
Rsbn1	T	A	3: 103,861,096 (GRCm39)		probably null	Het
Sbk1	T	C	7: 125,890,301 (GRCm39)	S105P	probably damaging	Het
Sdk1	T	C	5: 141,992,380 (GRCm39)	Y790H	probably damaging	Het
Sec62	T	A	3: 30,864,683 (GRCm39)	N182K	unknown	Het
Slc15a1	T	C	14: 121,715,504 (GRCm39)	Y345C	probably damaging	Het
Slc27a1	G	A	8: 72,023,408 (GRCm39)	A15T	probably benign	Het
Slc35d1	A	G	4: 103,070,426 (GRCm39)	F110L	probably damaging	Het
Slc39a5	T	A	10: 128,233,136 (GRCm39)	S333C	probably benign	Het
Tarbp1	A	G	8: 127,173,934 (GRCm39)	S927P	probably damaging	Het
Tns1	A	G	1: 73,992,930 (GRCm39)	S583P	probably damaging	Het
Trim38	A	T	13: 23,966,952 (GRCm39)	D133V	probably damaging	Het
Trim40	T	C	17: 37,193,531 (GRCm39)		probably null	Het
Trim42	C	A	9: 97,244,998 (GRCm39)	V601L	probably benign	Het
Trpc7	G	A	13: 57,035,367 (GRCm39)	R189C	probably damaging	Het
Ttc29	A	T	8: 79,060,208 (GRCm39)	Y376F	probably benign	Het
Vmn2r63	A	G	7: 42,553,114 (GRCm39)	I714T	possibly damaging	Het
Vmn2r68	C	A	7: 84,870,743 (GRCm39)	A847S	probably benign	Het
Zbp1	G	A	2: 173,049,608 (GRCm39)	P385S	possibly damaging	Het
Zfp236	A	T	18: 82,615,784 (GRCm39)	W1840R	probably damaging	Het
Zfp426	G	T	9: 20,381,923 (GRCm39)	Q341K	possibly damaging	Het
Zfp462	T	A	4: 55,009,349 (GRCm39)	N438K	probably benign	Het
Zfp952	T	A	17: 33,221,899 (GRCm39)	V88E	probably damaging	Het
Znfx1	G	T	2: 166,898,276 (GRCm39)	A216D	probably benign	Het

Other mutations in Zfat

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00542:Zfat	APN	15	68,042,071 (GRCm39)	missense	possibly damaging	0.92
IGL00862:Zfat	APN	15	68,130,512 (GRCm39)	splice site	probably null
IGL01021:Zfat	APN	15	68,042,015 (GRCm39)	missense	possibly damaging	0.50
IGL01152:Zfat	APN	15	67,982,353 (GRCm39)	missense	probably damaging	1.00
IGL01733:Zfat	APN	15	68,052,579 (GRCm39)	missense	probably damaging	1.00
IGL01873:Zfat	APN	15	68,096,744 (GRCm39)	missense	probably benign	0.00
IGL01990:Zfat	APN	15	68,096,666 (GRCm39)	missense	probably damaging	1.00
IGL02066:Zfat	APN	15	68,052,678 (GRCm39)	missense	probably damaging	1.00
IGL02664:Zfat	APN	15	68,052,570 (GRCm39)	missense	probably damaging	1.00
IGL02955:Zfat	APN	15	68,052,963 (GRCm39)	missense	probably damaging	0.98
IGL03201:Zfat	APN	15	68,037,758 (GRCm39)	missense	probably damaging	1.00
R0145:Zfat	UTSW	15	68,058,948 (GRCm39)	missense	possibly damaging	0.95
R0408:Zfat	UTSW	15	68,052,141 (GRCm39)	missense	probably benign	0.10
R0633:Zfat	UTSW	15	68,052,652 (GRCm39)	missense	probably damaging	1.00
R1147:Zfat	UTSW	15	68,084,432 (GRCm39)	splice site	probably benign
R1508:Zfat	UTSW	15	68,050,600 (GRCm39)	missense	probably damaging	1.00
R1513:Zfat	UTSW	15	68,084,529 (GRCm39)	missense	probably damaging	1.00
R1641:Zfat	UTSW	15	68,051,959 (GRCm39)	missense	probably benign	0.19
R1889:Zfat	UTSW	15	67,973,388 (GRCm39)	missense	probably benign	0.00
R1959:Zfat	UTSW	15	68,018,392 (GRCm39)	missense	probably benign	0.32
R2030:Zfat	UTSW	15	67,990,783 (GRCm39)	critical splice donor site	probably null
R2202:Zfat	UTSW	15	68,051,709 (GRCm39)	missense	probably benign	0.36
R2340:Zfat	UTSW	15	67,973,390 (GRCm39)	missense	probably damaging	0.99
R3440:Zfat	UTSW	15	67,956,402 (GRCm39)	missense	probably benign	0.00
R3442:Zfat	UTSW	15	67,973,430 (GRCm39)	missense	probably damaging	0.99
R3442:Zfat	UTSW	15	67,956,402 (GRCm39)	missense	probably benign	0.00
R4406:Zfat	UTSW	15	68,052,040 (GRCm39)	missense	probably benign	0.00
R4710:Zfat	UTSW	15	68,052,131 (GRCm39)	missense	probably benign
R4712:Zfat	UTSW	15	67,982,324 (GRCm39)	critical splice donor site	probably null
R4745:Zfat	UTSW	15	68,052,223 (GRCm39)	missense	probably benign	0.09
R4862:Zfat	UTSW	15	68,051,959 (GRCm39)	missense	probably benign	0.02
R5015:Zfat	UTSW	15	68,050,762 (GRCm39)	missense	probably damaging	1.00
R5075:Zfat	UTSW	15	68,052,079 (GRCm39)	missense	probably benign
R5208:Zfat	UTSW	15	68,052,570 (GRCm39)	missense	probably damaging	1.00
R5277:Zfat	UTSW	15	68,037,758 (GRCm39)	missense	probably damaging	1.00
R5303:Zfat	UTSW	15	67,982,335 (GRCm39)	missense	probably damaging	1.00
R5328:Zfat	UTSW	15	68,051,677 (GRCm39)	missense	probably damaging	0.99
R5642:Zfat	UTSW	15	68,052,765 (GRCm39)	missense	probably damaging	1.00
R5659:Zfat	UTSW	15	67,990,862 (GRCm39)	missense	probably damaging	1.00
R5947:Zfat	UTSW	15	68,051,806 (GRCm39)	missense	probably benign
R6046:Zfat	UTSW	15	68,052,626 (GRCm39)	missense	probably damaging	0.99
R6315:Zfat	UTSW	15	67,956,311 (GRCm39)	missense	probably damaging	1.00
R6342:Zfat	UTSW	15	68,052,831 (GRCm39)	missense	probably damaging	1.00
R6573:Zfat	UTSW	15	68,037,703 (GRCm39)	missense	probably damaging	1.00
R6789:Zfat	UTSW	15	67,956,235 (GRCm39)	missense	probably damaging	1.00
R7028:Zfat	UTSW	15	68,052,301 (GRCm39)	missense	probably damaging	1.00
R7033:Zfat	UTSW	15	68,052,864 (GRCm39)	missense	probably damaging	1.00
R7039:Zfat	UTSW	15	68,052,211 (GRCm39)	missense	probably benign
R7065:Zfat	UTSW	15	68,052,969 (GRCm39)	missense	probably damaging	1.00
R7144:Zfat	UTSW	15	68,050,631 (GRCm39)	missense	probably benign	0.12
R7208:Zfat	UTSW	15	68,051,856 (GRCm39)	missense	probably benign	0.39
R7330:Zfat	UTSW	15	68,084,600 (GRCm39)	missense	probably benign	0.00
R7345:Zfat	UTSW	15	67,976,892 (GRCm39)	missense	probably damaging	1.00
R7378:Zfat	UTSW	15	68,052,969 (GRCm39)	missense	probably damaging	1.00
R7405:Zfat	UTSW	15	68,056,334 (GRCm39)	missense	probably damaging	1.00
R7481:Zfat	UTSW	15	68,050,715 (GRCm39)	nonsense	probably null
R7672:Zfat	UTSW	15	68,130,535 (GRCm39)	start codon destroyed	probably null	0.39
R7676:Zfat	UTSW	15	68,096,693 (GRCm39)	missense	possibly damaging	0.88
R7701:Zfat	UTSW	15	68,052,757 (GRCm39)	nonsense	probably null
R7825:Zfat	UTSW	15	68,051,769 (GRCm39)	missense	probably benign	0.01
R8152:Zfat	UTSW	15	67,973,355 (GRCm39)	missense	probably benign	0.23
R8404:Zfat	UTSW	15	67,976,916 (GRCm39)	missense	probably benign	0.00
R8405:Zfat	UTSW	15	68,018,410 (GRCm39)	missense	probably damaging	1.00
R8502:Zfat	UTSW	15	67,976,916 (GRCm39)	missense	probably benign	0.00
R8534:Zfat	UTSW	15	68,037,696 (GRCm39)	missense	probably damaging	1.00
R8708:Zfat	UTSW	15	67,956,278 (GRCm39)	missense	possibly damaging	0.95
R8887:Zfat	UTSW	15	68,056,315 (GRCm39)	missense	probably damaging	1.00
R8896:Zfat	UTSW	15	68,052,519 (GRCm39)	missense	probably damaging	1.00
R8906:Zfat	UTSW	15	67,956,404 (GRCm39)	missense	possibly damaging	0.81
R9117:Zfat	UTSW	15	68,058,918 (GRCm39)	missense	probably damaging	0.98
R9137:Zfat	UTSW	15	68,051,794 (GRCm39)	missense	probably benign	0.00
R9310:Zfat	UTSW	15	67,956,250 (GRCm39)	missense	probably damaging	1.00
R9482:Zfat	UTSW	15	68,084,652 (GRCm39)	missense	probably damaging	1.00
R9610:Zfat	UTSW	15	68,051,655 (GRCm39)	missense	possibly damaging	0.58
R9611:Zfat	UTSW	15	68,051,655 (GRCm39)	missense	possibly damaging	0.58
R9630:Zfat	UTSW	15	67,990,793 (GRCm39)	missense	probably benign	0.37
Z1088:Zfat	UTSW	15	68,058,950 (GRCm39)	missense	probably benign	0.00
Z1177:Zfat	UTSW	15	68,051,677 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCTAAGCTAATGGGTCAAGACTG -3'
(R):5'- TGTTGCATGGAAGTCCTACAG -3'

Sequencing Primer
(F):5'- TGGGTCAAGACTGTATAGATAAGGTC -3'
(R):5'- TGGAAGTCCTACAGTAAAATGCC -3'

Posted On

2015-10-08