Mutagenetix > Incidental Mutation

Incidental Mutation 'R4661:Muc4'

352887

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

041600-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4661 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32555089-32602761 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 32589651 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Alanine at position 2885 (E2885A)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096106
AA Change: E2885A

PolyPhen 2 Score 0.707 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: E2885A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753
AA Change: E815A

PolyPhen 2 Score 0.136 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620
AA Change: E815A

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg4	T	C	9: 44,198,627 (GRCm39)	N42D	probably damaging	Het
Adamdec1	G	A	14: 68,807,562 (GRCm39)	T366I	probably damaging	Het
Adamts7	C	A	9: 90,075,383 (GRCm39)	H1038Q	probably benign	Het
Aff3	T	C	1: 38,666,209 (GRCm39)	D5G	possibly damaging	Het
Amhr2	A	T	15: 102,362,688 (GRCm39)	D485V	probably damaging	Het
Arhgap35	A	T	7: 16,298,663 (GRCm39)	F134Y	probably damaging	Het
Asxl3	A	G	18: 22,649,534 (GRCm39)	T508A	probably benign	Het
Atp10a	TGGCGGCGGC	TGGCGGC	7: 58,308,248 (GRCm39)		probably benign	Het
Atp4a	G	A	7: 30,419,650 (GRCm39)	R671Q	probably benign	Het
Atp9a	A	G	2: 168,479,592 (GRCm39)	F928L	possibly damaging	Het
BC034090	A	T	1: 155,108,221 (GRCm39)	D13E	probably damaging	Het
Bco1	A	G	8: 117,855,980 (GRCm39)	E425G	probably benign	Het
Brsk1	T	C	7: 4,710,298 (GRCm39)	S436P	possibly damaging	Het
C1s1	T	C	6: 124,513,449 (GRCm39)	I193V	probably benign	Het
Calb2	A	G	8: 110,894,709 (GRCm39)	F21L	probably benign	Het
Catsperz	T	G	19: 6,902,171 (GRCm39)	T108P	probably benign	Het
Cep57l1	T	A	10: 41,595,767 (GRCm39)	D329V	possibly damaging	Het
Cfdp1	A	G	8: 112,557,577 (GRCm39)	F188S	probably benign	Het
Chrna2	G	A	14: 66,386,292 (GRCm39)	G146D	probably damaging	Het
Col6a1	A	C	10: 76,550,506 (GRCm39)	F520V	unknown	Het
Cyb5d2	C	A	11: 72,669,771 (GRCm39)	V43L	probably damaging	Het
Cyp2c40	T	C	19: 39,775,290 (GRCm39)	T321A	probably benign	Het
Dnajc16	A	C	4: 141,490,859 (GRCm39)	Y764D	probably damaging	Het
Dsg1a	G	A	18: 20,473,590 (GRCm39)	V888M	probably damaging	Het
F5	A	C	1: 164,012,489 (GRCm39)	T468P	probably damaging	Het
Faap24	A	G	7: 35,094,509 (GRCm39)	M97T	probably benign	Het
Fam227b	A	T	2: 125,849,230 (GRCm39)	I334N	probably damaging	Het
Frem2	G	T	3: 53,562,864 (GRCm39)	P548T	probably damaging	Het
Gfm1	A	G	3: 67,340,731 (GRCm39)	E94G	probably damaging	Het
Gm17606	A	T	14: 54,885,696 (GRCm39)		probably benign	Het
Gnb2	A	T	5: 137,528,515 (GRCm39)	M1K	probably null	Het
Gys1	G	A	7: 45,104,258 (GRCm39)	A544T	probably damaging	Het
Hdac5	T	C	11: 102,096,675 (GRCm39)	Y230C	probably damaging	Het
Hunk	A	T	16: 90,244,196 (GRCm39)		probably null	Het
Ifnl2	A	G	7: 28,209,635 (GRCm39)	F51L	probably damaging	Het
Itpr1	T	C	6: 108,387,892 (GRCm39)		probably null	Het
Kcnj1	A	G	9: 32,307,918 (GRCm39)	Y114C	probably benign	Het
Kdm4b	T	A	17: 56,706,459 (GRCm39)	S322T	probably damaging	Het
Kif27	T	C	13: 58,471,730 (GRCm39)	E786G	probably damaging	Het
Kif6	T	C	17: 50,060,909 (GRCm39)	V414A	probably benign	Het
L1td1	A	G	4: 98,621,861 (GRCm39)	K141R	possibly damaging	Het
Loxhd1	A	G	18: 77,490,581 (GRCm39)	I1394V	possibly damaging	Het
Lrfn5	A	T	12: 61,886,433 (GRCm39)	M74L	probably damaging	Het
Lrp6	C	T	6: 134,488,230 (GRCm39)	D289N	probably benign	Het
Mroh7	A	G	4: 106,548,710 (GRCm39)		probably null	Het
Myo18b	G	T	5: 113,023,041 (GRCm39)		probably benign	Het
Ncln	G	A	10: 81,328,902 (GRCm39)	A172V	probably damaging	Het
Nek9	G	A	12: 85,367,666 (GRCm39)	T335M	possibly damaging	Het
Notch2	A	G	3: 98,042,829 (GRCm39)	Y1398C	probably damaging	Het
Or10g6	A	T	9: 39,933,823 (GRCm39)	I45F	probably damaging	Het
Or2d4	T	A	7: 106,544,074 (GRCm39)	I45F	probably damaging	Het
Or5p52	A	T	7: 107,502,188 (GRCm39)	H88L	probably benign	Het
Pax2	G	A	19: 44,749,376 (GRCm39)	V40M	probably damaging	Het
Pde6c	A	G	19: 38,157,887 (GRCm39)	Y637C	probably damaging	Het
Plppr5	A	G	3: 117,414,618 (GRCm39)	I80V	probably damaging	Het
Pold1	G	T	7: 44,182,233 (GRCm39)	P1100T	probably damaging	Het
Prune2	T	C	19: 16,977,387 (GRCm39)	Y41H	probably damaging	Het
Rgl2	C	T	17: 34,152,200 (GRCm39)	A329V	possibly damaging	Het
Rilp	T	A	11: 75,402,250 (GRCm39)	Y250N	probably damaging	Het
Rilpl1	A	G	5: 124,652,751 (GRCm39)	V19A	probably benign	Het
Rtp3	T	C	9: 110,815,519 (GRCm39)		probably null	Het
Rufy4	A	G	1: 74,172,266 (GRCm39)	K246E	probably damaging	Het
Saraf	C	A	8: 34,635,616 (GRCm39)	A306E	probably damaging	Het
Slc26a8	A	T	17: 28,857,658 (GRCm39)	N828K	probably benign	Het
Src	C	T	2: 157,311,852 (GRCm39)	P527S	probably damaging	Het
Susd3	C	T	13: 49,384,778 (GRCm39)		probably null	Het
Syngap1	T	C	17: 27,185,880 (GRCm39)	L1270P	probably damaging	Het
Taf1c	G	A	8: 120,325,589 (GRCm39)	P758S	probably damaging	Het
Tenm2	A	T	11: 35,915,275 (GRCm39)	N2087K	probably damaging	Het
Tfrc	A	T	16: 32,448,969 (GRCm39)	I703F	probably damaging	Het
Thap1	C	G	8: 26,650,874 (GRCm39)	T48S	probably benign	Het
Tspear	T	C	10: 77,702,163 (GRCm39)	F199L	probably benign	Het
Usp17lc	A	T	7: 103,067,797 (GRCm39)	H364L	probably benign	Het
Usp9x	A	G	X: 12,989,747 (GRCm39)	R776G	possibly damaging	Homo
Vmn1r1	T	C	1: 181,984,789 (GRCm39)	E292G	possibly damaging	Het
Vmn1r125	T	G	7: 21,006,552 (GRCm39)	V150G	probably damaging	Het
Vmn1r167	A	T	7: 23,204,117 (GRCm39)	L300I	probably damaging	Het
Vmn2r106	A	C	17: 20,487,885 (GRCm39)	I838S	probably benign	Het
Wdr64	A	T	1: 175,554,060 (GRCm39)	S197C	probably damaging	Het
Zfp248	A	T	6: 118,410,268 (GRCm39)	V47E	possibly damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,599,186 (GRCm39)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,574,613 (GRCm39)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,569,728 (GRCm39)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,575,465 (GRCm39)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,140 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,139 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,114 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,576,179 (GRCm39)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,575,246 (GRCm39)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,575,362 (GRCm39)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,584,340 (GRCm39)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,569,473 (GRCm39)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,577,001 (GRCm39)	missense	unknown
IGL01625:Muc4	APN	16	32,575,918 (GRCm39)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,555,220 (GRCm39)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,581,722 (GRCm39)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,574,624 (GRCm39)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,570,244 (GRCm39)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,575,383 (GRCm39)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,598,023 (GRCm39)	splice site	probably benign
IGL02210:Muc4	APN	16	32,574,054 (GRCm39)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,569,668 (GRCm39)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,570,894 (GRCm39)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,576,503 (GRCm39)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,596,193 (GRCm39)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,575,656 (GRCm39)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,570,466 (GRCm39)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,569,212 (GRCm39)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,570,866 (GRCm39)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,570,766 (GRCm39)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,570,257 (GRCm39)	nonsense	probably null
IGL03335:Muc4	APN	16	32,574,449 (GRCm39)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,575,436 (GRCm39)	missense	probably benign	0.01
multisplendored	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,590,768 (GRCm39)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,580,625 (GRCm39)	splice site	probably benign
BB004:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
BB014:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
IGL02835:Muc4	UTSW	16	32,584,319 (GRCm39)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,580,622 (GRCm39)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,575,542 (GRCm39)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,576,391 (GRCm39)	missense	unknown
R0119:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,591,978 (GRCm39)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,586,120 (GRCm39)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,574,333 (GRCm39)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,582,910 (GRCm39)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,569,887 (GRCm39)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,577,120 (GRCm39)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,570,925 (GRCm39)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,570,488 (GRCm39)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,590,201 (GRCm39)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,590,221 (GRCm39)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,599,329 (GRCm39)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,576,570 (GRCm39)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,569,343 (GRCm39)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,574,448 (GRCm39)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,574,033 (GRCm39)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,569,167 (GRCm39)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,575,875 (GRCm39)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,577,276 (GRCm39)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,576,307 (GRCm39)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,576,625 (GRCm39)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,576,715 (GRCm39)	missense	unknown
R1928:Muc4	UTSW	16	32,569,350 (GRCm39)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,569,460 (GRCm39)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,570,121 (GRCm39)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,574,054 (GRCm39)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,576,783 (GRCm39)	missense	unknown
R2121:Muc4	UTSW	16	32,580,612 (GRCm39)	missense	unknown
R2139:Muc4	UTSW	16	32,581,599 (GRCm39)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,569,294 (GRCm39)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,587,316 (GRCm39)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,576,265 (GRCm39)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,577,846 (GRCm39)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,575,647 (GRCm39)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,576,200 (GRCm39)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,590,666 (GRCm39)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,577,075 (GRCm39)	missense	unknown
R4523:Muc4	UTSW	16	32,555,154 (GRCm39)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,576,217 (GRCm39)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,577,432 (GRCm39)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4701:Muc4	UTSW	16	32,576,220 (GRCm39)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,570,032 (GRCm39)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,596,277 (GRCm39)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,574,916 (GRCm39)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,570,565 (GRCm39)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,576,472 (GRCm39)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,575,437 (GRCm39)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,575,332 (GRCm39)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,576,670 (GRCm39)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,577,432 (GRCm39)	nonsense	probably null
R5161:Muc4	UTSW	16	32,582,895 (GRCm39)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,570,556 (GRCm39)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,570,484 (GRCm39)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,581,635 (GRCm39)	missense	unknown
R5567:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,569,600 (GRCm39)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,597,181 (GRCm39)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,555,059 (GRCm39)	nonsense	probably null
R5708:Muc4	UTSW	16	32,575,515 (GRCm39)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,570,734 (GRCm39)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,595,213 (GRCm39)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,570,113 (GRCm39)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,570,523 (GRCm39)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,574,480 (GRCm39)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,576,652 (GRCm39)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,579,682 (GRCm39)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,596,157 (GRCm39)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,577,169 (GRCm39)	missense	unknown
R6144:Muc4	UTSW	16	32,587,298 (GRCm39)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,554,958 (GRCm39)	start gained	probably benign
R6268:Muc4	UTSW	16	32,589,141 (GRCm39)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,570,853 (GRCm39)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,575,237 (GRCm39)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,575,476 (GRCm39)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,587,725 (GRCm39)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,555,061 (GRCm39)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,599,320 (GRCm39)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,570,607 (GRCm39)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,579,694 (GRCm39)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,602,378 (GRCm39)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,587,312 (GRCm39)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,596,177 (GRCm39)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,582,892 (GRCm39)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,570,817 (GRCm39)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,570,628 (GRCm39)	nonsense	probably null
R7033:Muc4	UTSW	16	32,576,698 (GRCm39)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,577,465 (GRCm39)	missense
R7123:Muc4	UTSW	16	32,569,509 (GRCm39)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,582,862 (GRCm39)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,576,835 (GRCm39)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,575,710 (GRCm39)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,575,740 (GRCm39)	missense	unknown
R7381:Muc4	UTSW	16	32,601,285 (GRCm39)	missense
R7411:Muc4	UTSW	16	32,570,140 (GRCm39)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,587,324 (GRCm39)	missense
R7539:Muc4	UTSW	16	32,576,770 (GRCm39)	missense
R7544:Muc4	UTSW	16	32,555,016 (GRCm39)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,586,076 (GRCm39)	missense
R7596:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,574,161 (GRCm39)	nonsense	probably null
R7639:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,580,479 (GRCm39)	missense
R7651:Muc4	UTSW	16	32,576,949 (GRCm39)	missense
R7688:Muc4	UTSW	16	32,570,278 (GRCm39)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,574,439 (GRCm39)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,589,108 (GRCm39)	missense
R7763:Muc4	UTSW	16	32,574,739 (GRCm39)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,576,568 (GRCm39)	missense	unknown
R7787:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,570,139 (GRCm39)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
R7930:Muc4	UTSW	16	32,575,369 (GRCm39)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,577,123 (GRCm39)	missense
R8096:Muc4	UTSW	16	32,575,764 (GRCm39)	missense	unknown
R8115:Muc4	UTSW	16	32,575,678 (GRCm39)	missense	unknown
R8162:Muc4	UTSW	16	32,569,197 (GRCm39)	missense	unknown
R8220:Muc4	UTSW	16	32,575,701 (GRCm39)	missense	unknown
R8260:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,575,434 (GRCm39)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,576,271 (GRCm39)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,574,201 (GRCm39)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,574,799 (GRCm39)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,574,421 (GRCm39)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,575,629 (GRCm39)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,602,356 (GRCm39)	missense
R8845:Muc4	UTSW	16	32,576,889 (GRCm39)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,569,461 (GRCm39)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,570,280 (GRCm39)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,575,285 (GRCm39)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,574,009 (GRCm39)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,582,910 (GRCm39)	missense
R9034:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,577,842 (GRCm39)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,589,102 (GRCm39)	missense
R9336:Muc4	UTSW	16	32,569,756 (GRCm39)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,569,971 (GRCm39)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,576,992 (GRCm39)	missense
R9429:Muc4	UTSW	16	32,576,098 (GRCm39)	missense	unknown
R9570:Muc4	UTSW	16	32,569,695 (GRCm39)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,577,234 (GRCm39)	missense
R9696:Muc4	UTSW	16	32,574,712 (GRCm39)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,590,644 (GRCm39)	missense
R9743:Muc4	UTSW	16	32,601,194 (GRCm39)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,570,676 (GRCm39)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,570,643 (GRCm39)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,575,855 (GRCm39)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,579,693 (GRCm39)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,576,450 (GRCm39)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,587,767 (GRCm39)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,595,236 (GRCm39)	missense
Z1177:Muc4	UTSW	16	32,587,768 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ATTGAGGCATGGTCAGGATAC -3'
(R):5'- TGCCAAGCAGGAAATTAGCC -3'

Sequencing Primer
(F):5'- AGTCACTGCTGCCGAGTCTAAG -3'
(R):5'- TTAGCCTAAACAGTGTGTGAAGC -3'

Posted On

2015-10-08