Incidental Mutation 'R4685:Try5'
ID353598
Institutional Source Beutler Lab
Gene Symbol Try5
Ensembl Gene ENSMUSG00000036938
Gene Nametrypsin 5
Synonyms1810049H19Rik, Tc
MMRRC Submission 041936-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.075) question?
Stock #R4685 (G1)
Quality Score225
Status Validated
Chromosome6
Chromosomal Location41311233-41316841 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 41311299 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Leucine at position 240 (Q240L)
Ref Sequence ENSEMBL: ENSMUSP00000064498 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000064324] [ENSMUST00000173916]
Predicted Effect possibly damaging
Transcript: ENSMUST00000064324
AA Change: Q240L

PolyPhen 2 Score 0.593 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000064498
Gene: ENSMUSG00000036938
AA Change: Q240L

DomainStartEndE-ValueType
low complexity region 4 16 N/A INTRINSIC
Tryp_SPc 23 239 1.47e-104 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000173916
AA Change: Q132L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000133640
Gene: ENSMUSG00000036938
AA Change: Q132L

DomainStartEndE-ValueType
Tryp_SPc 1 131 1.21e-27 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000193013
Meta Mutation Damage Score 0.0932 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.1%
  • 20x: 95.0%
Validation Efficiency 97% (71/73)
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2410004P03Rik T C 12: 17,007,184 D104G probably damaging Het
2810021J22Rik T C 11: 58,880,924 S411P probably damaging Het
Adcy2 T C 13: 68,727,905 R493G probably benign Het
Adcy8 A G 15: 64,737,438 I874T probably benign Het
Ano2 G T 6: 125,980,124 E619* probably null Het
Apob A G 12: 8,006,456 K1613R probably benign Het
Arhgef10 T A 8: 14,956,963 F476Y probably damaging Het
Bmf G A 2: 118,546,802 A74V probably damaging Het
Cadps T C 14: 12,467,139 E925G possibly damaging Het
Ccdc162 T C 10: 41,681,686 D181G possibly damaging Het
Ccdc85c A T 12: 108,207,175 C387S probably benign Het
Cntnap5a T C 1: 116,446,680 V974A possibly damaging Het
Dnah7b T C 1: 46,211,328 F1703S probably damaging Het
Dsg3 C A 18: 20,539,736 D821E probably benign Het
Ecel1 G T 1: 87,152,946 probably null Het
Edn1 A T 13: 42,305,253 probably null Het
Egfr G A 11: 16,858,980 C58Y probably damaging Het
Fam184a T C 10: 53,698,500 N282D probably benign Het
Fhit T A 14: 9,870,091 Q63L probably damaging Het
Gabarapl2 T C 8: 111,942,518 V36A probably benign Het
Glis1 T C 4: 107,567,645 V151A probably benign Het
Gm12695 A G 4: 96,761,980 S210P probably damaging Het
Gpat4 TAGAAGA TAGA 8: 23,182,849 probably benign Het
H2-M10.4 G A 17: 36,461,796 A98V probably benign Het
Hhat C A 1: 192,595,054 G366C probably damaging Het
Hydin C A 8: 110,462,522 A1186E probably damaging Het
Itgb2 T A 10: 77,550,103 probably null Het
Kank1 C T 19: 25,410,034 A329V possibly damaging Het
Kdm4b T A 17: 56,401,675 S1070T probably benign Het
Kyat1 A G 2: 30,188,265 Y101H probably damaging Het
Lman1l T A 9: 57,609,200 I407F probably damaging Het
Map4k5 T C 12: 69,811,366 K679R probably benign Het
Mill1 A T 7: 18,255,928 D45V probably damaging Het
Myo3a T A 2: 22,407,422 Y743N probably damaging Het
Nox4 C G 7: 87,297,508 I137M probably benign Het
Odf4 A G 11: 68,922,839 probably null Het
Ostf1 A T 19: 18,581,288 D210E probably damaging Het
Paxip1 A T 5: 27,761,677 probably null Het
Pitrm1 A G 13: 6,556,542 T211A probably benign Het
Pla2g4f A G 2: 120,305,015 S393P probably damaging Het
Plppr3 T C 10: 79,867,525 T42A probably damaging Het
Plxna4 C A 6: 32,165,844 G1559W probably damaging Het
Ppp1r13l T C 7: 19,375,383 probably null Het
Prex1 C T 2: 166,638,332 V163M probably damaging Het
Prl6a1 A G 13: 27,316,324 T93A probably benign Het
Psg16 T C 7: 17,090,534 V81A probably benign Het
Rbm33 A T 5: 28,408,282 probably benign Het
Rest C T 5: 77,275,243 P298L possibly damaging Het
Rhobtb3 G A 13: 75,878,932 R441* probably null Het
Rims4 T A 2: 163,864,994 K155* probably null Het
Rps6kb1 C T 11: 86,519,887 probably null Het
Ryr2 T C 13: 11,692,646 D2835G probably damaging Het
Sc5d C T 9: 42,258,650 V92I probably benign Het
Sell A T 1: 164,066,260 I175F probably damaging Het
Serpinb6d A G 13: 33,671,228 D295G probably damaging Het
Sphk1 A G 11: 116,535,280 D96G probably damaging Het
Spns3 A T 11: 72,537,270 V228D probably damaging Het
Sspo A G 6: 48,492,894 S4500G probably damaging Het
Syt9 T A 7: 107,436,471 C232S possibly damaging Het
Terf1 A G 1: 15,818,961 I176V possibly damaging Het
Tln2 C T 9: 67,302,572 A428T probably damaging Het
Tmprss7 T C 16: 45,679,348 N321S probably benign Het
Tomm40 A G 7: 19,701,836 I323T probably benign Het
Vmn1r213 A T 13: 23,011,630 I128L probably benign Het
Vmn2r80 T C 10: 79,194,328 F663L possibly damaging Het
Znfx1 T A 2: 167,039,030 Y278F probably damaging Het
Zpbp2 A G 11: 98,551,291 probably benign Het
Other mutations in Try5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01837:Try5 APN 6 41313424 missense probably benign 0.31
IGL01885:Try5 APN 6 41311738 missense possibly damaging 0.93
IGL02652:Try5 APN 6 41311408 missense probably benign 0.01
R1955:Try5 UTSW 6 41311769 missense probably benign 0.17
R2014:Try5 UTSW 6 41314651 splice site probably null
R2015:Try5 UTSW 6 41314651 splice site probably null
R2848:Try5 UTSW 6 41313476 missense probably benign 0.01
R4227:Try5 UTSW 6 41313467 missense possibly damaging 0.65
R4816:Try5 UTSW 6 41313415 missense probably benign 0.18
R5230:Try5 UTSW 6 41312378 missense probably benign 0.19
R5658:Try5 UTSW 6 41312427 missense probably damaging 1.00
R6518:Try5 UTSW 6 41314679 missense probably benign
R6910:Try5 UTSW 6 41311799 missense possibly damaging 0.62
R6913:Try5 UTSW 6 41311332 missense probably damaging 1.00
R7219:Try5 UTSW 6 41311703 missense probably damaging 1.00
R7242:Try5 UTSW 6 41313454 missense probably benign 0.09
R7444:Try5 UTSW 6 41311365 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- CTGCTGCTGAAATATTATTCCATGC -3'
(R):5'- CAGGTCAGAGTAATGTGGTCCC -3'

Sequencing Primer
(F):5'- CCATGCAACATATTTGGAGATGTAGG -3'
(R):5'- AGAGTAATGTGGTCCCTTTTCC -3'
Posted On2015-10-21