Mutagenetix > Incidental Mutation

Incidental Mutation 'R4689:Wdfy4'

354789

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

041940-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4689 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32681504-32907465 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32831505 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 907 (I907T)

Ref Sequence

ENSEMBL: ENSMUSP00000117068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000061753
AA Change: I907T

PolyPhen 2 Score 0.438 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: I907T

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130509
AA Change: I907T

PolyPhen 2 Score 0.884 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: I907T

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Meta Mutation Damage Score

0.1936

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

97% (72/74)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	C	A	17: 46,634,996 (GRCm39)	V336L	probably benign	Het
Acbd4	T	C	11: 102,996,194 (GRCm39)	L165P	possibly damaging	Het
Adam10	T	C	9: 70,673,236 (GRCm39)	S456P	possibly damaging	Het
Adgre4	T	C	17: 56,109,096 (GRCm39)	F368L	probably damaging	Het
Ahcyl1	G	T	3: 107,572,834 (GRCm39)	Y528*	probably null	Het
Aldh3b3	G	A	19: 4,014,516 (GRCm39)	V84M	probably damaging	Het
Cdca8	C	T	4: 124,824,896 (GRCm39)	G78E	probably damaging	Het
Cry2	T	C	2: 92,254,899 (GRCm39)	D152G	probably benign	Het
Cyp2c67	T	C	19: 39,627,032 (GRCm39)	Y266C	probably benign	Het
Dkk4	T	C	8: 23,115,336 (GRCm39)	F62S	probably benign	Het
Dnah12	G	A	14: 26,427,994 (GRCm39)	V207I	probably benign	Het
Dthd1	T	C	5: 63,000,255 (GRCm39)	C526R	probably damaging	Het
Dubr	A	T	16: 50,552,866 (GRCm39)		noncoding transcript	Het
F5	T	C	1: 163,979,542 (GRCm39)		probably benign	Het
Flcn	A	C	11: 59,691,870 (GRCm39)	W260G	possibly damaging	Het
Fmnl1	G	A	11: 103,084,562 (GRCm39)		probably null	Het
Frem2	A	T	3: 53,455,056 (GRCm39)	D2173E	probably benign	Het
Fstl4	C	T	11: 52,959,477 (GRCm39)	Q173*	probably null	Het
Gfra3	G	A	18: 34,823,640 (GRCm39)	P381S	unknown	Het
Gm28308	C	A	6: 52,190,291 (GRCm39)		probably benign	Het
Gm8730	T	A	8: 103,592,379 (GRCm39)		noncoding transcript	Het
Gzmd	T	C	14: 56,368,683 (GRCm39)		probably null	Het
Hexb	A	G	13: 97,317,600 (GRCm39)	Y366H	probably damaging	Het
Hydin	A	T	8: 111,322,046 (GRCm39)	H4566L	probably benign	Het
Ifi213	G	A	1: 173,417,986 (GRCm39)	T142I	possibly damaging	Het
Kif13b	T	A	14: 65,010,513 (GRCm39)	C1271S	probably damaging	Het
Krtap9-5	G	T	11: 99,840,286 (GRCm39)	C329F	unknown	Het
Larp1	G	T	11: 57,932,439 (GRCm39)	G207W	probably damaging	Het
Lfng	A	G	5: 140,600,194 (GRCm39)	D368G	probably damaging	Het
Mbd5	G	A	2: 49,148,291 (GRCm39)	V834I	possibly damaging	Het
Mterf1b	T	A	5: 4,247,263 (GRCm39)	Y301*	probably null	Het
Myh7b	T	C	2: 155,472,434 (GRCm39)	I1305T	possibly damaging	Het
Myo16	T	C	8: 10,488,890 (GRCm39)	V687A	probably damaging	Het
Naip2	A	T	13: 100,285,320 (GRCm39)	I1292N	probably damaging	Het
Nmral1	A	G	16: 4,532,422 (GRCm39)	F130L	probably damaging	Het
Nos1	A	G	5: 118,017,450 (GRCm39)	N271S	probably benign	Het
Nrap	A	G	19: 56,374,458 (GRCm39)	S23P	probably damaging	Het
Or12d16-ps1	A	T	17: 37,705,662 (GRCm39)	N77I	probably damaging	Het
Or2aj4	A	T	16: 19,385,263 (GRCm39)	Y123*	probably null	Het
Or4e2	T	C	14: 52,688,671 (GRCm39)	I267T	probably benign	Het
Or6c210	T	C	10: 129,496,185 (GRCm39)	V170A	probably benign	Het
Pkdcc	C	G	17: 83,523,290 (GRCm39)	C132W	probably damaging	Het
Plekhg6	G	A	6: 125,350,144 (GRCm39)	L265F	probably benign	Het
Prkaa1	A	G	15: 5,208,177 (GRCm39)	T473A	probably benign	Het
Prpf3	G	A	3: 95,743,801 (GRCm39)	Q451*	probably null	Het
Psma5-ps	T	A	10: 85,150,065 (GRCm39)		noncoding transcript	Het
Ptprh	T	A	7: 4,600,996 (GRCm39)	D127V	possibly damaging	Het
Rab36	T	C	10: 74,877,765 (GRCm39)		probably null	Het
Rasa1	A	T	13: 85,386,282 (GRCm39)	Y427*	probably null	Het
Rgs11	T	C	17: 26,423,521 (GRCm39)		probably null	Het
Serpinb13	C	T	1: 106,910,574 (GRCm39)	S66L	probably damaging	Het
Shank2	G	A	7: 143,974,342 (GRCm39)	V1087I	probably benign	Het
Slc39a10	A	C	1: 46,875,173 (GRCm39)	M43R	probably benign	Het
Slc40a1	T	A	1: 45,951,473 (GRCm39)	Q228L	probably benign	Het
Slc45a1	A	T	4: 150,722,996 (GRCm39)	L296Q	probably benign	Het
Stambpl1	A	G	19: 34,213,691 (GRCm39)	T307A	probably benign	Het
Stt3a	T	C	9: 36,644,225 (GRCm39)	T705A	possibly damaging	Het
Tec	G	A	5: 72,980,980 (GRCm39)		probably benign	Het
Trp63	A	T	16: 25,684,012 (GRCm39)	T300S	possibly damaging	Het
Vmn1r235	A	G	17: 21,482,623 (GRCm39)	H316R	probably benign	Het
Vmn2r1	A	G	3: 64,012,074 (GRCm39)	H645R	possibly damaging	Het
Zcchc10	A	G	11: 53,218,151 (GRCm39)	T33A	probably benign	Het
Zfp318	T	A	17: 46,710,560 (GRCm39)	V761D	probably damaging	Het
Zfp358	T	C	8: 3,546,146 (GRCm39)		probably null	Het
Zfp661	G	A	2: 127,419,468 (GRCm39)	P224L	probably damaging	Het
Zfp937	T	C	2: 150,078,706 (GRCm39)	M33T	probably damaging	Het
Zfp955a	T	C	17: 33,461,040 (GRCm39)	H364R	probably damaging	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	32,824,496 (GRCm39)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,681,934 (GRCm39)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	32,825,994 (GRCm39)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	32,873,618 (GRCm39)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	32,742,195 (GRCm39)	splice site	probably benign
IGL01931:Wdfy4	APN	14	32,877,710 (GRCm39)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	32,855,673 (GRCm39)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	32,798,437 (GRCm39)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	32,815,257 (GRCm39)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	32,871,523 (GRCm39)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,688,389 (GRCm39)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	32,764,448 (GRCm39)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	32,812,818 (GRCm39)	nonsense	probably null
IGL02752:Wdfy4	APN	14	32,798,283 (GRCm39)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	32,817,262 (GRCm39)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,693,707 (GRCm39)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	32,831,607 (GRCm39)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	32,798,241 (GRCm39)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	32,862,608 (GRCm39)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,688,392 (GRCm39)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	32,884,827 (GRCm39)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	32,847,844 (GRCm39)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,699,124 (GRCm39)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	32,790,861 (GRCm39)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	32,769,247 (GRCm39)	missense	probably benign	0.14
dodgers	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
Dollar	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
Giants	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
gigantea	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
kings_canyon	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
moro	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
popped	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
sequoia	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
Sherman	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
stretched	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
watchtower	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	32,829,130 (GRCm39)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	32,884,708 (GRCm39)	missense	probably null	1.00
R0085:Wdfy4	UTSW	14	32,800,200 (GRCm39)	missense	possibly damaging	0.81
R0277:Wdfy4	UTSW	14	32,805,742 (GRCm39)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	32,805,769 (GRCm39)	splice site	probably benign
R0496:Wdfy4	UTSW	14	32,862,695 (GRCm39)	splice site	probably benign
R0514:Wdfy4	UTSW	14	32,802,732 (GRCm39)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	32,764,578 (GRCm39)	missense	probably benign
R0590:Wdfy4	UTSW	14	32,763,131 (GRCm39)	missense	probably benign	0.09
R0647:Wdfy4	UTSW	14	32,831,656 (GRCm39)	missense	possibly damaging	0.96
R0766:Wdfy4	UTSW	14	32,862,569 (GRCm39)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	32,801,923 (GRCm39)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,693,695 (GRCm39)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,693,729 (GRCm39)	splice site	probably null
R1415:Wdfy4	UTSW	14	32,763,137 (GRCm39)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	32,830,645 (GRCm39)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	32,822,923 (GRCm39)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	32,874,495 (GRCm39)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,682,765 (GRCm39)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,681,918 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	32,795,542 (GRCm39)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	32,817,962 (GRCm39)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	32,825,940 (GRCm39)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	32,855,301 (GRCm39)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,693,641 (GRCm39)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	32,828,001 (GRCm39)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	32,868,946 (GRCm39)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	32,795,468 (GRCm39)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	32,884,764 (GRCm39)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R2889:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R3114:Wdfy4	UTSW	14	32,811,860 (GRCm39)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	32,862,602 (GRCm39)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,688,352 (GRCm39)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	32,824,637 (GRCm39)	splice site	probably benign
R4091:Wdfy4	UTSW	14	32,847,837 (GRCm39)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	32,818,040 (GRCm39)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	32,809,912 (GRCm39)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4629:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,711,893 (GRCm39)	missense	probably damaging	0.98
R4718:Wdfy4	UTSW	14	32,867,273 (GRCm39)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,710,852 (GRCm39)	nonsense	probably null
R4894:Wdfy4	UTSW	14	32,877,717 (GRCm39)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	32,769,213 (GRCm39)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	32,750,970 (GRCm39)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	32,822,897 (GRCm39)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	32,801,892 (GRCm39)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	32,874,627 (GRCm39)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	32,769,232 (GRCm39)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	32,800,300 (GRCm39)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	32,812,823 (GRCm39)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,710,856 (GRCm39)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,681,959 (GRCm39)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	32,873,689 (GRCm39)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	32,829,087 (GRCm39)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	32,855,454 (GRCm39)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
R5890:Wdfy4	UTSW	14	32,824,534 (GRCm39)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	32,855,317 (GRCm39)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	32,827,968 (GRCm39)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,693,668 (GRCm39)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	32,831,482 (GRCm39)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	32,823,006 (GRCm39)	nonsense	probably null
R6370:Wdfy4	UTSW	14	32,790,807 (GRCm39)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	32,826,051 (GRCm39)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,689,604 (GRCm39)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	32,830,649 (GRCm39)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	32,868,901 (GRCm39)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	32,769,208 (GRCm39)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,681,923 (GRCm39)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	32,821,074 (GRCm39)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,682,777 (GRCm39)	missense
R7114:Wdfy4	UTSW	14	32,693,531 (GRCm39)	splice site	probably null
R7139:Wdfy4	UTSW	14	32,873,535 (GRCm39)	missense
R7255:Wdfy4	UTSW	14	32,696,239 (GRCm39)	missense
R7324:Wdfy4	UTSW	14	32,769,271 (GRCm39)	missense
R7379:Wdfy4	UTSW	14	32,873,566 (GRCm39)	missense
R7399:Wdfy4	UTSW	14	32,790,863 (GRCm39)	missense
R7408:Wdfy4	UTSW	14	32,800,264 (GRCm39)	missense
R7410:Wdfy4	UTSW	14	32,696,191 (GRCm39)	missense
R7411:Wdfy4	UTSW	14	32,828,088 (GRCm39)	missense
R7412:Wdfy4	UTSW	14	32,871,541 (GRCm39)	missense
R7445:Wdfy4	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,707,696 (GRCm39)	missense
R7622:Wdfy4	UTSW	14	32,800,231 (GRCm39)	missense
R7828:Wdfy4	UTSW	14	32,710,878 (GRCm39)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	32,812,920 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,792,705 (GRCm39)	missense
R7986:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7990:Wdfy4	UTSW	14	32,819,752 (GRCm39)	missense
R8001:Wdfy4	UTSW	14	32,695,492 (GRCm39)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,693,584 (GRCm39)	missense
R8015:Wdfy4	UTSW	14	32,829,704 (GRCm39)	missense
R8032:Wdfy4	UTSW	14	32,751,043 (GRCm39)	nonsense	probably null
R8041:Wdfy4	UTSW	14	32,875,965 (GRCm39)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8092:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8112:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8114:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8115:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
R8118:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8140:Wdfy4	UTSW	14	32,864,317 (GRCm39)	missense
R8155:Wdfy4	UTSW	14	32,884,776 (GRCm39)	missense
R8163:Wdfy4	UTSW	14	32,873,545 (GRCm39)	missense
R8293:Wdfy4	UTSW	14	32,696,218 (GRCm39)	missense
R8325:Wdfy4	UTSW	14	32,689,444 (GRCm39)	missense
R8353:Wdfy4	UTSW	14	32,695,581 (GRCm39)	missense	probably benign
R8370:Wdfy4	UTSW	14	32,815,208 (GRCm39)	missense
R8437:Wdfy4	UTSW	14	32,798,332 (GRCm39)	missense
R8497:Wdfy4	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	32,800,258 (GRCm39)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,693,722 (GRCm39)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,689,489 (GRCm39)	missense
R8747:Wdfy4	UTSW	14	32,874,611 (GRCm39)	missense
R8794:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	32,867,105 (GRCm39)	missense
R8880:Wdfy4	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
R9109:Wdfy4	UTSW	14	32,760,704 (GRCm39)	splice site	probably null
R9131:Wdfy4	UTSW	14	32,819,807 (GRCm39)	missense
R9309:Wdfy4	UTSW	14	32,817,313 (GRCm39)	missense
R9349:Wdfy4	UTSW	14	32,875,996 (GRCm39)	missense
R9451:Wdfy4	UTSW	14	32,855,518 (GRCm39)	missense
R9563:Wdfy4	UTSW	14	32,692,833 (GRCm39)	missense
R9587:Wdfy4	UTSW	14	32,769,230 (GRCm39)	nonsense	probably null
R9599:Wdfy4	UTSW	14	32,855,428 (GRCm39)	missense
R9670:Wdfy4	UTSW	14	32,769,219 (GRCm39)	missense
R9718:Wdfy4	UTSW	14	32,847,893 (GRCm39)	missense
R9742:Wdfy4	UTSW	14	32,809,987 (GRCm39)	missense
X0028:Wdfy4	UTSW	14	32,802,593 (GRCm39)	missense	probably benign
X0053:Wdfy4	UTSW	14	32,884,899 (GRCm39)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
Z1177:Wdfy4	UTSW	14	32,809,942 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACGAGCCTATGTCTGTTCTGTG -3'
(R):5'- CCAGACTTACAGCTTGCTTTG -3'

Sequencing Primer
(F):5'- ATGTCTGTTCTGTGTACATATGCATC -3'
(R):5'- AGACTTACAGCTTGCTTTGATTGC -3'

Posted On

2015-10-21