Mutagenetix > Incidental Mutation

Incidental Mutation 'R4702:Papln'

356156

Institutional Source

Beutler Lab

Gene Symbol

Papln

Ensembl Gene

ENSMUSG00000021223

Gene Name

papilin, proteoglycan-like sulfated glycoprotein

Synonyms

E030033C16Rik

MMRRC Submission

041950-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4702 (G1)

Quality Score

190

Status

Not validated

Chromosome

Chromosomal Location

83810408-83839156 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 83828757 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 821 (T821I)

Ref Sequence

ENSEMBL: ENSMUSP00000113806 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021646] [ENSMUST00000121733]

AlphaFold

Q9EPX2

Predicted Effect

probably benign
Transcript: ENSMUST00000021646
AA Change: T799I

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000021646
Gene: ENSMUSG00000021223
AA Change: T799I

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TSP1	30	81	3.36e-11	SMART
low complexity region	147	161	N/A	INTRINSIC
Pfam:ADAM_spacer1	184	299	3.3e-39	PFAM
TSP1	309	362	1.2e-7	SMART
TSP1	366	426	2.76e-7	SMART
TSP1	427	482	1.42e-9	SMART
TSP1	488	540	2.47e-9	SMART
low complexity region	604	621	N/A	INTRINSIC
KU	748	801	1.83e-22	SMART
low complexity region	822	831	N/A	INTRINSIC
IGc2	917	980	2.88e-4	SMART
IGc2	1056	1119	2.66e-17	SMART
IGc2	1145	1209	2.13e-7	SMART
Pfam:PLAC	1234	1268	2.3e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000121733
AA Change: T821I

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000113806
Gene: ENSMUSG00000021223
AA Change: T821I

Domain	Start	End	E-Value	Type
low complexity region	3	16	N/A	INTRINSIC
TSP1	30	81	3.36e-11	SMART
low complexity region	147	161	N/A	INTRINSIC
Pfam:ADAM_spacer1	184	299	2.8e-38	PFAM
TSP1	309	362	1.2e-7	SMART
TSP1	388	448	1.82e-7	SMART
TSP1	449	504	1.42e-9	SMART
TSP1	510	562	2.47e-9	SMART
low complexity region	626	643	N/A	INTRINSIC
KU	770	823	1.83e-22	SMART
Pfam:Papilin_u7	831	922	1.9e-40	PFAM
IGc2	939	1002	2.88e-4	SMART
IGc2	1078	1141	2.66e-17	SMART
IGc2	1167	1231	2.13e-7	SMART
Pfam:PLAC	1257	1289	1.1e-13	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152904

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	T	C	2: 151,314,509 (GRCm39)	T390A	probably benign	Het
Adam25	G	T	8: 41,207,163 (GRCm39)	C143F	probably damaging	Het
Adra1a	T	C	14: 66,875,008 (GRCm39)		probably benign	Het
Aff1	T	C	5: 103,958,935 (GRCm39)	Y343H	probably damaging	Het
Antxr2	C	T	5: 98,097,028 (GRCm39)		probably null	Het
Ap1m2	A	G	9: 21,209,591 (GRCm39)	F362L	probably benign	Het
Apobec4	A	G	1: 152,632,001 (GRCm39)	T10A	probably benign	Het
Armc3	A	G	2: 19,314,792 (GRCm39)	N822S	probably damaging	Het
Atr	A	C	9: 95,802,408 (GRCm39)	T1767P	possibly damaging	Het
B4galnt1	G	T	10: 127,003,394 (GRCm39)	V172F	possibly damaging	Het
Bloc1s1	T	A	10: 128,759,267 (GRCm39)	Q13L	probably damaging	Het
Blvra	A	C	2: 126,933,982 (GRCm39)	I125L	probably benign	Het
Caps2	T	C	10: 112,044,252 (GRCm39)	F484L	probably damaging	Het
Cep76	A	T	18: 67,767,968 (GRCm39)	I188K	possibly damaging	Het
Cidea	T	A	18: 67,500,498 (GRCm39)	F187I	probably benign	Het
Cntn3	T	A	6: 102,142,292 (GRCm39)	N1025I	probably benign	Het
Cntnap3	A	T	13: 64,926,676 (GRCm39)	C565S	probably benign	Het
Cyp2d22	A	C	15: 82,260,118 (GRCm39)	L22R	probably damaging	Het
Cyp2d26	C	T	15: 82,676,648 (GRCm39)		probably benign	Het
Cyp2j12	A	T	4: 96,021,230 (GRCm39)		probably null	Het
Dnajb13	T	C	7: 100,153,748 (GRCm39)	N229S	probably benign	Het
Dpys	A	G	15: 39,656,798 (GRCm39)	V423A	possibly damaging	Het
Eif4e1b	A	T	13: 54,935,138 (GRCm39)	I222F	probably damaging	Het
Eif5b	A	T	1: 38,057,958 (GRCm39)	N87Y	unknown	Het
Enam	T	A	5: 88,651,650 (GRCm39)	L1053*	probably null	Het
Epha7	T	A	4: 28,961,425 (GRCm39)	L890Q	probably damaging	Het
Fancm	T	A	12: 65,168,826 (GRCm39)	S1730T	possibly damaging	Het
Flrt3	A	G	2: 140,503,575 (GRCm39)	F18L	probably benign	Het
Git2	A	G	5: 114,883,543 (GRCm39)	S396P	probably damaging	Het
H2-M10.4	A	G	17: 36,772,874 (GRCm39)	I36T	probably benign	Het
Igfn1	G	A	1: 135,894,947 (GRCm39)	S1873L	possibly damaging	Het
Ints12	A	T	3: 132,802,546 (GRCm39)	D10V	probably damaging	Het
Kbtbd2	A	G	6: 56,756,288 (GRCm39)	S483P	probably benign	Het
Kcnv2	G	C	19: 27,300,967 (GRCm39)	A273P	probably damaging	Het
Klc3	T	G	7: 19,129,756 (GRCm39)	D371A	probably damaging	Het
Lama3	T	A	18: 12,711,086 (GRCm39)	L1567*	probably null	Het
Ldhal6b	G	T	17: 5,468,582 (GRCm39)	H117Q	probably damaging	Het
Lrriq1	G	T	10: 103,051,610 (GRCm39)	Q381K	possibly damaging	Het
Mrps31	T	A	8: 22,909,754 (GRCm39)	L140Q	probably damaging	Het
Myo15b	A	G	11: 115,774,834 (GRCm39)	T2119A	probably benign	Het
Nkx6-2	T	C	7: 139,161,456 (GRCm39)	D243G	probably damaging	Het
Or1ab2	T	A	8: 72,864,044 (GRCm39)	F211L	probably damaging	Het
Or8b3b	A	T	9: 38,584,776 (GRCm39)	M1K	probably null	Het
Pitpnb	T	G	5: 111,519,218 (GRCm39)	V166G	probably benign	Het
Pla2g15	T	A	8: 106,889,691 (GRCm39)	M321K	probably benign	Het
Plcxd3	C	A	15: 4,405,269 (GRCm39)	S25R	probably benign	Het
Ppargc1a	T	C	5: 51,653,038 (GRCm39)	I175V	possibly damaging	Het
Ppp1r13b	T	A	12: 111,799,715 (GRCm39)	Q687H	probably benign	Het
Prpf3	A	G	3: 95,741,404 (GRCm39)	V584A	probably damaging	Het
Psen2	A	G	1: 180,055,289 (GRCm39)	L399S	probably damaging	Het
Ptchd3	G	A	11: 121,727,235 (GRCm39)	V370I	probably damaging	Het
Rasa3	G	T	8: 13,620,394 (GRCm39)	D758E	probably benign	Het
Reck	T	C	4: 43,898,060 (GRCm39)	C113R	probably damaging	Het
Resf1	A	G	6: 149,230,901 (GRCm39)	T1316A	probably benign	Het
Ric1	T	A	19: 29,575,417 (GRCm39)	F1009I	possibly damaging	Het
Rrp12	T	C	19: 41,859,975 (GRCm39)	N1035S	probably damaging	Het
Rtel1	T	A	2: 180,993,962 (GRCm39)	S849T	probably benign	Het
Scn10a	A	T	9: 119,462,857 (GRCm39)	Y1060N	possibly damaging	Het
Selenov	A	G	7: 27,987,436 (GRCm39)	L314P	probably damaging	Het
Selplg	T	C	5: 113,957,094 (GRCm39)	D404G	probably benign	Het
Slc7a14	T	A	3: 31,284,547 (GRCm39)	Y263F	probably damaging	Het
Slco3a1	A	G	7: 73,970,315 (GRCm39)	S431P	probably damaging	Het
Speer3	C	G	5: 13,846,394 (GRCm39)	A238G	possibly damaging	Het
Spopl	A	T	2: 23,405,309 (GRCm39)		probably null	Het
Stk10	A	T	11: 32,505,172 (GRCm39)	T69S	probably benign	Het
Tbxas1	T	C	6: 39,060,791 (GRCm39)		probably null	Het
Tec	G	A	5: 72,941,074 (GRCm39)	P161L	possibly damaging	Het
Tnip1	A	G	11: 54,815,228 (GRCm39)	S339P	probably benign	Het
Tsen34	T	A	7: 3,703,632 (GRCm39)	V290D	probably damaging	Het
Tuba1a	T	A	15: 98,849,563 (GRCm39)	I5F	possibly damaging	Het
Vmn1r31	C	A	6: 58,448,953 (GRCm39)	*304L	probably null	Het
Vmn1r63	T	C	7: 5,806,516 (GRCm39)	R39G	possibly damaging	Het
Xylt2	A	G	11: 94,560,355 (GRCm39)	Y307H	possibly damaging	Het
Zfp65	A	G	13: 67,872,341 (GRCm39)	M1T	probably null	Het
Zmym4	T	C	4: 126,816,958 (GRCm39)	I247V	probably benign	Het

Other mutations in Papln

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00824:Papln	APN	12	83,817,210 (GRCm39)	missense	possibly damaging	0.81
IGL01788:Papln	APN	12	83,822,236 (GRCm39)	missense	probably benign	0.32
IGL01889:Papln	APN	12	83,833,609 (GRCm39)	missense	probably benign	0.25
IGL02499:Papln	APN	12	83,827,445 (GRCm39)	missense	probably benign	0.00
IGL02567:Papln	APN	12	83,825,611 (GRCm39)	missense	probably benign	0.00
IGL03150:Papln	APN	12	83,829,758 (GRCm39)	missense	probably damaging	1.00
IGL03331:Papln	APN	12	83,830,435 (GRCm39)	missense	probably benign
F5770:Papln	UTSW	12	83,825,608 (GRCm39)	missense	possibly damaging	0.72
R0201:Papln	UTSW	12	83,829,801 (GRCm39)	splice site	probably benign
R0389:Papln	UTSW	12	83,830,153 (GRCm39)	nonsense	probably null
R0763:Papln	UTSW	12	83,838,639 (GRCm39)	missense	possibly damaging	0.54
R1508:Papln	UTSW	12	83,829,690 (GRCm39)	missense	probably damaging	0.99
R1628:Papln	UTSW	12	83,831,180 (GRCm39)	splice site	probably benign
R1920:Papln	UTSW	12	83,836,028 (GRCm39)	nonsense	probably null
R1974:Papln	UTSW	12	83,828,811 (GRCm39)	missense	probably damaging	0.98
R2004:Papln	UTSW	12	83,819,992 (GRCm39)	missense	probably damaging	1.00
R2105:Papln	UTSW	12	83,827,010 (GRCm39)	missense	probably benign	0.04
R2876:Papln	UTSW	12	83,825,701 (GRCm39)	missense	probably damaging	0.96
R4199:Papln	UTSW	12	83,830,166 (GRCm39)	missense	probably null	0.01
R4705:Papln	UTSW	12	83,823,982 (GRCm39)	splice site	probably null
R4835:Papln	UTSW	12	83,821,194 (GRCm39)	missense	probably damaging	0.99
R4874:Papln	UTSW	12	83,823,917 (GRCm39)	missense	probably benign	0.01
R4938:Papln	UTSW	12	83,829,677 (GRCm39)	missense	probably benign	0.35
R5000:Papln	UTSW	12	83,821,663 (GRCm39)	missense	probably damaging	1.00
R5149:Papln	UTSW	12	83,818,656 (GRCm39)	splice site	probably null
R5324:Papln	UTSW	12	83,821,345 (GRCm39)	missense	probably damaging	1.00
R5784:Papln	UTSW	12	83,828,754 (GRCm39)	missense	probably benign
R5881:Papln	UTSW	12	83,818,652 (GRCm39)	missense	probably null	0.91
R5977:Papln	UTSW	12	83,831,143 (GRCm39)	nonsense	probably null
R6035:Papln	UTSW	12	83,821,454 (GRCm39)	missense	probably damaging	1.00
R6035:Papln	UTSW	12	83,821,454 (GRCm39)	missense	probably damaging	1.00
R6291:Papln	UTSW	12	83,829,789 (GRCm39)	missense	probably benign	0.01
R6461:Papln	UTSW	12	83,828,587 (GRCm39)	splice site	probably null
R6536:Papln	UTSW	12	83,828,661 (GRCm39)	missense	probably damaging	1.00
R6861:Papln	UTSW	12	83,821,723 (GRCm39)	missense	probably damaging	1.00
R6898:Papln	UTSW	12	83,824,234 (GRCm39)	missense	probably benign	0.03
R6953:Papln	UTSW	12	83,828,659 (GRCm39)	nonsense	probably null
R7155:Papln	UTSW	12	83,823,295 (GRCm39)	missense	probably damaging	1.00
R7450:Papln	UTSW	12	83,826,945 (GRCm39)	missense	probably benign	0.13
R7510:Papln	UTSW	12	83,818,947 (GRCm39)	missense	probably damaging	0.99
R7850:Papln	UTSW	12	83,827,436 (GRCm39)	missense	probably damaging	1.00
R7977:Papln	UTSW	12	83,822,156 (GRCm39)	missense	probably damaging	1.00
R7987:Papln	UTSW	12	83,822,156 (GRCm39)	missense	probably damaging	1.00
R8321:Papln	UTSW	12	83,821,715 (GRCm39)	nonsense	probably null
R8324:Papln	UTSW	12	83,833,393 (GRCm39)	missense	probably damaging	1.00
R8466:Papln	UTSW	12	83,825,255 (GRCm39)	critical splice acceptor site	probably null
R8743:Papln	UTSW	12	83,829,764 (GRCm39)	missense	probably damaging	1.00
R8790:Papln	UTSW	12	83,823,918 (GRCm39)	missense	probably benign	0.01
R9086:Papln	UTSW	12	83,821,633 (GRCm39)	missense	probably damaging	1.00
R9291:Papln	UTSW	12	83,825,284 (GRCm39)	missense	probably benign	0.01
R9350:Papln	UTSW	12	83,833,638 (GRCm39)	missense	probably damaging	1.00
R9438:Papln	UTSW	12	83,818,606 (GRCm39)	missense	probably benign
R9484:Papln	UTSW	12	83,838,618 (GRCm39)	missense	probably benign	0.05
V7580:Papln	UTSW	12	83,825,608 (GRCm39)	missense	possibly damaging	0.72
V7581:Papln	UTSW	12	83,825,608 (GRCm39)	missense	possibly damaging	0.72
V7582:Papln	UTSW	12	83,825,608 (GRCm39)	missense	possibly damaging	0.72
Z1088:Papln	UTSW	12	83,823,150 (GRCm39)	missense	probably benign	0.19

Predicted Primers

PCR Primer
(F):5'- GGTGACATCTTCCATCCCCAAC -3'
(R):5'- TCTGTCCAGTCCAGGAACTC -3'

Sequencing Primer
(F):5'- CCCACAGCTTACCCAGTG -3'
(R):5'- AGGAACTCTGGGCCGGATTTC -3'

Posted On

2015-10-21