Incidental Mutation 'R4703:Limk2'
ID 356230
Institutional Source Beutler Lab
Gene Symbol Limk2
Ensembl Gene ENSMUSG00000020451
Gene Name LIM domain kinase 2
Synonyms whe, Limk2b, Limk2a, A930024P04Rik, LIM kinase 2
MMRRC Submission 041951-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.186) question?
Stock # R4703 (G1)
Quality Score 225
Status Validated
Chromosome 11
Chromosomal Location 3294256-3359189 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 3297586 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 329 (E329*)
Ref Sequence ENSEMBL: ENSMUSP00000105656 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000045153] [ENSMUST00000101638] [ENSMUST00000101640] [ENSMUST00000101642] [ENSMUST00000110029]
AlphaFold O54785
Predicted Effect probably benign
Transcript: ENSMUST00000045153
SMART Domains Protein: ENSMUSP00000036921
Gene: ENSMUSG00000034614

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
KR 23 103 3.64e-17 SMART
transmembrane domain 170 192 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000101638
AA Change: E516*
SMART Domains Protein: ENSMUSP00000099162
Gene: ENSMUSG00000020451
AA Change: E516*

DomainStartEndE-ValueType
LIM 11 63 2e-14 SMART
LIM 71 124 4.63e-10 SMART
PDZ 161 239 7.04e-10 SMART
low complexity region 241 255 N/A INTRINSIC
low complexity region 280 306 N/A INTRINSIC
low complexity region 310 322 N/A INTRINSIC
Pfam:Pkinase 331 600 5.3e-48 PFAM
Pfam:Pkinase_Tyr 331 601 4.7e-50 PFAM
Pfam:Kdo 341 497 8.6e-7 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000101640
AA Change: E501*
SMART Domains Protein: ENSMUSP00000099163
Gene: ENSMUSG00000020451
AA Change: E501*

DomainStartEndE-ValueType
LIM 7 42 4.91e-1 SMART
LIM 50 103 4.63e-10 SMART
PDZ 140 218 7.04e-10 SMART
low complexity region 220 234 N/A INTRINSIC
low complexity region 259 285 N/A INTRINSIC
low complexity region 289 301 N/A INTRINSIC
Pfam:Pkinase 310 582 1.2e-45 PFAM
Pfam:Pkinase_Tyr 310 586 1.3e-51 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000101642
AA Change: E495*
SMART Domains Protein: ENSMUSP00000099165
Gene: ENSMUSG00000020451
AA Change: E495*

DomainStartEndE-ValueType
LIM 7 42 4.91e-1 SMART
LIM 50 103 4.63e-10 SMART
PDZ 140 218 7.04e-10 SMART
low complexity region 220 234 N/A INTRINSIC
low complexity region 259 285 N/A INTRINSIC
low complexity region 289 301 N/A INTRINSIC
Pfam:Pkinase 310 579 4.9e-48 PFAM
Pfam:Pkinase_Tyr 310 580 4.3e-50 PFAM
Pfam:Kdo 320 476 8.2e-7 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000110029
AA Change: E329*
SMART Domains Protein: ENSMUSP00000105656
Gene: ENSMUSG00000020451
AA Change: E329*

DomainStartEndE-ValueType
PDZ 1 52 4.55e-1 SMART
low complexity region 54 68 N/A INTRINSIC
low complexity region 93 119 N/A INTRINSIC
low complexity region 123 135 N/A INTRINSIC
Pfam:Pkinase 144 411 2.7e-49 PFAM
Pfam:Pkinase_Tyr 144 414 1.7e-51 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134576
Predicted Effect noncoding transcript
Transcript: ENSMUST00000148771
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 94.6%
Validation Efficiency 97% (98/101)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Male homozygotes for targeted null mutations exhibit small testes but are fertile. Mutant kidneys have fewer glomeruli and dilated renal tubules, but function normally. Mice homozygous for a gene trap allele or spontaneous mutation have open eyelids at birth, corneal abnormalities and inflammation. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 89 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921517D22Rik T A 13: 59,837,342 (GRCm39) T248S possibly damaging Het
AA986860 T C 1: 130,671,092 (GRCm39) V438A probably benign Het
Adam25 G T 8: 41,207,163 (GRCm39) C143F probably damaging Het
Aox1 T A 1: 58,398,116 (GRCm39) F1286I possibly damaging Het
Apobec4 A G 1: 152,632,001 (GRCm39) T10A probably benign Het
Arhgap5 C T 12: 52,564,366 (GRCm39) P446S probably damaging Het
Arhgef40 A G 14: 52,239,767 (GRCm39) N1327S probably damaging Het
Armc12 A G 17: 28,751,336 (GRCm39) D110G probably benign Het
Ascc1 A G 10: 59,885,624 (GRCm39) Y225C probably damaging Het
Aspscr1 A T 11: 120,579,771 (GRCm39) K39N possibly damaging Het
B4galnt1 G T 10: 127,003,394 (GRCm39) V172F possibly damaging Het
B4galt1 A G 4: 40,823,569 (GRCm39) V174A probably benign Het
Bcl11a C A 11: 24,113,725 (GRCm39) A356E possibly damaging Het
Bri3bp C T 5: 125,528,830 (GRCm39) L110F probably damaging Het
Cacna1b T C 2: 24,544,475 (GRCm39) D1231G probably damaging Het
Ccdc33 T A 9: 57,940,953 (GRCm39) I430F possibly damaging Het
Cgn A G 3: 94,683,405 (GRCm39) probably benign Het
Crbn T A 6: 106,759,883 (GRCm39) I317F possibly damaging Het
Cyp2d22 A C 15: 82,260,118 (GRCm39) L22R probably damaging Het
Dnah7a C T 1: 53,486,476 (GRCm39) probably null Het
Dnajc12 A G 10: 63,222,429 (GRCm39) probably null Het
Dntt T A 19: 41,028,242 (GRCm39) D179E probably benign Het
Enam T A 5: 88,651,650 (GRCm39) L1053* probably null Het
Epn1 T A 7: 5,098,147 (GRCm39) D319E probably damaging Het
Evpl C G 11: 116,113,331 (GRCm39) R1453P probably damaging Het
Focad T A 4: 88,260,558 (GRCm39) probably null Het
Foxp2 A T 6: 15,411,247 (GRCm39) M542L probably benign Het
Gak T A 5: 108,717,743 (GRCm39) Q1299L probably damaging Het
Galnt5 G T 2: 57,888,919 (GRCm39) R173I possibly damaging Het
Gli1 G T 10: 127,166,724 (GRCm39) P843Q possibly damaging Het
Gm5422 G T 10: 31,125,608 (GRCm39) noncoding transcript Het
Gna14 T G 19: 16,576,344 (GRCm39) V117G possibly damaging Het
Gpr6 T C 10: 40,947,037 (GRCm39) T182A probably damaging Het
Ifi204 C A 1: 173,587,927 (GRCm39) probably benign Het
Ifih1 A T 2: 62,429,220 (GRCm39) L906H probably benign Het
Ift88 A G 14: 57,718,307 (GRCm39) probably benign Het
Ighd A G 12: 113,379,661 (GRCm39) probably benign Het
Ighv11-1 A C 12: 113,945,622 (GRCm39) I77R possibly damaging Het
Il22 A T 10: 118,041,511 (GRCm39) I75F probably damaging Het
Il23r A T 6: 67,467,686 (GRCm39) I27K probably damaging Het
Inpp5a A C 7: 139,138,839 (GRCm39) N261T probably damaging Het
Ints8 T G 4: 11,223,785 (GRCm39) Q686P possibly damaging Het
Iqcf4 T C 9: 106,445,519 (GRCm39) probably null Het
Irf2bp1 C T 7: 18,739,496 (GRCm39) R379C possibly damaging Het
Iws1 C T 18: 32,213,066 (GRCm39) P165S probably benign Het
Kalrn T C 16: 34,024,327 (GRCm39) D610G probably damaging Het
Kcna10 A T 3: 107,101,926 (GRCm39) I186F probably benign Het
Nadk C A 4: 155,669,684 (GRCm39) P157T probably benign Het
Notch1 T G 2: 26,361,170 (GRCm39) K1107Q probably benign Het
Nsd1 T A 13: 55,361,876 (GRCm39) D281E probably damaging Het
Obi1 A G 14: 104,743,644 (GRCm39) L145P probably benign Het
Olfml2a T A 2: 38,841,250 (GRCm39) L262Q probably damaging Het
Or1ab2 T A 8: 72,864,044 (GRCm39) F211L probably damaging Het
Or4k44 T A 2: 111,368,113 (GRCm39) I174L probably benign Het
Or51d1 A T 7: 102,347,477 (GRCm39) T11S probably benign Het
Or6k6 A G 1: 173,944,964 (GRCm39) I206T possibly damaging Het
Or7c19 A G 8: 85,957,237 (GRCm39) T38A possibly damaging Het
Otogl A C 10: 107,657,785 (GRCm39) D1048E probably damaging Het
Oxnad1 T C 14: 31,817,427 (GRCm39) W96R probably damaging Het
Pcdh15 A T 10: 74,285,995 (GRCm39) D743V probably damaging Het
Pclo A G 5: 14,726,494 (GRCm39) probably benign Het
Pcnx1 C T 12: 81,941,938 (GRCm39) T112I probably benign Het
Pctp T C 11: 89,878,099 (GRCm39) E145G possibly damaging Het
Pip5k1b T C 19: 24,332,517 (GRCm39) K389R probably damaging Het
Pla2g15 T A 8: 106,889,691 (GRCm39) M321K probably benign Het
Pnlip T A 19: 58,664,899 (GRCm39) D242E probably damaging Het
Ptpn21 T C 12: 98,645,651 (GRCm39) T1096A probably benign Het
Rims3 A T 4: 120,740,494 (GRCm39) probably benign Het
Scfd2 T A 5: 74,680,256 (GRCm39) Q299L probably benign Het
Selplg T C 5: 113,957,094 (GRCm39) D404G probably benign Het
Slc15a5 T C 6: 138,032,643 (GRCm39) D237G probably benign Het
Slc16a12 T A 19: 34,652,291 (GRCm39) H285L possibly damaging Het
Sox2 C A 3: 34,704,862 (GRCm39) R100S probably damaging Het
Sspo G A 6: 48,477,387 (GRCm39) C4969Y probably damaging Het
Stxbp2 A G 8: 3,682,521 (GRCm39) S37G probably damaging Het
Tbxas1 T C 6: 39,060,791 (GRCm39) probably null Het
Tcf4 A G 18: 69,790,981 (GRCm39) Y307C probably damaging Het
Thsd7b A T 1: 129,977,646 (GRCm39) probably benign Het
Tnn G T 1: 159,943,815 (GRCm39) D999E possibly damaging Het
Trmt13 C A 3: 116,388,247 (GRCm39) W63L probably benign Het
Tsc2 T C 17: 24,823,883 (GRCm39) N915S probably benign Het
Tyrp1 T A 4: 80,759,043 (GRCm39) probably null Het
Uvrag A T 7: 98,638,794 (GRCm39) I315N probably damaging Het
Vmn1r31 C A 6: 58,448,953 (GRCm39) *304L probably null Het
Vmn2r59 T A 7: 41,661,686 (GRCm39) I710L probably benign Het
Vmn2r82 A T 10: 79,214,641 (GRCm39) H208L probably damaging Het
Wtap T C 17: 13,199,711 (GRCm39) T91A probably benign Het
Xirp1 A T 9: 119,846,093 (GRCm39) V930E probably damaging Het
Xpo4 T G 14: 57,827,565 (GRCm39) H877P probably benign Het
Other mutations in Limk2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01105:Limk2 APN 11 3,305,475 (GRCm39) splice site probably benign
IGL01592:Limk2 APN 11 3,309,052 (GRCm39) missense probably benign 0.00
IGL01716:Limk2 APN 11 3,308,990 (GRCm39) splice site probably null
IGL01911:Limk2 APN 11 3,305,340 (GRCm39) missense probably benign
R0900:Limk2 UTSW 11 3,300,731 (GRCm39) missense probably damaging 1.00
R1587:Limk2 UTSW 11 3,303,455 (GRCm39) missense possibly damaging 0.82
R1632:Limk2 UTSW 11 3,296,250 (GRCm39) missense probably damaging 1.00
R1695:Limk2 UTSW 11 3,303,275 (GRCm39) critical splice donor site probably null
R1712:Limk2 UTSW 11 3,308,104 (GRCm39) splice site probably null
R1792:Limk2 UTSW 11 3,308,236 (GRCm39) missense probably benign
R1982:Limk2 UTSW 11 3,305,461 (GRCm39) missense probably benign 0.00
R3009:Limk2 UTSW 11 3,309,046 (GRCm39) missense probably benign 0.01
R4565:Limk2 UTSW 11 3,298,634 (GRCm39) missense probably damaging 0.98
R4978:Limk2 UTSW 11 3,359,069 (GRCm39) utr 5 prime probably benign
R5160:Limk2 UTSW 11 3,300,772 (GRCm39) missense probably damaging 1.00
R5460:Limk2 UTSW 11 3,302,332 (GRCm39) missense probably benign 0.30
R6497:Limk2 UTSW 11 3,310,492 (GRCm39) missense probably benign 0.00
R6543:Limk2 UTSW 11 3,300,682 (GRCm39) missense probably damaging 1.00
R6666:Limk2 UTSW 11 3,310,493 (GRCm39) missense probably damaging 1.00
R7054:Limk2 UTSW 11 3,305,448 (GRCm39) missense possibly damaging 0.95
R7330:Limk2 UTSW 11 3,296,311 (GRCm39) missense probably benign 0.39
R7681:Limk2 UTSW 11 3,303,354 (GRCm39) missense probably damaging 0.96
R7722:Limk2 UTSW 11 3,306,092 (GRCm39) splice site probably null
R7745:Limk2 UTSW 11 3,305,896 (GRCm39) missense probably damaging 0.99
R8120:Limk2 UTSW 11 3,298,589 (GRCm39) splice site probably null
R8193:Limk2 UTSW 11 3,297,691 (GRCm39) missense possibly damaging 0.79
R8379:Limk2 UTSW 11 3,321,162 (GRCm39) start gained probably benign
R8557:Limk2 UTSW 11 3,296,379 (GRCm39) missense possibly damaging 0.89
R8708:Limk2 UTSW 11 3,300,763 (GRCm39) missense probably benign 0.19
R9617:Limk2 UTSW 11 3,297,715 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- GATTAAAAGTTTATGAGGCCTGGC -3'
(R):5'- TTGAAGGTGGCCTCTCTTGC -3'

Sequencing Primer
(F):5'- TGAAAGATCATCGGCCTC -3'
(R):5'- AAAGGCCTCCAGTAGAGA -3'
Posted On 2015-10-21