Mutagenetix > Incidental Mutation

Incidental Mutation 'R4704:Wnk1'

356288

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

Prkwnk1, 6430573H23Rik, EG406236, Hsn2

MMRRC Submission

041952-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4704 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119900930-120015633 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 119942705 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Serine at position 774 (L774S)

Ref Sequence

ENSEMBL: ENSMUSP00000136777 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000160686] [ENSMUST00000177761] [ENSMUST00000203030] [ENSMUST00000161512]

AlphaFold

P83741

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000035513

Predicted Effect

probably benign
Transcript: ENSMUST00000060043

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088644

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088646

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

probably benign
Transcript: ENSMUST00000160686

SMART Domains

Protein: ENSMUSP00000137334
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	102	115	N/A	INTRINSIC
low complexity region	119	128	N/A	INTRINSIC
low complexity region	198	210	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160769

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177761
AA Change: L774S

PolyPhen 2 Score 0.835 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: L774S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161392

Predicted Effect

probably benign
Transcript: ENSMUST00000203030

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162368

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161048

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161512

SMART Domains

Protein: ENSMUSP00000136086
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	40	51	N/A	INTRINSIC
low complexity region	217	233	N/A	INTRINSIC
low complexity region	348	361	N/A	INTRINSIC
low complexity region	365	374	N/A	INTRINSIC

Meta Mutation Damage Score

0.0860

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 94.0%

Validation Efficiency

97% (89/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057M21Rik	A	T	7: 130,959,259 (GRCm39)	M147K	probably damaging	Het
Abca13	A	G	11: 9,226,990 (GRCm39)	D582G	possibly damaging	Het
Abca2	T	C	2: 25,333,424 (GRCm39)	L1683P	probably damaging	Het
Adam39	A	G	8: 41,278,833 (GRCm39)	H408R	probably benign	Het
Adcy4	A	G	14: 56,012,482 (GRCm39)	S554P	possibly damaging	Het
Ahnak	T	C	19: 8,990,545 (GRCm39)	I3943T	probably damaging	Het
Ahnak	T	G	19: 8,989,622 (GRCm39)		probably benign	Het
Alkal2	T	A	12: 30,937,195 (GRCm39)	S109R	probably damaging	Het
Apobec4	A	G	1: 152,632,001 (GRCm39)	T10A	probably benign	Het
Arhgef40	A	G	14: 52,239,767 (GRCm39)	N1327S	probably damaging	Het
Arpc3	A	G	5: 122,538,471 (GRCm39)	M1V	probably null	Het
Ascc1	A	G	10: 59,885,624 (GRCm39)	Y225C	probably damaging	Het
Ascc3	A	G	10: 50,535,110 (GRCm39)	I668V	probably benign	Het
Bdnf	T	A	2: 109,554,037 (GRCm39)	M137K	possibly damaging	Het
Cacna1b	T	C	2: 24,544,475 (GRCm39)	D1231G	probably damaging	Het
Cds2	T	A	2: 132,142,522 (GRCm39)	Y239*	probably null	Het
Cpped1	G	A	16: 11,703,493 (GRCm39)		probably benign	Het
Ctu2	G	A	8: 123,206,042 (GRCm39)	R261Q	probably damaging	Het
Cux1	A	G	5: 136,278,055 (GRCm39)	V645A	probably benign	Het
Cyp4f13	A	G	17: 33,144,709 (GRCm39)	C401R	probably damaging	Het
Dpt	T	G	1: 164,646,518 (GRCm39)	Y162*	probably null	Het
Ednra	A	G	8: 78,394,592 (GRCm39)		probably benign	Het
Eepd1	A	G	9: 25,394,122 (GRCm39)	T129A	probably benign	Het
Eif2s1	A	G	12: 78,923,944 (GRCm39)	T134A	probably benign	Het
Eloa	A	G	4: 135,738,525 (GRCm39)	V145A	probably benign	Het
Enam	T	A	5: 88,651,650 (GRCm39)	L1053*	probably null	Het
Eng	T	C	2: 32,568,924 (GRCm39)	S484P	probably benign	Het
Eogt	A	T	6: 97,090,813 (GRCm39)	V442E	probably damaging	Het
Fam185a	C	T	5: 21,685,471 (GRCm39)		probably benign	Het
Gm4922	C	T	10: 18,660,567 (GRCm39)	V52I	probably benign	Het
Gnao1	A	G	8: 94,538,004 (GRCm39)	E14G	probably benign	Het
Gpr75	C	A	11: 30,841,110 (GRCm39)	A5D	probably benign	Het
Hbq1b	T	A	11: 32,237,448 (GRCm39)		probably benign	Het
Hdac7	G	A	15: 97,694,097 (GRCm39)	T724M	probably damaging	Het
Ifi204	C	A	1: 173,587,927 (GRCm39)		probably benign	Het
Ift88	A	G	14: 57,718,307 (GRCm39)		probably benign	Het
Irak4	A	G	15: 94,464,781 (GRCm39)		probably null	Het
Kalrn	T	C	16: 34,024,327 (GRCm39)	D610G	probably damaging	Het
Kifc2	T	C	15: 76,547,177 (GRCm39)		probably null	Het
Kmt2c	G	A	5: 25,519,025 (GRCm39)	Q2362*	probably null	Het
Kntc1	G	A	5: 123,949,496 (GRCm39)	E1956K	probably damaging	Het
Lama3	T	C	18: 12,686,280 (GRCm39)	V2781A	probably benign	Het
Lipt2	A	G	7: 99,809,534 (GRCm39)	E207G	probably damaging	Het
Mag	A	T	7: 30,608,598 (GRCm39)	L172Q	probably damaging	Het
Map1b	A	T	13: 99,566,983 (GRCm39)	C1913S	unknown	Het
Mical3	A	G	6: 120,935,649 (GRCm39)	S1626P	probably benign	Het
Mslnl	G	T	17: 25,957,952 (GRCm39)	W65L	possibly damaging	Het
Muc20	G	T	16: 32,599,448 (GRCm39)	A3332S	possibly damaging	Het
Nadk	C	A	4: 155,669,684 (GRCm39)	P157T	probably benign	Het
Nkx2-3	G	A	19: 43,601,123 (GRCm39)	E62K	probably damaging	Het
Or6c2	T	C	10: 129,362,171 (GRCm39)	L25P	possibly damaging	Het
Pclo	A	G	5: 14,726,494 (GRCm39)		probably benign	Het
Pcna-ps2	T	A	19: 9,260,786 (GRCm39)	V15E	possibly damaging	Het
Plekhg3	G	T	12: 76,625,012 (GRCm39)	G1285W	probably damaging	Het
Pramel29	A	T	4: 143,935,162 (GRCm39)	I193N	probably damaging	Het
Procr	A	G	2: 155,596,258 (GRCm39)	S142G	probably damaging	Het
Ptpn3	A	T	4: 57,270,119 (GRCm39)	N14K	possibly damaging	Het
Rin1	C	A	19: 5,105,018 (GRCm39)	L693I	probably damaging	Het
Ripk4	C	A	16: 97,547,204 (GRCm39)	E290*	probably null	Het
Rnf213	A	G	11: 119,331,175 (GRCm39)	Y2128C	probably damaging	Het
Saal1	T	C	7: 46,349,164 (GRCm39)		probably benign	Het
Selplg	T	C	5: 113,957,094 (GRCm39)	D404G	probably benign	Het
Serpinf1	C	A	11: 75,301,867 (GRCm39)	A263S	probably damaging	Het
Sgo2b	A	T	8: 64,380,824 (GRCm39)	D669E	probably damaging	Het
Slc30a9	T	C	5: 67,499,616 (GRCm39)		probably benign	Het
Sri	A	T	5: 8,112,430 (GRCm39)		probably null	Het
Sspo	G	A	6: 48,475,638 (GRCm39)	C4918Y	probably damaging	Het
Szt2	A	G	4: 118,251,026 (GRCm39)	Y361H	probably damaging	Het
Tbc1d12	T	A	19: 38,889,781 (GRCm39)	W404R	probably damaging	Het
Tcf20	T	C	15: 82,735,928 (GRCm39)	E1841G	possibly damaging	Het
Tep1	T	C	14: 51,074,530 (GRCm39)	T1832A	probably benign	Het
Tmem132e	G	A	11: 82,334,357 (GRCm39)	A623T	probably damaging	Het
Tmem201	A	T	4: 149,811,774 (GRCm39)	F357Y	possibly damaging	Het
Trappc13	T	G	13: 104,303,329 (GRCm39)		probably benign	Het
Trav6-5	C	T	14: 53,728,883 (GRCm39)	Q47*	probably null	Het
Ubxn1	T	A	19: 8,849,399 (GRCm39)	D47E	probably benign	Het
Vmn2r45	G	T	7: 8,486,535 (GRCm39)	S251*	probably null	Het
Zfp189	T	C	4: 49,530,081 (GRCm39)	S395P	probably damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119,937,669 (GRCm39)	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119,914,372 (GRCm39)	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119,925,358 (GRCm39)	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119,940,446 (GRCm39)	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119,926,039 (GRCm39)	unclassified	probably benign
IGL02268:Wnk1	APN	6	119,914,334 (GRCm39)	nonsense	probably null
IGL02348:Wnk1	APN	6	119,940,289 (GRCm39)	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119,940,415 (GRCm39)	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119,914,823 (GRCm39)	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119,903,594 (GRCm39)	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119,931,148 (GRCm39)	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119,930,856 (GRCm39)	missense	probably benign	0.41
Actor	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
Bad	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
Blink	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
Knock	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
Narrow	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
nictitate	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
opportunity	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
path	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
Stormy	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
tear	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
Tic	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
Unforgiving	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
Window	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
Woke	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119,921,760 (GRCm39)	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119,940,328 (GRCm39)	nonsense	probably null
R0044:Wnk1	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119,929,694 (GRCm39)	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119,905,124 (GRCm39)	intron	probably benign
R0453:Wnk1	UTSW	6	119,940,112 (GRCm39)	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119,946,293 (GRCm39)	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119,939,764 (GRCm39)	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119,903,525 (GRCm39)	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119,928,953 (GRCm39)	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119,903,644 (GRCm39)	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119,925,466 (GRCm39)	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119,928,967 (GRCm39)	splice site	probably benign
R1188:Wnk1	UTSW	6	119,925,670 (GRCm39)	nonsense	probably null
R1245:Wnk1	UTSW	6	119,925,418 (GRCm39)	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119,927,645 (GRCm39)	splice site	probably benign
R1869:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119,929,884 (GRCm39)	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119,911,343 (GRCm39)	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119,914,539 (GRCm39)	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119,905,118 (GRCm39)	splice site	probably null
R2144:Wnk1	UTSW	6	119,925,949 (GRCm39)	unclassified	probably benign
R2186:Wnk1	UTSW	6	119,925,528 (GRCm39)	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119,940,601 (GRCm39)	splice site	probably null
R2338:Wnk1	UTSW	6	119,946,495 (GRCm39)	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119,913,328 (GRCm39)	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119,946,315 (GRCm39)	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119,926,012 (GRCm39)	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119,925,087 (GRCm39)	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119,926,222 (GRCm39)	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119,930,856 (GRCm39)	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119,903,387 (GRCm39)	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119,928,872 (GRCm39)	missense	possibly damaging	0.49
R4755:Wnk1	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119,929,732 (GRCm39)	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119,939,399 (GRCm39)	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119,926,338 (GRCm39)	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5002:Wnk1	UTSW	6	119,914,924 (GRCm39)	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5152:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120,014,149 (GRCm39)	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119,945,484 (GRCm39)	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119,925,852 (GRCm39)	unclassified	probably benign
R5600:Wnk1	UTSW	6	119,926,319 (GRCm39)	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120,014,562 (GRCm39)	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119,949,958 (GRCm39)	intron	probably benign
R6128:Wnk1	UTSW	6	119,940,747 (GRCm39)	splice site	probably null
R6237:Wnk1	UTSW	6	119,929,728 (GRCm39)	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119,925,546 (GRCm39)	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119,925,742 (GRCm39)	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
R7024:Wnk1	UTSW	6	119,942,687 (GRCm39)	intron	probably benign
R7072:Wnk1	UTSW	6	119,914,822 (GRCm39)	missense	unknown
R7087:Wnk1	UTSW	6	120,014,491 (GRCm39)	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119,925,268 (GRCm39)	missense	unknown
R7134:Wnk1	UTSW	6	119,903,389 (GRCm39)	missense	unknown
R7137:Wnk1	UTSW	6	120,015,173 (GRCm39)	unclassified	probably benign
R7142:Wnk1	UTSW	6	119,926,240 (GRCm39)	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
R7218:Wnk1	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
R7498:Wnk1	UTSW	6	119,904,157 (GRCm39)	missense	unknown
R7599:Wnk1	UTSW	6	119,906,789 (GRCm39)	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119,909,699 (GRCm39)	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119,926,137 (GRCm39)	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120,014,409 (GRCm39)	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119,909,675 (GRCm39)	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119,930,794 (GRCm39)	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119,940,454 (GRCm39)	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119,906,960 (GRCm39)	splice site	probably null
R8359:Wnk1	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119,911,388 (GRCm39)	missense	unknown
R8519:Wnk1	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120,013,959 (GRCm39)	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119,939,393 (GRCm39)	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119,925,631 (GRCm39)	missense	unknown
R9148:Wnk1	UTSW	6	119,925,631 (GRCm39)	missense	unknown
R9354:Wnk1	UTSW	6	119,942,660 (GRCm39)	missense	unknown
R9379:Wnk1	UTSW	6	119,928,678 (GRCm39)	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120,013,993 (GRCm39)	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119,925,166 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGGTAGACTCTACTTTTGATTAGGC -3'
(R):5'- GGGGAAGGTAGAACATTTCTTTATG -3'

Sequencing Primer
(F):5'- AGTTATCTTAAAAGCACAACATTTGC -3'
(R):5'- GTTTAGCCTCGTCGTGGCC -3'

Posted On

2015-10-21