Mutagenetix > Incidental Mutation

Incidental Mutation 'R4745:Lama1'

356689

Institutional Source

Beutler Lab

Gene Symbol

Lama1

Ensembl Gene

ENSMUSG00000032796

Gene Name

laminin, alpha 1

Synonyms

Lama

MMRRC Submission

042028-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4745 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

68004254-68129642 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 68045775 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 227 (S227P)

Ref Sequence

ENSEMBL: ENSMUSP00000043957 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035471]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000035471
AA Change: S227P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000043957
Gene: ENSMUSG00000032796
AA Change: S227P

Domain	Start	End	E-Value	Type
low complexity region	3	22	N/A	INTRINSIC
LamNT	23	275	1.2e-131	SMART
EGF_Lam	277	331	1e-5	SMART
EGF_Lam	334	401	6.6e-6	SMART
EGF_Lam	404	458	9.11e-9	SMART
EGF_Lam	461	507	8.12e-6	SMART
LamB	570	702	2.09e-57	SMART
EGF_like	715	746	3.36e0	SMART
EGF_Lam	749	795	7.01e-10	SMART
EGF_Lam	798	853	3.59e-7	SMART
EGF_Lam	856	906	1.53e-10	SMART
EGF_Lam	909	955	1.13e-13	SMART
EGF_Lam	958	1002	1.36e-7	SMART
EGF_Lam	1005	1048	7.29e-8	SMART
EGF_like	1034	1082	4.83e1	SMART
EGF_Lam	1051	1094	1.67e-7	SMART
EGF_Lam	1097	1154	1.32e-5	SMART
LamB	1220	1352	8.7e-46	SMART
Pfam:Laminin_EGF	1367	1397	1.7e-6	PFAM
EGF_Lam	1410	1456	7.12e-11	SMART
EGF_Lam	1459	1513	3.25e-5	SMART
EGF_like	1497	1547	6.41e1	SMART
EGF_Lam	1516	1560	1.71e-13	SMART
Pfam:Laminin_I	1574	1838	1.7e-91	PFAM
low complexity region	2012	2031	N/A	INTRINSIC
low complexity region	2087	2098	N/A	INTRINSIC
LamG	2145	2287	3.66e-30	SMART
LamG	2332	2473	5.98e-35	SMART
LamG	2513	2661	1.11e-29	SMART
low complexity region	2695	2708	N/A	INTRINSIC
LamG	2743	2877	9.72e-35	SMART
LamG	2920	3056	4.63e-41	SMART

Meta Mutation Damage Score

0.6835

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

97% (89/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]
PHENOTYPE: Homozygous null mice display embryonic lethality before somite formation with impaired formation of Reichert's membrane. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	T	C	17: 24,526,427 (GRCm39)	Y619C	probably damaging	Het
Adck1	A	G	12: 88,368,949 (GRCm39)		probably null	Het
Agap3	A	C	5: 24,656,123 (GRCm39)		probably null	Het
Ankib1	A	T	5: 3,782,566 (GRCm39)	H354Q	probably damaging	Het
Ankrd29	T	G	18: 12,387,679 (GRCm39)	N301T	probably benign	Het
Arhgef4	A	G	1: 34,846,356 (GRCm39)	T379A	probably damaging	Het
Arid1a	A	G	4: 133,480,417 (GRCm39)	V169A	probably benign	Het
Bag2	T	C	1: 33,787,417 (GRCm39)		probably null	Het
Bmt2	A	C	6: 13,628,686 (GRCm39)	Y332*	probably null	Het
Bpifb1	A	T	2: 154,053,501 (GRCm39)	K248*	probably null	Het
Caap1	C	A	4: 94,444,751 (GRCm39)		probably null	Het
Calcr	T	A	6: 3,692,576 (GRCm39)	Y389F	probably damaging	Het
Capn1	C	T	19: 6,043,946 (GRCm39)	V562I	probably benign	Het
Ccr1	T	A	9: 123,763,985 (GRCm39)	T182S	probably benign	Het
Ceacam15	T	C	7: 16,407,259 (GRCm39)	D86G	probably benign	Het
Cldnd1	C	T	16: 58,550,006 (GRCm39)	T63I	probably benign	Het
Col12a1	A	T	9: 79,559,368 (GRCm39)		probably null	Het
Cystm1	A	G	18: 36,526,348 (GRCm39)		probably benign	Het
Ddx55	T	A	5: 124,705,028 (GRCm39)	Y428*	probably null	Het
Ensa	G	A	3: 95,538,745 (GRCm39)	G118D	probably benign	Het
Folh1	A	T	7: 86,372,482 (GRCm39)		probably null	Het
Foxj2	C	A	6: 122,814,948 (GRCm39)	P328Q	probably damaging	Het
Fscn3	A	G	6: 28,435,627 (GRCm39)	I417V	probably damaging	Het
Galnt7	T	C	8: 57,995,761 (GRCm39)		probably benign	Het
Gm11563	T	A	11: 99,549,246 (GRCm39)	*169C	probably null	Het
Hfm1	A	T	5: 107,049,709 (GRCm39)	D417E	possibly damaging	Het
Ighv15-2	A	G	12: 114,528,230 (GRCm39)	S107P	probably damaging	Het
Itsn2	A	G	12: 4,711,944 (GRCm39)	D904G	probably damaging	Het
Kif1b	A	T	4: 149,322,339 (GRCm39)	L860*	probably null	Het
Krt79	T	C	15: 101,839,119 (GRCm39)	E450G	probably damaging	Het
Lamp5	C	A	2: 135,902,786 (GRCm39)	H168Q	probably benign	Het
Lilra5	A	T	7: 4,245,076 (GRCm39)	Q240L	possibly damaging	Het
Lrp1	A	T	10: 127,385,813 (GRCm39)	C3521S	probably benign	Het
Mroh1	T	A	15: 76,292,730 (GRCm39)		probably null	Het
Nlrp4g	A	T	9: 124,349,515 (GRCm38)		noncoding transcript	Het
Nr2f6	A	T	8: 71,831,179 (GRCm39)	I70N	probably benign	Het
Nr4a2	T	A	2: 57,000,163 (GRCm39)	D311V	probably damaging	Het
Odad2	G	A	18: 7,286,763 (GRCm39)	T156M	probably benign	Het
Or10w1	T	A	19: 13,632,750 (GRCm39)	M319K	probably benign	Het
Or10x4	T	C	1: 174,219,442 (GRCm39)	L269P	probably damaging	Het
Or52n20	T	A	7: 104,320,711 (GRCm39)	F267L	probably damaging	Het
Pcdhb6	C	A	18: 37,468,426 (GRCm39)	A449D	possibly damaging	Het
Pcgf6	A	G	19: 47,036,545 (GRCm39)		probably null	Het
Prc1	C	A	7: 79,962,911 (GRCm39)	H131Q	probably benign	Het
Ptprq	C	A	10: 107,360,114 (GRCm39)	R2187L	probably damaging	Het
Rasl2-9	C	A	7: 5,128,702 (GRCm39)	R76L	possibly damaging	Het
Rdh16f1	A	T	10: 127,626,685 (GRCm39)	Y246F	probably benign	Het
Rit1	T	C	3: 88,624,982 (GRCm39)		probably benign	Het
Sash1	A	G	10: 8,605,672 (GRCm39)	V906A	probably benign	Het
Scnn1b	T	C	7: 121,501,509 (GRCm39)	V108A	probably benign	Het
Sema4f	A	T	6: 82,895,265 (GRCm39)	I356N	probably damaging	Het
Shc4	A	T	2: 125,491,197 (GRCm39)	L447Q	probably damaging	Het
Slc24a1	T	C	9: 64,856,758 (GRCm39)	M50V	unknown	Het
Slc28a3	T	A	13: 58,722,077 (GRCm39)	D269V	possibly damaging	Het
Slc35e1	A	G	8: 73,246,166 (GRCm39)	S89P	possibly damaging	Het
Smpd5	T	A	15: 76,179,008 (GRCm39)	H125Q	probably benign	Het
Snapc2	A	G	8: 4,304,578 (GRCm39)	T31A	probably damaging	Het
Sox5	G	C	6: 143,779,214 (GRCm39)	H606D	possibly damaging	Het
Spag6	A	G	2: 18,742,107 (GRCm39)	T367A	possibly damaging	Het
Spag8	T	C	4: 43,651,636 (GRCm39)	T413A	probably damaging	Het
Sptlc3	G	A	2: 139,389,087 (GRCm39)	G156R	probably damaging	Het
Stx19	A	G	16: 62,642,783 (GRCm39)	T200A	probably benign	Het
Tas2r116	A	G	6: 132,832,668 (GRCm39)	T90A	probably benign	Het
Tasor2	G	A	13: 3,640,069 (GRCm39)	T356I	probably benign	Het
Tbl3	A	G	17: 24,924,304 (GRCm39)		probably benign	Het
Tekt5	G	T	16: 10,213,058 (GRCm39)	P76T	probably damaging	Het
Tjp2	C	T	19: 24,074,030 (GRCm39)	E1086K	possibly damaging	Het
Topbp1	T	C	9: 103,200,770 (GRCm39)	L601P	probably damaging	Het
Trav16	T	A	14: 53,980,934 (GRCm39)	M41K	possibly damaging	Het
Trav6-5	C	A	14: 53,728,960 (GRCm39)	N72K	probably benign	Het
Trpm3	C	G	19: 22,692,659 (GRCm39)	T250S	possibly damaging	Het
Vps35	A	T	8: 85,987,891 (GRCm39)	D753E	probably benign	Het
Vstm2a	A	T	11: 16,213,061 (GRCm39)	N149Y	probably damaging	Het
Vwa2	G	T	19: 56,895,318 (GRCm39)	M497I	probably benign	Het
Zfat	C	A	15: 68,052,223 (GRCm39)	V517L	probably benign	Het
Zfp169	C	A	13: 48,643,708 (GRCm39)	R473L	possibly damaging	Het
Zfp672	T	C	11: 58,220,324 (GRCm39)		probably benign	Het
Zranb1	T	C	7: 132,574,443 (GRCm39)	V420A	probably damaging	Het

Other mutations in Lama1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Lama1	APN	17	68,122,923 (GRCm39)	missense	probably benign
IGL00336:Lama1	APN	17	68,120,943 (GRCm39)	missense	probably benign	0.07
IGL01066:Lama1	APN	17	68,050,321 (GRCm39)	missense	probably damaging	1.00
IGL01140:Lama1	APN	17	68,109,928 (GRCm39)	missense	probably benign	0.14
IGL01291:Lama1	APN	17	68,045,865 (GRCm39)	missense	probably damaging	1.00
IGL01296:Lama1	APN	17	68,052,046 (GRCm39)	missense	probably benign	0.27
IGL01317:Lama1	APN	17	68,125,696 (GRCm39)	missense	probably damaging	1.00
IGL01490:Lama1	APN	17	68,057,579 (GRCm39)	missense	possibly damaging	0.54
IGL01506:Lama1	APN	17	68,092,065 (GRCm39)	missense	probably benign	0.01
IGL01508:Lama1	APN	17	68,116,356 (GRCm39)	splice site	probably benign
IGL01522:Lama1	APN	17	68,059,769 (GRCm39)	splice site	probably benign
IGL01530:Lama1	APN	17	68,103,785 (GRCm39)	missense	probably benign	0.02
IGL01541:Lama1	APN	17	68,092,065 (GRCm39)	missense	probably benign	0.01
IGL01677:Lama1	APN	17	68,086,143 (GRCm39)	missense	probably benign	0.15
IGL01886:Lama1	APN	17	68,114,792 (GRCm39)	missense	probably benign	0.36
IGL01994:Lama1	APN	17	68,059,434 (GRCm39)	missense	probably benign	0.05
IGL02017:Lama1	APN	17	68,071,720 (GRCm39)	missense	probably benign	0.00
IGL02021:Lama1	APN	17	68,128,621 (GRCm39)	missense	probably damaging	1.00
IGL02026:Lama1	APN	17	68,116,287 (GRCm39)	missense	possibly damaging	0.82
IGL02044:Lama1	APN	17	68,118,485 (GRCm39)	missense	probably benign	0.01
IGL02120:Lama1	APN	17	68,023,784 (GRCm39)	missense	probably damaging	1.00
IGL02425:Lama1	APN	17	68,118,480 (GRCm39)	missense	probably benign	0.45
IGL02549:Lama1	APN	17	68,097,830 (GRCm39)	missense	possibly damaging	0.93
IGL02642:Lama1	APN	17	68,119,361 (GRCm39)	missense	probably benign	0.00
IGL02795:Lama1	APN	17	68,045,889 (GRCm39)	splice site	probably null
IGL02798:Lama1	APN	17	68,102,186 (GRCm39)	splice site	probably benign
IGL02863:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL02870:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL02876:Lama1	APN	17	68,057,687 (GRCm39)	critical splice donor site	probably null
IGL02885:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL02891:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL02978:Lama1	APN	17	68,093,076 (GRCm39)	nonsense	probably null
IGL03064:Lama1	APN	17	68,086,099 (GRCm39)	missense	probably benign	0.01
IGL03076:Lama1	APN	17	68,023,794 (GRCm39)	missense	possibly damaging	0.95
IGL03110:Lama1	APN	17	68,105,981 (GRCm39)	missense	probably benign	0.04
IGL03143:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL03159:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
IGL03268:Lama1	APN	17	68,111,531 (GRCm39)	missense	probably damaging	0.99
ANU05:Lama1	UTSW	17	68,045,865 (GRCm39)	missense	probably damaging	1.00
PIT4472001:Lama1	UTSW	17	68,071,699 (GRCm39)	missense
R0047:Lama1	UTSW	17	68,102,181 (GRCm39)	splice site	probably benign
R0047:Lama1	UTSW	17	68,102,181 (GRCm39)	splice site	probably benign
R0050:Lama1	UTSW	17	68,089,051 (GRCm39)	missense	possibly damaging	0.66
R0096:Lama1	UTSW	17	68,112,408 (GRCm39)	missense	probably benign	0.12
R0096:Lama1	UTSW	17	68,112,408 (GRCm39)	missense	probably benign	0.12
R0111:Lama1	UTSW	17	68,044,493 (GRCm39)	missense	probably damaging	0.98
R0116:Lama1	UTSW	17	68,083,918 (GRCm39)	missense	probably benign	0.10
R0121:Lama1	UTSW	17	68,105,508 (GRCm39)	splice site	probably benign
R0278:Lama1	UTSW	17	68,117,178 (GRCm39)	missense	probably null	0.98
R0281:Lama1	UTSW	17	68,124,564 (GRCm39)	missense	probably damaging	1.00
R0312:Lama1	UTSW	17	68,082,846 (GRCm39)	missense	possibly damaging	0.45
R0419:Lama1	UTSW	17	68,098,605 (GRCm39)	critical splice donor site	probably null
R0512:Lama1	UTSW	17	68,086,129 (GRCm39)	missense	possibly damaging	0.67
R0514:Lama1	UTSW	17	68,071,693 (GRCm39)	missense	probably benign	0.40
R0562:Lama1	UTSW	17	68,122,954 (GRCm39)	missense	probably damaging	1.00
R0632:Lama1	UTSW	17	68,059,363 (GRCm39)	splice site	probably benign
R0645:Lama1	UTSW	17	68,080,707 (GRCm39)	missense	probably benign	0.01
R0712:Lama1	UTSW	17	68,086,037 (GRCm39)	splice site	probably null
R0763:Lama1	UTSW	17	68,079,813 (GRCm39)	missense	probably damaging	0.97
R0941:Lama1	UTSW	17	68,082,860 (GRCm39)	missense	probably benign	0.10
R1025:Lama1	UTSW	17	68,059,893 (GRCm39)	missense	probably benign	0.00
R1084:Lama1	UTSW	17	68,111,464 (GRCm39)	missense	probably benign	0.12
R1103:Lama1	UTSW	17	68,097,942 (GRCm39)	missense	probably damaging	0.98
R1420:Lama1	UTSW	17	68,097,942 (GRCm39)	missense	probably damaging	0.98
R1430:Lama1	UTSW	17	68,089,150 (GRCm39)	missense	possibly damaging	0.95
R1569:Lama1	UTSW	17	68,087,613 (GRCm39)	splice site	probably null
R1575:Lama1	UTSW	17	68,117,404 (GRCm39)	missense	possibly damaging	0.96
R1613:Lama1	UTSW	17	68,114,918 (GRCm39)	missense	probably benign	0.42
R1620:Lama1	UTSW	17	68,074,028 (GRCm39)	missense	probably benign	0.01
R1629:Lama1	UTSW	17	68,112,423 (GRCm39)	missense	probably benign	0.00
R1645:Lama1	UTSW	17	68,044,677 (GRCm39)	missense	probably benign	0.14
R1652:Lama1	UTSW	17	68,114,841 (GRCm39)	missense	probably damaging	0.97
R1674:Lama1	UTSW	17	68,098,239 (GRCm39)	missense	probably benign
R1678:Lama1	UTSW	17	68,117,150 (GRCm39)	missense	possibly damaging	0.56
R1710:Lama1	UTSW	17	68,060,786 (GRCm39)	missense	probably benign	0.00
R1712:Lama1	UTSW	17	68,024,181 (GRCm39)	missense	possibly damaging	0.95
R1737:Lama1	UTSW	17	68,109,916 (GRCm39)	missense	probably benign	0.36
R1757:Lama1	UTSW	17	68,004,378 (GRCm39)	missense	unknown
R1757:Lama1	UTSW	17	68,070,831 (GRCm39)	missense	probably benign	0.40
R1813:Lama1	UTSW	17	68,098,218 (GRCm39)	missense	probably benign
R1896:Lama1	UTSW	17	68,098,218 (GRCm39)	missense	probably benign
R1945:Lama1	UTSW	17	68,052,848 (GRCm39)	missense	probably benign	0.14
R2086:Lama1	UTSW	17	68,124,618 (GRCm39)	missense	probably damaging	1.00
R2149:Lama1	UTSW	17	68,080,860 (GRCm39)	missense	possibly damaging	0.95
R2178:Lama1	UTSW	17	68,076,510 (GRCm39)	missense	probably benign	0.07
R2183:Lama1	UTSW	17	68,098,004 (GRCm39)	missense	probably damaging	0.98
R2197:Lama1	UTSW	17	68,059,936 (GRCm39)	missense	probably benign	0.02
R2213:Lama1	UTSW	17	68,084,029 (GRCm39)	nonsense	probably null
R2260:Lama1	UTSW	17	68,044,502 (GRCm39)	missense	probably damaging	0.96
R2356:Lama1	UTSW	17	68,117,109 (GRCm39)	missense	probably damaging	1.00
R2420:Lama1	UTSW	17	68,057,548 (GRCm39)	missense	probably benign	0.00
R2421:Lama1	UTSW	17	68,057,548 (GRCm39)	missense	probably benign	0.00
R2422:Lama1	UTSW	17	68,057,548 (GRCm39)	missense	probably benign	0.00
R2424:Lama1	UTSW	17	68,105,660 (GRCm39)	missense	probably benign	0.09
R2442:Lama1	UTSW	17	68,075,312 (GRCm39)	missense	probably benign	0.04
R3147:Lama1	UTSW	17	68,044,653 (GRCm39)	missense	probably damaging	0.98
R3414:Lama1	UTSW	17	68,044,598 (GRCm39)	missense	probably damaging	1.00
R3683:Lama1	UTSW	17	68,075,328 (GRCm39)	missense	probably benign	0.40
R3820:Lama1	UTSW	17	68,086,041 (GRCm39)	splice site	probably null
R3821:Lama1	UTSW	17	68,086,041 (GRCm39)	splice site	probably null
R3822:Lama1	UTSW	17	68,086,041 (GRCm39)	splice site	probably null
R4012:Lama1	UTSW	17	68,119,368 (GRCm39)	nonsense	probably null
R4113:Lama1	UTSW	17	68,071,698 (GRCm39)	missense	probably benign	0.01
R4133:Lama1	UTSW	17	68,119,481 (GRCm39)	missense	probably damaging	1.00
R4133:Lama1	UTSW	17	68,057,650 (GRCm39)	missense	probably damaging	0.98
R4259:Lama1	UTSW	17	68,059,413 (GRCm39)	missense	possibly damaging	0.95
R4278:Lama1	UTSW	17	68,098,512 (GRCm39)	missense	probably null	0.00
R4321:Lama1	UTSW	17	68,078,078 (GRCm39)	missense	probably benign	0.03
R4374:Lama1	UTSW	17	68,111,513 (GRCm39)	missense	probably benign	0.00
R4386:Lama1	UTSW	17	68,080,707 (GRCm39)	missense	probably benign	0.01
R4463:Lama1	UTSW	17	68,068,695 (GRCm39)	missense	probably damaging	1.00
R4629:Lama1	UTSW	17	68,112,355 (GRCm39)	critical splice acceptor site	probably null
R4630:Lama1	UTSW	17	68,101,295 (GRCm39)	missense	probably benign	0.00
R4633:Lama1	UTSW	17	68,105,579 (GRCm39)	missense	probably damaging	0.96
R4668:Lama1	UTSW	17	68,059,429 (GRCm39)	missense	probably benign	0.27
R4684:Lama1	UTSW	17	68,080,773 (GRCm39)	missense	possibly damaging	0.88
R4786:Lama1	UTSW	17	68,080,854 (GRCm39)	missense	possibly damaging	0.77
R4797:Lama1	UTSW	17	68,023,770 (GRCm39)	missense	probably benign	0.04
R4803:Lama1	UTSW	17	68,116,266 (GRCm39)	missense	probably damaging	1.00
R4925:Lama1	UTSW	17	68,101,309 (GRCm39)	missense	probably benign	0.02
R4939:Lama1	UTSW	17	68,044,470 (GRCm39)	missense	possibly damaging	0.91
R4952:Lama1	UTSW	17	68,074,561 (GRCm39)	critical splice donor site	probably null
R4975:Lama1	UTSW	17	68,045,829 (GRCm39)	missense	possibly damaging	0.95
R4977:Lama1	UTSW	17	68,044,677 (GRCm39)	missense	probably damaging	1.00
R5039:Lama1	UTSW	17	68,052,888 (GRCm39)	missense	possibly damaging	0.66
R5047:Lama1	UTSW	17	68,050,276 (GRCm39)	nonsense	probably null
R5195:Lama1	UTSW	17	68,071,795 (GRCm39)	missense	probably benign	0.13
R5230:Lama1	UTSW	17	68,052,078 (GRCm39)	nonsense	probably null
R5236:Lama1	UTSW	17	68,111,487 (GRCm39)	missense	probably benign	0.24
R5254:Lama1	UTSW	17	68,063,711 (GRCm39)	missense	probably benign	0.01
R5345:Lama1	UTSW	17	68,124,558 (GRCm39)	missense	probably benign
R5438:Lama1	UTSW	17	68,107,769 (GRCm39)	missense	possibly damaging	0.92
R5521:Lama1	UTSW	17	68,087,889 (GRCm39)	nonsense	probably null
R5568:Lama1	UTSW	17	68,075,293 (GRCm39)	critical splice acceptor site	probably null
R5645:Lama1	UTSW	17	68,109,943 (GRCm39)	missense	probably damaging	1.00
R5665:Lama1	UTSW	17	68,077,982 (GRCm39)	missense	probably damaging	1.00
R5727:Lama1	UTSW	17	68,122,219 (GRCm39)	missense	possibly damaging	0.81
R5757:Lama1	UTSW	17	68,045,782 (GRCm39)	missense	possibly damaging	0.59
R5795:Lama1	UTSW	17	68,103,722 (GRCm39)	missense	probably benign	0.02
R5857:Lama1	UTSW	17	68,114,838 (GRCm39)	missense	probably damaging	0.99
R5894:Lama1	UTSW	17	68,086,042 (GRCm39)	critical splice acceptor site	probably null
R5974:Lama1	UTSW	17	68,080,722 (GRCm39)	missense	probably benign	0.31
R6032:Lama1	UTSW	17	68,057,638 (GRCm39)	missense	probably benign	0.01
R6032:Lama1	UTSW	17	68,057,638 (GRCm39)	missense	probably benign	0.01
R6120:Lama1	UTSW	17	68,087,612 (GRCm39)	critical splice donor site	probably null
R6219:Lama1	UTSW	17	68,097,851 (GRCm39)	missense	probably benign	0.08
R6224:Lama1	UTSW	17	68,109,982 (GRCm39)	missense	possibly damaging	0.56
R6249:Lama1	UTSW	17	68,105,599 (GRCm39)	missense	probably benign
R6265:Lama1	UTSW	17	68,057,650 (GRCm39)	missense	probably damaging	0.98
R6276:Lama1	UTSW	17	68,091,083 (GRCm39)	splice site	probably null
R6284:Lama1	UTSW	17	68,117,091 (GRCm39)	missense	probably damaging	0.99
R6337:Lama1	UTSW	17	68,093,014 (GRCm39)	missense	probably benign	0.27
R6414:Lama1	UTSW	17	68,053,905 (GRCm39)	critical splice donor site	probably null
R6631:Lama1	UTSW	17	68,081,477 (GRCm39)	missense	probably benign	0.21
R6659:Lama1	UTSW	17	68,125,630 (GRCm39)	missense	probably damaging	1.00
R6660:Lama1	UTSW	17	68,111,495 (GRCm39)	missense	probably benign	0.05
R6677:Lama1	UTSW	17	68,102,228 (GRCm39)	missense	probably benign	0.14
R6763:Lama1	UTSW	17	68,053,868 (GRCm39)	missense	unknown
R6787:Lama1	UTSW	17	68,091,020 (GRCm39)	missense	unknown
R6831:Lama1	UTSW	17	68,063,749 (GRCm39)	missense	possibly damaging	0.89
R6855:Lama1	UTSW	17	68,089,150 (GRCm39)	missense	possibly damaging	0.95
R6910:Lama1	UTSW	17	68,098,459 (GRCm39)	missense	possibly damaging	0.60
R6934:Lama1	UTSW	17	68,081,538 (GRCm39)	missense	probably benign	0.04
R6945:Lama1	UTSW	17	68,120,861 (GRCm39)	missense
R6984:Lama1	UTSW	17	68,086,107 (GRCm39)	missense
R6989:Lama1	UTSW	17	68,060,753 (GRCm39)	missense
R6994:Lama1	UTSW	17	68,060,820 (GRCm39)	missense
R6995:Lama1	UTSW	17	68,060,820 (GRCm39)	missense
R7035:Lama1	UTSW	17	68,088,044 (GRCm39)	missense
R7133:Lama1	UTSW	17	68,089,141 (GRCm39)	missense
R7172:Lama1	UTSW	17	68,111,540 (GRCm39)	missense
R7197:Lama1	UTSW	17	68,044,700 (GRCm39)	nonsense	probably null
R7217:Lama1	UTSW	17	68,071,668 (GRCm39)	missense
R7229:Lama1	UTSW	17	68,059,441 (GRCm39)	missense
R7264:Lama1	UTSW	17	68,050,292 (GRCm39)	missense
R7311:Lama1	UTSW	17	68,074,380 (GRCm39)	missense
R7394:Lama1	UTSW	17	68,024,256 (GRCm39)	missense
R7419:Lama1	UTSW	17	68,024,169 (GRCm39)	missense
R7460:Lama1	UTSW	17	68,074,013 (GRCm39)	missense
R7492:Lama1	UTSW	17	68,124,646 (GRCm39)	missense
R7494:Lama1	UTSW	17	68,118,441 (GRCm39)	missense
R7552:Lama1	UTSW	17	68,044,662 (GRCm39)	missense
R7576:Lama1	UTSW	17	68,089,036 (GRCm39)	missense
R7583:Lama1	UTSW	17	68,068,616 (GRCm39)	missense
R7649:Lama1	UTSW	17	68,044,549 (GRCm39)	missense
R7663:Lama1	UTSW	17	68,087,875 (GRCm39)	missense
R7667:Lama1	UTSW	17	68,087,592 (GRCm39)	missense
R7688:Lama1	UTSW	17	68,068,623 (GRCm39)	missense
R7693:Lama1	UTSW	17	68,124,026 (GRCm39)	missense
R7748:Lama1	UTSW	17	68,057,585 (GRCm39)	missense
R7778:Lama1	UTSW	17	68,111,468 (GRCm39)	missense
R7824:Lama1	UTSW	17	68,111,468 (GRCm39)	missense
R7861:Lama1	UTSW	17	68,116,216 (GRCm39)	missense
R7884:Lama1	UTSW	17	68,076,430 (GRCm39)	missense
R8029:Lama1	UTSW	17	68,124,589 (GRCm39)	missense
R8078:Lama1	UTSW	17	68,098,289 (GRCm39)	missense
R8101:Lama1	UTSW	17	68,052,917 (GRCm39)	missense
R8313:Lama1	UTSW	17	68,057,515 (GRCm39)	missense
R8356:Lama1	UTSW	17	68,044,491 (GRCm39)	missense
R8366:Lama1	UTSW	17	68,125,699 (GRCm39)	missense
R8403:Lama1	UTSW	17	68,052,918 (GRCm39)	missense
R8456:Lama1	UTSW	17	68,044,491 (GRCm39)	missense
R8466:Lama1	UTSW	17	68,120,948 (GRCm39)	missense
R8678:Lama1	UTSW	17	68,124,098 (GRCm39)	missense
R8728:Lama1	UTSW	17	68,125,663 (GRCm39)	missense
R8796:Lama1	UTSW	17	68,117,146 (GRCm39)	missense
R8885:Lama1	UTSW	17	68,080,779 (GRCm39)	missense
R8893:Lama1	UTSW	17	68,112,367 (GRCm39)	missense
R8898:Lama1	UTSW	17	68,128,610 (GRCm39)	missense
R8909:Lama1	UTSW	17	68,079,736 (GRCm39)	missense
R9025:Lama1	UTSW	17	68,119,491 (GRCm39)	missense
R9045:Lama1	UTSW	17	68,060,838 (GRCm39)	missense
R9098:Lama1	UTSW	17	68,111,508 (GRCm39)	missense
R9114:Lama1	UTSW	17	68,128,669 (GRCm39)	missense
R9173:Lama1	UTSW	17	68,076,597 (GRCm39)	missense
R9190:Lama1	UTSW	17	68,111,514 (GRCm39)	missense
R9381:Lama1	UTSW	17	68,044,479 (GRCm39)	missense
R9429:Lama1	UTSW	17	68,118,449 (GRCm39)	missense
R9504:Lama1	UTSW	17	68,128,661 (GRCm39)	missense
R9558:Lama1	UTSW	17	68,124,004 (GRCm39)	missense
R9647:Lama1	UTSW	17	68,024,170 (GRCm39)	missense
R9651:Lama1	UTSW	17	68,101,215 (GRCm39)	missense
R9654:Lama1	UTSW	17	68,101,266 (GRCm39)	missense
R9710:Lama1	UTSW	17	68,129,404 (GRCm39)	missense
R9733:Lama1	UTSW	17	68,116,940 (GRCm39)	missense
RF001:Lama1	UTSW	17	68,059,897 (GRCm39)	missense
RF013:Lama1	UTSW	17	68,088,057 (GRCm39)	missense
V8831:Lama1	UTSW	17	68,059,878 (GRCm39)	missense	probably benign	0.00
X0024:Lama1	UTSW	17	68,045,883 (GRCm39)	missense	probably damaging	1.00
X0028:Lama1	UTSW	17	68,101,305 (GRCm39)	missense	probably benign	0.06
X0028:Lama1	UTSW	17	68,074,417 (GRCm39)	missense	probably benign	0.00
X0066:Lama1	UTSW	17	68,118,561 (GRCm39)	missense	probably damaging	1.00
Z1088:Lama1	UTSW	17	68,117,166 (GRCm39)	missense	probably damaging	1.00
Z1088:Lama1	UTSW	17	68,078,077 (GRCm39)	missense	probably benign	0.25
Z1088:Lama1	UTSW	17	68,059,878 (GRCm39)	missense	probably benign	0.00
Z1176:Lama1	UTSW	17	68,059,878 (GRCm39)	missense	probably benign	0.00
Z1177:Lama1	UTSW	17	68,059,878 (GRCm39)	missense	probably benign	0.00
Z1191:Lama1	UTSW	17	68,105,639 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- CTCTGATGTAGAAGGATGGGTAAC -3'
(R):5'- AGAATGTGCCCTGAAACCAAG -3'

Sequencing Primer
(F):5'- GGGCTACTGTTTATGAGTACCTAAC -3'
(R):5'- TGCCCTGAAACCAAGGTTGTC -3'

Posted On

2015-11-11