Incidental Mutation 'R4761:Gsta3'
ID 356855
Institutional Source Beutler Lab
Gene Symbol Gsta3
Ensembl Gene ENSMUSG00000025934
Gene Name glutathione S-transferase, alpha 3
Synonyms Gst2-3
MMRRC Submission 042402-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.109) question?
Stock # R4761 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 21310789-21335799 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 21330381 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 78 (T78S)
Ref Sequence ENSEMBL: ENSMUSP00000140210 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027067] [ENSMUST00000121676] [ENSMUST00000124990]
AlphaFold P30115
Predicted Effect probably benign
Transcript: ENSMUST00000027067
AA Change: T128S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000027067
Gene: ENSMUSG00000025934
AA Change: T128S

DomainStartEndE-ValueType
Pfam:GST_N 5 77 2.3e-20 PFAM
Pfam:GST_C 99 192 7.4e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000121676
AA Change: T128S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000113262
Gene: ENSMUSG00000025934
AA Change: T128S

DomainStartEndE-ValueType
Pfam:GST_N 5 77 2.4e-20 PFAM
Pfam:GST_C 99 192 1.3e-20 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000124990
AA Change: T78S

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
SMART Domains Protein: ENSMUSP00000140210
Gene: ENSMUSG00000025934
AA Change: T78S

DomainStartEndE-ValueType
Pfam:GST_N 1 27 7.7e-5 PFAM
Pfam:GST_C 49 133 3e-16 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000185842
Predicted Effect noncoding transcript
Transcript: ENSMUST00000190711
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 94.1%
Validation Efficiency 100% (69/69)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
PHENOTYPE: Targeted disruption of this gene does not alter viability, fertility or general health. However, adult homozygous mutant mice exhibit increased sensitivity to the acute cytotoxic and genotoxic effects of aflatoxin B1, a major risk factor for hepatocellular carcinoma in humans. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9630041A04Rik A G 9: 101,820,165 (GRCm39) D195G possibly damaging Het
Abcc8 G A 7: 45,762,499 (GRCm39) R1221C probably damaging Het
Adra1a T A 14: 66,964,880 (GRCm39) probably null Het
Akr1c6 A T 13: 4,497,010 (GRCm39) M98L probably benign Het
Alpk3 A G 7: 80,753,916 (GRCm39) Q1632R probably damaging Het
Arhgap24 G T 5: 102,812,080 (GRCm39) probably benign Het
Atp8b5 A G 4: 43,308,504 (GRCm39) *40W probably null Het
Bdkrb2 A T 12: 105,554,537 (GRCm39) M17L probably benign Het
C4bp T C 1: 130,581,158 (GRCm39) K117R possibly damaging Het
Cacng3 A T 7: 122,367,887 (GRCm39) T256S probably benign Het
Cep63 T C 9: 102,464,240 (GRCm39) probably benign Het
Ces2b T A 8: 105,563,193 (GRCm39) probably null Het
Col12a1 T A 9: 79,564,592 (GRCm39) D1696V probably benign Het
Cybb C G X: 9,316,989 (GRCm39) D246H probably benign Het
Dcst1 A T 3: 89,264,860 (GRCm39) M296K possibly damaging Het
Ehbp1l1 G A 19: 5,769,875 (GRCm39) P476L possibly damaging Het
Enpp1 A G 10: 24,517,849 (GRCm39) V858A possibly damaging Het
Ermp1 T A 19: 29,623,656 (GRCm39) E55D probably benign Het
Eya1 G A 1: 14,373,045 (GRCm39) L25F probably damaging Het
Foxn2 T A 17: 88,770,136 (GRCm39) probably null Het
Gabrb3 A T 7: 57,415,250 (GRCm39) T107S probably damaging Het
Gm26678 A T 3: 54,540,564 (GRCm39) noncoding transcript Het
Gm7897 G T 1: 173,657,536 (GRCm39) noncoding transcript Het
Gm9789 A T 16: 88,954,915 (GRCm39) Y8F unknown Het
H2-Ab1 A G 17: 34,486,474 (GRCm39) N178D probably damaging Het
Herc6 G A 6: 57,639,885 (GRCm39) V1000I probably benign Het
Hrnr A C 3: 93,230,062 (GRCm39) K100T probably damaging Het
Ift57 T C 16: 49,522,263 (GRCm39) F2L probably damaging Het
Iqca1l A G 5: 24,756,981 (GRCm39) V232A probably benign Het
Kmt2a C A 9: 44,760,421 (GRCm39) R509L probably damaging Het
Lefty1 T A 1: 180,765,190 (GRCm39) C253S probably benign Het
Lhx3 TCCTACGGGCCGGCCC TCC 2: 26,091,435 (GRCm39) probably null Het
Mfsd9 T C 1: 40,813,635 (GRCm39) K227E possibly damaging Het
Mrps2 C G 2: 28,359,946 (GRCm39) H268D probably benign Het
Mrps27 T A 13: 99,548,739 (GRCm39) S292T probably benign Het
Ncaph T C 2: 126,948,036 (GRCm39) D683G probably benign Het
Nlrc3 A T 16: 3,781,514 (GRCm39) S632T probably damaging Het
Nsun6 T C 2: 15,034,872 (GRCm39) T198A possibly damaging Het
Oasl2 C T 5: 115,037,836 (GRCm39) H78Y probably benign Het
Or8b54 T C 9: 38,687,133 (GRCm39) V194A probably benign Het
Pcsk5 T C 19: 17,814,512 (GRCm39) D2G possibly damaging Het
Pdgfrb C A 18: 61,212,772 (GRCm39) S892Y probably damaging Het
Phf8-ps A T 17: 33,286,172 (GRCm39) V210D probably damaging Het
Por T G 5: 135,754,784 (GRCm39) probably benign Het
Ppp2r5e A T 12: 75,640,035 (GRCm39) V22D possibly damaging Het
Prcp A G 7: 92,566,933 (GRCm39) probably null Het
Ptchd3 A T 11: 121,727,224 (GRCm39) N366I possibly damaging Het
Rprd1b A G 2: 157,889,890 (GRCm39) E4G probably damaging Het
Semp2l1 A T 1: 32,585,588 (GRCm39) S107R possibly damaging Het
Serpinb6d C A 13: 33,855,250 (GRCm39) S308Y probably damaging Het
Slc12a7 T C 13: 73,961,708 (GRCm39) V1026A probably benign Het
Slc17a7 G A 7: 44,820,408 (GRCm39) V313I probably benign Het
Slc35c1 T A 2: 92,289,168 (GRCm39) M113L probably damaging Het
Smgc A G 15: 91,729,717 (GRCm39) T178A possibly damaging Het
Spef2 A T 15: 9,653,040 (GRCm39) W914R probably damaging Het
Srfbp1 C A 18: 52,621,638 (GRCm39) P233Q probably damaging Het
Swt1 A C 1: 151,276,853 (GRCm39) C508G probably benign Het
Tex22 T C 12: 113,052,386 (GRCm39) V148A possibly damaging Het
Tle7 A T 8: 110,836,753 (GRCm39) D213V probably damaging Het
Tpd52 G A 3: 9,028,933 (GRCm39) P37L probably damaging Het
Utp25 T C 1: 192,796,230 (GRCm39) Y145C probably damaging Het
Vmn1r197 A T 13: 22,512,174 (GRCm39) M32L probably benign Het
Vmn2r100 T C 17: 19,741,630 (GRCm39) F114S possibly damaging Het
Xdh T A 17: 74,217,262 (GRCm39) I669F possibly damaging Het
Zfp940 G A 7: 29,545,578 (GRCm39) R110C probably benign Het
Other mutations in Gsta3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02274:Gsta3 APN 1 21,320,012 (GRCm39) missense possibly damaging 0.49
IGL03369:Gsta3 APN 1 21,335,173 (GRCm39) missense probably benign 0.00
R0309:Gsta3 UTSW 1 21,335,118 (GRCm39) missense possibly damaging 0.92
R1940:Gsta3 UTSW 1 21,327,601 (GRCm39) missense probably benign 0.08
R3721:Gsta3 UTSW 1 21,330,313 (GRCm39) missense probably benign 0.30
R8156:Gsta3 UTSW 1 21,330,322 (GRCm39) missense probably benign
R8433:Gsta3 UTSW 1 21,335,172 (GRCm39) missense probably benign 0.00
R8483:Gsta3 UTSW 1 21,333,063 (GRCm39) missense probably damaging 0.99
R8836:Gsta3 UTSW 1 21,330,283 (GRCm39) missense probably benign 0.35
R8897:Gsta3 UTSW 1 21,330,370 (GRCm39) missense probably benign 0.34
R9506:Gsta3 UTSW 1 21,327,586 (GRCm39) missense possibly damaging 0.53
R9516:Gsta3 UTSW 1 21,320,060 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCGCCGTTTGAGTCTTTTAAG -3'
(R):5'- TTAGTTGGCCACATGTCCC -3'

Sequencing Primer
(F):5'- GAGTCTTTTAAGTATGAAACCCCAGG -3'
(R):5'- TGGAACTCACTCTGTAGACCAGG -3'
Posted On 2015-11-11