Mutagenetix > Incidental Mutation

Incidental Mutation 'R4762:Fras1'

356949

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

042403-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4762 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

96521814-96932587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 96879477 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 2431 (H2431Q)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000036019
AA Change: H2431Q

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: H2431Q

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200077

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

100% (86/86)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600012P17Rik	T	C	1: 158,797,126 (GRCm39)		noncoding transcript	Het
2310030G06Rik	T	C	9: 50,651,967 (GRCm39)	E87G	probably damaging	Het
Aknad1	A	T	3: 108,682,547 (GRCm39)	H541L	possibly damaging	Het
Asb15	A	G	6: 24,567,236 (GRCm39)	E519G	possibly damaging	Het
Atad2	T	C	15: 57,971,758 (GRCm39)	D373G	probably benign	Het
Bbs12	G	A	3: 37,374,529 (GRCm39)	V326M	probably damaging	Het
Birc6	T	C	17: 74,936,484 (GRCm39)	I2617T	probably damaging	Het
Brca2	A	G	5: 150,454,581 (GRCm39)	T115A	probably benign	Het
Casz1	T	C	4: 149,023,438 (GRCm39)	L495P	probably damaging	Het
Ccdc30	T	A	4: 119,190,785 (GRCm39)	I481F	probably damaging	Het
Cept1	A	T	3: 106,446,677 (GRCm39)	Y56*	probably null	Het
Cfap54	A	C	10: 92,897,315 (GRCm39)		probably null	Het
Cyp2j8	G	T	4: 96,358,886 (GRCm39)	D344E	probably damaging	Het
Dido1	A	T	2: 180,331,368 (GRCm39)	W27R	probably damaging	Het
Disp3	A	G	4: 148,356,575 (GRCm39)	I95T	probably damaging	Het
Engase	G	T	11: 118,377,920 (GRCm39)	V237F	possibly damaging	Het
Ephb6	G	A	6: 41,595,094 (GRCm39)	E703K	probably damaging	Het
Fnip1	T	A	11: 54,356,997 (GRCm39)	F35L	probably damaging	Het
Fnip1	A	T	11: 54,390,352 (GRCm39)	T440S	probably benign	Het
Fzd4	A	T	7: 89,056,924 (GRCm39)	T324S	probably damaging	Het
Gm10192	C	G	4: 97,071,345 (GRCm39)	S20T	probably null	Het
Gpr84	A	T	15: 103,217,027 (GRCm39)	V350E	probably damaging	Het
Gsto2	T	C	19: 47,863,312 (GRCm39)	Y63H	probably damaging	Het
Gtpbp6	T	C	5: 110,252,186 (GRCm39)	T449A	probably damaging	Het
Herc2	G	A	7: 55,820,388 (GRCm39)	V2876I	probably benign	Het
Hnrnpa3	A	G	2: 75,492,351 (GRCm39)	I152V	possibly damaging	Het
Hpse2	T	C	19: 42,777,510 (GRCm39)	D552G	possibly damaging	Het
Itfg1	A	G	8: 86,459,070 (GRCm39)	V460A	possibly damaging	Het
Jhy	G	A	9: 40,822,494 (GRCm39)	A548V	probably benign	Het
Klhl21	T	A	4: 152,094,268 (GRCm39)	L290Q	possibly damaging	Het
Knl1	T	C	2: 118,902,417 (GRCm39)	S1373P	probably benign	Het
Kpna2	A	C	11: 106,881,085 (GRCm39)	M426R	probably benign	Het
Krt9	C	T	11: 100,081,675 (GRCm39)	V285I	probably damaging	Het
Lpar1	T	G	4: 58,437,346 (GRCm39)	H361P	possibly damaging	Het
Macf1	T	C	4: 123,349,237 (GRCm39)	T2100A	probably benign	Het
Mfsd13b	T	C	7: 120,590,549 (GRCm39)	F97L	probably damaging	Het
Mmp15	A	G	8: 96,098,958 (GRCm39)	K595R	probably benign	Het
Mrps25	C	T	6: 92,152,085 (GRCm39)	G145D	probably damaging	Het
Muc4	T	A	16: 32,574,916 (GRCm39)		probably benign	Het
Napa	A	T	7: 15,849,196 (GRCm39)	K245N	probably benign	Het
Or10p1	C	A	10: 129,444,043 (GRCm39)	M102I	probably damaging	Het
Or14c39	A	G	7: 86,344,329 (GRCm39)	T222A	probably benign	Het
Or2y1c	A	T	11: 49,361,112 (GRCm39)	I45F	probably damaging	Het
Or4g17	G	A	2: 111,210,082 (GRCm39)	V246M	probably damaging	Het
Or4k39	T	A	2: 111,239,225 (GRCm39)		noncoding transcript	Het
Or5au1	T	A	14: 52,272,921 (GRCm39)	I216F	possibly damaging	Het
Or8c20	A	G	9: 38,260,577 (GRCm39)	Y60C	probably damaging	Het
Or8k35	A	G	2: 86,424,381 (GRCm39)	S264P	possibly damaging	Het
Papss2	A	T	19: 32,616,378 (GRCm39)	T195S	probably benign	Het
Parp4	T	A	14: 56,848,267 (GRCm39)	H694Q	probably damaging	Het
Patj	C	T	4: 98,293,807 (GRCm39)	R20*	probably null	Het
Pcdhgb8	T	A	18: 37,895,419 (GRCm39)	V163E	probably damaging	Het
Pkd2l1	T	A	19: 44,144,060 (GRCm39)	T338S	probably benign	Het
Ppargc1b	A	T	18: 61,444,328 (GRCm39)	S278R	possibly damaging	Het
Ppl	T	C	16: 4,906,846 (GRCm39)	T1150A	probably benign	Het
Ralgds	A	G	2: 28,442,164 (GRCm39)	D858G	probably damaging	Het
Rassf2	G	A	2: 131,844,783 (GRCm39)		probably benign	Het
Ring1	T	C	17: 34,240,971 (GRCm39)		probably benign	Het
Rusc1	A	C	3: 88,998,949 (GRCm39)	S278A	probably benign	Het
Samd9l	T	C	6: 3,375,623 (GRCm39)	N546S	probably benign	Het
Sct	A	C	7: 140,858,954 (GRCm39)		probably benign	Het
Slc22a12	T	G	19: 6,588,474 (GRCm39)	H348P	probably benign	Het
Slc25a15	A	G	8: 22,873,248 (GRCm39)	S143P	probably damaging	Het
Slc26a11	C	A	11: 119,247,657 (GRCm39)		probably benign	Het
Slc6a20b	A	G	9: 123,427,625 (GRCm39)	M428T	probably damaging	Het
Smim17	G	A	7: 6,432,321 (GRCm39)	V88M	probably damaging	Het
Smoc1	G	T	12: 81,214,425 (GRCm39)	W269L	probably damaging	Het
Sox5	C	T	6: 143,807,109 (GRCm39)		probably null	Het
Sp100	A	G	1: 85,629,179 (GRCm39)	*483W	probably null	Het
Sptbn5	T	A	2: 119,907,703 (GRCm39)		noncoding transcript	Het
Sun5	G	A	2: 153,707,283 (GRCm39)	R132*	probably null	Het
Tedc2	A	G	17: 24,435,354 (GRCm39)	V345A	probably benign	Het
Tlr6	T	A	5: 65,111,739 (GRCm39)	R389S	probably benign	Het
Ttc17	G	T	2: 94,202,113 (GRCm39)	H396Q	probably damaging	Het
Ttn	A	G	2: 76,773,383 (GRCm39)	S2340P	probably damaging	Het
Vmn1r219	C	T	13: 23,346,999 (GRCm39)	Q63*	probably null	Het
Vmn1r224	A	G	17: 20,639,902 (GRCm39)	T160A	possibly damaging	Het
Vmn2r11	T	C	5: 109,195,436 (GRCm39)	N630S	probably damaging	Het
Zfp1005	T	A	2: 150,109,549 (GRCm39)	C80S	possibly damaging	Het
Zfp747	A	G	7: 126,973,498 (GRCm39)	V224A	possibly damaging	Het
Zfp804b	T	C	5: 6,822,250 (GRCm39)	N271S	probably benign	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96,887,217 (GRCm39)	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96,926,048 (GRCm39)	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96,907,309 (GRCm39)	splice site	probably benign
IGL00772:Fras1	APN	5	96,783,971 (GRCm39)	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96,682,712 (GRCm39)	splice site	probably benign
IGL00913:Fras1	APN	5	96,842,935 (GRCm39)	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96,929,140 (GRCm39)	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96,703,080 (GRCm39)	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96,821,557 (GRCm39)	missense	probably null	0.58
IGL01307:Fras1	APN	5	96,929,551 (GRCm39)	missense	probably benign
IGL01481:Fras1	APN	5	96,805,100 (GRCm39)	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96,887,195 (GRCm39)	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96,857,750 (GRCm39)	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96,906,007 (GRCm39)	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96,925,904 (GRCm39)	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96,735,990 (GRCm39)	missense	probably benign
IGL01869:Fras1	APN	5	96,856,642 (GRCm39)	splice site	probably benign
IGL01923:Fras1	APN	5	96,883,139 (GRCm39)	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96,887,107 (GRCm39)	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96,848,382 (GRCm39)	missense	probably benign
IGL02132:Fras1	APN	5	96,929,496 (GRCm39)	nonsense	probably null
IGL02171:Fras1	APN	5	96,883,040 (GRCm39)	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96,793,730 (GRCm39)	nonsense	probably null
IGL02277:Fras1	APN	5	96,735,977 (GRCm39)	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96,805,267 (GRCm39)	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96,917,372 (GRCm39)	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96,876,475 (GRCm39)	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96,692,883 (GRCm39)	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96,892,564 (GRCm39)	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96,839,230 (GRCm39)	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96,682,725 (GRCm39)	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96,926,034 (GRCm39)	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96,850,040 (GRCm39)	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96,857,960 (GRCm39)	missense	probably benign
IGL03078:Fras1	APN	5	96,783,994 (GRCm39)	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96,912,760 (GRCm39)	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96,874,394 (GRCm39)	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96,881,640 (GRCm39)	splice site	probably benign
IGL03189:Fras1	APN	5	96,890,930 (GRCm39)	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96,925,965 (GRCm39)	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96,855,350 (GRCm39)	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96,929,619 (GRCm39)	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96,881,803 (GRCm39)	splice site	probably benign
IGL03394:Fras1	APN	5	96,815,336 (GRCm39)	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96,876,440 (GRCm39)	missense	probably damaging	0.99
baby_ruth	UTSW	5	96,856,617 (GRCm39)	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96,888,688 (GRCm39)	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96,703,160 (GRCm39)	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96,825,175 (GRCm39)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,924,481 (GRCm39)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,924,481 (GRCm39)	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96,762,776 (GRCm39)	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96,857,936 (GRCm39)	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96,924,493 (GRCm39)	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96,884,868 (GRCm39)	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96,744,747 (GRCm39)	missense	probably benign
R0352:Fras1	UTSW	5	96,874,399 (GRCm39)	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96,910,449 (GRCm39)	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96,703,190 (GRCm39)	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96,903,368 (GRCm39)	nonsense	probably null
R0395:Fras1	UTSW	5	96,917,512 (GRCm39)	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96,839,231 (GRCm39)	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96,910,524 (GRCm39)	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96,862,202 (GRCm39)	splice site	probably null
R0456:Fras1	UTSW	5	96,702,647 (GRCm39)	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96,784,662 (GRCm39)	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96,848,347 (GRCm39)	splice site	probably benign
R0652:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96,815,246 (GRCm39)	splice site	probably benign
R0765:Fras1	UTSW	5	96,700,655 (GRCm39)	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96,916,289 (GRCm39)	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96,900,857 (GRCm39)	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96,900,857 (GRCm39)	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96,874,402 (GRCm39)	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96,862,322 (GRCm39)	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96,856,530 (GRCm39)	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96,790,488 (GRCm39)	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96,855,167 (GRCm39)	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96,855,167 (GRCm39)	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96,748,592 (GRCm39)	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96,676,445 (GRCm39)	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96,891,046 (GRCm39)	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96,784,678 (GRCm39)	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96,861,855 (GRCm39)	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96,890,921 (GRCm39)	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96,857,837 (GRCm39)	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96,861,849 (GRCm39)	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96,848,445 (GRCm39)	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96,874,472 (GRCm39)	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96,874,472 (GRCm39)	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96,746,768 (GRCm39)	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96,746,768 (GRCm39)	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96,793,732 (GRCm39)	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96,748,643 (GRCm39)	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96,700,584 (GRCm39)	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96,702,748 (GRCm39)	splice site	probably null
R1800:Fras1	UTSW	5	96,857,741 (GRCm39)	missense	probably benign
R1806:Fras1	UTSW	5	96,912,835 (GRCm39)	missense	possibly damaging	0.88
R1806:Fras1	UTSW	5	96,861,829 (GRCm39)	splice site	probably benign
R1822:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96,897,282 (GRCm39)	splice site	probably null
R1929:Fras1	UTSW	5	96,815,296 (GRCm39)	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96,860,242 (GRCm39)	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96,929,062 (GRCm39)	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96,802,164 (GRCm39)	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96,848,127 (GRCm39)	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96,881,774 (GRCm39)	missense	probably benign
R2914:Fras1	UTSW	5	96,881,774 (GRCm39)	missense	probably benign
R3054:Fras1	UTSW	5	96,912,802 (GRCm39)	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96,919,571 (GRCm39)	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96,919,571 (GRCm39)	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96,929,371 (GRCm39)	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96,793,829 (GRCm39)	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96,793,829 (GRCm39)	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96,881,791 (GRCm39)	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96,825,244 (GRCm39)	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96,918,542 (GRCm39)	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96,918,542 (GRCm39)	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96,891,017 (GRCm39)	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96,918,512 (GRCm39)	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96,918,512 (GRCm39)	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96,924,594 (GRCm39)	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96,862,235 (GRCm39)	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96,762,763 (GRCm39)	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96,848,101 (GRCm39)	missense	probably benign
R4401:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96,929,207 (GRCm39)	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96,857,754 (GRCm39)	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96,929,148 (GRCm39)	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96,924,593 (GRCm39)	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96,925,947 (GRCm39)	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96,848,427 (GRCm39)	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96,883,097 (GRCm39)	missense	probably damaging	0.96
R4735:Fras1	UTSW	5	96,736,022 (GRCm39)	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96,929,518 (GRCm39)	missense	probably benign	0.00
R4820:Fras1	UTSW	5	96,876,512 (GRCm39)	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96,692,851 (GRCm39)	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96,926,018 (GRCm39)	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96,856,617 (GRCm39)	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96,784,699 (GRCm39)	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96,924,583 (GRCm39)	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96,795,357 (GRCm39)	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96,874,439 (GRCm39)	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96,798,541 (GRCm39)	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96,792,969 (GRCm39)	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96,856,616 (GRCm39)	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96,784,032 (GRCm39)	nonsense	probably null
R5214:Fras1	UTSW	5	96,917,452 (GRCm39)	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96,916,222 (GRCm39)	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96,748,609 (GRCm39)	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96,805,109 (GRCm39)	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96,888,884 (GRCm39)	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96,883,046 (GRCm39)	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96,805,125 (GRCm39)	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96,793,019 (GRCm39)	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96,927,912 (GRCm39)	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96,676,394 (GRCm39)	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96,825,236 (GRCm39)	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96,884,880 (GRCm39)	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96,876,394 (GRCm39)	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96,929,203 (GRCm39)	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96,929,596 (GRCm39)	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96,916,240 (GRCm39)	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96,857,775 (GRCm39)	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96,879,548 (GRCm39)	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96,793,807 (GRCm39)	missense	probably benign
R6052:Fras1	UTSW	5	96,912,725 (GRCm39)	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96,857,844 (GRCm39)	missense	probably benign
R6256:Fras1	UTSW	5	96,881,702 (GRCm39)	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96,912,805 (GRCm39)	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96,907,423 (GRCm39)	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96,905,953 (GRCm39)	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96,883,061 (GRCm39)	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96,746,800 (GRCm39)	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96,746,800 (GRCm39)	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96,874,832 (GRCm39)	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96,876,410 (GRCm39)	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96,691,165 (GRCm39)	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96,830,237 (GRCm39)	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96,916,211 (GRCm39)	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96,762,732 (GRCm39)	nonsense	probably null
R7023:Fras1	UTSW	5	96,857,943 (GRCm39)	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96,856,535 (GRCm39)	missense	probably benign
R7102:Fras1	UTSW	5	96,718,900 (GRCm39)	missense	probably benign
R7120:Fras1	UTSW	5	96,900,819 (GRCm39)	nonsense	probably null
R7124:Fras1	UTSW	5	96,862,260 (GRCm39)	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96,929,143 (GRCm39)	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96,925,937 (GRCm39)	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96,903,436 (GRCm39)	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96,784,635 (GRCm39)	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96,762,771 (GRCm39)	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96,887,173 (GRCm39)	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96,784,668 (GRCm39)	missense	probably benign	0.00
R7222:Fras1	UTSW	5	96,784,045 (GRCm39)	missense	probably damaging	1.00
R7320:Fras1	UTSW	5	96,857,745 (GRCm39)	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96,884,829 (GRCm39)	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96,744,644 (GRCm39)	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96,860,309 (GRCm39)	nonsense	probably null
R7412:Fras1	UTSW	5	96,762,748 (GRCm39)	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96,821,458 (GRCm39)	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96,916,297 (GRCm39)	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96,888,713 (GRCm39)	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96,691,173 (GRCm39)	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96,832,296 (GRCm39)	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96,832,295 (GRCm39)	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96,840,483 (GRCm39)	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96,792,962 (GRCm39)	nonsense	probably null
R7722:Fras1	UTSW	5	96,917,413 (GRCm39)	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96,860,310 (GRCm39)	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96,874,754 (GRCm39)	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96,900,763 (GRCm39)	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96,887,177 (GRCm39)	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96,910,536 (GRCm39)	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96,892,633 (GRCm39)	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96,842,778 (GRCm39)	missense	probably benign
R8117:Fras1	UTSW	5	96,855,245 (GRCm39)	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96,702,714 (GRCm39)	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96,736,050 (GRCm39)	missense	probably benign
R8315:Fras1	UTSW	5	96,891,041 (GRCm39)	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96,744,711 (GRCm39)	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96,857,825 (GRCm39)	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96,839,260 (GRCm39)	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96,903,236 (GRCm39)	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96,929,207 (GRCm39)	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96,917,465 (GRCm39)	missense
R8871:Fras1	UTSW	5	96,855,257 (GRCm39)	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96,907,402 (GRCm39)	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96,929,138 (GRCm39)	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96,715,855 (GRCm39)	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96,900,774 (GRCm39)	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96,917,366 (GRCm39)	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96,860,415 (GRCm39)	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96,783,923 (GRCm39)	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96,888,609 (GRCm39)	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96,874,742 (GRCm39)	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96,883,022 (GRCm39)	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96,805,087 (GRCm39)	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96,692,904 (GRCm39)	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96,832,220 (GRCm39)	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96,910,359 (GRCm39)	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96,682,751 (GRCm39)	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96,884,813 (GRCm39)	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96,924,557 (GRCm39)	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96,929,251 (GRCm39)	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96,900,873 (GRCm39)	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96,887,124 (GRCm39)	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96,927,929 (GRCm39)	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96,884,895 (GRCm39)	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96,888,551 (GRCm39)	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96,910,387 (GRCm39)	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96,929,545 (GRCm39)	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96,717,353 (GRCm39)	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96,891,070 (GRCm39)	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1176:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1177:Fras1	UTSW	5	96,848,110 (GRCm39)	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96,839,316 (GRCm39)	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1177:Fras1	UTSW	5	96,888,842 (GRCm39)	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96,888,670 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TCTCCCTGAGGTCTAATGGCAG -3'
(R):5'- TCACCGGTCTTAGGAAAGCC -3'

Sequencing Primer
(F):5'- AGGTCTAATGGCAGTTGTGATAAG -3'
(R):5'- TCTTAGGAAAGCCAGGTCACG -3'

Posted On

2015-11-11