Mutagenetix > Incidental Mutation

Incidental Mutation 'R4749:Tnc'

357373

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

cytotactin, C130033P17Rik, TN-C, hexabrachion, tenascin-C, Hxb, TN

MMRRC Submission

041969-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4749 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63878022-63965252 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 63913876 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1312 (D1312G)

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000030056
AA Change: D1312G

PolyPhen 2 Score 0.564 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: D1312G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107372
AA Change: D1312G

PolyPhen 2 Score 0.564 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: D1312G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107377
AA Change: D1312G

PolyPhen 2 Score 0.564 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: D1312G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	A	T	19: 57,204,153 (GRCm39)	D79E	probably benign	Het
Adgra2	A	G	8: 27,604,225 (GRCm39)	K472E	probably damaging	Het
Akap9	A	G	5: 4,018,737 (GRCm39)	D1106G	probably benign	Het
Arhgap35	T	C	7: 16,232,551 (GRCm39)	E1367G	possibly damaging	Het
Arsi	A	T	18: 61,050,533 (GRCm39)	Y472F	probably benign	Het
Asxl3	A	G	18: 22,649,826 (GRCm39)	D605G	probably damaging	Het
Atp6v1e2	C	T	17: 87,252,135 (GRCm39)	D88N	probably benign	Het
BC061237	T	A	14: 44,743,469 (GRCm39)	V169E	probably damaging	Het
C1galt1	A	G	6: 7,866,379 (GRCm39)	E75G	probably benign	Het
C9	G	A	15: 6,519,311 (GRCm39)	V383I	probably benign	Het
Ccdc88a	A	G	11: 29,432,720 (GRCm39)	K222R	probably benign	Het
Ccna2	T	C	3: 36,620,391 (GRCm39)	S421G	probably benign	Het
Cflar	T	G	1: 58,779,431 (GRCm39)	V229G	possibly damaging	Het
Clcn1	A	G	6: 42,267,131 (GRCm39)		probably null	Het
Col14a1	T	A	15: 55,315,732 (GRCm39)	F1342L	unknown	Het
Colq	C	T	14: 31,251,472 (GRCm39)	R313H	possibly damaging	Het
Coro6	A	G	11: 77,359,974 (GRCm39)	E345G	probably damaging	Het
Cracd	T	C	5: 77,006,681 (GRCm39)	V1014A	unknown	Het
Cyb561	A	G	11: 105,826,708 (GRCm39)	F182L	probably benign	Het
Dap3	A	G	3: 88,833,617 (GRCm39)	S317P	probably benign	Het
Dnah9	G	A	11: 65,724,941 (GRCm39)	A4404V	probably damaging	Het
Dsg4	A	G	18: 20,579,888 (GRCm39)	E31G	possibly damaging	Het
Dsn1	G	A	2: 156,843,660 (GRCm39)	L147F	probably damaging	Het
Dysf	T	C	6: 84,043,990 (GRCm39)	V277A	probably damaging	Het
Eprs1	T	A	1: 185,128,327 (GRCm39)	I569K	probably damaging	Het
Erg	T	C	16: 95,162,029 (GRCm39)	N342S	probably damaging	Het
Fam114a1	G	A	5: 65,166,409 (GRCm39)	D247N	probably damaging	Het
Fat2	A	G	11: 55,202,294 (GRCm39)	V260A	probably benign	Het
Foxn4	T	C	5: 114,393,628 (GRCm39)	D497G	probably damaging	Het
Fsip2	A	G	2: 82,819,629 (GRCm39)	I5121V	probably benign	Het
Gcn1	T	A	5: 115,752,461 (GRCm39)	D2155E	probably benign	Het
Glra1	C	A	11: 55,427,210 (GRCm39)	D42Y	probably damaging	Het
Gpr171	A	G	3: 59,004,887 (GRCm39)	V296A	probably benign	Het
Grid1	T	A	14: 35,302,644 (GRCm39)	S970T	possibly damaging	Het
Hcn3	A	T	3: 89,057,370 (GRCm39)		probably null	Het
Helb	T	C	10: 119,920,754 (GRCm39)	D1063G	probably benign	Het
Hsd3b3	G	A	3: 98,649,931 (GRCm39)	P131S	probably damaging	Het
Hsp90b1	A	G	10: 86,537,672 (GRCm39)	V211A	probably damaging	Het
Htr2c	A	G	X: 145,976,793 (GRCm39)	T163A	probably benign	Het
Ifi208	T	A	1: 173,523,180 (GRCm39)	D483E	possibly damaging	Het
Kbtbd6	T	A	14: 79,690,727 (GRCm39)	V474E	possibly damaging	Het
Kif21b	T	A	1: 136,072,487 (GRCm39)	Y64*	probably null	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Map3k10	T	C	7: 27,357,786 (GRCm39)	D664G	possibly damaging	Het
Map3k5	C	A	10: 20,007,798 (GRCm39)	S1201Y	probably benign	Het
Mcm2	T	C	6: 88,868,973 (GRCm39)	E293G	possibly damaging	Het
Med21	T	A	6: 146,551,599 (GRCm39)		probably null	Het
Mettl18	T	C	1: 163,824,354 (GRCm39)	V225A	probably benign	Het
Mmp10	A	T	9: 7,508,169 (GRCm39)	I432F	probably damaging	Het
Muc5b	T	G	7: 141,415,185 (GRCm39)	Y2710*	probably null	Het
Neurl4	A	T	11: 69,801,894 (GRCm39)	I1282F	probably benign	Het
Nipbl	A	T	15: 8,395,313 (GRCm39)	H191Q	possibly damaging	Het
Nktr	A	G	9: 121,570,759 (GRCm39)	D167G	probably damaging	Het
Nrap	A	G	19: 56,368,669 (GRCm39)	I249T	probably damaging	Het
Nsfl1c	A	G	2: 151,351,526 (GRCm39)	T297A	probably benign	Het
Oit3	A	T	10: 59,259,904 (GRCm39)	C500S	probably damaging	Het
Or1e1	G	A	11: 73,245,322 (GRCm39)	V248M	probably damaging	Het
Or4a39	C	T	2: 89,236,599 (GRCm39)	V275I	probably benign	Het
Or4k6	A	G	14: 50,476,190 (GRCm39)	S51P	probably damaging	Het
Or5b24	C	T	19: 12,912,581 (GRCm39)	H160Y	probably benign	Het
Or5d36	T	A	2: 87,900,956 (GRCm39)	I257L	probably benign	Het
Pcnx2	T	C	8: 126,614,327 (GRCm39)	S375G	probably damaging	Het
Pgap6	T	C	17: 26,335,757 (GRCm39)	F48S	probably damaging	Het
Phf2	A	T	13: 48,975,185 (GRCm39)		probably null	Het
Piezo1	A	T	8: 123,213,678 (GRCm39)	M1739K	possibly damaging	Het
Piezo1	G	T	8: 123,224,945 (GRCm39)	Q654K	probably damaging	Het
Pml	C	T	9: 58,141,935 (GRCm39)	R299H	probably damaging	Het
Ppp3cb	A	T	14: 20,574,130 (GRCm39)	M236K	probably damaging	Het
Prkch	T	A	12: 73,739,734 (GRCm39)	C203S	probably damaging	Het
Prob1	C	T	18: 35,785,869 (GRCm39)	R795H	possibly damaging	Het
Prr22	G	C	17: 57,078,274 (GRCm39)	E142D	possibly damaging	Het
Prss56	C	A	1: 87,113,305 (GRCm39)	A211E	possibly damaging	Het
Qser1	A	C	2: 104,617,649 (GRCm39)	S1054R	probably benign	Het
Rhbdl2	T	C	4: 123,720,694 (GRCm39)		probably null	Het
Rhot2	C	A	17: 26,063,248 (GRCm39)	G19V	probably damaging	Het
Rp1l1	C	T	14: 64,267,249 (GRCm39)	T945M	probably damaging	Het
Ryr3	T	C	2: 112,794,750 (GRCm39)	T121A	possibly damaging	Het
Sdad1	C	T	5: 92,452,836 (GRCm39)	R134Q	possibly damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Sharpin	T	A	15: 76,231,767 (GRCm39)	D314V	probably damaging	Het
Slc14a2	A	T	18: 78,198,796 (GRCm39)	L778Q	probably damaging	Het
Slc29a3	A	G	10: 60,552,105 (GRCm39)	V313A	probably benign	Het
Slc4a11	C	A	2: 130,532,787 (GRCm39)	R222L	probably damaging	Het
Slc7a10	C	T	7: 34,900,187 (GRCm39)	P502S	probably damaging	Het
Sort1	T	G	3: 108,263,639 (GRCm39)	Y812*	probably null	Het
Spata31d1b	G	A	13: 59,866,172 (GRCm39)	V1107M	probably damaging	Het
Tcp10b	G	A	17: 13,289,832 (GRCm39)		probably null	Het
Tomm40l	G	A	1: 171,047,131 (GRCm39)	R296*	probably null	Het
Topors	A	T	4: 40,261,015 (GRCm39)	S756R	unknown	Het
Trim9	T	C	12: 70,295,047 (GRCm39)	N688D	probably damaging	Het
Ube2o	T	C	11: 116,432,734 (GRCm39)	D744G	probably benign	Het
Vmn1r224	A	G	17: 20,640,013 (GRCm39)	I197V	probably benign	Het
Zc3h18	A	T	8: 123,110,382 (GRCm39)	D77V	probably damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	63,935,061 (GRCm39)	splice site	probably benign
IGL00531:Tnc	APN	4	63,889,390 (GRCm39)	splice site	probably benign
IGL00674:Tnc	APN	4	63,883,844 (GRCm39)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	63,935,571 (GRCm39)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	63,918,317 (GRCm39)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	63,931,314 (GRCm39)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,901,112 (GRCm39)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	63,932,291 (GRCm39)	splice site	probably benign
IGL01411:Tnc	APN	4	63,918,959 (GRCm39)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	63,924,656 (GRCm39)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,888,645 (GRCm39)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,888,544 (GRCm39)	splice site	probably benign
IGL01669:Tnc	APN	4	63,918,938 (GRCm39)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	63,926,977 (GRCm39)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,884,909 (GRCm39)	splice site	probably benign
IGL02100:Tnc	APN	4	63,918,398 (GRCm39)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	63,933,309 (GRCm39)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,883,816 (GRCm39)	splice site	probably benign
IGL02712:Tnc	APN	4	63,893,493 (GRCm39)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	63,933,338 (GRCm39)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	63,918,344 (GRCm39)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,894,715 (GRCm39)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,889,461 (GRCm39)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	63,932,270 (GRCm39)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,885,543 (GRCm39)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	63,935,852 (GRCm39)	nonsense	probably null
tancredo	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
BB009:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
BB019:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
P0020:Tnc	UTSW	4	63,927,094 (GRCm39)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	63,935,973 (GRCm39)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,888,657 (GRCm39)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	63,935,679 (GRCm39)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	63,925,931 (GRCm39)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	63,918,396 (GRCm39)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	63,938,692 (GRCm39)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	63,926,971 (GRCm39)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,880,319 (GRCm39)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	63,938,705 (GRCm39)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	63,936,357 (GRCm39)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	63,927,096 (GRCm39)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,883,932 (GRCm39)	splice site	probably benign
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,882,991 (GRCm39)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	63,925,921 (GRCm39)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	63,924,621 (GRCm39)	missense	probably benign
R1750:Tnc	UTSW	4	63,890,972 (GRCm39)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	63,936,333 (GRCm39)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,918,168 (GRCm39)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,911,262 (GRCm39)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	63,933,201 (GRCm39)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,902,867 (GRCm39)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,882,858 (GRCm39)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,913,903 (GRCm39)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,893,475 (GRCm39)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	63,933,200 (GRCm39)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	63,938,756 (GRCm39)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	63,926,947 (GRCm39)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	63,933,188 (GRCm39)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	63,935,161 (GRCm39)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,888,588 (GRCm39)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,913,909 (GRCm39)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,913,982 (GRCm39)	missense	probably damaging	1.00
R4756:Tnc	UTSW	4	63,885,580 (GRCm39)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	63,935,857 (GRCm39)	nonsense	probably null
R4957:Tnc	UTSW	4	63,894,793 (GRCm39)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	63,924,485 (GRCm39)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,902,726 (GRCm39)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	63,924,739 (GRCm39)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	63,936,223 (GRCm39)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,893,466 (GRCm39)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,885,452 (GRCm39)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,885,489 (GRCm39)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,889,515 (GRCm39)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
R5237:Tnc	UTSW	4	63,880,333 (GRCm39)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,911,443 (GRCm39)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,882,967 (GRCm39)	nonsense	probably null
R5346:Tnc	UTSW	4	63,926,892 (GRCm39)	missense	probably benign
R5409:Tnc	UTSW	4	63,925,654 (GRCm39)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,884,773 (GRCm39)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	63,932,162 (GRCm39)	splice site	probably null
R5518:Tnc	UTSW	4	63,935,916 (GRCm39)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	63,926,946 (GRCm39)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	63,924,659 (GRCm39)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	63,925,967 (GRCm39)	splice site	probably null
R5686:Tnc	UTSW	4	63,927,032 (GRCm39)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	63,931,451 (GRCm39)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	63,936,403 (GRCm39)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,888,589 (GRCm39)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	63,927,033 (GRCm39)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	63,918,970 (GRCm39)	missense	probably damaging	1.00
R6416:Tnc	UTSW	4	63,926,053 (GRCm39)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,913,835 (GRCm39)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,900,982 (GRCm39)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,902,826 (GRCm39)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	63,931,365 (GRCm39)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,889,392 (GRCm39)	splice site	probably null
R7317:Tnc	UTSW	4	63,890,959 (GRCm39)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,889,469 (GRCm39)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,882,999 (GRCm39)	splice site	probably null
R7382:Tnc	UTSW	4	63,932,280 (GRCm39)	nonsense	probably null
R7399:Tnc	UTSW	4	63,938,894 (GRCm39)	start gained	probably benign
R7479:Tnc	UTSW	4	63,935,865 (GRCm39)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
R7947:Tnc	UTSW	4	63,935,580 (GRCm39)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	63,918,961 (GRCm39)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	63,926,983 (GRCm39)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,902,894 (GRCm39)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,894,706 (GRCm39)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	63,927,000 (GRCm39)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	63,935,716 (GRCm39)	missense	probably benign
R8340:Tnc	UTSW	4	63,926,036 (GRCm39)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,885,511 (GRCm39)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	63,935,683 (GRCm39)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,880,313 (GRCm39)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	63,924,501 (GRCm39)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,911,296 (GRCm39)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	63,927,087 (GRCm39)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	63,918,970 (GRCm39)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	63,935,331 (GRCm39)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	63,918,247 (GRCm39)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,888,622 (GRCm39)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,890,973 (GRCm39)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	63,938,686 (GRCm39)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,913,942 (GRCm39)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,884,821 (GRCm39)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	63,926,045 (GRCm39)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	63,933,186 (GRCm39)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,889,412 (GRCm39)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	63,925,600 (GRCm39)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	63,936,249 (GRCm39)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	63,925,663 (GRCm39)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,878,781 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GGAAGAAAAGCTAGTTCCCTTGC -3'
(R):5'- GCCTTCTTCAGAGGATCTCC -3'

Sequencing Primer
(F):5'- GTTCCCTTGCCATCTCAGAGAGAG -3'
(R):5'- AGGATCTCCCTCAGCTGG -3'

Posted On

2015-11-11