Mutagenetix > Incidental Mutation

Incidental Mutation 'R4743:4933407L21Rik'

358160

Institutional Source

Beutler Lab

Gene Symbol

4933407L21Rik

Ensembl Gene

ENSMUSG00000026224

Gene Name

RIKEN cDNA 4933407L21 gene

Synonyms

MMRRC Submission

041967-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R4743 (G1)

Quality Score

131

Status

Validated

Chromosome

Chromosomal Location

85856204-85859477 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to G at 85858972 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000116804 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027426] [ENSMUST00000129392]

AlphaFold

Q9D476

Predicted Effect

probably benign
Transcript: ENSMUST00000027426

Predicted Effect

probably benign
Transcript: ENSMUST00000129392

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000188949

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000190367

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahcy	T	C	2: 154,910,888 (GRCm39)	N27D	probably damaging	Het
Akap9	T	G	5: 4,011,013 (GRCm39)	L572R	probably damaging	Het
Akna	A	G	4: 63,296,850 (GRCm39)	S909P	probably damaging	Het
Atr	A	G	9: 95,744,845 (GRCm39)	E54G	probably benign	Het
Baz2b	A	G	2: 59,744,255 (GRCm39)	S1417P	probably benign	Het
Colgalt2	A	G	1: 152,276,094 (GRCm39)	Y69C	probably damaging	Het
Comp	A	G	8: 70,828,711 (GRCm39)	E181G	probably damaging	Het
Dcaf10	T	A	4: 45,370,409 (GRCm39)	S326T	probably damaging	Het
Dscam	T	C	16: 96,631,256 (GRCm39)	E253G	probably benign	Het
Dysf	C	A	6: 84,074,697 (GRCm39)	D499E	probably damaging	Het
Epha1	G	T	6: 42,349,155 (GRCm39)	T36K	probably benign	Het
Ewsr1	A	T	11: 5,033,541 (GRCm39)	D210E	unknown	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fndc1	T	A	17: 7,991,111 (GRCm39)	R862*	probably null	Het
Gbp8	T	C	5: 105,178,707 (GRCm39)	K203E	possibly damaging	Het
Gigyf2	T	A	1: 87,292,970 (GRCm39)	L79*	probably null	Het
Gm8220	T	A	14: 44,523,152 (GRCm39)		probably benign	Het
Gm9755	A	T	8: 67,966,929 (GRCm39)		noncoding transcript	Het
Il6	T	C	5: 30,223,042 (GRCm39)	Y120H	probably damaging	Het
Kcnma1	T	C	14: 23,853,270 (GRCm39)	T2A	probably damaging	Het
Mroh9	T	C	1: 162,852,061 (GRCm39)	Y876C	probably benign	Het
Plekhh2	A	G	17: 84,878,548 (GRCm39)	Y601C	probably damaging	Het
Rabgap1l	G	T	1: 160,281,353 (GRCm39)	Q623K	probably damaging	Het
Rad9a	G	A	19: 4,250,560 (GRCm39)	R85C	probably damaging	Het
Rapgef2	T	C	3: 79,080,375 (GRCm39)	Y109C	probably damaging	Het
Rhox2c	A	C	X: 36,635,351 (GRCm39)	Q4H	probably benign	Het
Rlig1	T	C	10: 100,414,243 (GRCm39)	I139V	probably benign	Het
Slain2	T	A	5: 73,114,927 (GRCm39)	L386*	probably null	Het
Slc4a7	A	G	14: 14,796,073 (GRCm38)		probably null	Het
Slco1c1	T	C	6: 141,510,242 (GRCm39)	C582R	probably damaging	Het
Sulf1	A	G	1: 12,906,517 (GRCm39)	N520D	probably benign	Het
Tbc1d7	A	G	13: 43,323,325 (GRCm39)	V22A	probably damaging	Het
Tmprss6	A	G	15: 78,327,910 (GRCm39)	V612A	probably damaging	Het
Trpv2	A	G	11: 62,483,627 (GRCm39)	E488G	probably benign	Het
Ttn	T	A	2: 76,585,168 (GRCm39)	I22042F	probably damaging	Het
Uspl1	A	G	5: 149,146,566 (GRCm39)	Y439C	probably damaging	Het
Vwf	A	G	6: 125,661,054 (GRCm39)		probably null	Het

Other mutations in 4933407L21Rik

Allele	Source	Chr	Coord	Type	Predicted Effect
R0308:4933407L21Rik	UTSW	1	85,859,007 (GRCm39)	intron	probably benign
R0458:4933407L21Rik	UTSW	1	85,856,747 (GRCm39)	missense	unknown
R3155:4933407L21Rik	UTSW	1	85,859,104 (GRCm39)	intron	probably benign
R3156:4933407L21Rik	UTSW	1	85,859,104 (GRCm39)	intron	probably benign
R3886:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3887:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3888:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3889:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R4955:4933407L21Rik	UTSW	1	85,859,008 (GRCm39)	intron	probably benign
R5745:4933407L21Rik	UTSW	1	85,858,995 (GRCm39)	splice site	probably null
R7429:4933407L21Rik	UTSW	1	85,859,028 (GRCm39)	missense	unknown
R8257:4933407L21Rik	UTSW	1	85,859,060 (GRCm39)	nonsense	probably null
R8272:4933407L21Rik	UTSW	1	85,859,118 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTAGCAATGGCACTGTCAGC -3'
(R):5'- GTCATAATGCATGCCTCTCAAGG -3'

Sequencing Primer
(F):5'- TGTCAGCCACAGAGCCCTC -3'
(R):5'- TTCCCAAGCTAGAAATGGTAGC -3'

Posted On

2015-11-11