Mutagenetix > Incidental Mutation

Incidental Mutation 'R4776:Vmn2r96'

367974

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r96

Ensembl Gene

ENSMUSG00000091679

Gene Name

vomeronasal 2, receptor 96

Synonyms

EG433070

MMRRC Submission

042413-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.157) question?

Stock #

R4776 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18793282-18818419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 18817770 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 449 (G449D)

Ref Sequence

ENSEMBL: ENSMUSP00000131564 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165692] [ENSMUST00000177244] [ENSMUST00000231261] [ENSMUST00000231286]

AlphaFold

E9PZU5

Predicted Effect

probably damaging
Transcript: ENSMUST00000165692
AA Change: G449D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000131564
Gene: ENSMUSG00000091679
AA Change: G449D

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	257	8.6e-20	PFAM
Pfam:NCD3G	317	370	3.7e-22	PFAM
Pfam:7tm_3	402	638	3.9e-53	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000177244
AA Change: G641D

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000135095
Gene: ENSMUSG00000091679
AA Change: G641D

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	245	4.2e-19	PFAM
Pfam:NCD3G	317	370	6.9e-21	PFAM
Pfam:7tm_3	400	639	3.4e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231261

Predicted Effect

probably damaging
Transcript: ENSMUST00000231286
AA Change: G641D

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 93.9%

Validation Efficiency

98% (94/96)

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610028H24Rik	A	T	10: 76,293,346 (GRCm39)	M156L	probably benign	Het
4930470P17Rik	C	T	2: 170,421,644 (GRCm39)	A79T	unknown	Het
4930522L14Rik	A	G	5: 109,884,739 (GRCm39)	I373T	probably benign	Het
Amotl1	G	A	9: 14,504,669 (GRCm39)	Q217*	probably null	Het
Ankrd28	A	T	14: 31,454,011 (GRCm39)	C254S	probably damaging	Het
Ap2a1	A	G	7: 44,550,970 (GRCm39)		probably benign	Het
Arfgef3	A	T	10: 18,529,995 (GRCm39)	S245T	probably benign	Het
Atp1b2	A	T	11: 69,492,387 (GRCm39)	D224E	probably damaging	Het
Bmal1	A	T	7: 112,884,244 (GRCm39)	K94I	probably damaging	Het
Boc	G	A	16: 44,308,084 (GRCm39)	R924W	probably damaging	Het
Btbd8	C	A	5: 107,658,317 (GRCm39)	A1117E	probably damaging	Het
Car14	C	T	3: 95,806,185 (GRCm39)	G292D	probably benign	Het
Cenpb	T	C	2: 131,020,103 (GRCm39)		probably benign	Het
Ces1b	A	T	8: 93,789,658 (GRCm39)	D423E	possibly damaging	Het
Cfap54	T	A	10: 92,808,556 (GRCm39)	N1373I	possibly damaging	Het
Chrdl2	T	C	7: 99,655,748 (GRCm39)		probably benign	Het
Cic	T	G	7: 24,982,308 (GRCm39)	S12A	possibly damaging	Het
Csmd2	A	T	4: 128,336,685 (GRCm39)	Q1421L	probably benign	Het
D630039A03Rik	T	C	4: 57,910,452 (GRCm39)	H120R	possibly damaging	Het
Dicer1	T	A	12: 104,658,705 (GRCm39)	D1779V	probably damaging	Het
Dock9	G	T	14: 121,847,509 (GRCm39)	H1016N	possibly damaging	Het
Dxo	T	C	17: 35,057,974 (GRCm39)	L352P	probably damaging	Het
Eif2b5	T	A	16: 20,318,983 (GRCm39)	F78I	probably damaging	Het
Eri2	A	G	7: 119,384,169 (GRCm39)		probably benign	Het
Fbxw7	T	G	3: 84,832,996 (GRCm39)	L13V	possibly damaging	Het
Fgf7	T	A	2: 125,877,703 (GRCm39)	C23*	probably null	Het
Fubp1	T	A	3: 151,927,705 (GRCm39)		probably null	Het
Gm2663	A	T	6: 40,972,887 (GRCm39)	I240N	probably damaging	Het
Gnb1l	C	T	16: 18,366,846 (GRCm39)	Q140*	probably null	Het
Gnptab	G	A	10: 88,272,390 (GRCm39)	R1010Q	probably damaging	Het
Gtf2h1	G	A	7: 46,472,302 (GRCm39)	W544*	probably null	Het
Gucy2c	C	T	6: 136,699,512 (GRCm39)	E586K	probably damaging	Het
Hc	T	A	2: 34,929,746 (GRCm39)	E232V	probably benign	Het
Ifi207	T	A	1: 173,557,622 (GRCm39)	D372V	unknown	Het
Igkv8-28	A	T	6: 70,121,102 (GRCm39)	V15E	probably benign	Het
Il1rap	A	G	16: 26,511,549 (GRCm39)	S198G	possibly damaging	Het
Lct	A	G	1: 128,228,124 (GRCm39)	I1123T	probably damaging	Het
Lhcgr	T	C	17: 89,050,125 (GRCm39)	E467G	probably damaging	Het
Macf1	C	T	4: 123,369,808 (GRCm39)	R86K	probably benign	Het
Maml3	T	A	3: 51,763,953 (GRCm39)	Q337L	probably benign	Het
Marchf10	T	A	11: 105,280,863 (GRCm39)	D474V	probably benign	Het
Marchf2	G	T	17: 33,928,890 (GRCm39)	T2K	probably damaging	Het
Mast1	A	G	8: 85,663,822 (GRCm39)		probably null	Het
Med12l	T	C	3: 59,140,633 (GRCm39)	I868T	probably damaging	Het
Msrb1	T	C	17: 24,959,147 (GRCm39)	S100P	probably damaging	Het
Nlrp4c	T	C	7: 6,069,125 (GRCm39)	L342P	probably benign	Het
Nrxn3	T	A	12: 90,298,730 (GRCm39)	V417E	possibly damaging	Het
Ntng1	T	A	3: 109,842,029 (GRCm39)	D248V	probably damaging	Het
Oaz3	T	C	3: 94,342,305 (GRCm39)	Q117R	probably benign	Het
Or2a56	A	G	6: 42,932,455 (GRCm39)	I8V	probably benign	Het
Or4f58	A	G	2: 111,851,276 (GRCm39)	Y308H	probably benign	Het
Or52e2	A	G	7: 102,804,621 (GRCm39)	L111P	probably benign	Het
Osbpl3	A	C	6: 50,277,953 (GRCm39)	S767A	probably benign	Het
Pafah1b1	A	T	11: 74,576,697 (GRCm39)		probably benign	Het
Pard6b	A	G	2: 167,940,708 (GRCm39)	T232A	probably damaging	Het
Paxip1	A	T	5: 27,970,204 (GRCm39)	C596S	probably damaging	Het
Pnpla6	T	C	8: 3,573,818 (GRCm39)	V422A	probably benign	Het
Psmd6	A	T	14: 14,120,932 (GRCm38)		probably benign	Het
Relch	T	A	1: 105,647,260 (GRCm39)	Y683*	probably null	Het
Rock2	T	A	12: 17,027,741 (GRCm39)	C1353S	probably damaging	Het
Rpl31-ps17	C	T	12: 54,748,397 (GRCm39)		noncoding transcript	Het
Sel1l	T	A	12: 91,780,667 (GRCm39)	H658L	probably damaging	Het
Sh3yl1	T	A	12: 30,990,313 (GRCm39)	L105Q	probably damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Sirt1	T	C	10: 63,171,501 (GRCm39)	K227E	probably benign	Het
Slc25a34	A	T	4: 141,350,899 (GRCm39)	F37I	possibly damaging	Het
Slc39a5	T	A	10: 128,232,918 (GRCm39)	I378F	probably damaging	Het
Smarcad1	A	T	6: 65,075,808 (GRCm39)	D731V	probably null	Het
Sox6	T	G	7: 115,140,905 (GRCm39)	K483N	probably damaging	Het
Sp140	T	A	1: 85,538,549 (GRCm39)	D95E	possibly damaging	Het
Srgap1	G	T	10: 121,628,256 (GRCm39)	D882E	probably benign	Het
Syne4	T	C	7: 30,016,258 (GRCm39)		probably benign	Het
Tasor2	A	G	13: 3,620,391 (GRCm39)	F2170S	probably damaging	Het
Tec	T	C	5: 72,926,119 (GRCm39)	Y289C	probably benign	Het
Tmem102	A	T	11: 69,695,628 (GRCm39)	Y115N	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trav17	T	A	14: 54,044,097 (GRCm39)	M1K	probably null	Het
Trdn	T	A	10: 33,275,078 (GRCm39)		probably null	Het
Trp53	A	T	11: 69,477,747 (GRCm39)	I8F	probably benign	Het
Ttn	T	A	2: 76,585,006 (GRCm39)	D22064V	probably damaging	Het
Ube3c	G	A	5: 29,837,836 (GRCm39)		probably null	Het
Ulk1	C	T	5: 110,936,813 (GRCm39)		probably null	Het
Upp1	T	C	11: 9,085,976 (GRCm39)	V271A	probably damaging	Het
Vmn2r4	T	C	3: 64,296,082 (GRCm39)	E901G	probably damaging	Het
Vps37b	T	C	5: 124,144,675 (GRCm39)	K165E	probably damaging	Het
Vwf	A	T	6: 125,543,268 (GRCm39)	I185F	possibly damaging	Het
Wasf2	A	G	4: 132,912,315 (GRCm39)	T56A	probably benign	Het
Zdhhc23	C	G	16: 43,793,952 (GRCm39)	D241H	possibly damaging	Het
Zfp276	T	C	8: 123,981,623 (GRCm39)	S57P	probably benign	Het
Zxdc	A	G	6: 90,347,500 (GRCm39)	H287R	probably damaging	Het

Other mutations in Vmn2r96

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00563:Vmn2r96	APN	17	18,804,077 (GRCm39)	missense	probably benign	0.00
IGL00769:Vmn2r96	APN	17	18,804,081 (GRCm39)	missense	probably benign	0.00
IGL01314:Vmn2r96	APN	17	18,803,226 (GRCm39)	missense	probably benign	0.00
IGL01983:Vmn2r96	APN	17	18,817,527 (GRCm39)	missense	probably damaging	0.99
IGL02314:Vmn2r96	APN	17	18,804,221 (GRCm39)	missense	probably benign	0.01
IGL02600:Vmn2r96	APN	17	18,817,829 (GRCm39)	missense	probably benign
IGL02672:Vmn2r96	APN	17	18,818,376 (GRCm39)	missense	probably benign	0.02
IGL02750:Vmn2r96	APN	17	18,802,851 (GRCm39)	missense	probably benign	0.01
IGL03068:Vmn2r96	APN	17	18,803,137 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r96	APN	17	18,806,634 (GRCm39)	missense	possibly damaging	0.88
R0316:Vmn2r96	UTSW	17	18,802,827 (GRCm39)	missense	probably damaging	1.00
R0502:Vmn2r96	UTSW	17	18,804,262 (GRCm39)	missense	probably benign
R0580:Vmn2r96	UTSW	17	18,802,900 (GRCm39)	missense	probably damaging	1.00
R0652:Vmn2r96	UTSW	17	18,817,830 (GRCm39)	missense	probably benign	0.05
R0789:Vmn2r96	UTSW	17	18,802,738 (GRCm39)	missense	possibly damaging	0.81
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1462:Vmn2r96	UTSW	17	18,817,660 (GRCm39)	missense	possibly damaging	0.94
R1660:Vmn2r96	UTSW	17	18,817,988 (GRCm39)	missense	probably benign	0.00
R1755:Vmn2r96	UTSW	17	18,802,915 (GRCm39)	missense	possibly damaging	0.50
R1843:Vmn2r96	UTSW	17	18,818,183 (GRCm39)	missense	probably benign	0.14
R1943:Vmn2r96	UTSW	17	18,806,664 (GRCm39)	missense	probably benign	0.09
R1993:Vmn2r96	UTSW	17	18,804,138 (GRCm39)	missense	probably damaging	1.00
R2018:Vmn2r96	UTSW	17	18,804,263 (GRCm39)	missense	probably benign
R2405:Vmn2r96	UTSW	17	18,818,102 (GRCm39)	missense	probably damaging	0.96
R3977:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R3979:Vmn2r96	UTSW	17	18,817,941 (GRCm39)	missense	probably damaging	1.00
R4059:Vmn2r96	UTSW	17	18,818,339 (GRCm39)	missense	probably benign	0.20
R4693:Vmn2r96	UTSW	17	18,803,270 (GRCm39)	missense	probably benign	0.03
R4709:Vmn2r96	UTSW	17	18,803,088 (GRCm39)	missense	probably benign	0.36
R4882:Vmn2r96	UTSW	17	18,817,866 (GRCm39)	missense	probably damaging	1.00
R4920:Vmn2r96	UTSW	17	18,802,918 (GRCm39)	missense	probably benign	0.07
R5143:Vmn2r96	UTSW	17	18,804,120 (GRCm39)	missense	possibly damaging	0.65
R5301:Vmn2r96	UTSW	17	18,817,950 (GRCm39)	missense	probably damaging	0.98
R5507:Vmn2r96	UTSW	17	18,818,091 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r96	UTSW	17	18,804,221 (GRCm39)	missense	probably benign	0.01
R6181:Vmn2r96	UTSW	17	18,804,126 (GRCm39)	missense	probably benign
R6339:Vmn2r96	UTSW	17	18,804,124 (GRCm39)	missense	possibly damaging	0.84
R6404:Vmn2r96	UTSW	17	18,817,793 (GRCm39)	missense	probably damaging	1.00
R6452:Vmn2r96	UTSW	17	18,804,117 (GRCm39)	missense	probably benign	0.07
R6749:Vmn2r96	UTSW	17	18,818,352 (GRCm39)	missense	probably damaging	0.99
R6813:Vmn2r96	UTSW	17	18,802,116 (GRCm39)	missense	probably benign	0.04
R6851:Vmn2r96	UTSW	17	18,802,800 (GRCm39)	missense	possibly damaging	0.95
R6944:Vmn2r96	UTSW	17	18,817,891 (GRCm39)	missense	probably benign	0.16
R6949:Vmn2r96	UTSW	17	18,818,100 (GRCm39)	missense	probably damaging	1.00
R6962:Vmn2r96	UTSW	17	18,818,283 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r96	UTSW	17	18,804,082 (GRCm39)	missense	probably benign
R7149:Vmn2r96	UTSW	17	18,817,989 (GRCm39)	missense	possibly damaging	0.89
R7346:Vmn2r96	UTSW	17	18,803,029 (GRCm39)	missense	probably benign	0.15
R7385:Vmn2r96	UTSW	17	18,803,302 (GRCm39)	missense	probably damaging	1.00
R7442:Vmn2r96	UTSW	17	18,793,662 (GRCm39)	missense	probably benign	0.02
R7509:Vmn2r96	UTSW	17	18,802,995 (GRCm39)	missense	probably benign	0.02
R7652:Vmn2r96	UTSW	17	18,793,832 (GRCm39)	missense	probably benign
R7659:Vmn2r96	UTSW	17	18,793,749 (GRCm39)	missense	probably benign	0.00
R7753:Vmn2r96	UTSW	17	18,806,663 (GRCm39)	missense	possibly damaging	0.46
R7855:Vmn2r96	UTSW	17	18,818,130 (GRCm39)	missense	possibly damaging	0.59
R8166:Vmn2r96	UTSW	17	18,802,744 (GRCm39)	missense	probably damaging	1.00
R8260:Vmn2r96	UTSW	17	18,804,243 (GRCm39)	missense	probably benign	0.04
R8323:Vmn2r96	UTSW	17	18,803,023 (GRCm39)	missense	probably damaging	0.99
R8787:Vmn2r96	UTSW	17	18,818,250 (GRCm39)	missense	probably damaging	1.00
R8837:Vmn2r96	UTSW	17	18,802,888 (GRCm39)	missense	probably benign
R8933:Vmn2r96	UTSW	17	18,804,241 (GRCm39)	missense	probably benign	0.11
R9306:Vmn2r96	UTSW	17	18,803,226 (GRCm39)	missense	probably benign	0.00
R9481:Vmn2r96	UTSW	17	18,793,621 (GRCm39)	start gained	probably benign
R9626:Vmn2r96	UTSW	17	18,793,758 (GRCm39)	missense	probably benign	0.14
R9629:Vmn2r96	UTSW	17	18,803,257 (GRCm39)	missense	probably benign	0.15
Z1088:Vmn2r96	UTSW	17	18,817,628 (GRCm39)	missense	possibly damaging	0.86
Z1177:Vmn2r96	UTSW	17	18,818,376 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ACCTTGGGGATGGCATTAGC -3'
(R):5'- GATGTTGCCATCCATATTCCAC -3'

Sequencing Primer
(F):5'- GATGGCATTAGCAAGCATAGC -3'
(R):5'- GATAGGAATGATGTAGTTCGGACCCC -3'

Posted On

2015-12-29