Mutagenetix > Incidental Mutation

Incidental Mutation 'R4797:Oasl1'

369127

Institutional Source

Beutler Lab

Gene Symbol

Oasl1

Ensembl Gene

ENSMUSG00000041827

Gene Name

2'-5' oligoadenylate synthetase-like 1

Synonyms

7530414C13Rik

MMRRC Submission

042421-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4797 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

115061299-115075974 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 115066217 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 112 (M112V)

Ref Sequence

ENSEMBL: ENSMUSP00000107771 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031540] [ENSMUST00000112143]

AlphaFold

Q8VI94

Predicted Effect

probably benign
Transcript: ENSMUST00000031540
AA Change: M112V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000031540
Gene: ENSMUSG00000041827
AA Change: M112V

Domain	Start	End	E-Value	Type
low complexity region	31	42	N/A	INTRINSIC
Pfam:OAS1_C	162	348	8e-76	PFAM
UBQ	350	425	1.58e0	SMART
UBQ	430	501	2.22e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112143
AA Change: M112V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000107771
Gene: ENSMUSG00000041827
AA Change: M112V

Domain	Start	End	E-Value	Type
low complexity region	31	42	N/A	INTRINSIC
Pfam:OAS1_C	163	346	1.9e-79	PFAM
UBQ	350	425	1.58e0	SMART
UBQ	430	501	2.22e-11	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127563

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140159

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152329

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155394

Meta Mutation Damage Score

0.2584

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

96% (69/72)

MGI Phenotype

PHENOTYPE: Mice with a deletion of this gene have increased expression of type I interferon and show increased resistance to viral infection. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca9	T	C	11: 110,008,945 (GRCm39)	T1195A	probably benign	Het
Apobr	G	A	7: 126,186,756 (GRCm39)	E756K	probably benign	Het
Arpc3	A	G	5: 122,542,215 (GRCm39)	E77G	possibly damaging	Het
Atp2b2	A	T	6: 113,766,847 (GRCm39)	M464K	possibly damaging	Het
Atxn7l2	A	G	3: 108,111,866 (GRCm39)	S379P	probably damaging	Het
Ccdc91	A	T	6: 147,493,641 (GRCm39)	E344D	unknown	Het
Cdc42bpg	G	A	19: 6,370,477 (GRCm39)	R1190Q	probably damaging	Het
Cdh17	C	A	4: 11,810,390 (GRCm39)	Q694K	probably benign	Het
Chordc1	G	T	9: 18,203,672 (GRCm39)		probably benign	Het
Copg1	A	G	6: 87,880,450 (GRCm39)		probably benign	Het
Dcbld1	T	C	10: 52,160,223 (GRCm39)	V37A	probably damaging	Het
Ddb2	A	G	2: 91,067,163 (GRCm39)		probably benign	Het
Dok5	A	T	2: 170,672,042 (GRCm39)	R115*	probably null	Het
Drc7	T	C	8: 95,800,925 (GRCm39)	I649T	probably damaging	Het
Efr3a	A	G	15: 65,729,437 (GRCm39)	T713A	probably damaging	Het
Epg5	G	A	18: 78,073,614 (GRCm39)	D2494N	probably benign	Het
Eps15	G	A	4: 109,223,727 (GRCm39)		probably benign	Het
Glb1l3	T	C	9: 26,739,742 (GRCm39)	D356G	probably damaging	Het
Gm1818	A	T	12: 48,602,393 (GRCm39)		noncoding transcript	Het
Gsta5	A	T	9: 78,211,679 (GRCm39)	Y147F	probably benign	Het
Hcrtr2	C	A	9: 76,161,816 (GRCm39)	M191I	probably damaging	Het
Heatr1	G	A	13: 12,426,929 (GRCm39)	E685K	probably benign	Het
Hsd3b2	A	T	3: 98,618,979 (GRCm39)	L322Q	probably damaging	Het
Hsd3b9	T	A	3: 98,363,747 (GRCm39)	R62*	probably null	Het
Htra4	T	C	8: 25,523,675 (GRCm39)	T297A	probably damaging	Het
Il22	C	A	10: 118,041,058 (GRCm39)	R55S	probably damaging	Het
Ints1	G	A	5: 139,757,631 (GRCm39)	T324M	possibly damaging	Het
Ints15	A	G	5: 143,297,504 (GRCm39)	F181S	probably benign	Het
Ints7	T	C	1: 191,329,045 (GRCm39)	V268A	probably damaging	Het
Kctd20	G	A	17: 29,185,766 (GRCm39)	V370I	probably damaging	Het
Lama1	T	A	17: 68,023,770 (GRCm39)	M55K	probably benign	Het
Larp1	C	A	11: 57,938,806 (GRCm39)	S494*	probably null	Het
Ldb3	A	T	14: 34,277,470 (GRCm39)	H262Q	possibly damaging	Het
Lepr	C	A	4: 101,637,244 (GRCm39)	T711K	possibly damaging	Het
Mon2	T	C	10: 122,852,422 (GRCm39)	I984V	probably benign	Het
Naip2	T	A	13: 100,298,243 (GRCm39)	S598C	probably damaging	Het
Or2d2b	A	T	7: 106,705,234 (GRCm39)	M278K	probably benign	Het
Or6c214	A	T	10: 129,590,390 (GRCm39)	S310T	probably benign	Het
Or7c19	G	T	8: 85,957,567 (GRCm39)	A148S	probably benign	Het
P2ry1	T	A	3: 60,910,881 (GRCm39)	S7T	probably benign	Het
Pidd1	G	T	7: 141,022,899 (GRCm39)	R98S	possibly damaging	Het
Pkd1l1	A	C	11: 8,911,340 (GRCm39)	F312L	unknown	Het
Pla2r1	A	T	2: 60,334,524 (GRCm39)	M416K	possibly damaging	Het
Pold1	T	C	7: 44,191,325 (GRCm39)	E194G	possibly damaging	Het
Poldip2	T	A	11: 78,404,813 (GRCm39)	Y77N	probably damaging	Het
Ppp2r3d	G	T	9: 101,089,179 (GRCm39)	N381K	probably benign	Het
Prrc2a	G	A	17: 35,369,018 (GRCm39)	P2006L	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rsu1	A	G	2: 13,221,537 (GRCm39)		probably benign	Het
Selenoi	T	A	5: 30,457,740 (GRCm39)	W90R	probably damaging	Het
Spag17	T	A	3: 99,891,795 (GRCm39)	D216E	possibly damaging	Het
Spata31	C	A	13: 65,070,556 (GRCm39)	Y901*	probably null	Het
Ssrp1	T	A	2: 84,876,066 (GRCm39)	Y607*	probably null	Het
Stk10	T	A	11: 32,548,471 (GRCm39)	N346K	probably benign	Het
Surf1	G	T	2: 26,806,358 (GRCm39)		probably benign	Het
Synj2	A	T	17: 6,084,163 (GRCm39)	E283V	probably damaging	Het
Tg	G	A	15: 66,629,855 (GRCm39)		probably null	Het
Traf1	A	T	2: 34,846,289 (GRCm39)	D42E	probably benign	Het
Ttn	T	C	2: 76,571,209 (GRCm39)	I26561M	probably damaging	Het
Ubp1	T	C	9: 113,785,070 (GRCm39)	Y128H	probably damaging	Het
Vmn1r216	T	A	13: 23,283,506 (GRCm39)	I63K	probably benign	Het
Vmn1r49	T	A	6: 90,049,612 (GRCm39)	H130L	probably benign	Het
Vmn2r90	C	T	17: 17,932,567 (GRCm39)	T158I	probably damaging	Het
Vps13d	T	C	4: 144,780,725 (GRCm39)	S885G	probably damaging	Het

Other mutations in Oasl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01432:Oasl1	APN	5	115,075,466 (GRCm39)	missense	probably benign	0.01
IGL02061:Oasl1	APN	5	115,061,651 (GRCm39)	missense	probably damaging	0.97
IGL02888:Oasl1	APN	5	115,075,241 (GRCm39)	missense	probably damaging	1.00
IGL03230:Oasl1	APN	5	115,075,115 (GRCm39)	missense	probably damaging	1.00
ammonite	UTSW	5	115,074,996 (GRCm39)	missense	probably damaging	1.00
dreadnaught	UTSW	5	115,074,129 (GRCm39)	critical splice donor site	probably null
nautilus	UTSW	5	115,075,242 (GRCm39)	missense	probably damaging	1.00
spirogyra	UTSW	5	115,075,465 (GRCm39)	missense	probably benign	0.00
IGL03048:Oasl1	UTSW	5	115,075,400 (GRCm39)	missense	possibly damaging	0.56
R1510:Oasl1	UTSW	5	115,066,167 (GRCm39)	missense	probably benign	0.00
R1680:Oasl1	UTSW	5	115,074,003 (GRCm39)	missense	probably damaging	1.00
R1918:Oasl1	UTSW	5	115,061,528 (GRCm39)	missense	possibly damaging	0.84
R2090:Oasl1	UTSW	5	115,073,993 (GRCm39)	missense	probably damaging	1.00
R3977:Oasl1	UTSW	5	115,070,957 (GRCm39)	missense	probably damaging	1.00
R3978:Oasl1	UTSW	5	115,070,957 (GRCm39)	missense	probably damaging	1.00
R3980:Oasl1	UTSW	5	115,070,957 (GRCm39)	missense	probably damaging	1.00
R4158:Oasl1	UTSW	5	115,075,073 (GRCm39)	missense	possibly damaging	0.77
R4159:Oasl1	UTSW	5	115,075,073 (GRCm39)	missense	possibly damaging	0.77
R4160:Oasl1	UTSW	5	115,075,073 (GRCm39)	missense	possibly damaging	0.77
R4161:Oasl1	UTSW	5	115,075,073 (GRCm39)	missense	possibly damaging	0.77
R5354:Oasl1	UTSW	5	115,075,055 (GRCm39)	missense	probably damaging	1.00
R5443:Oasl1	UTSW	5	115,074,129 (GRCm39)	critical splice donor site	probably null
R5820:Oasl1	UTSW	5	115,075,037 (GRCm39)	missense	possibly damaging	0.94
R5919:Oasl1	UTSW	5	115,066,329 (GRCm39)	missense	probably damaging	1.00
R6746:Oasl1	UTSW	5	115,075,242 (GRCm39)	missense	probably damaging	1.00
R7471:Oasl1	UTSW	5	115,073,985 (GRCm39)	missense	probably damaging	1.00
R7720:Oasl1	UTSW	5	115,067,980 (GRCm39)	missense	probably damaging	1.00
R7766:Oasl1	UTSW	5	115,075,169 (GRCm39)	missense	probably damaging	1.00
R8115:Oasl1	UTSW	5	115,074,996 (GRCm39)	missense	probably damaging	1.00
R8243:Oasl1	UTSW	5	115,066,220 (GRCm39)	missense	probably benign	0.04
R8358:Oasl1	UTSW	5	115,075,465 (GRCm39)	missense	probably benign	0.00
R9566:Oasl1	UTSW	5	115,066,331 (GRCm39)	missense	probably benign	0.02
R9695:Oasl1	UTSW	5	115,074,054 (GRCm39)	missense	probably damaging	1.00
Z1177:Oasl1	UTSW	5	115,070,804 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- GTATGAAGAGCCCCAGGTTG -3'
(R):5'- GAAAGAGGTCACAGTCTCCAG -3'

Sequencing Primer
(F):5'- CAGGTTGCTGGGCCTGTG -3'
(R):5'- CTCCAGGGGAGGCCAGG -3'

Posted On

2016-02-04