Mutagenetix > Incidental Mutation

Incidental Mutation 'R4819:Zfp935'

370071

Institutional Source

Beutler Lab

Gene Symbol

Zfp935

Ensembl Gene

ENSMUSG00000055228

Gene Name

zinc finger protein 935

Synonyms

8430426H19Rik

MMRRC Submission

042000-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R4819 (G1)

Quality Score

224

Status

Not validated

Chromosome

Chromosomal Location

62600830-62614626 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 62602231 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 323 (H323L)

Ref Sequence

ENSEMBL: ENSMUSP00000152662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000221747] [ENSMUST00000223247]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221260

Predicted Effect

probably damaging
Transcript: ENSMUST00000221747
AA Change: H323L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221951

Predicted Effect

probably benign
Transcript: ENSMUST00000223247

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.5%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,240,421 (GRCm39)	N761K	possibly damaging	Het
Adgrf3	T	A	5: 30,403,442 (GRCm39)	L444F	possibly damaging	Het
Akap8	G	A	17: 32,531,279 (GRCm39)	R378W	probably damaging	Het
Amotl2	C	T	9: 102,607,270 (GRCm39)	R693W	probably damaging	Het
As3mt	G	T	19: 46,695,968 (GRCm39)		probably benign	Het
Atp6v1e2	A	G	17: 87,251,966 (GRCm39)	V144A	probably benign	Het
Bfar	C	T	16: 13,505,331 (GRCm39)	Q114*	probably null	Het
Casd1	C	T	6: 4,621,225 (GRCm39)	A261V	probably damaging	Het
Cd177	A	T	7: 24,451,696 (GRCm39)	I440K	probably damaging	Het
Cfap54	G	T	10: 92,672,339 (GRCm39)	Y2910*	probably null	Het
Csl	A	G	10: 99,593,944 (GRCm39)	F374L	possibly damaging	Het
Dctn1	G	A	6: 83,167,501 (GRCm39)	R275H	probably damaging	Het
Derl3	A	G	10: 75,729,713 (GRCm39)		probably null	Het
Dst	A	G	1: 34,007,916 (GRCm39)	I117V	probably benign	Het
Edc3	T	A	9: 57,655,680 (GRCm39)	C477S	possibly damaging	Het
Efs	T	C	14: 55,154,610 (GRCm39)	E450G	probably damaging	Het
Fcrla	T	A	1: 170,748,508 (GRCm39)	I212F	probably damaging	Het
Fsip2	A	G	2: 82,818,786 (GRCm39)	I4840V	probably benign	Het
Gpam	T	A	19: 55,066,773 (GRCm39)	I581F	probably benign	Het
Greb1l	A	G	18: 10,458,358 (GRCm39)	D45G	probably damaging	Het
Heca	A	G	10: 17,783,820 (GRCm39)	Y478H	probably damaging	Het
Hspa9	C	T	18: 35,072,441 (GRCm39)	M561I	probably damaging	Het
Hyal6	T	A	6: 24,734,965 (GRCm39)	Y299*	probably null	Het
Iho1	A	G	9: 108,283,877 (GRCm39)	V189A	probably benign	Het
Ik	T	A	18: 36,886,310 (GRCm39)		probably null	Het
Khsrp	A	G	17: 57,330,360 (GRCm39)	S582P	possibly damaging	Het
Kif18a	A	G	2: 109,122,471 (GRCm39)	D182G	probably damaging	Het
Krt1c	T	C	15: 101,719,979 (GRCm39)	T564A	unknown	Het
Lig4	A	G	8: 10,021,885 (GRCm39)	S632P	probably benign	Het
Med1	C	T	11: 98,046,258 (GRCm39)		probably benign	Het
Mgat3	T	A	15: 80,096,550 (GRCm39)	I459N	probably damaging	Het
Mkln1	A	T	6: 31,451,421 (GRCm39)	Q454L	probably benign	Het
Mn1	A	G	5: 111,567,803 (GRCm39)	E591G	possibly damaging	Het
Myo5c	T	A	9: 75,199,484 (GRCm39)	L1364Q	probably damaging	Het
Oas1d	T	C	5: 121,053,780 (GRCm39)	V80A	probably damaging	Het
Obscn	A	T	11: 58,929,674 (GRCm39)	D5180E	probably damaging	Het
Pax6	G	A	2: 105,522,622 (GRCm39)		probably null	Het
Pcdh15	A	C	10: 74,160,221 (GRCm39)	N446T	probably damaging	Het
Pcnx2	A	T	8: 126,581,969 (GRCm39)	F922L	probably benign	Het
Ptpn4	G	T	1: 119,587,580 (GRCm39)	T921K	probably benign	Het
Selenov	A	G	7: 27,989,746 (GRCm39)		probably null	Het
Tmem100	A	G	11: 89,926,271 (GRCm39)	T33A	probably benign	Het
Tmem59	C	T	4: 107,044,878 (GRCm39)	Q66*	probably null	Het
Trav21-dv12	T	A	14: 54,114,070 (GRCm39)	Y63*	probably null	Het
Trim66	G	T	7: 109,056,793 (GRCm39)	H1121Q	probably damaging	Het
Trim80	A	G	11: 115,338,769 (GRCm39)	Y533C	probably damaging	Het
Ttc17	G	A	2: 94,194,955 (GRCm39)	P520L	probably damaging	Het
Ttn	A	T	2: 76,622,093 (GRCm39)	V15483E	probably damaging	Het
Vinac1	G	T	2: 128,882,721 (GRCm39)	N98K	probably damaging	Het
Zdhhc1	CGGGGG	CGGGGGG	8: 106,210,376 (GRCm39)		probably null	Het
Zfhx4	C	A	3: 5,468,974 (GRCm39)	T3069K	probably benign	Het
Zfp281	A	G	1: 136,553,448 (GRCm39)	H142R	probably benign	Het
Zfp462	G	T	4: 55,060,044 (GRCm39)	R1190L	probably damaging	Het

Other mutations in Zfp935

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00791:Zfp935	APN	13	62,602,278 (GRCm39)	missense	probably benign	0.33
IGL02169:Zfp935	APN	13	62,604,745 (GRCm39)	critical splice donor site	probably null
IGL02570:Zfp935	APN	13	62,604,792 (GRCm39)	missense	probably damaging	1.00
IGL02756:Zfp935	APN	13	62,602,701 (GRCm39)	nonsense	probably null
IGL03338:Zfp935	APN	13	62,602,247 (GRCm39)	missense	probably benign	0.11
R1252:Zfp935	UTSW	13	62,602,355 (GRCm39)	missense	probably damaging	1.00
R1503:Zfp935	UTSW	13	62,602,951 (GRCm39)	missense	possibly damaging	0.65
R2425:Zfp935	UTSW	13	62,602,922 (GRCm39)	missense	probably benign	0.02
R3720:Zfp935	UTSW	13	62,602,846 (GRCm39)	nonsense	probably null
R5391:Zfp935	UTSW	13	62,602,632 (GRCm39)	nonsense	probably null
R6253:Zfp935	UTSW	13	62,602,685 (GRCm39)	missense	probably benign	0.02
R6742:Zfp935	UTSW	13	62,602,293 (GRCm39)	missense	probably damaging	1.00
R9447:Zfp935	UTSW	13	62,602,842 (GRCm39)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- TAACTGTGTTGTGAAAAGGCTG -3'
(R):5'- TCCCAATACGTTCATCTCCAAAT -3'

Sequencing Primer
(F):5'- TGTGAAAAGGCTGTCACATTG -3'
(R):5'- TACTGGAGAGAAACCCTTCAAATGTG -3'

Posted On

2016-02-04