Mutagenetix > Incidental Mutation

Incidental Mutation 'R4802:Polr1a'

370292

Institutional Source

Beutler Lab

Gene Symbol

Polr1a

Ensembl Gene

ENSMUSG00000049553

Gene Name

polymerase (RNA) I polypeptide A

Synonyms

2900087K15Rik, 3010014K16Rik, 194kDa, mRPA1, Rpo1-4, RPA194

MMRRC Submission

042424-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4802 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

71886037-71956419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 71953054 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 1541 (V1541I)

Ref Sequence

ENSEMBL: ENSMUSP00000060858 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055296]

AlphaFold

O35134

Predicted Effect

probably benign
Transcript: ENSMUST00000055296
AA Change: V1541I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000060858
Gene: ENSMUSG00000049553
AA Change: V1541I

Domain	Start	End	E-Value	Type
RPOLA_N	302	649	8.97e-137	SMART
Pfam:RNA_pol_Rpb1_4	846	958	1.3e-26	PFAM
Pfam:RNA_pol_Rpb1_5	965	1669	7e-103	PFAM
low complexity region	1698	1708	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000205517
AA Change: V566I

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

97% (93/96)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]

Allele List at MGI

All alleles(18) : Gene trapped(18)

Other mutations in this stock

Total: 167 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	A	G	10: 28,859,922 (GRCm39)		probably null	Het
4932416K20Rik	T	A	8: 105,523,664 (GRCm39)		noncoding transcript	Het
Aadacl3	A	G	4: 144,182,802 (GRCm39)	I222T	probably damaging	Het
Abca13	G	T	11: 9,472,341 (GRCm39)	G4249V	possibly damaging	Het
Abcb10	A	G	8: 124,693,266 (GRCm39)	V346A	probably benign	Het
Abcc2	A	T	19: 43,807,800 (GRCm39)	I814F	probably damaging	Het
Alkbh2	C	T	5: 114,262,287 (GRCm39)	E148K	probably damaging	Het
Ankrd28	A	C	14: 31,458,787 (GRCm39)	D335E	probably damaging	Het
Ankrd44	T	C	1: 54,801,475 (GRCm39)	H284R	probably damaging	Het
Arfgef3	G	T	10: 18,467,654 (GRCm39)	Q1849K	probably benign	Het
Bach1	T	A	16: 87,519,340 (GRCm39)	D543E	probably damaging	Het
Bahcc1	G	A	11: 120,173,051 (GRCm39)	V1558I	probably benign	Het
Bbs5	T	A	2: 69,485,958 (GRCm39)	W168R	probably damaging	Het
Bcar3	A	T	3: 122,323,243 (GRCm39)	D766V	probably benign	Het
C1ra	A	G	6: 124,490,727 (GRCm39)	D40G	probably benign	Het
Cbr4	T	C	8: 61,943,113 (GRCm39)		probably benign	Het
Ccdc138	G	A	10: 58,409,465 (GRCm39)	C598Y	probably damaging	Het
Cd200r3	T	A	16: 44,778,188 (GRCm39)	N197K	possibly damaging	Het
Cenpf	T	A	1: 189,383,417 (GRCm39)	E2634D	probably damaging	Het
Cisd2	T	C	3: 135,116,902 (GRCm39)	K63R	probably damaging	Het
Clca3a2	A	T	3: 144,513,112 (GRCm39)	S478T	possibly damaging	Het
Clptm1l	T	C	13: 73,755,981 (GRCm39)	M199T	possibly damaging	Het
Cntnap4	T	A	8: 113,500,222 (GRCm39)	S505T	possibly damaging	Het
Cr2	C	T	1: 194,845,619 (GRCm39)	G112D	probably damaging	Het
Crb2	T	A	2: 37,683,768 (GRCm39)	I1090N	probably benign	Het
Csmd3	G	T	15: 47,484,688 (GRCm39)	P3057Q	probably damaging	Het
Ctso	G	A	3: 81,861,547 (GRCm39)	V307I	probably damaging	Het
Cyp3a41b	T	A	5: 145,510,461 (GRCm39)	T138S	probably benign	Het
Dctn3	G	T	4: 41,719,904 (GRCm39)	Y67*	probably null	Het
Dennd4c	C	A	4: 86,738,121 (GRCm39)	Y918*	probably null	Het
Dlg5	C	T	14: 24,204,757 (GRCm39)	G1262D	probably damaging	Het
Dnaaf1	G	A	8: 120,304,100 (GRCm39)	G46D	probably benign	Het
Dnah6	A	G	6: 73,066,681 (GRCm39)	V2563A	probably damaging	Het
Dnajc13	A	G	9: 104,052,926 (GRCm39)	Y1679H	probably benign	Het
Eif2ak3	T	C	6: 70,864,877 (GRCm39)	Y578H	probably benign	Het
Endod1	C	T	9: 14,268,319 (GRCm39)	V389M	probably benign	Het
Entpd2	T	G	2: 25,289,776 (GRCm39)		probably null	Het
Ephb4	T	C	5: 137,363,768 (GRCm39)	L582P	probably damaging	Het
Eps15	T	C	4: 109,181,414 (GRCm39)	L316S	possibly damaging	Het
Erbb4	G	T	1: 68,369,405 (GRCm39)	T412K	probably damaging	Het
Fam117a	T	A	11: 95,254,896 (GRCm39)	F90I	probably damaging	Het
Fam187b	T	G	7: 30,676,515 (GRCm39)	V8G	possibly damaging	Het
Far1	T	A	7: 113,138,660 (GRCm39)	I59N	possibly damaging	Het
Fbxo34	T	A	14: 47,768,326 (GRCm39)	L562Q	probably damaging	Het
Frem1	T	A	4: 82,834,865 (GRCm39)		probably benign	Het
Gfra3	G	T	18: 34,853,245 (GRCm39)	P10Q	probably damaging	Het
Gm10619	T	A	7: 73,459,773 (GRCm39)		noncoding transcript	Het
Gm19965	T	A	1: 116,749,626 (GRCm39)	Y436N	probably benign	Het
Gm28042	T	C	2: 119,872,535 (GRCm39)		probably benign	Het
Gm4953	A	T	1: 158,995,988 (GRCm39)		noncoding transcript	Het
Gm5799	A	T	14: 43,782,005 (GRCm39)	H59L	probably damaging	Het
Gmppb	T	A	9: 107,927,416 (GRCm39)	V121E	probably benign	Het
Ighv7-4	G	C	12: 114,186,899 (GRCm39)		probably benign	Het
Ipo4	A	G	14: 55,868,671 (GRCm39)	S446P	probably damaging	Het
Itpr2	T	C	6: 146,272,829 (GRCm39)	T855A	probably damaging	Het
Jaml	T	C	9: 45,012,362 (GRCm39)	I283T	possibly damaging	Het
Klhl32	T	C	4: 24,649,698 (GRCm39)	Y399C	possibly damaging	Het
Lratd2	C	A	15: 60,695,793 (GRCm39)		probably benign	Het
Lrriq1	G	A	10: 103,057,179 (GRCm39)	T207I	probably benign	Het
Lrriq3	A	T	3: 154,893,607 (GRCm39)	H436L	probably benign	Het
Mad2l1bp	T	C	17: 46,459,189 (GRCm39)	K114E	possibly damaging	Het
Mamstr	T	C	7: 45,291,842 (GRCm39)	V64A	possibly damaging	Het
Map4	T	A	9: 109,864,325 (GRCm39)	S517T	probably benign	Het
Matn1	A	T	4: 130,677,336 (GRCm39)	I182F	possibly damaging	Het
Mcm3	A	G	1: 20,880,380 (GRCm39)	I484T	probably damaging	Het
Med13	T	A	11: 86,169,599 (GRCm39)	I1922F	probably damaging	Het
Metap2	A	G	10: 93,704,757 (GRCm39)	V137A	probably damaging	Het
Mex3d	A	G	10: 80,222,788 (GRCm39)	V156A	possibly damaging	Het
Mfsd6	G	A	1: 52,748,755 (GRCm39)	P37S	probably benign	Het
Mmut	A	G	17: 41,248,242 (GRCm39)	T90A	probably benign	Het
Mrgprx1	A	C	7: 47,670,959 (GRCm39)	S263A	possibly damaging	Het
Mrtfa	T	C	15: 80,989,000 (GRCm39)	E7G	probably benign	Het
Msantd5f6	C	T	4: 73,319,504 (GRCm39)	W80*	probably null	Het
Msl1	C	T	11: 98,694,795 (GRCm39)	R505*	probably null	Het
Mta2	T	C	19: 8,923,215 (GRCm39)	S96P	probably damaging	Het
Mtr	A	T	13: 12,210,137 (GRCm39)	N986K	probably benign	Het
Mutyh	C	T	4: 116,674,226 (GRCm39)	T259I	probably benign	Het
Myof	T	C	19: 37,934,186 (GRCm39)	T908A	probably benign	Het
Nav2	A	T	7: 49,195,600 (GRCm39)	D992V	possibly damaging	Het
Neb	T	C	2: 52,090,715 (GRCm39)	T1352A	possibly damaging	Het
Nfix	CAAAAA	CAAAA	8: 85,442,876 (GRCm39)		probably null	Het
Nudt19	T	C	7: 35,255,564 (GRCm39)		probably benign	Het
Nudt6	G	A	3: 37,459,503 (GRCm39)	R161C	probably benign	Het
Olr1	A	G	6: 129,465,053 (GRCm39)	F141S	possibly damaging	Het
Oprl1	G	T	2: 181,361,046 (GRCm39)	M340I	probably benign	Het
Or10ag57	T	A	2: 87,218,553 (GRCm39)	V168E	probably benign	Het
Or10d1	T	G	9: 39,484,154 (GRCm39)	M134L	probably benign	Het
Or11g2	A	G	14: 50,856,479 (GRCm39)	T267A	probably benign	Het
Or14j1	T	C	17: 38,146,240 (GRCm39)	Y117H	probably damaging	Het
Or1e17	T	A	11: 73,831,696 (GRCm39)	I208K	possibly damaging	Het
Or2ak6	T	C	11: 58,592,617 (GRCm39)	V30A	probably benign	Het
Or2r2	A	T	6: 42,463,613 (GRCm39)	N171K	probably benign	Het
Or4f53	T	A	2: 111,087,497 (GRCm39)	F12L	probably damaging	Het
Or5d43	T	A	2: 88,105,223 (GRCm39)	M57L	probably damaging	Het
Or5g26	T	A	2: 85,494,622 (GRCm39)	D52V	probably damaging	Het
Or9s15	T	A	1: 92,524,720 (GRCm39)	C160S	probably benign	Het
Otogl	A	C	10: 107,737,197 (GRCm39)	C72W	probably damaging	Het
Pcdha11	T	A	18: 37,138,518 (GRCm39)	I49N	probably damaging	Het
Pcdha4	T	A	18: 37,087,008 (GRCm39)	L397*	probably null	Het
Pcdhb14	A	G	18: 37,581,331 (GRCm39)	S146G	probably benign	Het
Pclo	T	A	5: 14,725,829 (GRCm39)	H1562Q	unknown	Het
Pcsk9	T	C	4: 106,304,766 (GRCm39)	E434G	probably benign	Het
Phc2	A	G	4: 128,645,391 (GRCm39)	K833E	probably damaging	Het
Pja2	A	T	17: 64,599,857 (GRCm39)	S480R	probably damaging	Het
Pkd1	G	A	17: 24,797,070 (GRCm39)	G2493D	probably damaging	Het
Plk5	G	A	10: 80,195,138 (GRCm39)	V179M	possibly damaging	Het
Ppard	C	G	17: 28,505,348 (GRCm39)	R12G	unknown	Het
Ppp1r14a	A	G	7: 28,990,951 (GRCm39)	D73G	probably damaging	Het
Psd3	C	T	8: 68,573,800 (GRCm39)	R127H	probably benign	Het
Pten	G	T	19: 32,735,903 (GRCm39)	G20V	possibly damaging	Het
Ptprf	G	A	4: 118,067,526 (GRCm39)		probably benign	Het
Rad54l	T	C	4: 115,980,121 (GRCm39)	D21G	probably null	Het
Rgs14	T	C	13: 55,528,770 (GRCm39)	Y304H	probably damaging	Het
Rgs9	T	C	11: 109,131,694 (GRCm39)	K346R	probably damaging	Het
Rnf169	C	G	7: 99,575,653 (GRCm39)	G314A	probably damaging	Het
Rpgrip1l	T	A	8: 91,996,805 (GRCm39)	T692S	probably damaging	Het
Rtf1	T	C	2: 119,505,709 (GRCm39)	V54A	possibly damaging	Het
Rtn4	T	C	11: 29,658,660 (GRCm39)	V938A	probably benign	Het
Ryr2	T	C	13: 11,723,113 (GRCm39)	T2509A	probably damaging	Het
Ryr2	T	A	13: 11,702,818 (GRCm39)	D2890V	probably damaging	Het
Scel	A	C	14: 103,820,536 (GRCm39)	T348P	probably benign	Het
Scgb1b2	G	T	7: 30,990,998 (GRCm39)	L37I	possibly damaging	Het
Sdf4	A	G	4: 156,085,178 (GRCm39)	H171R	possibly damaging	Het
Sec31a	A	G	5: 100,541,222 (GRCm39)	V295A	probably damaging	Het
Semp2l2a	T	A	8: 13,886,970 (GRCm39)	I374F	possibly damaging	Het
Setx	T	A	2: 29,036,385 (GRCm39)	S957T	probably benign	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Six5	A	G	7: 18,830,894 (GRCm39)	N507S	probably benign	Het
Slc12a7	T	C	13: 73,912,011 (GRCm39)		probably null	Het
Slc1a7	T	A	4: 107,850,237 (GRCm39)	V116E	probably damaging	Het
Slc22a1	A	G	17: 12,894,422 (GRCm39)	L42P	probably damaging	Het
Slc25a31	A	T	3: 40,675,975 (GRCm39)	I174F	probably damaging	Het
Slc31a2	A	T	4: 62,210,869 (GRCm39)	M3L	probably damaging	Het
Slco1a4	T	A	6: 141,791,223 (GRCm39)		probably benign	Het
Smcr8	C	T	11: 60,669,436 (GRCm39)		probably null	Het
Smg5	G	T	3: 88,262,999 (GRCm39)	E801*	probably null	Het
Smgc	C	A	15: 91,738,819 (GRCm39)	H492Q	probably benign	Het
Smyd4	G	T	11: 75,294,010 (GRCm39)	G694V	probably damaging	Het
Sorcs3	A	G	19: 48,387,183 (GRCm39)	T223A	possibly damaging	Het
Stab1	G	T	14: 30,863,328 (GRCm39)	C2119*	probably null	Het
Taar9	A	G	10: 23,984,741 (GRCm39)	I231T	probably damaging	Het
Tacr2	A	G	10: 62,097,327 (GRCm39)	Y269C	probably damaging	Het
Taf3	T	G	2: 9,955,934 (GRCm39)	K744N	possibly damaging	Het
Tarbp1	C	G	8: 127,201,628 (GRCm39)	E59D	possibly damaging	Het
Tcstv1b	T	A	13: 120,634,758 (GRCm39)	S13R	probably benign	Het
Tenm4	T	A	7: 96,555,452 (GRCm39)	V2682E	probably damaging	Het
Tet1	A	C	10: 62,658,442 (GRCm39)	L1468R	probably damaging	Het
Tgfb2	A	T	1: 186,361,110 (GRCm39)	Y380*	probably null	Het
Tgm5	T	G	2: 120,882,953 (GRCm39)	K435Q	probably damaging	Het
Themis	A	G	10: 28,637,507 (GRCm39)	T204A	probably benign	Het
Tm4sf1	T	C	3: 57,202,100 (GRCm39)	Y37C	probably damaging	Het
Tnn	T	C	1: 159,972,603 (GRCm39)	N333S	possibly damaging	Het
Tppp2	A	G	14: 52,156,805 (GRCm39)	N61D	probably benign	Het
Treml2	A	G	17: 48,616,187 (GRCm39)	T276A	probably benign	Het
Trit1	T	C	4: 122,910,431 (GRCm39)	V10A	probably benign	Het
Ttn	T	A	2: 76,794,990 (GRCm39)		probably benign	Het
Uba1y	T	G	Y: 825,890 (GRCm39)		probably null	Het
Vcam1	C	G	3: 115,909,584 (GRCm39)	G581A	probably damaging	Het
Vmn1r16	G	A	6: 57,300,175 (GRCm39)	T149I	probably benign	Het
Vmn1r209	T	C	13: 22,989,826 (GRCm39)	D288G	probably damaging	Het
Vmn1r78	T	A	7: 11,886,891 (GRCm39)	Y167*	probably null	Het
Vmn2r103	T	A	17: 20,015,338 (GRCm39)	S493T	probably benign	Het
Vmn2r105	T	A	17: 20,447,556 (GRCm39)	M423L	probably benign	Het
Vps13c	T	C	9: 67,871,564 (GRCm39)	F3244L	probably damaging	Het
Zfp119b	A	T	17: 56,246,642 (GRCm39)	D149E	probably damaging	Het
Zfp345	T	C	2: 150,315,228 (GRCm39)	Y103C	possibly damaging	Het
Zmym6	C	T	4: 127,017,009 (GRCm39)	T930I	probably benign	Het

Other mutations in Polr1a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01363:Polr1a	APN	6	71,925,470 (GRCm39)	missense	probably benign	0.32
IGL01834:Polr1a	APN	6	71,925,446 (GRCm39)	missense	probably benign
IGL01902:Polr1a	APN	6	71,940,732 (GRCm39)	missense	probably damaging	1.00
IGL02101:Polr1a	APN	6	71,927,786 (GRCm39)	missense	probably benign	0.00
IGL02325:Polr1a	APN	6	71,897,641 (GRCm39)	missense	probably benign	0.38
IGL02398:Polr1a	APN	6	71,913,540 (GRCm39)	splice site	probably benign
IGL02528:Polr1a	APN	6	71,941,701 (GRCm39)	missense	probably benign
IGL02555:Polr1a	APN	6	71,897,441 (GRCm39)	missense	probably damaging	0.98
IGL02613:Polr1a	APN	6	71,944,304 (GRCm39)	missense	probably damaging	1.00
IGL02693:Polr1a	APN	6	71,940,830 (GRCm39)	splice site	probably benign
IGL02892:Polr1a	APN	6	71,908,680 (GRCm39)	missense	possibly damaging	0.70
IGL03059:Polr1a	APN	6	71,913,496 (GRCm39)	missense	probably benign
IGL03174:Polr1a	APN	6	71,954,331 (GRCm39)	missense	possibly damaging	0.82
D4043:Polr1a	UTSW	6	71,918,401 (GRCm39)	missense	possibly damaging	0.92
R0092:Polr1a	UTSW	6	71,944,439 (GRCm39)	splice site	probably benign
R0217:Polr1a	UTSW	6	71,940,687 (GRCm39)	missense	probably benign	0.19
R0267:Polr1a	UTSW	6	71,951,123 (GRCm39)	missense	probably damaging	0.99
R0329:Polr1a	UTSW	6	71,943,400 (GRCm39)	missense	possibly damaging	0.96
R0330:Polr1a	UTSW	6	71,943,400 (GRCm39)	missense	possibly damaging	0.96
R0352:Polr1a	UTSW	6	71,897,747 (GRCm39)	splice site	probably benign
R0411:Polr1a	UTSW	6	71,955,405 (GRCm39)	missense	possibly damaging	0.95
R0446:Polr1a	UTSW	6	71,927,648 (GRCm39)	critical splice donor site	probably null
R0846:Polr1a	UTSW	6	71,901,627 (GRCm39)	missense	probably damaging	1.00
R1035:Polr1a	UTSW	6	71,944,900 (GRCm39)	missense	probably benign
R1294:Polr1a	UTSW	6	71,889,886 (GRCm39)	missense	probably damaging	0.99
R1460:Polr1a	UTSW	6	71,918,368 (GRCm39)	missense	probably damaging	0.99
R1657:Polr1a	UTSW	6	71,918,519 (GRCm39)	missense	probably damaging	1.00
R1846:Polr1a	UTSW	6	71,953,172 (GRCm39)	missense	probably damaging	0.98
R1862:Polr1a	UTSW	6	71,886,187 (GRCm39)	missense	probably damaging	0.96
R1865:Polr1a	UTSW	6	71,943,508 (GRCm39)	missense	probably damaging	1.00
R1903:Polr1a	UTSW	6	71,944,898 (GRCm39)	missense	probably benign	0.02
R1937:Polr1a	UTSW	6	71,913,536 (GRCm39)	critical splice donor site	probably null
R2063:Polr1a	UTSW	6	71,913,269 (GRCm39)	splice site	probably null
R2071:Polr1a	UTSW	6	71,953,058 (GRCm39)	missense	possibly damaging	0.64
R2084:Polr1a	UTSW	6	71,927,793 (GRCm39)	missense	possibly damaging	0.69
R2377:Polr1a	UTSW	6	71,949,810 (GRCm39)	critical splice donor site	probably null
R2410:Polr1a	UTSW	6	71,951,866 (GRCm39)	missense	probably benign
R3001:Polr1a	UTSW	6	71,890,000 (GRCm39)	missense	probably benign	0.01
R3001:Polr1a	UTSW	6	71,942,628 (GRCm39)	missense	probably benign	0.02
R3002:Polr1a	UTSW	6	71,942,628 (GRCm39)	missense	probably benign	0.02
R3002:Polr1a	UTSW	6	71,890,000 (GRCm39)	missense	probably benign	0.01
R3924:Polr1a	UTSW	6	71,906,434 (GRCm39)	missense	probably benign	0.00
R4105:Polr1a	UTSW	6	71,953,175 (GRCm39)	missense	probably damaging	0.98
R4125:Polr1a	UTSW	6	71,942,690 (GRCm39)	missense	probably benign	0.00
R4271:Polr1a	UTSW	6	71,930,006 (GRCm39)	missense	probably benign	0.02
R4440:Polr1a	UTSW	6	71,927,832 (GRCm39)	missense	probably damaging	0.98
R4667:Polr1a	UTSW	6	71,894,805 (GRCm39)	missense	probably benign	0.30
R4769:Polr1a	UTSW	6	71,927,852 (GRCm39)	missense	probably benign	0.01
R4801:Polr1a	UTSW	6	71,953,054 (GRCm39)	missense	probably benign	0.00
R4828:Polr1a	UTSW	6	71,943,385 (GRCm39)	missense	possibly damaging	0.93
R4911:Polr1a	UTSW	6	71,886,213 (GRCm39)	missense	possibly damaging	0.67
R5071:Polr1a	UTSW	6	71,908,693 (GRCm39)	missense	possibly damaging	0.71
R5165:Polr1a	UTSW	6	71,944,909 (GRCm39)	missense	probably damaging	1.00
R5223:Polr1a	UTSW	6	71,944,891 (GRCm39)	missense	possibly damaging	0.73
R5239:Polr1a	UTSW	6	71,890,021 (GRCm39)	missense	probably damaging	1.00
R5546:Polr1a	UTSW	6	71,906,350 (GRCm39)	missense	possibly damaging	0.64
R5599:Polr1a	UTSW	6	71,944,346 (GRCm39)	missense	possibly damaging	0.95
R5696:Polr1a	UTSW	6	71,906,410 (GRCm39)	missense	probably benign	0.05
R5850:Polr1a	UTSW	6	71,903,667 (GRCm39)	missense	probably benign	0.00
R6274:Polr1a	UTSW	6	71,931,874 (GRCm39)	splice site	probably null
R6526:Polr1a	UTSW	6	71,906,427 (GRCm39)	missense	possibly damaging	0.89
R6578:Polr1a	UTSW	6	71,953,025 (GRCm39)	missense	possibly damaging	0.93
R6660:Polr1a	UTSW	6	71,944,358 (GRCm39)	missense	probably damaging	0.98
R6892:Polr1a	UTSW	6	71,941,696 (GRCm39)	missense	possibly damaging	0.72
R7274:Polr1a	UTSW	6	71,897,500 (GRCm39)	nonsense	probably null
R7291:Polr1a	UTSW	6	71,918,440 (GRCm39)	missense	probably benign	0.02
R7311:Polr1a	UTSW	6	71,927,863 (GRCm39)	missense	possibly damaging	0.53
R7431:Polr1a	UTSW	6	71,903,643 (GRCm39)	missense	probably benign	0.14
R7479:Polr1a	UTSW	6	71,913,281 (GRCm39)	missense	probably damaging	1.00
R7607:Polr1a	UTSW	6	71,890,005 (GRCm39)	missense	probably benign
R7739:Polr1a	UTSW	6	71,931,819 (GRCm39)	missense	possibly damaging	0.94
R7746:Polr1a	UTSW	6	71,918,496 (GRCm39)	missense	probably damaging	1.00
R7764:Polr1a	UTSW	6	71,930,054 (GRCm39)	missense	probably damaging	1.00
R7835:Polr1a	UTSW	6	71,892,126 (GRCm39)	missense	probably benign	0.02
R8029:Polr1a	UTSW	6	71,889,940 (GRCm39)	nonsense	probably null
R8057:Polr1a	UTSW	6	71,908,644 (GRCm39)	missense	possibly damaging	0.95
R8144:Polr1a	UTSW	6	71,927,600 (GRCm39)	missense	probably benign
R8170:Polr1a	UTSW	6	71,897,733 (GRCm39)	missense	probably benign
R8320:Polr1a	UTSW	6	71,918,368 (GRCm39)	missense	probably damaging	0.99
R8328:Polr1a	UTSW	6	71,897,718 (GRCm39)	missense	probably benign
R8331:Polr1a	UTSW	6	71,953,163 (GRCm39)	missense	probably damaging	1.00
R8362:Polr1a	UTSW	6	71,941,651 (GRCm39)	missense	probably benign	0.00
R8511:Polr1a	UTSW	6	71,897,504 (GRCm39)	missense	probably benign	0.01
R8709:Polr1a	UTSW	6	71,951,832 (GRCm39)	missense	probably benign
R8745:Polr1a	UTSW	6	71,931,755 (GRCm39)	missense	probably damaging	1.00
R8784:Polr1a	UTSW	6	71,927,612 (GRCm39)	missense	probably benign
R9055:Polr1a	UTSW	6	71,892,053 (GRCm39)	missense	possibly damaging	0.46
R9088:Polr1a	UTSW	6	71,908,767 (GRCm39)	missense	probably benign	0.26
R9211:Polr1a	UTSW	6	71,943,521 (GRCm39)	missense	probably damaging	1.00
R9228:Polr1a	UTSW	6	71,931,755 (GRCm39)	missense	probably damaging	1.00
R9240:Polr1a	UTSW	6	71,940,661 (GRCm39)	nonsense	probably null
R9267:Polr1a	UTSW	6	71,942,542 (GRCm39)	missense	probably benign
R9302:Polr1a	UTSW	6	71,901,683 (GRCm39)	critical splice donor site	probably null
R9744:Polr1a	UTSW	6	71,906,372 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- GAAGGCTGCTTGAAGTAGACC -3'
(R):5'- CTGGTTCTCCAAGGAACATGAGG -3'

Sequencing Primer
(F):5'- TTGAAGTAGACCTGTAGGCCC -3'
(R):5'- AGGATTTGTCCCAGCACAG -3'

Posted On

2016-02-04