Mutagenetix > Incidental Mutation

Incidental Mutation 'R4825:Muc4'

371464

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

042441-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R4825 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32555089-32602761 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 32570565 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 542 (T542S)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: T542S

PolyPhen 2 Score 0.408 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: T542S

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 130 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	C	A	11: 109,682,498 (GRCm39)	L229F	probably benign	Het
A930002H24Rik	A	C	17: 64,170,603 (GRCm39)	S62A	unknown	Het
Abca12	T	A	1: 71,341,844 (GRCm39)	Q1039L	possibly damaging	Het
Adgrg5	T	A	8: 95,668,362 (GRCm39)	F476I	possibly damaging	Het
Alms1	A	G	6: 85,655,227 (GRCm39)	K2789E	probably damaging	Het
Arrdc2	G	A	8: 71,291,921 (GRCm39)		probably null	Het
Atp2b1	T	A	10: 98,845,426 (GRCm39)	I743K	probably damaging	Het
Atp6v1c2	C	T	12: 17,339,061 (GRCm39)	G230D	probably benign	Het
AU040320	G	A	4: 126,685,586 (GRCm39)	C54Y	probably damaging	Het
BC024139	C	T	15: 76,004,517 (GRCm39)	V680I	possibly damaging	Het
Bckdhb	A	G	9: 83,870,958 (GRCm39)	D156G	probably damaging	Het
Bltp3a	G	A	17: 28,096,368 (GRCm39)	A107T	probably damaging	Het
Canx	T	A	11: 50,199,636 (GRCm39)	D143V	probably benign	Het
Ccdc180	T	A	4: 45,912,794 (GRCm39)	V591E	possibly damaging	Het
Ccno	T	C	13: 113,124,633 (GRCm39)	S68P	probably benign	Het
Cdc45	C	T	16: 18,603,613 (GRCm39)	E527K	probably damaging	Het
Cep290	T	A	10: 100,324,210 (GRCm39)	D14E	probably damaging	Het
Cgnl1	A	G	9: 71,537,806 (GRCm39)	V1238A	probably benign	Het
Ciz1	C	T	2: 32,261,753 (GRCm39)	A455V	probably damaging	Het
Coro2b	T	A	9: 62,361,905 (GRCm39)	Y86F	probably benign	Het
Csf2ra	C	T	19: 61,214,990 (GRCm39)	R158Q	probably benign	Het
Cubn	A	T	2: 13,330,036 (GRCm39)	I2615N	probably damaging	Het
Dhx8	C	T	11: 101,628,996 (GRCm39)	R129*	probably null	Het
Disc1	T	A	8: 125,862,041 (GRCm39)	M471K	possibly damaging	Het
Dmxl2	G	T	9: 54,311,325 (GRCm39)	L1799I	probably benign	Het
Dnah2	A	G	11: 69,314,031 (GRCm39)	S4108P	probably damaging	Het
Ecpas	G	A	4: 58,850,911 (GRCm39)	L421F	probably damaging	Het
Ehmt2	C	G	17: 35,125,940 (GRCm39)	P211R	probably benign	Het
Eif4g3	A	G	4: 137,921,392 (GRCm39)	D1557G	probably benign	Het
Epha5	T	C	5: 84,381,699 (GRCm39)	D384G	probably damaging	Het
Etl4	A	G	2: 20,811,738 (GRCm39)	I1274V	probably damaging	Het
Fhip1a	G	A	3: 85,580,739 (GRCm39)	P489S	possibly damaging	Het
Fip1l1	G	A	5: 74,748,866 (GRCm39)		probably null	Het
Fubp1	T	C	3: 151,923,527 (GRCm39)		probably null	Het
Glul	T	C	1: 153,778,790 (GRCm39)	V33A	probably benign	Het
Gm4846	A	G	1: 166,319,237 (GRCm39)	F167S	probably damaging	Het
Heatr6	T	A	11: 83,649,148 (GRCm39)	L168M	probably damaging	Het
Hif1a	T	A	12: 73,979,175 (GRCm39)	I233N	probably damaging	Het
Hivep2	T	A	10: 14,007,063 (GRCm39)	H1220Q	possibly damaging	Het
Igkv8-21	T	C	6: 70,292,410 (GRCm39)	I9M	probably benign	Het
Izumo1	T	A	7: 45,274,411 (GRCm39)	C62*	probably null	Het
Jakmip3	G	A	7: 138,628,495 (GRCm39)	E424K	probably damaging	Het
Klhl2	A	G	8: 65,205,847 (GRCm39)	V358A	probably damaging	Het
Klk10	C	G	7: 43,433,022 (GRCm39)	D139E	probably damaging	Het
Klk14	T	C	7: 43,341,500 (GRCm39)	C51R	probably damaging	Het
Klk1b5	T	G	7: 43,494,814 (GRCm39)	I99S	probably damaging	Het
L3hypdh	T	C	12: 72,124,167 (GRCm39)	T258A	probably benign	Het
Lrp5	A	G	19: 3,664,292 (GRCm39)	Y812H	probably damaging	Het
Lrrc40	T	A	3: 157,766,967 (GRCm39)	L474*	probably null	Het
Mkks	A	T	2: 136,722,575 (GRCm39)	M194K	probably benign	Het
Mmp20	A	G	9: 7,654,121 (GRCm39)	D347G	probably damaging	Het
Mmp27	A	G	9: 7,581,195 (GRCm39)	E460G	probably damaging	Het
Mms22l	T	C	4: 24,536,226 (GRCm39)	F605S	probably damaging	Het
Mpzl3	A	G	9: 44,979,627 (GRCm39)	S193G	probably benign	Het
Muc5b	T	C	7: 141,422,202 (GRCm39)	L4446P	possibly damaging	Het
Mxi1	C	A	19: 53,358,769 (GRCm39)	S131*	probably null	Het
Nanog	G	T	6: 122,690,299 (GRCm39)	A210S	probably benign	Het
Nrcam	C	T	12: 44,622,769 (GRCm39)	Q988*	probably null	Het
Nsg1	C	A	5: 38,316,391 (GRCm39)		probably benign	Het
Ogfod3	G	A	11: 121,086,027 (GRCm39)	A189V	probably benign	Het
Or10g9b	A	T	9: 39,918,038 (GRCm39)	M69K	possibly damaging	Het
Or12e9	T	A	2: 87,202,432 (GRCm39)	C185*	probably null	Het
Or4a68	A	G	2: 89,270,209 (GRCm39)	V138A	probably benign	Het
Or4c122	T	C	2: 89,079,034 (GRCm39)		probably null	Het
Or4c35	G	A	2: 89,808,497 (GRCm39)	C125Y	probably damaging	Het
Or51ai2	T	C	7: 103,586,710 (GRCm39)	V41A	probably benign	Het
Or52b4i	T	A	7: 102,191,587 (GRCm39)	V148E	possibly damaging	Het
Or5b111	A	T	19: 13,291,684 (GRCm39)		probably null	Het
Or7e165	T	G	9: 19,694,872 (GRCm39)	S148A	possibly damaging	Het
Or8b53	A	T	9: 38,667,703 (GRCm39)	T240S	probably damaging	Het
Orc3	A	T	4: 34,571,774 (GRCm39)	M665K	possibly damaging	Het
Pabpc1	A	G	15: 36,597,255 (GRCm39)	S591P	probably damaging	Het
Pacc1	A	T	1: 191,073,040 (GRCm39)	I154F	probably damaging	Het
Parp6	T	A	9: 59,531,645 (GRCm39)		probably null	Het
Pcdh1	T	C	18: 38,322,912 (GRCm39)	M974V	possibly damaging	Het
Pcdhb22	T	A	18: 37,653,713 (GRCm39)	V727E	possibly damaging	Het
Pex5l	T	C	3: 33,047,134 (GRCm39)	E272G	probably damaging	Het
Pglyrp2	G	T	17: 32,637,235 (GRCm39)	N264K	probably benign	Het
Phxr4	T	A	9: 13,342,882 (GRCm39)		probably benign	Het
Piezo2	A	G	18: 63,278,025 (GRCm39)	F293S	probably damaging	Het
Pkhd1	T	C	1: 20,607,625 (GRCm39)	D1077G	probably damaging	Het
Plekhs1	A	G	19: 56,461,700 (GRCm39)		probably null	Het
Prex1	G	T	2: 166,427,777 (GRCm39)	C788*	probably null	Het
Prrt3	A	T	6: 113,475,099 (GRCm39)	M41K	probably benign	Het
Ptgir	T	C	7: 16,642,768 (GRCm39)	V326A	probably damaging	Het
Ptprg	T	A	14: 12,220,654 (GRCm38)	D455E	probably damaging	Het
Ptpru	T	A	4: 131,526,914 (GRCm39)	Q686L	probably benign	Het
Pxdc1	G	T	13: 34,814,343 (GRCm39)	T190K	probably benign	Het
Rap1b	A	T	10: 117,654,487 (GRCm39)	C118S	probably benign	Het
Rapgef2	T	C	3: 78,990,534 (GRCm39)	M915V	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rpe65	C	T	3: 159,330,318 (GRCm39)	A495V	probably benign	Het
Samd15	A	G	12: 87,247,608 (GRCm39)	T98A	possibly damaging	Het
Sdk1	T	G	5: 141,568,049 (GRCm39)	D82E	probably benign	Het
Slc26a7	T	C	4: 14,546,309 (GRCm39)	D340G	probably benign	Het
Slc6a15	T	A	10: 103,253,921 (GRCm39)	M619K	probably benign	Het
Slc7a4	C	A	16: 17,392,385 (GRCm39)	D350Y	probably damaging	Het
Slk	T	G	19: 47,608,395 (GRCm39)	N449K	probably benign	Het
Spta1	A	T	1: 174,071,608 (GRCm39)		probably null	Het
Sptbn5	A	T	2: 119,886,374 (GRCm39)		probably benign	Het
Srd5a2	A	T	17: 74,354,800 (GRCm39)	V8D	probably benign	Het
Srgap3	G	A	6: 112,704,271 (GRCm39)	A906V	probably benign	Het
Stab2	A	T	10: 86,783,011 (GRCm39)	M679K	probably benign	Het
Stk3	T	C	15: 35,000,054 (GRCm39)	I291V	probably benign	Het
Supt6	T	A	11: 78,098,960 (GRCm39)	Q1637L	possibly damaging	Het
Svil	T	C	18: 5,114,564 (GRCm39)	F2047S	probably damaging	Het
Swsap1	A	G	9: 21,867,284 (GRCm39)	E76G	probably benign	Het
Syndig1	G	T	2: 149,741,473 (GRCm39)	G20C	probably damaging	Het
Synj2bp	A	T	12: 81,548,926 (GRCm39)	N104K	probably damaging	Het
Synpo2	T	A	3: 122,908,068 (GRCm39)	D416V	probably damaging	Het
Tacc3	G	T	5: 33,829,357 (GRCm39)	C620F	probably damaging	Het
Tanc1	A	G	2: 59,529,766 (GRCm39)	E19G	probably damaging	Het
Tars3	C	T	7: 65,297,302 (GRCm39)	A139V	probably benign	Het
Tbc1d22a	T	A	15: 86,235,935 (GRCm39)	C365S	probably damaging	Het
Tdpoz2	T	C	3: 93,559,381 (GRCm39)	H197R	possibly damaging	Het
Tha1	A	T	11: 117,760,205 (GRCm39)	N300K	probably damaging	Het
Tm6sf1	T	A	7: 81,515,008 (GRCm39)	F70L	probably damaging	Het
Tmem132b	T	A	5: 125,860,497 (GRCm39)	S581T	probably benign	Het
Tnfsf13	G	T	11: 69,576,075 (GRCm39)	S4*	probably null	Het
Tonsl	G	A	15: 76,517,448 (GRCm39)	S757L	probably benign	Het
Trem2	G	T	17: 48,658,719 (GRCm39)	R161S	possibly damaging	Het
Trpm2	A	T	10: 77,777,007 (GRCm39)	V430E	probably damaging	Het
Ttc14	T	A	3: 33,855,518 (GRCm39)	D154E	possibly damaging	Het
Ugt2b37	T	C	5: 87,398,498 (GRCm39)	M313V	possibly damaging	Het
Vmn2r24	A	G	6: 123,792,739 (GRCm39)	I689V	probably benign	Het
Wdr76	A	G	2: 121,372,975 (GRCm39)	T601A	probably benign	Het
Xirp1	T	C	9: 119,846,069 (GRCm39)	D938G	possibly damaging	Het
Zfp608	A	T	18: 55,031,041 (GRCm39)	D966E	probably benign	Het
Zfp957	A	G	14: 79,451,796 (GRCm39)	M1T	probably null	Het
Zkscan16	A	T	4: 58,957,809 (GRCm39)	H697L	possibly damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,599,186 (GRCm39)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,574,613 (GRCm39)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,569,728 (GRCm39)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,575,465 (GRCm39)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,140 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,139 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,114 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,576,179 (GRCm39)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,575,246 (GRCm39)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,575,362 (GRCm39)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,584,340 (GRCm39)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,569,473 (GRCm39)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,577,001 (GRCm39)	missense	unknown
IGL01625:Muc4	APN	16	32,575,918 (GRCm39)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,555,220 (GRCm39)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,581,722 (GRCm39)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,574,624 (GRCm39)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,570,244 (GRCm39)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,575,383 (GRCm39)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,598,023 (GRCm39)	splice site	probably benign
IGL02210:Muc4	APN	16	32,574,054 (GRCm39)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,569,668 (GRCm39)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,570,894 (GRCm39)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,576,503 (GRCm39)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,596,193 (GRCm39)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,575,656 (GRCm39)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,570,466 (GRCm39)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,569,212 (GRCm39)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,570,866 (GRCm39)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,570,766 (GRCm39)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,570,257 (GRCm39)	nonsense	probably null
IGL03335:Muc4	APN	16	32,574,449 (GRCm39)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,575,436 (GRCm39)	missense	probably benign	0.01
multisplendored	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,590,768 (GRCm39)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,580,625 (GRCm39)	splice site	probably benign
BB004:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
BB014:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
IGL02835:Muc4	UTSW	16	32,584,319 (GRCm39)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,580,622 (GRCm39)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,575,542 (GRCm39)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,576,391 (GRCm39)	missense	unknown
R0119:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,591,978 (GRCm39)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,586,120 (GRCm39)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,574,333 (GRCm39)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,582,910 (GRCm39)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,569,887 (GRCm39)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,577,120 (GRCm39)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,570,925 (GRCm39)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,570,488 (GRCm39)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,590,201 (GRCm39)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,590,221 (GRCm39)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,599,329 (GRCm39)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,576,570 (GRCm39)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,569,343 (GRCm39)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,574,448 (GRCm39)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,574,033 (GRCm39)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,569,167 (GRCm39)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,575,875 (GRCm39)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,577,276 (GRCm39)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,576,307 (GRCm39)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,576,625 (GRCm39)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,576,715 (GRCm39)	missense	unknown
R1928:Muc4	UTSW	16	32,569,350 (GRCm39)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,569,460 (GRCm39)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,570,121 (GRCm39)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,574,054 (GRCm39)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,576,783 (GRCm39)	missense	unknown
R2121:Muc4	UTSW	16	32,580,612 (GRCm39)	missense	unknown
R2139:Muc4	UTSW	16	32,581,599 (GRCm39)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,569,294 (GRCm39)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,587,316 (GRCm39)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,576,265 (GRCm39)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,577,846 (GRCm39)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,575,647 (GRCm39)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,576,200 (GRCm39)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,590,666 (GRCm39)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,577,075 (GRCm39)	missense	unknown
R4523:Muc4	UTSW	16	32,555,154 (GRCm39)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,576,217 (GRCm39)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,577,432 (GRCm39)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,589,651 (GRCm39)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,576,220 (GRCm39)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,570,032 (GRCm39)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,596,277 (GRCm39)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,574,916 (GRCm39)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,576,472 (GRCm39)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,575,437 (GRCm39)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,575,332 (GRCm39)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,576,670 (GRCm39)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,577,432 (GRCm39)	nonsense	probably null
R5161:Muc4	UTSW	16	32,582,895 (GRCm39)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,570,556 (GRCm39)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,570,484 (GRCm39)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,581,635 (GRCm39)	missense	unknown
R5567:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,569,600 (GRCm39)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,597,181 (GRCm39)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,555,059 (GRCm39)	nonsense	probably null
R5708:Muc4	UTSW	16	32,575,515 (GRCm39)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,570,734 (GRCm39)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,595,213 (GRCm39)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,570,113 (GRCm39)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,570,523 (GRCm39)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,574,480 (GRCm39)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,576,652 (GRCm39)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,579,682 (GRCm39)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,596,157 (GRCm39)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,577,169 (GRCm39)	missense	unknown
R6144:Muc4	UTSW	16	32,587,298 (GRCm39)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,554,958 (GRCm39)	start gained	probably benign
R6268:Muc4	UTSW	16	32,589,141 (GRCm39)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,570,853 (GRCm39)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,575,237 (GRCm39)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,575,476 (GRCm39)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,587,725 (GRCm39)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,555,061 (GRCm39)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,599,320 (GRCm39)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,570,607 (GRCm39)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,579,694 (GRCm39)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,602,378 (GRCm39)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,587,312 (GRCm39)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,596,177 (GRCm39)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,582,892 (GRCm39)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,570,817 (GRCm39)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,570,628 (GRCm39)	nonsense	probably null
R7033:Muc4	UTSW	16	32,576,698 (GRCm39)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,577,465 (GRCm39)	missense
R7123:Muc4	UTSW	16	32,569,509 (GRCm39)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,582,862 (GRCm39)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,576,835 (GRCm39)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,575,710 (GRCm39)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,575,740 (GRCm39)	missense	unknown
R7381:Muc4	UTSW	16	32,601,285 (GRCm39)	missense
R7411:Muc4	UTSW	16	32,570,140 (GRCm39)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,587,324 (GRCm39)	missense
R7539:Muc4	UTSW	16	32,576,770 (GRCm39)	missense
R7544:Muc4	UTSW	16	32,555,016 (GRCm39)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,586,076 (GRCm39)	missense
R7596:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,574,161 (GRCm39)	nonsense	probably null
R7639:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,580,479 (GRCm39)	missense
R7651:Muc4	UTSW	16	32,576,949 (GRCm39)	missense
R7688:Muc4	UTSW	16	32,570,278 (GRCm39)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,574,439 (GRCm39)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,589,108 (GRCm39)	missense
R7763:Muc4	UTSW	16	32,574,739 (GRCm39)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,576,568 (GRCm39)	missense	unknown
R7787:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,570,139 (GRCm39)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
R7930:Muc4	UTSW	16	32,575,369 (GRCm39)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,577,123 (GRCm39)	missense
R8096:Muc4	UTSW	16	32,575,764 (GRCm39)	missense	unknown
R8115:Muc4	UTSW	16	32,575,678 (GRCm39)	missense	unknown
R8162:Muc4	UTSW	16	32,569,197 (GRCm39)	missense	unknown
R8220:Muc4	UTSW	16	32,575,701 (GRCm39)	missense	unknown
R8260:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,575,434 (GRCm39)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,576,271 (GRCm39)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,574,201 (GRCm39)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,574,799 (GRCm39)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,574,421 (GRCm39)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,575,629 (GRCm39)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,602,356 (GRCm39)	missense
R8845:Muc4	UTSW	16	32,576,889 (GRCm39)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,569,461 (GRCm39)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,570,280 (GRCm39)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,575,285 (GRCm39)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,574,009 (GRCm39)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,582,910 (GRCm39)	missense
R9034:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,577,842 (GRCm39)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,589,102 (GRCm39)	missense
R9336:Muc4	UTSW	16	32,569,756 (GRCm39)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,569,971 (GRCm39)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,576,992 (GRCm39)	missense
R9429:Muc4	UTSW	16	32,576,098 (GRCm39)	missense	unknown
R9570:Muc4	UTSW	16	32,569,695 (GRCm39)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,577,234 (GRCm39)	missense
R9696:Muc4	UTSW	16	32,574,712 (GRCm39)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,590,644 (GRCm39)	missense
R9743:Muc4	UTSW	16	32,601,194 (GRCm39)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,570,676 (GRCm39)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,570,643 (GRCm39)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,575,855 (GRCm39)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,579,693 (GRCm39)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,576,450 (GRCm39)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,587,767 (GRCm39)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,595,236 (GRCm39)	missense
Z1177:Muc4	UTSW	16	32,587,768 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- AGAACAAACTCTCCTGGCG -3'
(R):5'- TGAGGTGGTTGTCTCACCAG -3'

Sequencing Primer
(F):5'- TCTCCTGGCGAGAAAAGGACATC -3'
(R):5'- CACCAGGAGAGATTGTGTTTTG -3'

Posted On

2016-03-01