Mutagenetix > Incidental Mutation

Incidental Mutation 'R4842:Vmn1r173'

371915

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r173

Ensembl Gene

ENSMUSG00000115021

Gene Name

vomeronasal 1 receptor 173

Synonyms

Gm5892

MMRRC Submission

042455-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.670) question?

Stock #

R4842 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23401767-23402708 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23402361 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 199 (I199F)

Ref Sequence

ENSEMBL: ENSMUSP00000153884 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000174055] [ENSMUST00000226233] [ENSMUST00000227987]

AlphaFold

E9Q8V7

Predicted Effect

probably damaging
Transcript: ENSMUST00000174055
AA Change: I199F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134645
Gene: ENSMUSG00000115021
AA Change: I199F

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
Pfam:V1R	43	301	5.3e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000226233
AA Change: I199F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000227987
AA Change: I199F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.7%
20x: 93.6%

Validation Efficiency

95% (107/113)

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600012H06Rik	A	T	17: 15,164,001 (GRCm39)	I43L	possibly damaging	Het
4930449A18Rik	T	A	3: 59,749,153 (GRCm39)		noncoding transcript	Het
Abcc8	T	C	7: 45,800,252 (GRCm39)	K510R	probably damaging	Het
Adamtsl3	C	T	7: 82,178,069 (GRCm39)	R511C	probably damaging	Het
Arhgap11a	T	C	2: 113,670,107 (GRCm39)	S339G	probably damaging	Het
C2cd3	A	G	7: 100,065,397 (GRCm39)	T728A	probably benign	Het
Cand1	C	A	10: 119,049,451 (GRCm39)		probably null	Het
Catspere1	A	T	1: 177,699,624 (GRCm39)		noncoding transcript	Het
Ccdc121rt2	A	T	5: 112,598,106 (GRCm39)	K218*	probably null	Het
Cd209c	G	T	8: 3,995,905 (GRCm39)	R2S	probably benign	Het
Cdc16	A	G	8: 13,831,644 (GRCm39)		probably benign	Het
Ces1g	C	T	8: 94,060,323 (GRCm39)	E99K	probably benign	Het
Chrna7	A	C	7: 62,862,196 (GRCm39)	L10R	probably benign	Het
Clnk	C	T	5: 38,870,412 (GRCm39)		probably null	Het
Cntrob	C	G	11: 69,206,220 (GRCm39)	L315F	possibly damaging	Het
Ctdp1	A	G	18: 80,451,941 (GRCm39)	S145P	unknown	Het
Dennd2a	T	C	6: 39,474,044 (GRCm39)	D430G	probably damaging	Het
Dnajc16	A	G	4: 141,501,936 (GRCm39)	F298S	probably damaging	Het
Dock5	T	C	14: 68,055,012 (GRCm39)	D618G	probably damaging	Het
Drc3	G	A	11: 60,261,361 (GRCm39)	A171T	probably benign	Het
Ecel1	A	T	1: 87,081,023 (GRCm39)	N322K	probably damaging	Het
Eftud2	A	G	11: 102,745,640 (GRCm39)	F362L	probably damaging	Het
Egfr	C	T	11: 16,861,607 (GRCm39)	H1129Y	probably benign	Het
Eif2b1	A	G	5: 124,714,971 (GRCm39)	S102P	probably damaging	Het
Erich3	T	A	3: 154,410,480 (GRCm39)	F112I	possibly damaging	Het
Fam107b	T	C	2: 3,779,580 (GRCm39)	L261S	probably damaging	Het
Fat3	A	C	9: 15,908,883 (GRCm39)	V2373G	probably damaging	Het
Fcgbpl1	T	A	7: 27,850,147 (GRCm39)	C1198S	probably damaging	Het
Gadd45a	A	T	6: 67,013,873 (GRCm39)	L58Q	probably damaging	Het
Gask1b	G	A	3: 79,843,912 (GRCm39)	R377H	probably damaging	Het
Gipr	C	T	7: 18,896,601 (GRCm39)	R165H	probably damaging	Het
Gje1	C	T	10: 14,593,082 (GRCm39)	G45R	probably null	Het
Gldc	T	A	19: 30,111,132 (GRCm39)	N548I	possibly damaging	Het
Gm13035	A	G	4: 146,009,993 (GRCm39)		noncoding transcript	Het
Gm27013	T	A	6: 130,497,700 (GRCm39)		noncoding transcript	Het
Gm5436	A	T	12: 84,305,584 (GRCm39)		noncoding transcript	Het
Grik3	C	A	4: 125,584,969 (GRCm39)	N612K	probably damaging	Het
H2bc13	C	T	13: 21,900,234 (GRCm39)		probably benign	Het
Hfm1	C	A	5: 107,040,617 (GRCm39)	W716L	probably damaging	Het
Il5ra	T	C	6: 106,715,336 (GRCm39)	Y166C	probably damaging	Het
Iqcb1	A	G	16: 36,655,952 (GRCm39)	E113G	probably benign	Het
Kcnk10	A	T	12: 98,401,175 (GRCm39)	M486K	probably benign	Het
Kcnv2	A	G	19: 27,301,190 (GRCm39)	D347G	probably damaging	Het
Kif21b	C	A	1: 136,072,958 (GRCm39)	H119N	probably damaging	Het
Lamb1	C	T	12: 31,337,432 (GRCm39)	H388Y	probably damaging	Het
Leng9	T	C	7: 4,152,385 (GRCm39)	D97G	probably damaging	Het
Lmbr1	G	A	5: 29,492,424 (GRCm39)	T55I	probably damaging	Het
Lrp1	G	T	10: 127,419,805 (GRCm39)	R935S	probably damaging	Het
Lrp2	A	T	2: 69,299,755 (GRCm39)	V3099E	probably benign	Het
Lrrcc1	C	A	3: 14,627,571 (GRCm39)	D503E	probably benign	Het
Map1a	T	A	2: 121,132,567 (GRCm39)	S890T	probably damaging	Het
Msh2	C	A	17: 88,030,841 (GRCm39)	A906E	probably benign	Het
Mybph	A	T	1: 134,126,233 (GRCm39)	E349V	probably damaging	Het
Myh7b	C	A	2: 155,475,909 (GRCm39)	L1935M	probably benign	Het
Myh9	T	C	15: 77,653,453 (GRCm39)	E1348G	probably damaging	Het
Myzap	A	G	9: 71,456,037 (GRCm39)	S328P	probably damaging	Het
Nbeal1	T	C	1: 60,292,534 (GRCm39)	L1062P	probably damaging	Het
Nepro	G	A	16: 44,555,160 (GRCm39)	S412N	probably null	Het
Nr1h3	A	G	2: 91,020,563 (GRCm39)	F257L	probably benign	Het
Nudt5	T	C	2: 5,869,239 (GRCm39)	V155A	probably benign	Het
Ofcc1	G	A	13: 40,168,864 (GRCm39)	T841I	probably damaging	Het
Or13a25	A	T	7: 140,247,502 (GRCm39)	I94F	probably damaging	Het
Or52d3	A	T	7: 104,229,422 (GRCm39)	I190L	probably benign	Het
Or6ae1	A	G	7: 139,742,602 (GRCm39)	L87P	possibly damaging	Het
Pde1a	T	A	2: 79,959,181 (GRCm39)		probably benign	Het
Pde4dip	T	A	3: 97,700,844 (GRCm39)	H220L	probably damaging	Het
Pde9a	T	A	17: 31,662,135 (GRCm39)		probably null	Het
Pnkp	C	A	7: 44,511,070 (GRCm39)		probably null	Het
Pnpla7	A	G	2: 24,870,064 (GRCm39)	T15A	probably benign	Het
Polq	G	T	16: 36,869,145 (GRCm39)		probably null	Het
Ppfia2	C	T	10: 106,690,818 (GRCm39)	T553I	probably benign	Het
Ptk6	T	G	2: 180,838,784 (GRCm39)	N323T	possibly damaging	Het
Rhox3c	G	A	X: 36,652,077 (GRCm39)	A60T	probably damaging	Het
Rnf4	T	A	5: 34,506,053 (GRCm39)	V61E	probably damaging	Het
Rnf5	A	G	17: 34,820,977 (GRCm39)		probably benign	Het
Sardh	A	G	2: 27,081,967 (GRCm39)	V853A	probably benign	Het
Scfd1	T	C	12: 51,436,109 (GRCm39)	V86A	probably damaging	Het
Scube3	G	A	17: 28,383,097 (GRCm39)	C425Y	probably damaging	Het
Sema5a	C	T	15: 32,609,563 (GRCm39)	H490Y	probably benign	Het
Sfta2	T	C	17: 35,960,773 (GRCm39)		probably benign	Het
Sh3d19	T	C	3: 86,031,049 (GRCm39)	Y738H	probably damaging	Het
Shc2	T	C	10: 79,458,295 (GRCm39)	R463G	probably damaging	Het
Socs7	T	A	11: 97,267,829 (GRCm39)	I320N	possibly damaging	Het
Speer2	A	T	16: 69,654,988 (GRCm39)	M159K	probably benign	Het
Sppl2c	A	C	11: 104,078,478 (GRCm39)	H426P	probably benign	Het
Stag3	T	G	5: 138,307,627 (GRCm39)		probably null	Het
Stxbp5	C	A	10: 9,638,635 (GRCm39)	V1055L	probably benign	Het
Synpo	A	T	18: 60,736,684 (GRCm39)	S421T	probably damaging	Het
Taf6l	A	G	19: 8,759,770 (GRCm39)	V135A	possibly damaging	Het
Tas2r116	G	A	6: 132,832,660 (GRCm39)	S87N	probably benign	Het
Tomm6	T	C	17: 47,998,994 (GRCm39)		probably benign	Het
Trabd	T	C	15: 88,966,915 (GRCm39)	M113T	probably benign	Het
Trim58	G	A	11: 58,542,150 (GRCm39)	G370E	probably damaging	Het
Ttc41	C	T	10: 86,566,989 (GRCm39)	R552C	probably benign	Het
Vit	T	C	17: 78,909,308 (GRCm39)	S252P	probably benign	Het
Vma21-ps	T	A	4: 52,496,943 (GRCm39)	D101V	probably damaging	Het
Vmn2r114	T	A	17: 23,529,336 (GRCm39)	R255S	probably benign	Het
Vmn2r17	A	G	5: 109,582,246 (GRCm39)	N545S	probably damaging	Het
Zfp865	A	G	7: 5,034,640 (GRCm39)	Y875C	probably damaging	Het

Other mutations in Vmn1r173

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01490:Vmn1r173	APN	7	23,402,132 (GRCm39)	missense	probably benign	0.01
IGL01637:Vmn1r173	APN	7	23,402,373 (GRCm39)	missense	probably damaging	0.96
IGL01878:Vmn1r173	APN	7	23,401,877 (GRCm39)	missense	probably damaging	1.00
IGL02036:Vmn1r173	APN	7	23,402,321 (GRCm39)	missense	probably benign	0.04
IGL02039:Vmn1r173	APN	7	23,402,321 (GRCm39)	missense	probably benign	0.04
IGL02171:Vmn1r173	APN	7	23,402,321 (GRCm39)	missense	probably benign	0.04
IGL02209:Vmn1r173	APN	7	23,402,586 (GRCm39)	missense	probably benign	0.25
PIT4515001:Vmn1r173	UTSW	7	23,401,911 (GRCm39)	nonsense	probably null
R0157:Vmn1r173	UTSW	7	23,401,822 (GRCm39)	missense	probably damaging	0.99
R0226:Vmn1r173	UTSW	7	23,402,508 (GRCm39)	missense	possibly damaging	0.65
R0482:Vmn1r173	UTSW	7	23,402,216 (GRCm39)	missense	probably damaging	0.99
R0792:Vmn1r173	UTSW	7	23,402,160 (GRCm39)	missense	probably benign	0.01
R1242:Vmn1r173	UTSW	7	23,402,650 (GRCm39)	missense	probably damaging	1.00
R1390:Vmn1r173	UTSW	7	23,402,323 (GRCm39)	missense	possibly damaging	0.82
R1641:Vmn1r173	UTSW	7	23,402,533 (GRCm39)	missense	probably benign	0.06
R1867:Vmn1r173	UTSW	7	23,402,660 (GRCm39)	missense	unknown
R2325:Vmn1r173	UTSW	7	23,402,537 (GRCm39)	nonsense	probably null
R3863:Vmn1r173	UTSW	7	23,401,977 (GRCm39)	missense	probably damaging	1.00
R4407:Vmn1r173	UTSW	7	23,402,441 (GRCm39)	missense	probably damaging	1.00
R4717:Vmn1r173	UTSW	7	23,402,637 (GRCm39)	missense	probably damaging	1.00
R4841:Vmn1r173	UTSW	7	23,402,361 (GRCm39)	missense	probably damaging	1.00
R5966:Vmn1r173	UTSW	7	23,402,112 (GRCm39)	missense	probably benign	0.00
R6022:Vmn1r173	UTSW	7	23,402,260 (GRCm39)	missense	probably benign	0.07
R6114:Vmn1r173	UTSW	7	23,402,254 (GRCm39)	missense	possibly damaging	0.53
R6657:Vmn1r173	UTSW	7	23,402,320 (GRCm39)	missense	probably damaging	0.98
R7165:Vmn1r173	UTSW	7	23,402,076 (GRCm39)	missense	probably benign	0.00
R7195:Vmn1r173	UTSW	7	23,401,884 (GRCm39)	missense	probably damaging	0.99
R7201:Vmn1r173	UTSW	7	23,401,583 (GRCm39)	start gained	probably benign
R7533:Vmn1r173	UTSW	7	23,402,071 (GRCm39)	missense	probably benign	0.05
R7951:Vmn1r173	UTSW	7	23,402,680 (GRCm39)	missense	unknown
R8351:Vmn1r173	UTSW	7	23,401,957 (GRCm39)	nonsense	probably null
R8374:Vmn1r173	UTSW	7	23,401,920 (GRCm39)	missense	probably damaging	0.98
R8427:Vmn1r173	UTSW	7	23,401,959 (GRCm39)	missense	probably damaging	0.99
R8451:Vmn1r173	UTSW	7	23,401,957 (GRCm39)	nonsense	probably null
R8923:Vmn1r173	UTSW	7	23,401,768 (GRCm39)	start codon destroyed	probably null	1.00
R9126:Vmn1r173	UTSW	7	23,402,008 (GRCm39)	missense	probably benign	0.23
R9506:Vmn1r173	UTSW	7	23,401,963 (GRCm39)	missense	probably damaging	0.99
R9557:Vmn1r173	UTSW	7	23,402,209 (GRCm39)	missense	probably damaging	1.00
X0022:Vmn1r173	UTSW	7	23,402,012 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- GGAAATACCTCCACATTTGTGC -3'
(R):5'- GGGAAACTTGCAGCCAAAATTTC -3'

Sequencing Primer
(F):5'- AATACCTCCACATTTGTGCACTATTC -3'
(R):5'- GCAGCCAAAATTTCATTGACATGC -3'

Posted On

2016-03-01