Mutagenetix > Incidental Mutation

Incidental Mutation 'R4439:Wdr86'

374413

Institutional Source

Beutler Lab

Gene Symbol

Wdr86

Ensembl Gene

ENSMUSG00000055235

Gene Name

WD repeat domain 86

Synonyms

2810046M22Rik

MMRRC Submission

041704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R4439 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

24916736-24935725 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 24935235 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 36 (D36G)

Ref Sequence

ENSEMBL: ENSMUSP00000064785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068693]

AlphaFold

D3Z757

Predicted Effect

probably damaging
Transcript: ENSMUST00000068693
AA Change: D36G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000064785
Gene: ENSMUSG00000055235
AA Change: D36G

Domain	Start	End	E-Value	Type
WD40	4	43	1.62e-8	SMART
WD40	46	83	3.17e-2	SMART
WD40	86	123	1.9e-5	SMART
WD40	126	179	3e-3	SMART
WD40	182	221	2.78e-7	SMART
WD40	224	261	9.9e-4	SMART
WD40	264	301	1.29e-2	SMART
WD40	304	341	6.28e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142607

Meta Mutation Damage Score

0.8189

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.8%

Validation Efficiency

94% (50/53)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	C	T	5: 114,384,557 (GRCm39)	T2237I	possibly damaging	Het
Adgre1	T	A	17: 57,754,954 (GRCm39)	L684Q	probably damaging	Het
Cfap157	T	A	2: 32,667,877 (GRCm39)	Y488F	probably benign	Het
D630045J12Rik	A	G	6: 38,171,696 (GRCm39)	I824T	probably benign	Het
Eno2	T	C	6: 124,739,922 (GRCm39)		probably benign	Het
Fam78b	C	A	1: 166,906,491 (GRCm39)	Q217K	probably damaging	Het
Fpgs	C	T	2: 32,577,513 (GRCm39)	C219Y	probably damaging	Het
Garem2	G	T	5: 30,318,344 (GRCm39)	V106L	possibly damaging	Het
Grk3	A	T	5: 113,094,543 (GRCm39)		probably null	Het
H3c2	A	T	13: 23,936,708 (GRCm39)		probably null	Het
Hint2	T	A	4: 43,654,919 (GRCm39)	Y70F	probably damaging	Het
Ipp	A	G	4: 116,372,274 (GRCm39)	N101S	probably benign	Het
Kcnj1	A	T	9: 32,305,414 (GRCm39)		probably benign	Het
Kcnk4	T	C	19: 6,910,129 (GRCm39)	D44G	probably benign	Het
Kif5c	T	C	2: 49,578,737 (GRCm39)	S122P	possibly damaging	Het
Mideas	C	T	12: 84,203,245 (GRCm39)	G886S	probably benign	Het
Nipbl	T	A	15: 8,368,208 (GRCm39)	K1171N	probably damaging	Het
Or4k39	T	A	2: 111,239,653 (GRCm39)		noncoding transcript	Het
Pcf11	A	C	7: 92,307,225 (GRCm39)	L981R	probably damaging	Het
Pias2	T	A	18: 77,185,399 (GRCm39)	L153H	probably damaging	Het
Pjvk	T	C	2: 76,481,750 (GRCm39)	S68P	probably damaging	Het
Pkd1	T	C	17: 24,804,666 (GRCm39)	V3130A	probably damaging	Het
Plpp4	C	A	7: 128,858,813 (GRCm39)		probably benign	Het
Plxnd1	T	A	6: 115,970,937 (GRCm39)	H277L	probably damaging	Het
Pramel26	A	T	4: 143,538,143 (GRCm39)	V276E	possibly damaging	Het
Rasgrf2	T	C	13: 92,131,797 (GRCm39)	D620G	possibly damaging	Het
Rd3l	C	A	12: 111,946,092 (GRCm39)	S63I	possibly damaging	Het
Scfd2	G	C	5: 74,558,368 (GRCm39)	A503G	possibly damaging	Het
Slco4a1	T	C	2: 180,114,455 (GRCm39)	V549A	probably benign	Het
Tenm4	A	G	7: 96,545,022 (GRCm39)	N2375S	probably benign	Het
Tespa1	C	T	10: 130,197,826 (GRCm39)	R283C	probably damaging	Het
Tle2	A	G	10: 81,417,516 (GRCm39)	E227G	possibly damaging	Het
Tnn	T	G	1: 159,943,650 (GRCm39)	E1054D	probably benign	Het
Tnrc6a	A	T	7: 122,751,405 (GRCm39)	K54*	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tpr	T	A	1: 150,279,712 (GRCm39)	D206E	probably benign	Het
Ugt2b34	A	T	5: 87,040,726 (GRCm39)	F399I	probably damaging	Het
Usp3	A	T	9: 66,425,776 (GRCm39)	D456E	probably benign	Het
Vmn1r60	T	A	7: 5,547,488 (GRCm39)	H204L	probably damaging	Het
Vmn2r97	G	T	17: 19,150,616 (GRCm39)	A488S	probably benign	Het
Vrk3	C	T	7: 44,424,866 (GRCm39)	T427M	probably benign	Het
Zc3h4	C	T	7: 16,163,036 (GRCm39)	P479S	unknown	Het
Zfhx4	A	T	3: 5,279,875 (GRCm39)		probably benign	Het
Zfp607b	T	A	7: 27,402,149 (GRCm39)	C202S	probably damaging	Het
Zfp882	T	A	8: 72,667,453 (GRCm39)	F93L	probably damaging	Het

Other mutations in Wdr86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02419:Wdr86	APN	5	24,927,702 (GRCm39)	missense	probably damaging	1.00
R0412:Wdr86	UTSW	5	24,923,232 (GRCm39)	missense	probably benign	0.01
R0899:Wdr86	UTSW	5	24,923,005 (GRCm39)	missense	probably benign	0.15
R2520:Wdr86	UTSW	5	24,917,573 (GRCm39)	makesense	probably null
R3522:Wdr86	UTSW	5	24,923,305 (GRCm39)	missense	probably benign	0.00
R3686:Wdr86	UTSW	5	24,923,339 (GRCm39)	missense	probably damaging	1.00
R4657:Wdr86	UTSW	5	24,923,229 (GRCm39)	missense	probably benign	0.00
R4989:Wdr86	UTSW	5	24,917,843 (GRCm39)	splice site	probably null
R5649:Wdr86	UTSW	5	24,923,085 (GRCm39)	missense	probably benign
R5940:Wdr86	UTSW	5	24,927,660 (GRCm39)	missense	probably damaging	1.00
R6254:Wdr86	UTSW	5	24,923,281 (GRCm39)	missense	probably benign	0.04
R8012:Wdr86	UTSW	5	24,935,177 (GRCm39)	critical splice donor site	probably null
R8395:Wdr86	UTSW	5	24,935,187 (GRCm39)	missense	probably damaging	1.00
R9497:Wdr86	UTSW	5	24,920,538 (GRCm39)	missense	probably benign	0.01
Z1177:Wdr86	UTSW	5	24,917,746 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAAAGGATCTTTCATTGGGGTGC -3'
(R):5'- AGAGTCATAATCCTTGTTCCCCG -3'

Sequencing Primer
(F):5'- TTTGGTGCGACCCACACAC -3'
(R):5'- TCGAGCCTTTGCAGGAAC -3'

Posted On

2016-03-10