Mutagenetix > Incidental Mutation

Incidental Mutation 'R4863:Lama3'

374707

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

042473-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4863 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12466876-12716070 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 12672850 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 2481 (A2481V)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070] [ENSMUST00000188815]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000092070
AA Change: A2481V

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: A2481V

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188815
AA Change: A875V

PolyPhen 2 Score 0.035 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000140104
Gene: ENSMUSG00000024421
AA Change: A875V

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_Lam	78	122	2.66e-10	SMART
EGF_Lam	125	175	7.81e-8	SMART
Pfam:Laminin_I	230	496	1e-90	PFAM
low complexity region	579	594	N/A	INTRINSIC
coiled coil region	605	632	N/A	INTRINSIC
LamG	800	960	1.67e-2	SMART
LamG	1008	1136	1.72e-17	SMART
LamG	1179	1294	3.96e-17	SMART
LamG	1399	1527	1.12e-34	SMART
LamG	1569	1702	3.41e-30	SMART

Meta Mutation Damage Score

0.2254

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

99% (105/106)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 97 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930578I06Rik	C	T	14: 64,210,658 (GRCm39)	R190H	probably benign	Het
Abcc12	T	A	8: 87,265,005 (GRCm39)	I647F	probably damaging	Het
Acnat2	C	A	4: 49,380,172 (GRCm39)	W384L	probably damaging	Het
Acvr1	A	G	2: 58,367,723 (GRCm39)	L146P	possibly damaging	Het
Ankrd16	T	A	2: 11,789,127 (GRCm39)	M238K	probably benign	Het
Clmn	A	T	12: 104,763,353 (GRCm39)	I91N	probably damaging	Het
Cog8	A	T	8: 107,776,806 (GRCm39)	L523H	probably damaging	Het
Cpxm2	T	A	7: 131,661,476 (GRCm39)	K437M	probably benign	Het
Dhx57	A	T	17: 80,560,540 (GRCm39)	V999E	probably damaging	Het
Dnpep	T	C	1: 75,285,874 (GRCm39)		probably benign	Het
Dok3	G	A	13: 55,671,270 (GRCm39)	R434W	probably damaging	Het
Dpysl5	C	T	5: 30,941,687 (GRCm39)	H275Y	probably benign	Het
Ednra	A	T	8: 78,394,012 (GRCm39)	N361K	probably damaging	Het
Ei24	A	T	9: 36,695,861 (GRCm39)	S210R	probably damaging	Het
Erich3	T	A	3: 154,470,441 (GRCm39)	V158E	unknown	Het
Fam193a	T	A	5: 34,623,549 (GRCm39)	V1379E	possibly damaging	Het
Fasn	A	G	11: 120,699,654 (GRCm39)	V2304A	probably damaging	Het
Fcgbp	A	C	7: 27,785,769 (GRCm39)	D402A	probably benign	Het
Fkbp14	T	C	6: 54,562,930 (GRCm39)		probably benign	Het
Fnip1	G	A	11: 54,406,382 (GRCm39)	V1160I	possibly damaging	Het
Fsd2	T	C	7: 81,202,712 (GRCm39)	K289R	probably null	Het
Fuca2	A	T	10: 13,381,651 (GRCm39)	D188V	probably damaging	Het
Gfpt2	T	C	11: 49,701,797 (GRCm39)	V116A	probably benign	Het
Gm10770	T	A	2: 150,020,816 (GRCm39)	K234*	probably null	Het
Gm9887	A	G	12: 69,418,763 (GRCm39)		probably benign	Het
Gtf3c2	A	G	5: 31,316,577 (GRCm39)		probably benign	Het
H2-M10.3	T	C	17: 36,677,528 (GRCm39)	D250G	probably damaging	Het
Hapln4	G	A	8: 70,537,142 (GRCm39)	V26M	possibly damaging	Het
Hook3	G	A	8: 26,528,057 (GRCm39)	A611V	probably damaging	Het
Hr	T	C	14: 70,809,412 (GRCm39)	L1141P	probably damaging	Het
Ifngr1	T	C	10: 19,485,164 (GRCm39)	S388P	probably damaging	Het
Itga3	T	A	11: 94,952,793 (GRCm39)	Q326L	probably damaging	Het
Itgb3	T	A	11: 104,556,346 (GRCm39)	I729N	probably damaging	Het
Kcnab2	A	G	4: 152,486,403 (GRCm39)	S132P	probably damaging	Het
Lce1e	A	T	3: 92,615,178 (GRCm39)	C56*	probably null	Het
Lmcd1	T	C	6: 112,264,832 (GRCm39)		probably benign	Het
Lrrc14	T	A	15: 76,597,562 (GRCm39)		probably null	Het
Lypd8l	A	T	11: 58,503,338 (GRCm39)		probably null	Het
Map4k5	G	A	12: 69,865,212 (GRCm39)	P591L	probably benign	Het
Mapk13	C	A	17: 28,995,284 (GRCm39)	D168E	probably damaging	Het
Marf1	A	C	16: 13,950,529 (GRCm39)	H952Q	possibly damaging	Het
Megf6	T	C	4: 154,338,738 (GRCm39)		probably null	Het
Mical3	T	C	6: 121,010,748 (GRCm39)	I411M	probably damaging	Het
Myo5a	A	T	9: 75,124,789 (GRCm39)	K1781N	probably damaging	Het
N4bp2	T	C	5: 65,965,473 (GRCm39)	V1174A	probably benign	Het
Ncdn	A	T	4: 126,644,216 (GRCm39)	L202Q	probably damaging	Het
Ncor1	T	A	11: 62,283,464 (GRCm39)	M408L	possibly damaging	Het
Nf1	T	C	11: 79,300,235 (GRCm39)	L249P	probably damaging	Het
Nlrp9b	T	A	7: 19,783,521 (GRCm39)		probably null	Het
Nxpe3	A	T	16: 55,669,996 (GRCm39)	Y370N	probably damaging	Het
P2rx2	T	A	5: 110,489,434 (GRCm39)	T167S	probably benign	Het
Pcdhb19	G	T	18: 37,632,161 (GRCm39)	R652L	probably benign	Het
Pcdhga12	T	C	18: 37,901,334 (GRCm39)	L722P	probably benign	Het
Pde6a	A	T	18: 61,378,663 (GRCm39)	I329F	probably damaging	Het
Pdpr	T	A	8: 111,828,583 (GRCm39)	S29T	probably benign	Het
Pfkfb3	T	C	2: 11,491,123 (GRCm39)	D173G	probably benign	Het
Plcd4	A	G	1: 74,604,961 (GRCm39)		probably null	Het
Polr1g	T	A	7: 19,091,684 (GRCm39)	Q141L	probably damaging	Het
Pou2f2	A	G	7: 24,796,533 (GRCm39)		probably benign	Het
Ppp1r14c	TGGCGGCGGCGGCGGCGG	TGGCGGCGGCGGCGG	10: 3,316,702 (GRCm39)		probably benign	Het
Ppp1r42	T	C	1: 10,073,611 (GRCm39)		probably benign	Het
Ptpre	C	T	7: 135,270,861 (GRCm39)	H346Y	probably benign	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Rab12	A	T	17: 66,805,103 (GRCm39)	Y142N	probably damaging	Het
Rai14	A	C	15: 10,572,556 (GRCm39)	M857R	probably damaging	Het
Ranbp2	A	G	10: 58,328,243 (GRCm39)	K2753R	probably damaging	Het
Rasa4	A	T	5: 136,132,765 (GRCm39)	K6*	probably null	Het
Rasgef1a	A	G	6: 118,066,100 (GRCm39)	M438V	probably benign	Het
Recql	T	G	6: 142,304,732 (GRCm39)		probably benign	Het
Rftn2	A	G	1: 55,211,198 (GRCm39)	V425A	probably benign	Het
Ror1	A	G	4: 100,267,001 (GRCm39)	Y234C	probably damaging	Het
Sap30l	T	A	11: 57,696,880 (GRCm39)	L70Q	probably damaging	Het
Scn4a	T	C	11: 106,210,828 (GRCm39)	R1730G	probably damaging	Het
Serinc5	T	G	13: 92,827,488 (GRCm39)	I268R	probably damaging	Het
Sin3b	G	A	8: 73,471,576 (GRCm39)	V432I	possibly damaging	Het
Slc30a6	T	C	17: 74,719,649 (GRCm39)	M203T	possibly damaging	Het
Soat1	T	C	1: 156,259,898 (GRCm39)	N481S	probably damaging	Het
Sos2	G	A	12: 69,686,928 (GRCm39)	T206I	probably benign	Het
Sp100	G	T	1: 85,632,724 (GRCm39)	A132S	probably benign	Het
Sp140l1	C	T	1: 85,066,521 (GRCm39)		probably benign	Het
Spata31d1c	T	A	13: 65,183,604 (GRCm39)	L382*	probably null	Het
Stard9	T	A	2: 120,531,341 (GRCm39)	W2533R	probably benign	Het
Tas2r126	A	G	6: 42,412,324 (GRCm39)	T286A	probably benign	Het
Tedc2	T	A	17: 24,436,910 (GRCm39)	K275M	probably damaging	Het
Ten1	A	T	11: 116,109,057 (GRCm39)	K242N	probably benign	Het
Thoc2l	A	T	5: 104,665,616 (GRCm39)	D46V	possibly damaging	Het
Tial1	C	T	7: 128,056,752 (GRCm39)	V1I	probably damaging	Het
Tle2	G	A	10: 81,424,725 (GRCm39)	R649H	possibly damaging	Het
Tmem178	A	G	17: 81,252,374 (GRCm39)	D86G	probably benign	Het
Trim29	T	A	9: 43,240,872 (GRCm39)	D528E	possibly damaging	Het
Vmn2r61	A	T	7: 41,950,132 (GRCm39)	T851S	probably benign	Het
Vmn2r72	T	A	7: 85,399,806 (GRCm39)	Q414H	possibly damaging	Het
Yars1	A	T	4: 129,083,675 (GRCm39)		probably benign	Het
Yipf4	A	G	17: 74,801,087 (GRCm39)	Q135R	probably damaging	Het
Zfp341	G	A	2: 154,487,786 (GRCm39)		probably benign	Het
Zfp426	T	C	9: 20,381,334 (GRCm39)	Y536C	probably damaging	Het
Zp2	T	G	7: 119,734,995 (GRCm39)	Y430S	probably damaging	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,713,349 (GRCm39)	missense	probably benign
IGL00272:Lama3	APN	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,582,645 (GRCm39)	splice site	probably benign
IGL00836:Lama3	APN	18	12,605,285 (GRCm39)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,574,200 (GRCm39)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,614,094 (GRCm39)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,664,983 (GRCm39)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,574,188 (GRCm39)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,586,937 (GRCm39)	splice site	probably benign
IGL01863:Lama3	APN	18	12,552,993 (GRCm39)	splice site	probably benign
IGL01869:Lama3	APN	18	12,657,820 (GRCm39)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,705,121 (GRCm39)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,649,570 (GRCm39)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,601,371 (GRCm39)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,714,840 (GRCm39)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,624,533 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,689,807 (GRCm39)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,690,784 (GRCm39)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,670,915 (GRCm39)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,658,910 (GRCm39)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,711,184 (GRCm39)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,661,858 (GRCm39)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,601,425 (GRCm39)	splice site	probably benign
IGL03038:Lama3	APN	18	12,552,307 (GRCm39)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,572,406 (GRCm39)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,660,681 (GRCm39)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,614,095 (GRCm39)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,672,760 (GRCm39)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,686,340 (GRCm39)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,552,239 (GRCm39)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,686,288 (GRCm39)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,673,024 (GRCm39)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0106:Lama3	UTSW	18	12,537,039 (GRCm39)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,581,329 (GRCm39)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,657,867 (GRCm39)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0316:Lama3	UTSW	18	12,652,934 (GRCm39)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,615,183 (GRCm39)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,640,064 (GRCm39)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,540,620 (GRCm39)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,589,894 (GRCm39)	missense	probably benign
R0449:Lama3	UTSW	18	12,633,569 (GRCm39)	splice site	probably null
R0453:Lama3	UTSW	18	12,598,535 (GRCm39)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,583,481 (GRCm39)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,658,951 (GRCm39)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,694,758 (GRCm39)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,682,309 (GRCm39)	missense	probably benign
R0605:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,552,315 (GRCm39)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,552,302 (GRCm39)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,610,647 (GRCm39)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,589,907 (GRCm39)	splice site	probably benign
R1216:Lama3	UTSW	18	12,554,191 (GRCm39)	splice site	probably benign
R1356:Lama3	UTSW	18	12,633,634 (GRCm39)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,610,427 (GRCm39)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,653,048 (GRCm39)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,614,155 (GRCm39)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,682,284 (GRCm39)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,615,102 (GRCm39)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,646,788 (GRCm39)	splice site	probably benign
R1571:Lama3	UTSW	18	12,672,774 (GRCm39)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,583,457 (GRCm39)	nonsense	probably null
R1631:Lama3	UTSW	18	12,540,551 (GRCm39)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,612,929 (GRCm39)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,598,556 (GRCm39)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,535,119 (GRCm39)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,646,762 (GRCm39)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,670,838 (GRCm39)	nonsense	probably null
R1909:Lama3	UTSW	18	12,714,855 (GRCm39)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,628,336 (GRCm39)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,586,920 (GRCm39)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,657,778 (GRCm39)	splice site	probably benign
R2059:Lama3	UTSW	18	12,661,390 (GRCm39)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,661,783 (GRCm39)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,657,887 (GRCm39)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,535,906 (GRCm39)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,658,136 (GRCm39)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,546,915 (GRCm39)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,581,345 (GRCm39)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,572,532 (GRCm39)	splice site	probably benign
R3752:Lama3	UTSW	18	12,640,086 (GRCm39)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,637,365 (GRCm39)	nonsense	probably null
R4118:Lama3	UTSW	18	12,583,488 (GRCm39)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,646,747 (GRCm39)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,715,588 (GRCm39)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,652,929 (GRCm39)	nonsense	probably null
R4483:Lama3	UTSW	18	12,682,310 (GRCm39)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,614,145 (GRCm39)	missense	probably benign
R4516:Lama3	UTSW	18	12,628,415 (GRCm39)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,612,816 (GRCm39)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,637,454 (GRCm39)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,711,086 (GRCm39)	nonsense	probably null
R4704:Lama3	UTSW	18	12,686,280 (GRCm39)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,637,416 (GRCm39)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,615,141 (GRCm39)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,633,620 (GRCm39)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,546,828 (GRCm39)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,544,627 (GRCm39)	nonsense	probably null
R4784:Lama3	UTSW	18	12,582,601 (GRCm39)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,610,661 (GRCm39)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,574,188 (GRCm39)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,544,599 (GRCm39)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,631,735 (GRCm39)	intron	probably benign
R4953:Lama3	UTSW	18	12,581,362 (GRCm39)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,685,883 (GRCm39)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,651,800 (GRCm39)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,715,668 (GRCm39)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,665,005 (GRCm39)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,675,459 (GRCm39)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,672,823 (GRCm39)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,710,957 (GRCm39)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,552,950 (GRCm39)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,598,565 (GRCm39)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,685,912 (GRCm39)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,586,803 (GRCm39)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,705,123 (GRCm39)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,589,821 (GRCm39)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,686,267 (GRCm39)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,605,277 (GRCm39)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,572,405 (GRCm39)	missense	probably benign
R5617:Lama3	UTSW	18	12,631,993 (GRCm39)	intron	probably benign
R5709:Lama3	UTSW	18	12,672,856 (GRCm39)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,562,944 (GRCm39)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,707,311 (GRCm39)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,602,985 (GRCm39)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,615,156 (GRCm39)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,646,702 (GRCm39)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,657,794 (GRCm39)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,615,194 (GRCm39)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,612,813 (GRCm39)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,615,205 (GRCm39)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,628,405 (GRCm39)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,670,823 (GRCm39)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,710,897 (GRCm39)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,552,314 (GRCm39)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,646,735 (GRCm39)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,682,283 (GRCm39)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,661,475 (GRCm39)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,649,605 (GRCm39)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,715,602 (GRCm39)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,715,701 (GRCm39)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,685,870 (GRCm39)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,664,936 (GRCm39)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,595,839 (GRCm39)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,672,843 (GRCm39)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,601,346 (GRCm39)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,589,869 (GRCm39)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,537,133 (GRCm39)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,563,057 (GRCm39)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,715,665 (GRCm39)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,552,902 (GRCm39)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,572,449 (GRCm39)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,640,097 (GRCm39)	splice site	probably null
R7442:Lama3	UTSW	18	12,605,238 (GRCm39)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,552,294 (GRCm39)	missense	probably benign
R7604:Lama3	UTSW	18	12,633,550 (GRCm39)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,664,891 (GRCm39)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,670,895 (GRCm39)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,595,864 (GRCm39)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,670,796 (GRCm39)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,667,120 (GRCm39)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,639,999 (GRCm39)	missense	probably null
R8219:Lama3	UTSW	18	12,572,417 (GRCm39)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8229:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8298:Lama3	UTSW	18	12,658,910 (GRCm39)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,673,670 (GRCm39)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,661,404 (GRCm39)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,582,896 (GRCm39)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,544,688 (GRCm39)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,554,212 (GRCm39)	missense	probably benign
R8799:Lama3	UTSW	18	12,624,000 (GRCm39)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,582,643 (GRCm39)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,689,762 (GRCm39)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,665,096 (GRCm39)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,614,120 (GRCm39)	nonsense	probably null
R9126:Lama3	UTSW	18	12,583,527 (GRCm39)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,605,297 (GRCm39)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,595,869 (GRCm39)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,710,959 (GRCm39)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,583,541 (GRCm39)	nonsense	probably null
R9553:Lama3	UTSW	18	12,563,019 (GRCm39)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,682,320 (GRCm39)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,715,631 (GRCm39)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,562,936 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GCTGTTTCACTTTGAACAAAACGAG -3'
(R):5'- TGGCGCTCTCCATGTCATAG -3'

Sequencing Primer
(F):5'- TTTGAACAAAACGAGTTCTAAGGTGG -3'
(R):5'- TCTCCATGTCATAGACCCCAGGG -3'

Posted On

2016-03-17