Incidental Mutation 'R0282:Crk'
ID 37630
Institutional Source Beutler Lab
Gene Symbol Crk
Ensembl Gene ENSMUSG00000017776
Gene Name v-crk avian sarcoma virus CT10 oncogene homolog
Synonyms Crk-III, Crk-II, Crk-I, Crko, proto-oncogene c-crk
MMRRC Submission 038504-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0282 (G1)
Quality Score 225
Status Validated
Chromosome 11
Chromosomal Location 75570085-75597734 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 75594195 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Cysteine at position 261 (G261C)
Ref Sequence ENSEMBL: ENSMUSP00000017920 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000017920] [ENSMUST00000093115] [ENSMUST00000108425] [ENSMUST00000108426]
AlphaFold Q64010
PDB Structure CRK SH3 DOMAIN COMPLEXED WITH PEPTOID INHIBITOR [X-RAY DIFFRACTION]
STRUCTURAL BASIS FOR THE SPECIFIC INTERACTION OF LYSINE-CONTAINING PROLINE-RICH PEPTIDES WITH THE N-TERMINAL SH3 DOMAIN OF C-CRK [X-RAY DIFFRACTION]
STRUCTURAL BASIS FOR THE SPECIFIC INTERACTION OF LYSINE-CONTAINING PROLINE-RICH PEPTIDES WITH THE N-TERMINAL SH3 DOMAIN OF C-CRK [X-RAY DIFFRACTION]
Ternary complex of an Crk SH2 domain, Crk-derived phophopeptide, and Abl SH3 domain by NMR spectroscopy [SOLUTION NMR]
Solution structure of N-terminal SH3 domain from oncogene protein c-Crk [SOLUTION NMR]
Solution structure of a circular form of the truncated N-terminal SH3 domain from oncogene protein c-Crk. [SOLUTION NMR]
Solution structure of a circular form of the N-terminal SH3 domain (A134C, E135G, R191G mutant) from oncogene protein c-Crk. [SOLUTION NMR]
Solution structure of a circular form of the N-terminal SH3 domain (E132C, E133G, R191G mutant) from oncogene protein c-Crk [SOLUTION NMR]
Solution structure of the C-terminal SH3 domain of c-CrkII [SOLUTION NMR]
Predicted Effect probably damaging
Transcript: ENSMUST00000017920
AA Change: G261C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000017920
Gene: ENSMUSG00000017776
AA Change: G261C

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
SH3 238 295 6.86e-6 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000093115
SMART Domains Protein: ENSMUSP00000090803
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108425
AA Change: R204L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000104063
Gene: ENSMUSG00000017776
AA Change: R204L

DomainStartEndE-ValueType
SH2 11 110 4.98e-28 SMART
SH3 135 191 1.82e-19 SMART
Predicted Effect unknown
Transcript: ENSMUST00000108426
AA Change: R82L
SMART Domains Protein: ENSMUSP00000104064
Gene: ENSMUSG00000017776
AA Change: R82L

DomainStartEndE-ValueType
SH2 11 72 7.29e-12 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000147718
SMART Domains Protein: ENSMUSP00000116527
Gene: ENSMUSG00000017776

DomainStartEndE-ValueType
SH2 1 71 1.31e0 SMART
SH3 96 152 1.82e-19 SMART
Meta Mutation Damage Score 0.7554 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.4%
  • 10x: 96.6%
  • 20x: 93.7%
Validation Efficiency 100% (72/72)
MGI Phenotype FUNCTION: This gene is part of a family of adapter proteins that mediate formation of signal transduction complexes in response to extracellular stimuli, such as growth and differentiation factors. Protein-protein interactions occur through the SH2 domain, which binds phosphorylated tyrosine residues, and the SH3 domain, which binds proline-rich peptide motifs. These interactions promote recruitment and activation of effector proteins to regulate cell migration, adhesion, and proliferation. In mouse this protein is essential for embryonic development. Alternatively spliced transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for an isoform specific knockout do not exhibit any obvious abnormalities. Mice homozygous of a null allele of both isoforms exhibit fetal and perinatal lethality associated with abnormal cardiovascular morphology. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsbg3 T A 17: 57,192,169 (GRCm39) Y577* probably null Het
Afap1l2 A G 19: 56,904,653 (GRCm39) S549P possibly damaging Het
Alcam A T 16: 52,116,104 (GRCm39) C157S probably damaging Het
Aldh1a1 T C 19: 20,606,413 (GRCm39) probably benign Het
Ano8 G A 8: 71,933,258 (GRCm39) probably benign Het
Atr T C 9: 95,744,851 (GRCm39) V56A probably benign Het
Aurkc T A 7: 7,005,427 (GRCm39) probably null Het
Bnip3 T C 7: 138,499,759 (GRCm39) D76G probably damaging Het
Cbr1 A G 16: 93,407,022 (GRCm39) E246G possibly damaging Het
Ccdc157 G T 11: 4,096,708 (GRCm39) A449D probably damaging Het
Ces3b T A 8: 105,810,483 (GRCm39) V26D probably benign Het
Colgalt2 G T 1: 152,384,312 (GRCm39) A551S possibly damaging Het
Ctbp1 A G 5: 33,408,200 (GRCm39) probably null Het
Ctnna1 G A 18: 35,377,175 (GRCm39) V572I possibly damaging Het
D430041D05Rik T C 2: 104,031,589 (GRCm39) Y1669C probably damaging Het
Dnah8 T C 17: 30,955,130 (GRCm39) F2053S probably damaging Het
Dner G A 1: 84,383,686 (GRCm39) T566M probably damaging Het
Dner A G 1: 84,423,101 (GRCm39) probably benign Het
Edrf1 T C 7: 133,245,751 (GRCm39) V223A probably benign Het
Fam169a A G 13: 97,234,223 (GRCm39) probably benign Het
Fbxl3 G A 14: 103,332,661 (GRCm39) H106Y probably damaging Het
Fiz1 A G 7: 5,012,200 (GRCm39) V106A probably benign Het
Gapvd1 T A 2: 34,578,972 (GRCm39) R654* probably null Het
Gm7589 G A 9: 59,053,288 (GRCm39) noncoding transcript Het
Ifi202b A T 1: 173,804,926 (GRCm39) S9T probably benign Het
Ipmk G C 10: 71,208,661 (GRCm39) S149T probably benign Het
Irgm2 A G 11: 58,110,345 (GRCm39) E24G probably benign Het
Itga2b A C 11: 102,351,672 (GRCm39) V551G probably damaging Het
Itgad C T 7: 127,789,150 (GRCm39) probably benign Het
Kcnh8 T A 17: 53,032,879 (GRCm39) F55L probably damaging Het
Kdr G A 5: 76,110,760 (GRCm39) probably benign Het
Krt35 T C 11: 99,986,573 (GRCm39) Y147C probably damaging Het
Lamc1 A G 1: 153,131,058 (GRCm39) F298L probably benign Het
Lrrk2 T C 15: 91,662,617 (GRCm39) probably benign Het
Matn1 T C 4: 130,673,238 (GRCm39) S69P probably damaging Het
Micall1 G T 15: 79,016,101 (GRCm39) probably benign Het
Msto1 A G 3: 88,818,884 (GRCm39) V257A possibly damaging Het
Mybpc3 G C 2: 90,954,369 (GRCm39) probably benign Het
Mycn A G 12: 12,987,314 (GRCm39) V361A probably benign Het
Myo10 A G 15: 25,793,253 (GRCm39) T1277A probably damaging Het
Myo3a T C 2: 22,250,409 (GRCm39) I92T probably benign Het
Nup50l T C 6: 96,141,797 (GRCm39) T416A probably benign Het
Or10g1 A G 14: 52,647,720 (GRCm39) V203A possibly damaging Het
Or2k2 A G 4: 58,785,344 (GRCm39) I126T probably damaging Het
Or4c104 G A 2: 88,586,800 (GRCm39) T73I probably damaging Het
Or56b1b T A 7: 108,164,684 (GRCm39) Q106L probably damaging Het
Otog C T 7: 45,926,917 (GRCm39) T1222I possibly damaging Het
P4ha1 C T 10: 59,172,970 (GRCm39) T23M probably damaging Het
Pld1 A T 3: 28,132,422 (GRCm39) I537F probably benign Het
Plekhn1 A G 4: 156,312,780 (GRCm39) probably benign Het
Pxdn C A 12: 30,034,439 (GRCm39) S8* probably null Het
Rnf135 A T 11: 80,084,784 (GRCm39) I186F probably damaging Het
Rock2 T C 12: 17,027,887 (GRCm39) probably benign Het
Rph3a C A 5: 121,101,973 (GRCm39) G88* probably null Het
Sarm1 G A 11: 78,365,806 (GRCm39) Q740* probably null Het
Setd1b C A 5: 123,299,080 (GRCm39) probably benign Het
Sidt1 A G 16: 44,102,249 (GRCm39) S304P possibly damaging Het
Slc2a4 A T 11: 69,837,181 (GRCm39) V85E probably damaging Het
Swi5 A G 2: 32,170,766 (GRCm39) Y54H probably damaging Het
Sycp1 A G 3: 102,823,111 (GRCm39) probably benign Het
Tarm1 T C 7: 3,546,006 (GRCm39) Y87C probably damaging Het
Tmem67 G A 4: 12,087,930 (GRCm39) T72M probably damaging Het
Tor1a A G 2: 30,857,737 (GRCm39) Y44H possibly damaging Het
Ttll5 T C 12: 86,042,827 (GRCm39) Y1128H probably benign Het
Usp40 G A 1: 87,908,680 (GRCm39) probably benign Het
Vmn2r18 A T 5: 151,508,668 (GRCm39) M152K probably benign Het
Xirp2 T A 2: 67,343,724 (GRCm39) D1988E probably damaging Het
Zfp420 A G 7: 29,575,105 (GRCm39) I442V probably benign Het
Zyx A G 6: 42,332,939 (GRCm39) E363G probably damaging Het
Other mutations in Crk
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02223:Crk APN 11 75,594,205 (GRCm39) missense probably damaging 0.99
IGL02266:Crk APN 11 75,570,415 (GRCm39) missense probably damaging 1.00
R1956:Crk UTSW 11 75,583,496 (GRCm39) missense possibly damaging 0.83
R2864:Crk UTSW 11 75,594,211 (GRCm39) missense probably damaging 1.00
R5195:Crk UTSW 11 75,570,289 (GRCm39) missense probably damaging 1.00
R8556:Crk UTSW 11 75,583,347 (GRCm39) missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- CCAAGGAGGGCTAGGCTTTGAATC -3'
(R):5'- TGTTCCATCTGTCAGCAAACTGTCG -3'

Sequencing Primer
(F):5'- ATCCCCTAAGTCAGATGATGTGG -3'
(R):5'- GTCAGCAAACTGTCGAGCTATAC -3'
Posted On 2013-05-23