Mutagenetix > Incidental Mutation

Incidental Mutation 'R4869:Vps13d'

376395

Institutional Source

Beutler Lab

Gene Symbol

Vps13d

Ensembl Gene

ENSMUSG00000020220

Gene Name

vacuolar protein sorting 13D

Synonyms

MMRRC Submission

042479-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4869 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

144699192-144921575 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 144854612 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 2272 (L2272P)

Ref Sequence

ENSEMBL: ENSMUSP00000020441 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020441] [ENSMUST00000036579]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000020441
AA Change: L2272P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020441
Gene: ENSMUSG00000020220
AA Change: L2272P

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	118	1.8e-37	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
coiled coil region	665	685	N/A	INTRINSIC
low complexity region	765	781	N/A	INTRINSIC
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2866	2884	N/A	INTRINSIC
low complexity region	2973	2983	N/A	INTRINSIC
Pfam:DUF1162	3246	3530	1.1e-110	PFAM
low complexity region	3797	3810	N/A	INTRINSIC
low complexity region	3913	3921	N/A	INTRINSIC
low complexity region	4119	4132	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000036579
AA Change: L2278P

SMART Domains

Protein: ENSMUSP00000043240
Gene: ENSMUSG00000020220
AA Change: L2278P

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	116	3.5e-35	PFAM
Pfam:VPS13	131	353	9.6e-57	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
Pfam:VPS13_mid_rpt	608	896	4.3e-35	PFAM
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2891	2909	N/A	INTRINSIC
low complexity region	2998	3008	N/A	INTRINSIC
Pfam:SHR-BD	3271	3555	4.2e-86	PFAM
low complexity region	3822	3835	N/A	INTRINSIC
low complexity region	3938	3946	N/A	INTRINSIC
Pfam:VPS13_C	3978	4126	4.8e-24	PFAM
low complexity region	4144	4157	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000185113
AA Change: L1099P

Meta Mutation Damage Score

0.3449

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.3%

Validation Efficiency

99% (102/103)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	C	A	9: 57,161,488 (GRCm39)	K943N	probably damaging	Het
Abca13	G	A	11: 9,265,434 (GRCm39)		probably null	Het
Ankub1	A	G	3: 57,597,751 (GRCm39)	L73P	probably damaging	Het
Birc6	A	G	17: 74,893,007 (GRCm39)	I982V	probably benign	Het
Cabyr	G	T	18: 12,884,875 (GRCm39)	*454L	probably null	Het
Ccdc57	C	T	11: 120,794,344 (GRCm39)		probably null	Het
Cd209c	A	G	8: 3,994,077 (GRCm39)	F128L	probably benign	Het
Cdh23	A	T	10: 60,212,713 (GRCm39)	I1566N	probably damaging	Het
Cdon	T	C	9: 35,364,200 (GRCm39)	V106A	possibly damaging	Het
Cebpa	G	T	7: 34,819,246 (GRCm39)	G135C	probably damaging	Het
Cfap65	A	G	1: 74,958,420 (GRCm39)	S896P	probably benign	Het
Ciz1	T	C	2: 32,254,247 (GRCm39)	S63P	probably damaging	Het
Clybl	G	A	14: 122,621,618 (GRCm39)	V269M	probably damaging	Het
Ctnnb1	G	T	9: 120,782,060 (GRCm39)	V358L	possibly damaging	Het
Cwh43	G	T	5: 73,586,016 (GRCm39)		probably null	Het
Cyp2d10	G	A	15: 82,287,967 (GRCm39)	R379C	probably benign	Het
Dab2ip	C	A	2: 35,610,049 (GRCm39)	R727S	probably damaging	Het
Dmrt1	T	A	19: 25,483,219 (GRCm39)	M1K	probably null	Het
Dock1	A	T	7: 134,335,800 (GRCm39)	I65F	probably damaging	Het
Ercc2	T	C	7: 19,120,732 (GRCm39)	V155A	probably damaging	Het
Exph5	A	C	9: 53,287,539 (GRCm39)	D1540A	possibly damaging	Het
Fat3	T	A	9: 16,288,773 (GRCm39)	H250L	probably damaging	Het
Fbxw10	C	A	11: 62,753,557 (GRCm39)	A517E	probably damaging	Het
Furin	A	T	7: 80,046,727 (GRCm39)	N176K	probably damaging	Het
Gabrb3	A	G	7: 57,442,207 (GRCm39)		probably benign	Het
Gabrg2	A	T	11: 41,811,231 (GRCm39)	S305T	probably damaging	Het
Gas6	G	T	8: 13,525,086 (GRCm39)	S299R	possibly damaging	Het
Gga3	G	T	11: 115,477,111 (GRCm39)		probably benign	Het
Gle1	A	G	2: 29,826,032 (GRCm39)	E37G	possibly damaging	Het
Gm7964	A	G	7: 83,405,350 (GRCm39)	D80G	possibly damaging	Het
Gne	C	T	4: 44,055,204 (GRCm39)		probably null	Het
Grid2	G	T	6: 64,406,724 (GRCm39)	G695W	probably damaging	Het
H2-M10.6	A	T	17: 37,123,425 (GRCm39)	M40L	probably benign	Het
Hectd4	G	T	5: 121,460,735 (GRCm39)	V905L	possibly damaging	Het
Isyna1	C	A	8: 71,049,412 (GRCm39)	S441R	possibly damaging	Het
Kcnh3	C	T	15: 99,139,913 (GRCm39)	S933L	probably benign	Het
Knl1	T	C	2: 118,902,832 (GRCm39)	I1511T	possibly damaging	Het
Krt73	T	C	15: 101,704,833 (GRCm39)	E351G	probably damaging	Het
Lipo4	C	T	19: 33,478,953 (GRCm39)		probably null	Het
Llgl1	T	A	11: 60,598,036 (GRCm39)	L360*	probably null	Het
Map2k5	G	A	9: 63,229,525 (GRCm39)	R169*	probably null	Het
Muc19	A	T	15: 91,781,910 (GRCm39)		noncoding transcript	Het
Muc4	G	A	16: 32,754,836 (GRCm38)		probably benign	Het
Myh4	A	G	11: 67,143,490 (GRCm39)	E1074G	probably damaging	Het
Naip5	C	T	13: 100,381,639 (GRCm39)	G210E	probably damaging	Het
Nalcn	A	G	14: 123,837,296 (GRCm39)	S23P	probably benign	Het
Nepro	T	A	16: 44,550,536 (GRCm39)	M176K	probably damaging	Het
Nlrp6	G	A	7: 140,504,006 (GRCm39)	C704Y	probably damaging	Het
Notch1	A	G	2: 26,361,191 (GRCm39)	S1100P	probably benign	Het
Nrxn3	A	G	12: 88,762,352 (GRCm39)	E133G	possibly damaging	Het
Obi1	A	G	14: 104,716,252 (GRCm39)	I707T	probably damaging	Het
Parp9	T	C	16: 35,777,274 (GRCm39)	L406S	probably damaging	Het
Peak1	G	T	9: 56,134,876 (GRCm39)	A155D	probably benign	Het
Piezo1	G	A	8: 123,214,284 (GRCm39)	H1628Y	probably benign	Het
Piwil2	A	G	14: 70,632,811 (GRCm39)	V587A	probably benign	Het
Pkn2	A	G	3: 142,509,379 (GRCm39)	Y722H	probably damaging	Het
Plch2	A	T	4: 155,073,885 (GRCm39)	I834K	probably benign	Het
Pld1	A	G	3: 28,163,951 (GRCm39)	T795A	possibly damaging	Het
Plekhh1	A	G	12: 79,097,160 (GRCm39)	S103G	probably benign	Het
Ppl	T	C	16: 4,922,753 (GRCm39)	Y246C	probably damaging	Het
Prpf40b	G	A	15: 99,207,726 (GRCm39)		probably benign	Het
Prr14l	A	G	5: 32,986,177 (GRCm39)	L1106P	probably damaging	Het
Ptbp3	T	A	4: 59,524,443 (GRCm39)	I28F	possibly damaging	Het
Ptrh2	A	T	11: 86,580,631 (GRCm39)	K83*	probably null	Het
Rai1	T	C	11: 60,077,588 (GRCm39)	S551P	probably damaging	Het
Rapgefl1	A	T	11: 98,741,935 (GRCm39)	Q633L	probably damaging	Het
Rb1cc1	T	C	1: 6,285,245 (GRCm39)		probably benign	Het
Rif1	T	A	2: 51,983,623 (GRCm39)		probably benign	Het
Rnf141	A	G	7: 110,424,557 (GRCm39)	Y101H	probably damaging	Het
Rttn	T	A	18: 89,061,138 (GRCm39)	L1102*	probably null	Het
Sall4	G	A	2: 168,597,637 (GRCm39)	S401F	probably damaging	Het
Saxo5	G	A	8: 3,537,148 (GRCm39)	S498N	probably damaging	Het
Sec11c	T	C	18: 65,934,541 (GRCm39)	I36T	probably benign	Het
Sel1l	A	G	12: 91,780,828 (GRCm39)		probably benign	Het
Setd1a	T	C	7: 127,396,776 (GRCm39)		probably benign	Het
Sez6l2	C	T	7: 126,561,014 (GRCm39)	P433L	probably benign	Het
Sgf29	T	C	7: 126,248,547 (GRCm39)		probably benign	Het
Slc47a1	A	G	11: 61,253,520 (GRCm39)	V305A	probably benign	Het
Slco1a8	T	C	6: 141,933,492 (GRCm39)	D431G	probably damaging	Het
Sun2	T	C	15: 79,612,587 (GRCm39)		probably benign	Het
Tecta	T	C	9: 42,286,830 (GRCm39)	S609G	probably benign	Het
Tmprss6	T	A	15: 78,327,880 (GRCm39)		probably null	Het
Tns1	T	C	1: 73,991,774 (GRCm39)	H968R	probably benign	Het
Tomm34	G	A	2: 163,896,637 (GRCm39)	A270V	probably damaging	Het
Tpo	T	C	12: 30,153,364 (GRCm39)	K330R	probably benign	Het
Ttn	T	A	2: 76,542,837 (GRCm39)	Y33383F	probably damaging	Het
Unc13c	G	T	9: 73,587,716 (GRCm39)	A1439D	probably benign	Het
Vcp	C	T	4: 42,993,691 (GRCm39)	R147H	probably benign	Het
Wdfy3	A	G	5: 102,042,787 (GRCm39)	L1988P	probably damaging	Het
Wwc2	A	G	8: 48,373,713 (GRCm39)	F51S	probably damaging	Het
Zfp454	C	T	11: 50,763,980 (GRCm39)	C373Y	probably damaging	Het
Zfp853	A	T	5: 143,274,048 (GRCm39)	V473E	probably damaging	Het
Zfp959	A	T	17: 56,204,228 (GRCm39)	R85S	possibly damaging	Het

Other mutations in Vps13d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Vps13d	APN	4	144,895,110 (GRCm39)	missense	probably damaging	0.98
IGL00484:Vps13d	APN	4	144,853,145 (GRCm39)	missense	probably benign	0.04
IGL00591:Vps13d	APN	4	144,917,129 (GRCm39)	missense	possibly damaging	0.95
IGL00816:Vps13d	APN	4	144,882,564 (GRCm39)	missense	probably benign	0.00
IGL00835:Vps13d	APN	4	144,887,222 (GRCm39)	missense	probably damaging	0.97
IGL00847:Vps13d	APN	4	144,811,978 (GRCm39)	missense	probably benign	0.26
IGL01084:Vps13d	APN	4	144,881,525 (GRCm39)	missense	probably benign	0.00
IGL01116:Vps13d	APN	4	144,699,320 (GRCm39)	unclassified	probably benign
IGL01150:Vps13d	APN	4	144,875,845 (GRCm39)	missense	probably benign
IGL01329:Vps13d	APN	4	144,882,776 (GRCm39)	missense	possibly damaging	0.69
IGL01338:Vps13d	APN	4	144,814,892 (GRCm39)	missense	probably damaging	1.00
IGL01583:Vps13d	APN	4	144,771,658 (GRCm39)	missense	probably damaging	1.00
IGL01598:Vps13d	APN	4	144,743,471 (GRCm39)	missense	probably benign	0.21
IGL01620:Vps13d	APN	4	144,821,437 (GRCm39)	missense	possibly damaging	0.70
IGL01636:Vps13d	APN	4	144,801,618 (GRCm39)	missense	probably damaging	1.00
IGL01723:Vps13d	APN	4	144,899,715 (GRCm39)	missense	possibly damaging	0.84
IGL01895:Vps13d	APN	4	144,882,836 (GRCm39)	missense	possibly damaging	0.57
IGL01981:Vps13d	APN	4	144,813,317 (GRCm39)	missense	probably damaging	0.99
IGL02192:Vps13d	APN	4	144,875,428 (GRCm39)	missense	probably benign	0.02
IGL02197:Vps13d	APN	4	144,854,879 (GRCm39)	missense	probably benign	0.01
IGL02209:Vps13d	APN	4	144,882,671 (GRCm39)	missense	probably damaging	0.97
IGL02219:Vps13d	APN	4	144,894,716 (GRCm39)	missense	probably benign	0.00
IGL02377:Vps13d	APN	4	144,882,934 (GRCm39)	missense	probably damaging	1.00
IGL02404:Vps13d	APN	4	144,875,305 (GRCm39)	missense	probably damaging	1.00
IGL02552:Vps13d	APN	4	144,899,707 (GRCm39)	missense	possibly damaging	0.46
IGL02651:Vps13d	APN	4	144,891,129 (GRCm39)	missense	probably benign	0.02
IGL02708:Vps13d	APN	4	144,854,850 (GRCm39)	missense	probably benign	0.12
IGL02811:Vps13d	APN	4	144,858,335 (GRCm39)	missense	possibly damaging	0.55
IGL02821:Vps13d	APN	4	144,875,332 (GRCm39)	missense	probably damaging	0.98
IGL02838:Vps13d	APN	4	144,801,595 (GRCm39)	missense	probably benign	0.31
IGL02968:Vps13d	APN	4	144,849,068 (GRCm39)	missense	probably benign	0.32
IGL03176:Vps13d	APN	4	144,801,533 (GRCm39)	missense	probably benign	0.16
IGL03352:Vps13d	APN	4	144,894,072 (GRCm39)	missense	possibly damaging	0.49
IGL03374:Vps13d	APN	4	144,835,145 (GRCm39)	missense	possibly damaging	0.70
IGL03375:Vps13d	APN	4	144,818,517 (GRCm39)	missense	probably damaging	1.00
IGL03383:Vps13d	APN	4	144,894,889 (GRCm39)	critical splice acceptor site	probably null
IGL03411:Vps13d	APN	4	144,875,894 (GRCm39)	missense	probably damaging	1.00
broken	UTSW	4	144,813,305 (GRCm39)	missense
demotion	UTSW	4	144,865,183 (GRCm39)	missense
BB008:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
BB018:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
PIT4283001:Vps13d	UTSW	4	144,835,158 (GRCm39)	missense
PIT4434001:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0076:Vps13d	UTSW	4	144,891,264 (GRCm39)	splice site	probably benign
R0211:Vps13d	UTSW	4	144,841,348 (GRCm39)	missense	probably benign	0.08
R0219:Vps13d	UTSW	4	144,832,479 (GRCm39)	missense	probably benign	0.01
R0284:Vps13d	UTSW	4	144,871,372 (GRCm39)	missense	probably benign	0.01
R0345:Vps13d	UTSW	4	144,844,195 (GRCm39)	missense	possibly damaging	0.81
R0400:Vps13d	UTSW	4	144,792,397 (GRCm39)	missense	probably benign	0.00
R0417:Vps13d	UTSW	4	144,703,130 (GRCm39)	missense	probably benign	0.19
R0538:Vps13d	UTSW	4	144,771,665 (GRCm39)	missense	probably damaging	1.00
R0560:Vps13d	UTSW	4	144,780,760 (GRCm39)	missense	probably damaging	1.00
R0627:Vps13d	UTSW	4	144,813,754 (GRCm39)	missense	probably damaging	1.00
R0707:Vps13d	UTSW	4	144,882,502 (GRCm39)	missense	probably damaging	1.00
R0782:Vps13d	UTSW	4	144,853,195 (GRCm39)	splice site	probably benign
R0925:Vps13d	UTSW	4	144,883,121 (GRCm39)	missense	probably damaging	1.00
R0993:Vps13d	UTSW	4	144,844,262 (GRCm39)	nonsense	probably null
R1135:Vps13d	UTSW	4	144,882,159 (GRCm39)	missense	probably benign	0.01
R1165:Vps13d	UTSW	4	144,853,041 (GRCm39)	missense	probably benign
R1263:Vps13d	UTSW	4	144,896,918 (GRCm39)	missense	probably benign	0.01
R1397:Vps13d	UTSW	4	144,867,904 (GRCm39)	missense	probably damaging	1.00
R1398:Vps13d	UTSW	4	144,826,553 (GRCm39)	missense	probably null
R1521:Vps13d	UTSW	4	144,832,431 (GRCm39)	missense	probably benign	0.00
R1522:Vps13d	UTSW	4	144,824,742 (GRCm39)	splice site	probably null
R1725:Vps13d	UTSW	4	144,869,830 (GRCm39)	missense	possibly damaging	0.90
R1759:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1826:Vps13d	UTSW	4	144,881,573 (GRCm39)	missense	probably damaging	0.96
R1900:Vps13d	UTSW	4	144,853,176 (GRCm39)	missense	probably benign	0.23
R1943:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1955:Vps13d	UTSW	4	144,882,713 (GRCm39)	missense	probably damaging	1.00
R2008:Vps13d	UTSW	4	144,881,813 (GRCm39)	missense	probably benign	0.00
R2013:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2014:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2038:Vps13d	UTSW	4	144,907,685 (GRCm39)	critical splice donor site	probably null
R2108:Vps13d	UTSW	4	144,801,617 (GRCm39)	missense	probably damaging	0.99
R2130:Vps13d	UTSW	4	144,882,671 (GRCm39)	missense	probably benign	0.17
R2134:Vps13d	UTSW	4	144,874,909 (GRCm39)	missense	probably benign	0.00
R2168:Vps13d	UTSW	4	144,813,893 (GRCm39)	splice site	probably benign
R2220:Vps13d	UTSW	4	144,904,890 (GRCm39)	missense	probably damaging	1.00
R2240:Vps13d	UTSW	4	144,837,465 (GRCm39)	missense	possibly damaging	0.70
R2332:Vps13d	UTSW	4	144,875,256 (GRCm39)	missense	probably benign
R2357:Vps13d	UTSW	4	144,801,547 (GRCm39)	frame shift	probably null
R2365:Vps13d	UTSW	4	144,813,894 (GRCm39)	splice site	probably benign
R2571:Vps13d	UTSW	4	144,875,706 (GRCm39)	missense	probably benign	0.20
R3149:Vps13d	UTSW	4	144,853,147 (GRCm39)	missense	possibly damaging	0.70
R3150:Vps13d	UTSW	4	144,813,360 (GRCm39)	missense	probably damaging	0.98
R3547:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably damaging	0.99
R3716:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3718:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3725:Vps13d	UTSW	4	144,842,218 (GRCm39)	splice site	probably benign
R3794:Vps13d	UTSW	4	144,812,007 (GRCm39)	splice site	probably benign
R3875:Vps13d	UTSW	4	144,917,114 (GRCm39)	missense	probably damaging	1.00
R3948:Vps13d	UTSW	4	144,867,910 (GRCm39)	missense	probably damaging	1.00
R3953:Vps13d	UTSW	4	144,875,450 (GRCm39)	missense	probably damaging	1.00
R4021:Vps13d	UTSW	4	144,801,631 (GRCm39)	missense	possibly damaging	0.90
R4323:Vps13d	UTSW	4	144,879,348 (GRCm39)	missense	probably benign	0.28
R4346:Vps13d	UTSW	4	144,799,099 (GRCm39)	intron	probably benign
R4509:Vps13d	UTSW	4	144,789,172 (GRCm39)	missense	probably damaging	1.00
R4613:Vps13d	UTSW	4	144,858,225 (GRCm39)	missense	possibly damaging	0.95
R4657:Vps13d	UTSW	4	144,801,412 (GRCm39)	missense	probably damaging	1.00
R4680:Vps13d	UTSW	4	144,835,080 (GRCm39)	missense	possibly damaging	0.94
R4688:Vps13d	UTSW	4	144,904,782 (GRCm39)	missense	probably benign
R4797:Vps13d	UTSW	4	144,780,725 (GRCm39)	missense	probably damaging	1.00
R4798:Vps13d	UTSW	4	144,904,626 (GRCm39)	missense	probably damaging	0.98
R4817:Vps13d	UTSW	4	144,795,735 (GRCm39)	missense	probably damaging	1.00
R4839:Vps13d	UTSW	4	144,812,000 (GRCm39)	missense	possibly damaging	0.95
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4904:Vps13d	UTSW	4	144,882,015 (GRCm39)	missense	probably damaging	1.00
R4912:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R4916:Vps13d	UTSW	4	144,709,963 (GRCm39)	missense	probably damaging	1.00
R4976:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5029:Vps13d	UTSW	4	144,882,852 (GRCm39)	missense	probably benign	0.02
R5049:Vps13d	UTSW	4	144,813,336 (GRCm39)	missense	probably damaging	1.00
R5077:Vps13d	UTSW	4	144,814,811 (GRCm39)	missense	probably damaging	0.98
R5119:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5227:Vps13d	UTSW	4	144,907,777 (GRCm39)	splice site	probably null
R5291:Vps13d	UTSW	4	144,789,139 (GRCm39)	missense	probably damaging	0.99
R5344:Vps13d	UTSW	4	144,904,904 (GRCm39)	missense	probably damaging	0.98
R5348:Vps13d	UTSW	4	144,792,459 (GRCm39)	missense	probably damaging	0.99
R5478:Vps13d	UTSW	4	144,894,120 (GRCm39)	missense	probably damaging	0.99
R5632:Vps13d	UTSW	4	144,801,452 (GRCm39)	missense	probably damaging	0.99
R5642:Vps13d	UTSW	4	144,896,872 (GRCm39)	missense	possibly damaging	0.66
R5712:Vps13d	UTSW	4	144,813,743 (GRCm39)	missense	probably benign	0.07
R5747:Vps13d	UTSW	4	144,894,853 (GRCm39)	missense	probably benign	0.00
R5752:Vps13d	UTSW	4	144,875,540 (GRCm39)	missense	probably benign	0.06
R5804:Vps13d	UTSW	4	144,826,640 (GRCm39)	missense	probably benign	0.03
R5917:Vps13d	UTSW	4	144,826,580 (GRCm39)	missense	probably damaging	0.96
R5932:Vps13d	UTSW	4	144,771,611 (GRCm39)	missense	possibly damaging	0.71
R5940:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably benign	0.09
R5978:Vps13d	UTSW	4	144,849,181 (GRCm39)	missense	probably benign
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6143:Vps13d	UTSW	4	144,875,135 (GRCm39)	missense	possibly damaging	0.95
R6174:Vps13d	UTSW	4	144,701,763 (GRCm39)	nonsense	probably null
R6191:Vps13d	UTSW	4	144,875,918 (GRCm39)	missense	probably damaging	1.00
R6198:Vps13d	UTSW	4	144,875,560 (GRCm39)	missense	probably benign	0.28
R6374:Vps13d	UTSW	4	144,849,251 (GRCm39)	missense	probably damaging	1.00
R6379:Vps13d	UTSW	4	144,814,828 (GRCm39)	missense	probably benign
R6388:Vps13d	UTSW	4	144,882,144 (GRCm39)	missense	probably benign	0.06
R6418:Vps13d	UTSW	4	144,818,850 (GRCm39)	missense	probably damaging	0.98
R6466:Vps13d	UTSW	4	144,784,065 (GRCm39)	missense	possibly damaging	0.47
R6602:Vps13d	UTSW	4	144,830,234 (GRCm39)	intron	probably benign
R6604:Vps13d	UTSW	4	144,907,694 (GRCm39)	missense	probably damaging	1.00
R7051:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7052:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7103:Vps13d	UTSW	4	144,842,062 (GRCm39)	missense
R7231:Vps13d	UTSW	4	144,784,032 (GRCm39)	missense
R7246:Vps13d	UTSW	4	144,882,620 (GRCm39)	missense
R7339:Vps13d	UTSW	4	144,847,938 (GRCm39)	missense
R7409:Vps13d	UTSW	4	144,867,824 (GRCm39)	missense
R7419:Vps13d	UTSW	4	144,842,073 (GRCm39)	missense
R7424:Vps13d	UTSW	4	144,875,317 (GRCm39)	missense
R7439:Vps13d	UTSW	4	144,832,426 (GRCm39)	missense
R7440:Vps13d	UTSW	4	144,854,981 (GRCm39)	missense
R7528:Vps13d	UTSW	4	144,818,492 (GRCm39)	missense
R7547:Vps13d	UTSW	4	144,784,108 (GRCm39)	missense
R7558:Vps13d	UTSW	4	144,881,150 (GRCm39)	missense
R7699:Vps13d	UTSW	4	144,811,975 (GRCm39)	missense
R7729:Vps13d	UTSW	4	144,801,622 (GRCm39)	missense
R7789:Vps13d	UTSW	4	144,826,635 (GRCm39)	missense
R7813:Vps13d	UTSW	4	144,904,633 (GRCm39)	nonsense	probably null
R7834:Vps13d	UTSW	4	144,835,143 (GRCm39)	missense
R7840:Vps13d	UTSW	4	144,830,246 (GRCm39)	missense
R7880:Vps13d	UTSW	4	144,907,684 (GRCm39)	critical splice donor site	probably null
R7912:Vps13d	UTSW	4	144,899,697 (GRCm39)	missense
R7915:Vps13d	UTSW	4	144,813,389 (GRCm39)	missense
R7931:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
R8021:Vps13d	UTSW	4	144,875,245 (GRCm39)	missense
R8048:Vps13d	UTSW	4	144,882,137 (GRCm39)	missense
R8057:Vps13d	UTSW	4	144,701,753 (GRCm39)	missense
R8063:Vps13d	UTSW	4	144,841,327 (GRCm39)	missense
R8131:Vps13d	UTSW	4	144,882,707 (GRCm39)	missense
R8190:Vps13d	UTSW	4	144,879,321 (GRCm39)	missense
R8226:Vps13d	UTSW	4	144,875,860 (GRCm39)	missense
R8241:Vps13d	UTSW	4	144,875,047 (GRCm39)	missense
R8254:Vps13d	UTSW	4	144,709,882 (GRCm39)	splice site	probably benign
R8305:Vps13d	UTSW	4	144,818,858 (GRCm39)	missense
R8415:Vps13d	UTSW	4	144,818,549 (GRCm39)	missense
R8460:Vps13d	UTSW	4	144,897,009 (GRCm39)	intron	probably benign
R8487:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense	probably benign	0.11
R8543:Vps13d	UTSW	4	144,743,353 (GRCm39)	nonsense	probably null
R8679:Vps13d	UTSW	4	144,811,977 (GRCm39)	missense
R8716:Vps13d	UTSW	4	144,802,348 (GRCm39)	missense
R8749:Vps13d	UTSW	4	144,865,183 (GRCm39)	missense
R8772:Vps13d	UTSW	4	144,801,602 (GRCm39)	missense
R8788:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R8789:Vps13d	UTSW	4	144,795,743 (GRCm39)	missense
R8836:Vps13d	UTSW	4	144,882,648 (GRCm39)	missense
R8874:Vps13d	UTSW	4	144,881,772 (GRCm39)	missense
R8918:Vps13d	UTSW	4	144,772,873 (GRCm39)	missense
R9129:Vps13d	UTSW	4	144,898,249 (GRCm39)	missense
R9220:Vps13d	UTSW	4	144,783,058 (GRCm39)	missense
R9233:Vps13d	UTSW	4	144,879,344 (GRCm39)	missense
R9234:Vps13d	UTSW	4	144,875,792 (GRCm39)	missense
R9256:Vps13d	UTSW	4	144,882,374 (GRCm39)	missense
R9350:Vps13d	UTSW	4	144,882,333 (GRCm39)	missense
R9398:Vps13d	UTSW	4	144,896,956 (GRCm39)	nonsense	probably null
R9415:Vps13d	UTSW	4	144,796,527 (GRCm39)	missense
R9438:Vps13d	UTSW	4	144,858,314 (GRCm39)	missense
R9469:Vps13d	UTSW	4	144,780,691 (GRCm39)	missense
R9487:Vps13d	UTSW	4	144,807,869 (GRCm39)	critical splice donor site	probably null
R9524:Vps13d	UTSW	4	144,822,814 (GRCm39)	missense
R9616:Vps13d	UTSW	4	144,824,701 (GRCm39)	missense
R9655:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R9709:Vps13d	UTSW	4	144,875,915 (GRCm39)	missense
R9767:Vps13d	UTSW	4	144,879,306 (GRCm39)	missense
R9773:Vps13d	UTSW	4	144,818,619 (GRCm39)	missense
R9779:Vps13d	UTSW	4	144,798,972 (GRCm39)	missense
R9796:Vps13d	UTSW	4	144,854,505 (GRCm39)	critical splice donor site	probably null
X0021:Vps13d	UTSW	4	144,881,595 (GRCm39)	missense	probably damaging	0.99
Z1176:Vps13d	UTSW	4	144,833,637 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,904,866 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,881,478 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GTTAGTGAGACCATGCTCCAG -3'
(R):5'- AAGCTTGTAGTCTCGGCTGC -3'

Sequencing Primer
(F):5'- CTGAAGTGCTACCTACCTGAG -3'
(R):5'- TAGTCTCGGCTGCTGGGC -3'

Posted On

2016-03-17