Mutagenetix > Incidental Mutation

Incidental Mutation 'R4869:Wdfy3'

376399

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

2610509D04Rik, Ggtb3, Bchs, D5Ertd66e, Bwf1, Alfy

MMRRC Submission

042479-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.943) question?

Stock #

R4869 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

101980822-102217787 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 102042787 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 1988 (L1988P)

Ref Sequence

ENSEMBL: ENSMUSP00000134244 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000053177
AA Change: L1988P

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: L1988P

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

not run
Transcript: ENSMUST00000173385
AA Change: L43P

Predicted Effect

probably damaging
Transcript: ENSMUST00000174598
AA Change: L1988P

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: L1988P

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000212024
AA Change: L1973P

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

Meta Mutation Damage Score

0.2954

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.3%

Validation Efficiency

99% (102/103)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	C	A	9: 57,161,488 (GRCm39)	K943N	probably damaging	Het
Abca13	G	A	11: 9,265,434 (GRCm39)		probably null	Het
Ankub1	A	G	3: 57,597,751 (GRCm39)	L73P	probably damaging	Het
Birc6	A	G	17: 74,893,007 (GRCm39)	I982V	probably benign	Het
Cabyr	G	T	18: 12,884,875 (GRCm39)	*454L	probably null	Het
Ccdc57	C	T	11: 120,794,344 (GRCm39)		probably null	Het
Cd209c	A	G	8: 3,994,077 (GRCm39)	F128L	probably benign	Het
Cdh23	A	T	10: 60,212,713 (GRCm39)	I1566N	probably damaging	Het
Cdon	T	C	9: 35,364,200 (GRCm39)	V106A	possibly damaging	Het
Cebpa	G	T	7: 34,819,246 (GRCm39)	G135C	probably damaging	Het
Cfap65	A	G	1: 74,958,420 (GRCm39)	S896P	probably benign	Het
Ciz1	T	C	2: 32,254,247 (GRCm39)	S63P	probably damaging	Het
Clybl	G	A	14: 122,621,618 (GRCm39)	V269M	probably damaging	Het
Ctnnb1	G	T	9: 120,782,060 (GRCm39)	V358L	possibly damaging	Het
Cwh43	G	T	5: 73,586,016 (GRCm39)		probably null	Het
Cyp2d10	G	A	15: 82,287,967 (GRCm39)	R379C	probably benign	Het
Dab2ip	C	A	2: 35,610,049 (GRCm39)	R727S	probably damaging	Het
Dmrt1	T	A	19: 25,483,219 (GRCm39)	M1K	probably null	Het
Dock1	A	T	7: 134,335,800 (GRCm39)	I65F	probably damaging	Het
Ercc2	T	C	7: 19,120,732 (GRCm39)	V155A	probably damaging	Het
Exph5	A	C	9: 53,287,539 (GRCm39)	D1540A	possibly damaging	Het
Fat3	T	A	9: 16,288,773 (GRCm39)	H250L	probably damaging	Het
Fbxw10	C	A	11: 62,753,557 (GRCm39)	A517E	probably damaging	Het
Furin	A	T	7: 80,046,727 (GRCm39)	N176K	probably damaging	Het
Gabrb3	A	G	7: 57,442,207 (GRCm39)		probably benign	Het
Gabrg2	A	T	11: 41,811,231 (GRCm39)	S305T	probably damaging	Het
Gas6	G	T	8: 13,525,086 (GRCm39)	S299R	possibly damaging	Het
Gga3	G	T	11: 115,477,111 (GRCm39)		probably benign	Het
Gle1	A	G	2: 29,826,032 (GRCm39)	E37G	possibly damaging	Het
Gm7964	A	G	7: 83,405,350 (GRCm39)	D80G	possibly damaging	Het
Gne	C	T	4: 44,055,204 (GRCm39)		probably null	Het
Grid2	G	T	6: 64,406,724 (GRCm39)	G695W	probably damaging	Het
H2-M10.6	A	T	17: 37,123,425 (GRCm39)	M40L	probably benign	Het
Hectd4	G	T	5: 121,460,735 (GRCm39)	V905L	possibly damaging	Het
Isyna1	C	A	8: 71,049,412 (GRCm39)	S441R	possibly damaging	Het
Kcnh3	C	T	15: 99,139,913 (GRCm39)	S933L	probably benign	Het
Knl1	T	C	2: 118,902,832 (GRCm39)	I1511T	possibly damaging	Het
Krt73	T	C	15: 101,704,833 (GRCm39)	E351G	probably damaging	Het
Lipo4	C	T	19: 33,478,953 (GRCm39)		probably null	Het
Llgl1	T	A	11: 60,598,036 (GRCm39)	L360*	probably null	Het
Map2k5	G	A	9: 63,229,525 (GRCm39)	R169*	probably null	Het
Muc19	A	T	15: 91,781,910 (GRCm39)		noncoding transcript	Het
Muc4	G	A	16: 32,754,836 (GRCm38)		probably benign	Het
Myh4	A	G	11: 67,143,490 (GRCm39)	E1074G	probably damaging	Het
Naip5	C	T	13: 100,381,639 (GRCm39)	G210E	probably damaging	Het
Nalcn	A	G	14: 123,837,296 (GRCm39)	S23P	probably benign	Het
Nepro	T	A	16: 44,550,536 (GRCm39)	M176K	probably damaging	Het
Nlrp6	G	A	7: 140,504,006 (GRCm39)	C704Y	probably damaging	Het
Notch1	A	G	2: 26,361,191 (GRCm39)	S1100P	probably benign	Het
Nrxn3	A	G	12: 88,762,352 (GRCm39)	E133G	possibly damaging	Het
Obi1	A	G	14: 104,716,252 (GRCm39)	I707T	probably damaging	Het
Parp9	T	C	16: 35,777,274 (GRCm39)	L406S	probably damaging	Het
Peak1	G	T	9: 56,134,876 (GRCm39)	A155D	probably benign	Het
Piezo1	G	A	8: 123,214,284 (GRCm39)	H1628Y	probably benign	Het
Piwil2	A	G	14: 70,632,811 (GRCm39)	V587A	probably benign	Het
Pkn2	A	G	3: 142,509,379 (GRCm39)	Y722H	probably damaging	Het
Plch2	A	T	4: 155,073,885 (GRCm39)	I834K	probably benign	Het
Pld1	A	G	3: 28,163,951 (GRCm39)	T795A	possibly damaging	Het
Plekhh1	A	G	12: 79,097,160 (GRCm39)	S103G	probably benign	Het
Ppl	T	C	16: 4,922,753 (GRCm39)	Y246C	probably damaging	Het
Prpf40b	G	A	15: 99,207,726 (GRCm39)		probably benign	Het
Prr14l	A	G	5: 32,986,177 (GRCm39)	L1106P	probably damaging	Het
Ptbp3	T	A	4: 59,524,443 (GRCm39)	I28F	possibly damaging	Het
Ptrh2	A	T	11: 86,580,631 (GRCm39)	K83*	probably null	Het
Rai1	T	C	11: 60,077,588 (GRCm39)	S551P	probably damaging	Het
Rapgefl1	A	T	11: 98,741,935 (GRCm39)	Q633L	probably damaging	Het
Rb1cc1	T	C	1: 6,285,245 (GRCm39)		probably benign	Het
Rif1	T	A	2: 51,983,623 (GRCm39)		probably benign	Het
Rnf141	A	G	7: 110,424,557 (GRCm39)	Y101H	probably damaging	Het
Rttn	T	A	18: 89,061,138 (GRCm39)	L1102*	probably null	Het
Sall4	G	A	2: 168,597,637 (GRCm39)	S401F	probably damaging	Het
Saxo5	G	A	8: 3,537,148 (GRCm39)	S498N	probably damaging	Het
Sec11c	T	C	18: 65,934,541 (GRCm39)	I36T	probably benign	Het
Sel1l	A	G	12: 91,780,828 (GRCm39)		probably benign	Het
Setd1a	T	C	7: 127,396,776 (GRCm39)		probably benign	Het
Sez6l2	C	T	7: 126,561,014 (GRCm39)	P433L	probably benign	Het
Sgf29	T	C	7: 126,248,547 (GRCm39)		probably benign	Het
Slc47a1	A	G	11: 61,253,520 (GRCm39)	V305A	probably benign	Het
Slco1a8	T	C	6: 141,933,492 (GRCm39)	D431G	probably damaging	Het
Sun2	T	C	15: 79,612,587 (GRCm39)		probably benign	Het
Tecta	T	C	9: 42,286,830 (GRCm39)	S609G	probably benign	Het
Tmprss6	T	A	15: 78,327,880 (GRCm39)		probably null	Het
Tns1	T	C	1: 73,991,774 (GRCm39)	H968R	probably benign	Het
Tomm34	G	A	2: 163,896,637 (GRCm39)	A270V	probably damaging	Het
Tpo	T	C	12: 30,153,364 (GRCm39)	K330R	probably benign	Het
Ttn	T	A	2: 76,542,837 (GRCm39)	Y33383F	probably damaging	Het
Unc13c	G	T	9: 73,587,716 (GRCm39)	A1439D	probably benign	Het
Vcp	C	T	4: 42,993,691 (GRCm39)	R147H	probably benign	Het
Vps13d	A	G	4: 144,854,612 (GRCm39)	L2272P	probably damaging	Het
Wwc2	A	G	8: 48,373,713 (GRCm39)	F51S	probably damaging	Het
Zfp454	C	T	11: 50,763,980 (GRCm39)	C373Y	probably damaging	Het
Zfp853	A	T	5: 143,274,048 (GRCm39)	V473E	probably damaging	Het
Zfp959	A	T	17: 56,204,228 (GRCm39)	R85S	possibly damaging	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	102,063,204 (GRCm39)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	102,059,896 (GRCm39)	splice site	probably benign
IGL01288:Wdfy3	APN	5	102,049,857 (GRCm39)	splice site	probably null
IGL01323:Wdfy3	APN	5	102,042,930 (GRCm39)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	102,091,986 (GRCm39)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	102,047,897 (GRCm39)	missense	probably benign
IGL01560:Wdfy3	APN	5	102,105,352 (GRCm39)	nonsense	probably null
IGL01566:Wdfy3	APN	5	102,044,454 (GRCm39)	splice site	probably benign
IGL01616:Wdfy3	APN	5	102,061,126 (GRCm39)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	102,055,354 (GRCm39)	missense	probably benign
IGL01791:Wdfy3	APN	5	102,085,278 (GRCm39)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	102,071,947 (GRCm39)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	102,042,894 (GRCm39)	nonsense	probably null
IGL02121:Wdfy3	APN	5	102,046,376 (GRCm39)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	102,109,023 (GRCm39)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	102,070,475 (GRCm39)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	102,036,058 (GRCm39)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	102,044,341 (GRCm39)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	102,055,453 (GRCm39)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	102,116,786 (GRCm39)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	102,003,337 (GRCm39)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	102,042,778 (GRCm39)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	102,083,863 (GRCm39)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	102,014,142 (GRCm39)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,992,778 (GRCm39)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,992,465 (GRCm39)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	102,048,016 (GRCm39)	missense	probably damaging	1.00
Esurient	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	102,077,847 (GRCm39)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	102,030,827 (GRCm39)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0025:Wdfy3	UTSW	5	101,992,912 (GRCm39)	missense	probably damaging	0.98
R0031:Wdfy3	UTSW	5	102,037,161 (GRCm39)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,992,480 (GRCm39)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	102,035,971 (GRCm39)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	102,015,958 (GRCm39)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	102,096,832 (GRCm39)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	102,105,309 (GRCm39)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	102,038,655 (GRCm39)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	102,054,051 (GRCm39)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,984,038 (GRCm39)	missense	probably benign
R0787:Wdfy3	UTSW	5	102,105,254 (GRCm39)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	102,017,917 (GRCm39)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	102,030,832 (GRCm39)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	102,023,797 (GRCm39)	missense	probably benign
R1350:Wdfy3	UTSW	5	102,046,418 (GRCm39)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	102,032,080 (GRCm39)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,999,176 (GRCm39)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	102,085,604 (GRCm39)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	102,065,445 (GRCm39)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,991,947 (GRCm39)	missense	probably benign
R1633:Wdfy3	UTSW	5	102,129,414 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	102,023,781 (GRCm39)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	102,089,313 (GRCm39)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	102,074,391 (GRCm39)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,991,931 (GRCm39)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	102,096,795 (GRCm39)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	102,042,865 (GRCm39)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	102,063,242 (GRCm39)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	102,036,052 (GRCm39)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	102,065,301 (GRCm39)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	102,067,275 (GRCm39)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	102,099,178 (GRCm39)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	102,008,352 (GRCm39)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	102,042,926 (GRCm39)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	102,046,291 (GRCm39)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	102,055,408 (GRCm39)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	102,037,150 (GRCm39)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	102,036,189 (GRCm39)	splice site	probably benign
R2406:Wdfy3	UTSW	5	102,036,125 (GRCm39)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	102,023,796 (GRCm39)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	102,091,988 (GRCm39)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	102,009,266 (GRCm39)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	102,085,466 (GRCm39)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	102,092,105 (GRCm39)	nonsense	probably null
R3947:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	102,071,961 (GRCm39)	splice site	probably benign
R4065:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	102,047,924 (GRCm39)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	102,070,500 (GRCm39)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	102,058,850 (GRCm39)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	102,054,011 (GRCm39)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	102,031,949 (GRCm39)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	102,091,800 (GRCm39)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	102,077,894 (GRCm39)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	102,091,809 (GRCm39)	missense	probably damaging	1.00
R4971:Wdfy3	UTSW	5	102,096,838 (GRCm39)	nonsense	probably null
R4973:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	102,042,803 (GRCm39)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	102,015,972 (GRCm39)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,997,133 (GRCm39)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,994,972 (GRCm39)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	102,100,849 (GRCm39)	missense	probably damaging	1.00
R5368:Wdfy3	UTSW	5	102,020,724 (GRCm39)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	102,067,312 (GRCm39)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	102,044,425 (GRCm39)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,984,140 (GRCm39)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	102,009,314 (GRCm39)	missense	possibly damaging	0.69
R5877:Wdfy3	UTSW	5	102,017,855 (GRCm39)	missense	probably damaging	1.00
R5988:Wdfy3	UTSW	5	102,032,004 (GRCm39)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,999,225 (GRCm39)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,997,289 (GRCm39)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	102,046,295 (GRCm39)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	102,061,045 (GRCm39)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	102,101,032 (GRCm39)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	102,031,911 (GRCm39)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	102,065,297 (GRCm39)	nonsense	probably null
R6809:Wdfy3	UTSW	5	102,071,813 (GRCm39)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	102,100,865 (GRCm39)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,991,932 (GRCm39)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	102,042,775 (GRCm39)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	102,055,384 (GRCm39)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	102,063,303 (GRCm39)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	102,091,758 (GRCm39)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	102,049,785 (GRCm39)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,984,074 (GRCm39)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	102,003,389 (GRCm39)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	102,105,366 (GRCm39)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	102,030,354 (GRCm39)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	102,083,925 (GRCm39)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	102,003,252 (GRCm39)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,996,223 (GRCm39)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	102,099,265 (GRCm39)	nonsense	probably null
R7810:Wdfy3	UTSW	5	102,042,940 (GRCm39)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	102,089,476 (GRCm39)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	102,085,287 (GRCm39)	missense	probably benign
R8507:Wdfy3	UTSW	5	102,020,767 (GRCm39)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,999,219 (GRCm39)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	102,033,064 (GRCm39)	missense	probably benign
R8710:Wdfy3	UTSW	5	102,030,349 (GRCm39)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	102,077,951 (GRCm39)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	102,030,446 (GRCm39)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	102,065,421 (GRCm39)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,993,231 (GRCm39)	intron	probably benign
R8968:Wdfy3	UTSW	5	102,011,983 (GRCm39)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	102,096,764 (GRCm39)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,993,058 (GRCm39)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,995,040 (GRCm39)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	102,030,512 (GRCm39)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	102,091,831 (GRCm39)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	102,078,830 (GRCm39)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,996,359 (GRCm39)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	102,000,478 (GRCm39)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	102,078,716 (GRCm39)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	102,055,333 (GRCm39)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	102,100,957 (GRCm39)	missense
R9548:Wdfy3	UTSW	5	102,033,059 (GRCm39)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	102,047,899 (GRCm39)	missense	probably benign
R9750:Wdfy3	UTSW	5	102,077,960 (GRCm39)	missense	probably benign	0.00
R9766:Wdfy3	UTSW	5	102,042,866 (GRCm39)	missense	possibly damaging	0.90
R9771:Wdfy3	UTSW	5	102,000,195 (GRCm39)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	102,048,107 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- GTGTAACGTTAAAAGCCGTGC -3'
(R):5'- CTTCGCTAGGTGACAGATCTG -3'

Sequencing Primer
(F):5'- GTTAAAAGCCGTGCACCCTC -3'
(R):5'- CAGAAGAGTTTAAAGCCTTCGC -3'

Posted On

2016-03-17