Mutagenetix > Incidental Mutation

Incidental Mutation 'R4888:Micall1'

377147

Institutional Source

Beutler Lab

Gene Symbol

Micall1

Ensembl Gene

ENSMUSG00000033039

Gene Name

microtubule associated monooxygenase, calponin and LIM domain containing -like 1

Synonyms

Mus EST 820961, D15Mit260, D15N2e

MMRRC Submission

042493-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R4888 (G1)

Quality Score

216

Status

Validated

Chromosome

Chromosomal Location

78993098-79021100 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 79016048 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 802 (R802*)

Ref Sequence

ENSEMBL: ENSMUSP00000042053 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040320] [ENSMUST00000169604] [ENSMUST00000187550] [ENSMUST00000188562] [ENSMUST00000189761] [ENSMUST00000190959] [ENSMUST00000229031] [ENSMUST00000190730] [ENSMUST00000190509]

AlphaFold

Q8BGT6

Predicted Effect

probably null
Transcript: ENSMUST00000040320
AA Change: R802*

SMART Domains

Protein: ENSMUSP00000042053
Gene: ENSMUSG00000033039
AA Change: R802*

Domain	Start	End	E-Value	Type
CH	4	103	5.64e-19	SMART
low complexity region	113	135	N/A	INTRINSIC
LIM	164	219	1.15e-5	SMART
low complexity region	241	250	N/A	INTRINSIC
low complexity region	375	394	N/A	INTRINSIC
low complexity region	414	467	N/A	INTRINSIC
low complexity region	477	497	N/A	INTRINSIC
low complexity region	515	530	N/A	INTRINSIC
low complexity region	571	586	N/A	INTRINSIC
DUF3585	685	825	5.07e-68	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169604

SMART Domains

Protein: ENSMUSP00000129244
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	4	213	3.2e-92	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185944

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186559

Predicted Effect

probably benign
Transcript: ENSMUST00000187550

SMART Domains

Protein: ENSMUSP00000140978
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	1	57	2.1e-22	PFAM
Pfam:UPF0193	54	155	8.3e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188562

Predicted Effect

probably benign
Transcript: ENSMUST00000189761

SMART Domains

Protein: ENSMUSP00000139736
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	1	39	4.9e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000190959

SMART Domains

Protein: ENSMUSP00000140347
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	1	216	1.5e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000229031

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000230202

Predicted Effect

probably benign
Transcript: ENSMUST00000190730

SMART Domains

Protein: ENSMUSP00000139884
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	1	57	2.1e-22	PFAM
Pfam:UPF0193	54	155	8.3e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000190509

SMART Domains

Protein: ENSMUSP00000140611
Gene: ENSMUSG00000033029

Domain	Start	End	E-Value	Type
Pfam:UPF0193	33	195	4.4e-46	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000230779

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000229694

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

99% (150/151)

Allele List at MGI

Other mutations in this stock

Total: 139 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931403M11Rik	C	A	14: 24,671,419 (GRCm39)		noncoding transcript	Het
Abca7	A	G	10: 79,838,562 (GRCm39)	K612R	probably damaging	Het
Adamtsl3	T	C	7: 82,223,822 (GRCm39)	V275A	possibly damaging	Het
Afg3l1	G	A	8: 124,215,065 (GRCm39)		probably null	Het
Ajm1	G	A	2: 25,469,759 (GRCm39)	R51C	possibly damaging	Het
Alcam	T	A	16: 52,089,176 (GRCm39)	H508L	probably benign	Het
Ankrd45	G	A	1: 160,982,942 (GRCm39)	R151H	probably damaging	Het
Aqr	G	A	2: 113,980,990 (GRCm39)	L264F	probably damaging	Het
Arfgef2	G	A	2: 166,677,533 (GRCm39)	A53T	probably damaging	Het
Arhgap30	A	G	1: 171,236,880 (GRCm39)	T1085A	probably benign	Het
Atp10a	T	C	7: 58,435,055 (GRCm39)	V314A	probably damaging	Het
Bdp1	T	C	13: 100,187,627 (GRCm39)	D1580G	probably benign	Het
Btbd17	T	A	11: 114,684,917 (GRCm39)	T41S	possibly damaging	Het
Cacna1c	T	C	6: 118,728,400 (GRCm39)	N378S	probably damaging	Het
Camsap1	G	T	2: 25,825,562 (GRCm39)	Q1375K	probably benign	Het
Catsper2	G	T	2: 121,227,604 (GRCm39)		probably null	Het
Cblif	A	G	19: 11,729,583 (GRCm39)	T181A	probably benign	Het
Ccar1	A	G	10: 62,588,997 (GRCm39)	S829P	unknown	Het
Ccdc180	T	C	4: 45,909,308 (GRCm39)	L492P	probably damaging	Het
Ccser2	A	T	14: 36,662,343 (GRCm39)	N280K	probably damaging	Het
Cd37	T	C	7: 44,883,359 (GRCm39)	D271G	probably damaging	Het
Cdcp1	G	A	9: 123,011,194 (GRCm39)		probably benign	Het
Cemip2	T	C	19: 21,833,528 (GRCm39)	V1322A	probably benign	Het
Cep85	T	A	4: 133,892,062 (GRCm39)		probably benign	Het
Cfi	C	T	3: 129,666,726 (GRCm39)	P483L	probably damaging	Het
Csmd1	A	G	8: 15,945,674 (GRCm39)		probably benign	Het
Cytl1	G	T	5: 37,892,918 (GRCm39)	L9F	unknown	Het
Defb26	T	C	2: 152,350,085 (GRCm39)	E65G	possibly damaging	Het
Degs1	G	A	1: 182,104,370 (GRCm39)	T305I	probably damaging	Het
Dnttip2	T	G	3: 122,070,241 (GRCm39)	C485W	probably damaging	Het
Ecel1	G	A	1: 87,076,449 (GRCm39)		probably benign	Het
Edrf1	G	T	7: 133,260,339 (GRCm39)	M83I	probably damaging	Het
Elmo2	A	T	2: 165,137,209 (GRCm39)	C562S	probably benign	Het
Etl4	T	A	2: 20,344,922 (GRCm39)		probably null	Het
F2r	A	C	13: 95,740,905 (GRCm39)	V210G	probably damaging	Het
Fam171a1	T	A	2: 3,224,546 (GRCm39)	V179E	probably damaging	Het
Fmod	G	T	1: 133,967,977 (GRCm39)	V6F	possibly damaging	Het
Foxr2	A	G	X: 151,913,312 (GRCm39)	Q61R	probably damaging	Het
Gabra4	G	A	5: 71,729,546 (GRCm39)	H76Y	probably benign	Het
Gck	A	T	11: 5,859,150 (GRCm39)	M139K	possibly damaging	Het
Gm10192	C	G	4: 97,071,345 (GRCm39)	S20T	probably null	Het
Gm44501	T	C	17: 40,887,515 (GRCm39)	L11P	unknown	Het
Gm572	T	C	4: 148,751,302 (GRCm39)		probably null	Het
Gnas	A	G	2: 174,139,882 (GRCm39)	K17R	possibly damaging	Het
Grm2	T	A	9: 106,527,865 (GRCm39)	S340C	probably damaging	Het
Gsc	T	A	12: 104,438,274 (GRCm39)	H198L	probably damaging	Het
H2-Q3	A	T	17: 35,578,424 (GRCm39)		noncoding transcript	Het
H4c1	T	C	13: 23,944,935 (GRCm39)	D69G	probably damaging	Het
Hivep3	A	G	4: 119,980,131 (GRCm39)	E1723G	probably damaging	Het
Hk3	A	T	13: 55,154,405 (GRCm39)	L757Q	probably damaging	Het
Homer2	A	T	7: 81,299,311 (GRCm39)	D51E	probably benign	Het
Ier3ip1	T	A	18: 77,027,227 (GRCm39)		probably benign	Het
Itch	T	A	2: 155,059,897 (GRCm39)		probably null	Het
Kalrn	T	A	16: 33,991,700 (GRCm39)	Q974L	probably damaging	Het
Kirrel3	G	T	9: 34,924,601 (GRCm39)	G296W	probably damaging	Het
Klhl17	A	G	4: 156,315,082 (GRCm39)	V525A	probably benign	Het
Klhl9	T	C	4: 88,640,182 (GRCm39)	K20E	probably benign	Het
Kmt2a	G	T	9: 44,732,977 (GRCm39)		probably benign	Het
Krt17	C	A	11: 100,147,305 (GRCm39)	V409L	probably benign	Het
Lmx1b	T	C	2: 33,454,802 (GRCm39)	N322S	probably benign	Het
Lrrc63	G	C	14: 75,363,406 (GRCm39)	P242A	probably benign	Het
Ltk	T	C	2: 119,583,708 (GRCm39)	N256D	probably damaging	Het
Map3k19	T	A	1: 127,745,470 (GRCm39)	E1328V	probably damaging	Het
Map4k2	C	T	19: 6,394,033 (GRCm39)	A280V	probably benign	Het
Mmp14	A	G	14: 54,673,662 (GRCm39)	R109G	probably damaging	Het
Mmp27	A	T	9: 7,581,369 (GRCm39)	H544L	probably benign	Het
Mpeg1	A	G	19: 12,440,434 (GRCm39)	T631A	probably damaging	Het
Mpnd	A	G	17: 56,318,641 (GRCm39)	M217V	probably benign	Het
Mrc2	T	C	11: 105,232,034 (GRCm39)	V862A	probably damaging	Het
Mug2	T	A	6: 122,058,154 (GRCm39)	S1239T	probably damaging	Het
Myb	T	A	10: 21,002,137 (GRCm39)	M616L	probably benign	Het
Myo18b	A	T	5: 113,022,346 (GRCm39)		probably benign	Het
Myo1c	C	A	11: 75,560,053 (GRCm39)	T703N	probably damaging	Het
Myt1l	T	G	12: 29,936,138 (GRCm39)	C909G	unknown	Het
Nbea	T	C	3: 55,912,776 (GRCm39)	S1004G	possibly damaging	Het
Neb	T	C	2: 52,186,319 (GRCm39)	N918D	probably benign	Het
Nrtn	T	C	17: 57,058,636 (GRCm39)	T122A	probably damaging	Het
Nsd3	A	T	8: 26,188,939 (GRCm39)	S1042C	probably damaging	Het
Ntmt2	A	T	1: 163,530,633 (GRCm39)	C269S	probably benign	Het
Nvl	A	G	1: 180,945,191 (GRCm39)	L461S	probably damaging	Het
Onecut2	A	T	18: 64,473,998 (GRCm39)	H183L	possibly damaging	Het
Or13p5	A	C	4: 118,591,885 (GRCm39)	H53P	probably benign	Het
Or2h2	G	A	17: 37,396,343 (GRCm39)	T238I	probably damaging	Het
Or4d10	C	G	19: 12,051,679 (GRCm39)	G106R	probably damaging	Het
Or52e4	A	G	7: 104,705,689 (GRCm39)	T79A	possibly damaging	Het
Or5c1	T	G	2: 37,222,601 (GRCm39)	F281V	probably damaging	Het
Or6c1b	A	C	10: 129,273,248 (GRCm39)	D189A	possibly damaging	Het
Or6z5	A	T	7: 6,477,612 (GRCm39)	I168F	probably damaging	Het
Pcdhb21	G	T	18: 37,648,130 (GRCm39)	V420F	possibly damaging	Het
Pcdhb9	G	A	18: 37,536,286 (GRCm39)	G760D	probably benign	Het
Pglyrp2	G	A	17: 32,637,771 (GRCm39)	P86S	probably benign	Het
Phyhip	T	A	14: 70,704,765 (GRCm39)	V328E	probably damaging	Het
Pitpnb	A	T	5: 111,530,862 (GRCm39)	K218N	possibly damaging	Het
Pitrm1	A	G	13: 6,628,596 (GRCm39)	D963G	probably damaging	Het
Pmfbp1	A	G	8: 110,258,792 (GRCm39)	Y699C	probably damaging	Het
Pmpcb	T	A	5: 21,945,660 (GRCm39)		probably benign	Het
Polg	A	T	7: 79,114,353 (GRCm39)	W203R	probably damaging	Het
Pramel22	A	G	4: 143,380,971 (GRCm39)	S351P	probably benign	Het
Prss41	A	T	17: 24,055,977 (GRCm39)	M252K	probably benign	Het
Prss46	A	T	9: 110,673,618 (GRCm39)	M1L	possibly damaging	Het
Rimklb	T	C	6: 122,441,051 (GRCm39)		probably null	Het
Rngtt	T	C	4: 33,500,335 (GRCm39)	I556T	unknown	Het
Rock1	A	C	18: 10,122,698 (GRCm39)	D374E	probably benign	Het
Rspry1	C	A	8: 95,385,417 (GRCm39)	H450N	probably benign	Het
Rusc2	T	C	4: 43,423,942 (GRCm39)	V1032A	probably damaging	Het
Sacs	A	G	14: 61,449,647 (GRCm39)	R3898G	probably damaging	Het
Scnn1b	A	T	7: 121,502,110 (GRCm39)	H256L	probably benign	Het
Sh3gl2	T	A	4: 85,297,494 (GRCm39)	H157Q	probably benign	Het
Sipa1l1	C	A	12: 82,389,107 (GRCm39)	S444R	probably damaging	Het
Sntg1	T	A	1: 8,433,818 (GRCm39)	I478F	probably damaging	Het
Spata31e2	A	G	1: 26,722,628 (GRCm39)	Y851H	probably benign	Het
Sptb	G	T	12: 76,655,811 (GRCm39)	L1424M	probably benign	Het
Sry	T	A	Y: 2,663,105 (GRCm39)	H185L	unknown	Het
Stox2	T	G	8: 47,656,198 (GRCm39)	T93P	probably damaging	Het
Tatdn2	T	A	6: 113,681,566 (GRCm39)	F472Y	possibly damaging	Het
Tefm	A	T	11: 80,031,185 (GRCm39)	V17D	possibly damaging	Het
Tekt2	G	T	4: 126,218,460 (GRCm39)	T70N	probably benign	Het
Ticam1	T	C	17: 56,578,642 (GRCm39)	D151G	probably damaging	Het
Tmem63c	A	G	12: 87,136,139 (GRCm39)	E785G	probably damaging	Het
Tnmd	A	T	X: 132,765,551 (GRCm39)	N219I	probably benign	Het
Ttc14	T	A	3: 33,861,024 (GRCm39)	L407*	probably null	Het
Ttll7	T	C	3: 146,599,932 (GRCm39)	M1T	probably null	Het
Ttn	T	A	2: 76,746,835 (GRCm39)		probably null	Het
Uchl1	T	C	5: 66,833,780 (GRCm39)	M12T	probably benign	Het
Uggt2	A	T	14: 119,315,062 (GRCm39)		probably null	Het
Uggt2	T	C	14: 119,286,665 (GRCm39)	T224A	probably damaging	Het
Umodl1	A	G	17: 31,218,175 (GRCm39)	E1254G	probably damaging	Het
Unc5d	A	G	8: 29,156,927 (GRCm39)	F773L	probably benign	Het
Unc80	C	A	1: 66,683,606 (GRCm39)	P2216Q	probably damaging	Het
Usp40	T	A	1: 87,913,923 (GRCm39)		probably null	Het
Vmn1r117	A	T	7: 20,617,421 (GRCm39)	M209K	probably damaging	Het
Vmn2r44	A	T	7: 8,380,985 (GRCm39)	W303R	probably benign	Het
Wnk2	G	A	13: 49,224,478 (GRCm39)	R268C	probably damaging	Het
Wnt2	T	C	6: 18,023,125 (GRCm39)	K175E	possibly damaging	Het
Xkr6	T	C	14: 64,056,953 (GRCm39)	V288A	unknown	Het
Zfp125	A	G	12: 20,950,459 (GRCm39)		noncoding transcript	Het
Zfp512b	A	C	2: 181,228,856 (GRCm39)	V703G	probably damaging	Het
Zglp1	A	T	9: 20,973,957 (GRCm39)	I243N	probably benign	Het
Zgpat	T	C	2: 181,020,651 (GRCm39)	S275P	probably benign	Het

Other mutations in Micall1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00496:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00508:Micall1	APN	15	79,014,768 (GRCm39)	missense	probably damaging	0.99
IGL01626:Micall1	APN	15	79,014,712 (GRCm39)	missense	possibly damaging	0.46
IGL01868:Micall1	APN	15	78,999,260 (GRCm39)	missense	probably benign	0.41
IGL03062:Micall1	APN	15	78,998,881 (GRCm39)	missense	probably damaging	1.00
R0086:Micall1	UTSW	15	79,009,689 (GRCm39)	utr 3 prime	probably benign
R0099:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0282:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0727:Micall1	UTSW	15	79,004,978 (GRCm39)	missense	probably benign	0.00
R1859:Micall1	UTSW	15	79,007,145 (GRCm39)	intron	probably benign
R2142:Micall1	UTSW	15	79,014,995 (GRCm39)	missense	probably damaging	0.98
R2228:Micall1	UTSW	15	79,014,036 (GRCm39)	missense	probably damaging	1.00
R3508:Micall1	UTSW	15	79,006,965 (GRCm39)	missense	probably damaging	1.00
R4858:Micall1	UTSW	15	79,007,146 (GRCm39)	intron	probably benign
R5059:Micall1	UTSW	15	79,007,034 (GRCm39)	intron	probably benign
R5097:Micall1	UTSW	15	79,014,078 (GRCm39)	missense	probably benign	0.17
R5451:Micall1	UTSW	15	79,011,104 (GRCm39)	splice site	probably null
R5710:Micall1	UTSW	15	79,011,290 (GRCm39)	missense	probably damaging	1.00
R5727:Micall1	UTSW	15	79,014,678 (GRCm39)	missense	possibly damaging	0.64
R7135:Micall1	UTSW	15	78,993,624 (GRCm39)	missense	unknown
R7186:Micall1	UTSW	15	79,009,575 (GRCm39)	missense	unknown
R7297:Micall1	UTSW	15	79,005,097 (GRCm39)	missense	unknown
R7472:Micall1	UTSW	15	79,006,760 (GRCm39)	missense	unknown
R8494:Micall1	UTSW	15	79,005,080 (GRCm39)	missense	probably damaging	1.00
R8714:Micall1	UTSW	15	79,011,510 (GRCm39)	missense	probably benign	0.03
R8937:Micall1	UTSW	15	79,011,198 (GRCm39)	missense	probably damaging	1.00
R9440:Micall1	UTSW	15	79,011,159 (GRCm39)	missense
R9760:Micall1	UTSW	15	79,005,032 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CCCAAGTCACAGAATGTCCTTG -3'
(R):5'- GAGAAGCTGAGTGTCCACAG -3'

Sequencing Primer
(F):5'- AAGTCACAGAATGTCCTTGGCTCC -3'
(R):5'- CTGAGTGTCCACAGCAGGAAG -3'

Posted On

2016-03-17