Mutagenetix > Incidental Mutation

Incidental Mutation 'R4890:Or8c15'

377268

Institutional Source

Beutler Lab

Gene Symbol

Or8c15

Ensembl Gene

ENSMUSG00000093901

Gene Name

olfactory receptor family 8 subfamily C member 15

Synonyms

GA_x6K02T2PVTD-31889215-31890153, Olfr893, MOR170-11

MMRRC Submission

042495-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.168) question?

Stock #

R4890 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38120348-38121289 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 38120586 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Phenylalanine at position 79 (C79F)

Ref Sequence

ENSEMBL: ENSMUSP00000091390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093867] [ENSMUST00000211975]

AlphaFold

K7N678

Predicted Effect

probably benign
Transcript: ENSMUST00000093867
AA Change: C79F

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000091390
Gene: ENSMUSG00000093901
AA Change: C79F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	309	1.2e-45	PFAM
Pfam:7tm_1	43	292	8e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000211975
AA Change: C77F

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

unknown
Transcript: ENSMUST00000212815
AA Change: C26F

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.4%

Validation Efficiency

99% (81/82)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
AA986860	T	C	1: 130,668,725 (GRCm39)		probably benign	Het
Adcy4	C	T	14: 56,016,486 (GRCm39)	D322N	probably damaging	Het
Adgrb3	T	C	1: 25,260,908 (GRCm39)	N916S	probably damaging	Het
Aox1	T	C	1: 58,373,862 (GRCm39)	V841A	probably benign	Het
Baz2b	A	T	2: 59,756,383 (GRCm39)	M983K	probably damaging	Het
C2cd2l	A	G	9: 44,222,430 (GRCm39)	F682L	probably damaging	Het
Ccsap	T	G	8: 124,572,160 (GRCm39)	E114A	possibly damaging	Het
Cept1	T	A	3: 106,413,123 (GRCm39)	T201S	probably damaging	Het
Cfap221	T	C	1: 119,883,476 (GRCm39)	M232V	probably benign	Het
Chsy1	A	G	7: 65,759,974 (GRCm39)	R106G	probably benign	Het
Cit	C	T	5: 116,126,182 (GRCm39)		probably benign	Het
Cldn7	G	A	11: 69,857,918 (GRCm39)	V42I	probably benign	Het
Cnnm4	T	A	1: 36,511,345 (GRCm39)	V191E	probably benign	Het
Cntf	A	T	19: 12,741,326 (GRCm39)	V178D	possibly damaging	Het
Ctsz	C	A	2: 174,270,393 (GRCm39)	R263L	probably damaging	Het
Dclk1	T	C	3: 55,429,353 (GRCm39)	M407T	probably benign	Het
Dennd1a	A	T	2: 38,066,238 (GRCm39)		probably benign	Het
Dnhd1	A	G	7: 105,306,164 (GRCm39)	I368V	possibly damaging	Het
Gak	A	T	5: 108,728,742 (GRCm39)		probably benign	Het
Hepacam2	A	T	6: 3,487,231 (GRCm39)	V42D	probably damaging	Het
Il1rl2	CTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATT	CTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATTTTATT	1: 40,366,470 (GRCm39)		probably benign	Het
Insr	T	A	8: 3,248,234 (GRCm39)	Q437L	probably benign	Het
Itga8	G	A	2: 12,198,102 (GRCm39)		probably benign	Het
Kansl1	A	T	11: 104,233,868 (GRCm39)	C732S	probably benign	Het
Kdsr	T	C	1: 106,680,964 (GRCm39)	K78R	probably benign	Het
Kif14	T	C	1: 136,414,868 (GRCm39)	S785P	possibly damaging	Het
Lbr	C	A	1: 181,645,133 (GRCm39)	L506F	probably benign	Het
Macf1	C	A	4: 123,342,031 (GRCm39)	C2720F	probably damaging	Het
Mapt	G	A	11: 104,218,975 (GRCm39)	D738N	probably damaging	Het
Mroh9	T	C	1: 162,854,093 (GRCm39)	Y769C	probably damaging	Het
Mylk2	A	G	2: 152,762,274 (GRCm39)	N515S	possibly damaging	Het
Myorg	G	A	4: 41,498,877 (GRCm39)	T251M	probably benign	Het
Nek10	T	A	14: 14,860,986 (GRCm38)	L513M	possibly damaging	Het
Nrxn2	A	T	19: 6,498,308 (GRCm39)	S258C	possibly damaging	Het
Nudt16l2	A	T	9: 105,021,786 (GRCm39)	S87T	possibly damaging	Het
Or52e15	G	A	7: 104,645,311 (GRCm39)	H267Y	probably benign	Het
Or5m12	C	T	2: 85,735,092 (GRCm39)	C102Y	possibly damaging	Het
Or6c1b	C	T	10: 129,272,948 (GRCm39)	T89I	probably benign	Het
Or6z5	T	A	7: 6,477,848 (GRCm39)	C246*	probably null	Het
Osgin2	G	A	4: 16,013,739 (GRCm39)		probably benign	Het
Otud3	G	A	4: 138,641,060 (GRCm39)	R27W	probably damaging	Het
Pcare	C	T	17: 72,059,306 (GRCm39)	V124I	possibly damaging	Het
Pcdhga12	T	A	18: 37,901,290 (GRCm39)	F707L	possibly damaging	Het
Pik3r1	T	C	13: 101,894,118 (GRCm39)	E17G	probably damaging	Het
Pramel19	A	G	4: 101,798,788 (GRCm39)	E253G	probably damaging	Het
Prickle4	AAGAGAGAGAGAGAGA	AAGAGAGAGAGAGA	17: 48,000,806 (GRCm39)		probably benign	Het
Prokr1	G	A	6: 87,565,678 (GRCm39)	R56W	probably benign	Het
Ptprz1	G	A	6: 23,024,957 (GRCm39)	C1731Y	probably damaging	Het
Rbm15b	A	T	9: 106,763,028 (GRCm39)	F380Y	possibly damaging	Het
Rfc5	G	A	5: 117,524,885 (GRCm39)	L56F	probably damaging	Het
Rhpn2	A	T	7: 35,090,228 (GRCm39)	M617L	probably benign	Het
Rusc1	T	C	3: 88,995,577 (GRCm39)		probably null	Het
Sec23ip	A	G	7: 128,354,634 (GRCm39)	N297D	probably damaging	Het
Sema3c	A	T	5: 17,880,157 (GRCm39)	H259L	probably benign	Het
Sgsm1	G	A	5: 113,428,328 (GRCm39)		probably benign	Het
Shisal2a	A	G	4: 108,224,998 (GRCm39)	V188A	probably benign	Het
Sipa1l2	T	A	8: 126,218,606 (GRCm39)	S244C	probably damaging	Het
Slc39a5	T	C	10: 128,234,316 (GRCm39)	I196V	probably benign	Het
Smim22	G	A	16: 4,825,722 (GRCm39)	A36T	probably damaging	Het
Spag6	A	G	2: 18,747,588 (GRCm39)	I408V	probably benign	Het
Sult2a5	A	G	7: 13,359,311 (GRCm39)	I96V	probably benign	Het
Tmcc2	C	A	1: 132,308,517 (GRCm39)	A126S	probably benign	Het
Tsc2	A	T	17: 24,819,009 (GRCm39)	S1276T	probably damaging	Het
Ttc21a	A	G	9: 119,788,103 (GRCm39)	S843G	probably benign	Het
Tubd1	G	C	11: 86,443,621 (GRCm39)	V110L	possibly damaging	Het
Ugt1a2	T	A	1: 88,128,534 (GRCm39)	V59D	probably damaging	Het
Vmn2r-ps158	A	G	7: 42,697,024 (GRCm39)	R687G	probably damaging	Het
Vsig8	T	A	1: 172,389,142 (GRCm39)	H131Q	probably benign	Het
Zbtb42	C	A	12: 112,646,861 (GRCm39)	Y345*	probably null	Het
Zfp612	T	A	8: 110,816,576 (GRCm39)	C594*	probably null	Het
Zfp940	A	G	7: 29,544,824 (GRCm39)	V361A	probably benign	Het

Other mutations in Or8c15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Or8c15	APN	9	38,120,534 (GRCm39)	missense	probably damaging	1.00
IGL00914:Or8c15	APN	9	38,121,095 (GRCm39)	splice site	probably null
IGL01563:Or8c15	APN	9	38,120,997 (GRCm39)	missense	probably damaging	1.00
IGL01696:Or8c15	APN	9	38,120,345 (GRCm39)	unclassified	probably benign
IGL02064:Or8c15	APN	9	38,120,874 (GRCm39)	missense	probably damaging	1.00
IGL02605:Or8c15	APN	9	38,120,532 (GRCm39)	missense	probably damaging	0.99
R0415:Or8c15	UTSW	9	38,121,269 (GRCm39)	missense	probably benign
R0463:Or8c15	UTSW	9	38,120,360 (GRCm39)	missense	probably benign	0.00
R0843:Or8c15	UTSW	9	38,120,579 (GRCm39)	missense	possibly damaging	0.81
R1441:Or8c15	UTSW	9	38,120,777 (GRCm39)	missense	probably damaging	1.00
R1939:Or8c15	UTSW	9	38,120,725 (GRCm39)	nonsense	probably null
R2512:Or8c15	UTSW	9	38,120,670 (GRCm39)	missense	probably damaging	1.00
R5439:Or8c15	UTSW	9	38,121,050 (GRCm39)	missense	probably benign	0.00
R6918:Or8c15	UTSW	9	38,120,948 (GRCm39)	missense	possibly damaging	0.95
R7621:Or8c15	UTSW	9	38,120,447 (GRCm39)	missense	probably benign	0.09
R7715:Or8c15	UTSW	9	38,120,775 (GRCm39)	missense	probably benign	0.30
R7940:Or8c15	UTSW	9	38,120,496 (GRCm39)	missense	probably benign	0.00
R8710:Or8c15	UTSW	9	38,121,066 (GRCm39)	missense	possibly damaging	0.60
R8712:Or8c15	UTSW	9	38,121,099 (GRCm39)	missense	probably benign	0.39
R8882:Or8c15	UTSW	9	38,120,461 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACAACCTGAGTTACGGCTGC -3'
(R):5'- CTTGCAAATCCCATTAAGTAGGACC -3'

Sequencing Primer
(F):5'- CCTGAGTTACGGCTGCCTTTG -3'
(R):5'- TTAAGTAGGACCCAATCATTAGCAGG -3'

Posted On

2016-03-17