Mutagenetix > Incidental Mutation

Incidental Mutation 'R4894:Zfp74'

377492

Institutional Source

Beutler Lab

Gene Symbol

Zfp74

Ensembl Gene

ENSMUSG00000059975

Gene Name

zinc finger protein 74

Synonyms

KRAB8, 2810054M15Rik, Zfp66

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R4894 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

29632086-29653579 bp(-) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

T to C at 29635470 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000103847 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032797] [ENSMUST00000108205] [ENSMUST00000108211] [ENSMUST00000108212]

AlphaFold

Q80W31

Predicted Effect

probably benign
Transcript: ENSMUST00000032797

SMART Domains

Protein: ENSMUSP00000032797
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART
ZnF_C2H2	179	201	1.84e-4	SMART
ZnF_C2H2	207	229	2.36e-2	SMART
ZnF_C2H2	235	257	1.5e-4	SMART
ZnF_C2H2	263	285	2.79e-4	SMART
ZnF_C2H2	291	313	3.49e-5	SMART
ZnF_C2H2	319	341	6.42e-4	SMART
ZnF_C2H2	347	369	5.59e-4	SMART
ZnF_C2H2	375	397	5.21e-4	SMART
ZnF_C2H2	403	425	3.63e-3	SMART
ZnF_C2H2	431	453	1.82e-3	SMART
ZnF_C2H2	459	481	1.58e-3	SMART
ZnF_C2H2	487	509	2.12e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.24e-3	SMART
ZnF_C2H2	571	593	8.02e-5	SMART
ZnF_C2H2	599	621	1.04e-3	SMART
ZnF_C2H2	627	649	1.04e-3	SMART
ZnF_C2H2	655	677	9.88e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108205

SMART Domains

Protein: ENSMUSP00000103840
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART
ZnF_C2H2	179	201	1.84e-4	SMART
ZnF_C2H2	207	229	2.36e-2	SMART
ZnF_C2H2	235	257	1.5e-4	SMART
ZnF_C2H2	263	285	2.79e-4	SMART
ZnF_C2H2	291	313	3.49e-5	SMART
ZnF_C2H2	319	341	6.42e-4	SMART
ZnF_C2H2	347	369	5.59e-4	SMART
ZnF_C2H2	375	397	5.21e-4	SMART
ZnF_C2H2	403	425	3.63e-3	SMART
ZnF_C2H2	431	453	1.82e-3	SMART
ZnF_C2H2	459	481	1.58e-3	SMART
ZnF_C2H2	487	509	2.12e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.24e-3	SMART
ZnF_C2H2	571	593	8.02e-5	SMART
ZnF_C2H2	599	621	1.04e-3	SMART
ZnF_C2H2	627	649	1.04e-3	SMART
ZnF_C2H2	655	677	9.88e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108211

SMART Domains

Protein: ENSMUSP00000103846
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108212

SMART Domains

Protein: ENSMUSP00000103847
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149793

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf1	C	A	17: 43,609,975 (GRCm39)	Y176*	probably null	Het
Akap13	T	A	7: 75,375,068 (GRCm39)	M1900K	possibly damaging	Het
Ankrd36	A	G	11: 5,585,332 (GRCm39)	E381G	probably damaging	Het
Ap3s1	T	C	18: 46,891,183 (GRCm39)		probably null	Het
Cacna1e	G	T	1: 154,364,551 (GRCm39)	S341*	probably null	Het
Camk1d	G	A	2: 5,359,539 (GRCm39)	S161L	probably damaging	Het
Cdh23	G	T	10: 60,173,630 (GRCm39)	H1619Q	probably benign	Het
Chd7	T	A	4: 8,838,629 (GRCm39)	I1276N	probably damaging	Het
Clca3a1	A	G	3: 144,719,662 (GRCm39)	V436A	probably damaging	Het
Ctcfl	G	A	2: 172,959,196 (GRCm39)	P177S	probably benign	Het
Dab2ip	A	G	2: 35,620,539 (GRCm39)		probably benign	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Epc1	A	T	18: 6,449,011 (GRCm39)	S495R	probably benign	Het
Espl1	A	G	15: 102,230,758 (GRCm39)		probably null	Het
Eya4	T	C	10: 22,985,753 (GRCm39)	E583G	possibly damaging	Het
Fam111a	C	G	19: 12,565,913 (GRCm39)	T554R	probably benign	Het
Fbh1	A	T	2: 11,767,771 (GRCm39)	I359N	probably damaging	Het
Fer1l6	T	C	15: 58,490,751 (GRCm39)	C1023R	probably damaging	Het
Helz2	G	C	2: 180,877,940 (GRCm39)	P953A	probably benign	Het
Ifi204	G	T	1: 173,587,808 (GRCm39)	S117Y	probably damaging	Het
Ift70a1	T	C	2: 75,810,088 (GRCm39)	*665W	probably null	Het
Igfn1	G	A	1: 135,882,520 (GRCm39)	T2775M	probably damaging	Het
Igsf9	A	G	1: 172,325,634 (GRCm39)	T1101A	probably benign	Het
Ipo13	A	C	4: 117,760,638 (GRCm39)	I614S	probably damaging	Het
Ipo13	A	G	4: 117,761,687 (GRCm39)	I476T	possibly damaging	Het
Kdm2b	C	A	5: 123,079,030 (GRCm39)	E308*	probably null	Het
Klhl20	A	T	1: 160,937,102 (GRCm39)	M91K	possibly damaging	Het
Klrb1f	T	C	6: 129,030,151 (GRCm39)	F64L	probably benign	Het
Ldlrad3	C	T	2: 101,888,293 (GRCm39)	C106Y	probably damaging	Het
Lilra6	T	C	7: 3,915,530 (GRCm39)	T161A	probably benign	Het
Lrriq1	A	T	10: 102,997,613 (GRCm39)	M1334K	possibly damaging	Het
Mepe	C	G	5: 104,473,268 (GRCm39)	P3R	probably damaging	Het
Mgat4e	A	G	1: 134,468,856 (GRCm39)	V396A	probably benign	Het
Nfx1	T	G	4: 40,996,877 (GRCm39)	S651A	probably damaging	Het
Or10ak13	A	T	4: 118,639,483 (GRCm39)	C100S	probably damaging	Het
Or2w6	T	C	13: 21,843,352 (GRCm39)	N47S	probably damaging	Het
Or4c109	T	C	2: 88,817,783 (GRCm39)	I254M	possibly damaging	Het
Rag2	T	C	2: 101,460,022 (GRCm39)	S111P	probably damaging	Het
Rai1	T	A	11: 60,077,572 (GRCm39)	D545E	probably damaging	Het
Ralgps1	A	G	2: 33,033,115 (GRCm39)	V498A	possibly damaging	Het
Rasal2	G	A	1: 157,020,374 (GRCm39)	S205L	probably damaging	Het
Rec8	T	C	14: 55,862,787 (GRCm39)	L582P	probably damaging	Het
Retn	G	A	8: 3,707,358 (GRCm39)	R106H	probably damaging	Het
Rnf112	A	G	11: 61,343,488 (GRCm39)	L116P	probably damaging	Het
Rnf213	T	C	11: 119,372,066 (GRCm39)	Y4885H	probably damaging	Het
Sacm1l	A	G	9: 123,411,409 (GRCm39)	I399M	probably benign	Het
Sez6	G	T	11: 77,866,086 (GRCm39)	G738V	probably damaging	Het
Spata17	A	G	1: 186,872,643 (GRCm39)	V56A	probably benign	Het
Spata31d1a	A	T	13: 59,849,542 (GRCm39)	V862D	probably damaging	Het
Sptb	A	G	12: 76,671,768 (GRCm39)		probably null	Het
Srpk2	C	A	5: 23,750,527 (GRCm39)	G59W	probably damaging	Het
Tyro3	T	C	2: 119,632,779 (GRCm39)	S96P	probably damaging	Het
Ube2v1	A	G	2: 167,452,280 (GRCm39)	S108P	probably damaging	Het
Usp2	C	T	9: 43,987,125 (GRCm39)	S141L	probably benign	Het
Vamp5	T	C	6: 72,347,181 (GRCm39)	D46G	possibly damaging	Het
Vmn1r23	T	A	6: 57,903,310 (GRCm39)	Q156L	probably benign	Het
Vmn2r6	C	T	3: 64,454,829 (GRCm39)	S490N	probably benign	Het
Vps39	A	T	2: 120,183,440 (GRCm39)	I10N	probably damaging	Het
Vwf	C	T	6: 125,622,897 (GRCm39)	Q1755*	probably null	Het
Wdfy4	A	T	14: 32,877,717 (GRCm39)	H82Q	probably benign	Het
Wdr24	T	C	17: 26,045,101 (GRCm39)	Y279H	probably damaging	Het
Wdr72	A	G	9: 74,117,843 (GRCm39)	T852A	probably benign	Het
Zfp1	T	A	8: 112,396,355 (GRCm39)	C92*	probably null	Het
Zfp1004	T	A	2: 150,033,899 (GRCm39)	C104*	probably null	Het
Zfp426	A	T	9: 20,386,369 (GRCm39)		probably benign	Het
Zfp442	C	T	2: 150,253,130 (GRCm39)		probably null	Het

Other mutations in Zfp74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0355:Zfp74	UTSW	7	29,653,466 (GRCm39)	start gained	probably benign
R0387:Zfp74	UTSW	7	29,634,179 (GRCm39)	missense	probably benign	0.05
R0948:Zfp74	UTSW	7	29,635,362 (GRCm39)	critical splice donor site	probably null
R1757:Zfp74	UTSW	7	29,634,486 (GRCm39)	missense	probably benign	0.01
R1813:Zfp74	UTSW	7	29,634,569 (GRCm39)	missense	probably damaging	1.00
R1893:Zfp74	UTSW	7	29,635,470 (GRCm39)	critical splice acceptor site	probably null
R1896:Zfp74	UTSW	7	29,634,569 (GRCm39)	missense	probably damaging	1.00
R1958:Zfp74	UTSW	7	29,635,136 (GRCm39)	missense	probably benign	0.08
R2092:Zfp74	UTSW	7	29,653,349 (GRCm39)	start gained	probably benign
R2111:Zfp74	UTSW	7	29,634,443 (GRCm39)	nonsense	probably null
R5121:Zfp74	UTSW	7	29,631,932 (GRCm39)	splice site	probably null
R5123:Zfp74	UTSW	7	29,634,158 (GRCm39)	missense	probably damaging	1.00
R5129:Zfp74	UTSW	7	29,631,880 (GRCm39)	missense	probably benign	0.00
R5213:Zfp74	UTSW	7	29,634,668 (GRCm39)	missense	probably damaging	1.00
R5460:Zfp74	UTSW	7	29,635,316 (GRCm39)	missense	probably benign	0.04
R5519:Zfp74	UTSW	7	29,634,559 (GRCm39)	missense	probably damaging	0.99
R5589:Zfp74	UTSW	7	29,633,990 (GRCm39)	missense	probably damaging	1.00
R6287:Zfp74	UTSW	7	29,635,201 (GRCm39)	missense	probably benign
R6330:Zfp74	UTSW	7	29,637,412 (GRCm39)	missense	probably damaging	1.00
R6370:Zfp74	UTSW	7	29,631,835 (GRCm39)	missense	probably damaging	0.96
R6407:Zfp74	UTSW	7	29,635,048 (GRCm39)	missense	probably damaging	1.00
R6694:Zfp74	UTSW	7	29,634,559 (GRCm39)	missense	probably damaging	0.99
R6791:Zfp74	UTSW	7	29,633,860 (GRCm39)	missense	probably benign	0.02
R7144:Zfp74	UTSW	7	29,634,590 (GRCm39)	missense	probably damaging	0.98
R7662:Zfp74	UTSW	7	29,653,278 (GRCm39)	critical splice donor site	probably null
R7667:Zfp74	UTSW	7	29,634,608 (GRCm39)	missense	probably damaging	1.00
R7898:Zfp74	UTSW	7	29,635,380 (GRCm39)	nonsense	probably null
R7940:Zfp74	UTSW	7	29,631,867 (GRCm39)	missense	probably benign	0.07
R8676:Zfp74	UTSW	7	29,634,079 (GRCm39)	missense	probably damaging	1.00
R8864:Zfp74	UTSW	7	29,634,235 (GRCm39)	missense	probably damaging	1.00
R8940:Zfp74	UTSW	7	29,634,772 (GRCm39)	missense	possibly damaging	0.96
R9748:Zfp74	UTSW	7	29,634,751 (GRCm39)	missense	probably damaging	1.00
R9764:Zfp74	UTSW	7	29,631,845 (GRCm39)	missense	probably benign	0.11

Predicted Primers

PCR Primer
(F):5'- ACCGTCACATTCAAAGAGACTG -3'
(R):5'- GGAACTTCCTTCTAAAACTGAGAAGC -3'

Sequencing Primer
(F):5'- CACATTCAAAGAGACTGTGCATG -3'
(R):5'- GAGTACACTGTAGCTGTCTTCAGAC -3'

Posted On

2016-03-17