Mutagenetix > Incidental Mutation

Incidental Mutation 'R4908:Scn9a'

379158

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

042510-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4908 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66310424-66465306 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 66357087 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 1062 (D1062E)

Ref Sequence

ENSEMBL: ENSMUSP00000097641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

probably benign
Transcript: ENSMUST00000100063
AA Change: D1062E

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: D1062E

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100064
AA Change: D1071E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: D1071E

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112354
AA Change: D1060E

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: D1060E

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000119700

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141149

Predicted Effect

probably benign
Transcript: ENSMUST00000164384
AA Change: D1071E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: D1071E

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000169900
AA Change: D1060E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: D1060E

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059G10Rik	T	A	9: 122,778,008 (GRCm39)	I79L	probably benign	Het
Ablim2	C	T	5: 35,959,766 (GRCm39)	R73C	possibly damaging	Het
Acaa1a	G	A	9: 119,177,772 (GRCm39)	S218N	probably benign	Het
Acsm5	A	T	7: 119,137,314 (GRCm39)	I377F	probably damaging	Het
Ahnak2	T	C	12: 112,741,706 (GRCm39)	T789A	probably benign	Het
Ak9	A	T	10: 41,296,678 (GRCm39)	T1475S	unknown	Het
AU040320	A	T	4: 126,747,081 (GRCm39)	N1028Y	probably damaging	Het
Bahcc1	T	A	11: 120,178,580 (GRCm39)	S2380T	probably benign	Het
Cadps	A	G	14: 12,536,386 (GRCm38)	Y525H	probably damaging	Het
Casp8ap2	A	G	4: 32,639,905 (GRCm39)	T320A	possibly damaging	Het
Ccdc138	A	G	10: 58,380,817 (GRCm39)	N483D	possibly damaging	Het
Ccdc39	G	A	3: 33,893,242 (GRCm39)		probably null	Het
Cd300c2	T	C	11: 114,887,772 (GRCm39)	N210S	probably damaging	Het
Cd84	A	G	1: 171,700,432 (GRCm39)	D183G	probably damaging	Het
Cep95	C	T	11: 106,702,172 (GRCm39)	P390S	probably damaging	Het
Chd9	A	T	8: 91,741,877 (GRCm39)	H1622L	possibly damaging	Het
Cilp	C	A	9: 65,185,302 (GRCm39)	Q466K	probably benign	Het
Cinp	T	A	12: 110,850,487 (GRCm39)	T5S	probably damaging	Het
Clec4a2	T	C	6: 123,119,462 (GRCm39)	L238S	probably damaging	Het
Cntrob	T	C	11: 69,211,732 (GRCm39)	Y164C	probably damaging	Het
Col6a3	A	G	1: 90,735,246 (GRCm39)	L1408P	probably damaging	Het
Cul3	G	A	1: 80,258,632 (GRCm39)	S468L	possibly damaging	Het
Dnah11	T	A	12: 118,090,618 (GRCm39)	D1081V	probably benign	Het
Dnah2	C	A	11: 69,411,973 (GRCm39)	V263L	probably benign	Het
Efna3	C	G	3: 89,222,805 (GRCm39)	R185P	probably damaging	Het
F5	A	T	1: 164,039,389 (GRCm39)	I2000F	probably damaging	Het
Fancm	G	A	12: 65,141,645 (GRCm39)	G422E	probably benign	Het
Gcnt2	A	T	13: 41,014,210 (GRCm39)	D127V	probably damaging	Het
Gm21798	G	T	15: 64,689,618 (GRCm39)		probably benign	Het
Gramd1a	A	C	7: 30,838,292 (GRCm39)	S320R	probably benign	Het
Grn	T	C	11: 102,327,344 (GRCm39)		probably benign	Het
Helq	A	T	5: 100,910,507 (GRCm39)		probably null	Het
Herc2	A	G	7: 55,827,660 (GRCm39)	I2914V	probably benign	Het
Hnrnph1	T	C	11: 50,269,237 (GRCm39)	V27A	probably damaging	Het
Hs1bp3	G	T	12: 8,374,007 (GRCm39)	G182C	probably damaging	Het
Idnk	C	T	13: 58,311,267 (GRCm39)	P78L	probably benign	Het
Il10ra	T	C	9: 45,166,919 (GRCm39)	D544G	probably benign	Het
Inpp5e	T	C	2: 26,290,918 (GRCm39)	D383G	probably damaging	Het
Jak1	A	T	4: 101,036,911 (GRCm39)	V243D	probably damaging	Het
Kcnma1	A	T	14: 23,359,220 (GRCm39)	S1036T	probably damaging	Het
Kif26a	G	A	12: 112,123,776 (GRCm39)	C127Y	probably damaging	Het
Kif2c	T	C	4: 117,023,608 (GRCm39)	E368G	probably damaging	Het
Lasp1	T	A	11: 97,724,530 (GRCm39)		probably null	Het
Lcor	T	A	19: 41,572,601 (GRCm39)	V452D	probably benign	Het
Lrrtm1	C	G	6: 77,221,661 (GRCm39)	H373D	probably benign	Het
Matr3	T	A	18: 35,705,754 (GRCm39)	D226E	probably damaging	Het
Mmp17	G	A	5: 129,682,730 (GRCm39)	W456*	probably null	Het
Mpp4	T	C	1: 59,164,748 (GRCm39)	E463G	probably damaging	Het
Myh6	A	C	14: 55,194,419 (GRCm39)	F737V	probably damaging	Het
Nars2	T	A	7: 96,672,948 (GRCm39)	D271E	probably benign	Het
Nav2	A	G	7: 49,254,258 (GRCm39)	E2352G	probably damaging	Het
Nckap1	A	G	2: 80,353,718 (GRCm39)		probably null	Het
Nckap5	A	T	1: 125,955,324 (GRCm39)	S477R	probably damaging	Het
Nek11	T	G	9: 105,175,488 (GRCm39)	I319L	probably benign	Het
Neto2	A	G	8: 86,396,393 (GRCm39)	I84T	probably damaging	Het
Nlrp9a	A	T	7: 26,250,369 (GRCm39)	I45F	probably damaging	Het
Numa1	G	A	7: 101,662,012 (GRCm39)	R548H	probably damaging	Het
Or10a5	A	C	7: 106,635,364 (GRCm39)	M1L	probably benign	Het
Or14c39	A	C	7: 86,344,395 (GRCm39)	I244L	probably benign	Het
Or2ag1b	A	T	7: 106,288,740 (GRCm39)	L66H	probably damaging	Het
Or4c10	A	T	2: 89,760,923 (GRCm39)	M257L	probably benign	Het
Or4c100	T	A	2: 88,356,254 (GRCm39)	I109N	probably damaging	Het
Or4k36	T	A	2: 111,146,574 (GRCm39)	F250Y	probably benign	Het
Or7g25	A	G	9: 19,160,149 (GRCm39)	V182A	probably benign	Het
Or9m1	A	T	2: 87,733,533 (GRCm39)	N162K	probably damaging	Het
Pcdhb16	T	A	18: 37,612,894 (GRCm39)		probably null	Het
Pdcd10	T	C	3: 75,448,553 (GRCm39)	T4A	probably damaging	Het
Pgc	A	T	17: 48,039,819 (GRCm39)	Y71F	probably damaging	Het
Phlpp1	G	A	1: 106,317,481 (GRCm39)	G1234E	probably damaging	Het
Prr27	T	C	5: 87,990,888 (GRCm39)	F167L	probably benign	Het
Prrc2b	T	A	2: 32,116,330 (GRCm39)	S1421T	possibly damaging	Het
Pxmp2	A	G	5: 110,431,518 (GRCm39)	V75A	probably benign	Het
Pygl	A	T	12: 70,243,807 (GRCm39)	M545K	probably null	Het
Ranbp9	A	G	13: 43,574,733 (GRCm39)	Y412H	possibly damaging	Het
Rcc1	A	G	4: 132,065,064 (GRCm39)	V140A	probably damaging	Het
Reln	A	G	5: 22,184,718 (GRCm39)	V1599A	probably benign	Het
Rhebl1	A	T	15: 98,776,903 (GRCm39)	D122E	probably damaging	Het
Rock2	C	T	12: 17,009,492 (GRCm39)	L676F	probably benign	Het
Sec63	T	C	10: 42,681,186 (GRCm39)	I390T	probably damaging	Het
Slc10a6	T	C	5: 103,754,493 (GRCm39)	E346G	probably benign	Het
Slc12a3	G	A	8: 95,075,216 (GRCm39)	V737M	possibly damaging	Het
Slc12a8	T	C	16: 33,426,629 (GRCm39)		probably null	Het
Slc25a38	T	A	9: 119,949,354 (GRCm39)	I102N	probably damaging	Het
Spg7	T	A	8: 123,807,394 (GRCm39)	V390E	probably damaging	Het
Tbc1d20	T	C	2: 152,144,228 (GRCm39)	V75A	probably benign	Het
Tesk1	T	A	4: 43,445,555 (GRCm39)	C243*	probably null	Het
Ttk	T	A	9: 83,725,739 (GRCm39)	N220K	possibly damaging	Het
Ttll5	A	G	12: 85,965,948 (GRCm39)	E651G	probably benign	Het
Tubd1	T	C	11: 86,457,879 (GRCm39)	Y426H	probably damaging	Het
Uba6	T	C	5: 86,288,293 (GRCm39)		silent	Het
Ube2ql1	G	A	13: 69,852,289 (GRCm39)	R263W	probably damaging	Het
Vmn2r117	C	T	17: 23,678,812 (GRCm39)	G804D	probably damaging	Het
Vmn2r25	C	T	6: 123,805,406 (GRCm39)	E484K	probably benign	Het
Vmn2r4	T	A	3: 64,296,476 (GRCm39)	I770F	possibly damaging	Het
Vmn2r82	G	A	10: 79,214,589 (GRCm39)	V191M	probably benign	Het
Zbtb45	C	T	7: 12,742,037 (GRCm39)	V74M	probably damaging	Het
Zfp366	T	C	13: 99,370,609 (GRCm39)	V443A	possibly damaging	Het
Zfyve1	A	T	12: 83,598,345 (GRCm39)	C628S	probably damaging	Het
Zfyve26	A	T	12: 79,296,469 (GRCm39)		probably null	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,393,945 (GRCm39)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,314,486 (GRCm39)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,314,279 (GRCm39)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,314,645 (GRCm39)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,357,316 (GRCm39)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,318,397 (GRCm39)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,357,416 (GRCm39)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,367,722 (GRCm39)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,324,230 (GRCm39)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,317,986 (GRCm39)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,314,386 (GRCm39)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,314,777 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,325,170 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,377,479 (GRCm39)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,323,447 (GRCm39)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,314,955 (GRCm39)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,366,440 (GRCm39)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,367,637 (GRCm39)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,370,903 (GRCm39)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,392,855 (GRCm39)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,314,358 (GRCm39)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,357,091 (GRCm39)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,392,788 (GRCm39)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,363,437 (GRCm39)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,398,475 (GRCm39)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,335,354 (GRCm39)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,373,621 (GRCm39)	missense	probably benign
R0465:Scn9a	UTSW	2	66,357,340 (GRCm39)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,314,022 (GRCm39)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,357,143 (GRCm39)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,367,721 (GRCm39)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,363,405 (GRCm39)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,363,721 (GRCm39)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,313,843 (GRCm39)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,377,456 (GRCm39)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,366,468 (GRCm39)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,314,079 (GRCm39)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,335,341 (GRCm39)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,393,886 (GRCm39)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,314,930 (GRCm39)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,357,232 (GRCm39)	missense	probably benign
R1511:Scn9a	UTSW	2	66,357,157 (GRCm39)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,335,371 (GRCm39)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,314,648 (GRCm39)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,318,361 (GRCm39)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,313,803 (GRCm39)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,335,220 (GRCm39)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,313,850 (GRCm39)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,317,938 (GRCm39)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,332,060 (GRCm39)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,396,594 (GRCm39)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,314,655 (GRCm39)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,345,724 (GRCm39)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,345,665 (GRCm39)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,363,720 (GRCm39)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,398,527 (GRCm39)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,356,998 (GRCm39)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,366,669 (GRCm39)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,364,573 (GRCm39)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,392,749 (GRCm39)	missense	probably benign
R3771:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,395,398 (GRCm39)	intron	probably benign
R4385:Scn9a	UTSW	2	66,314,900 (GRCm39)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,357,037 (GRCm39)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,313,902 (GRCm39)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,377,362 (GRCm39)	missense	probably benign
R4783:Scn9a	UTSW	2	66,370,967 (GRCm39)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,314,093 (GRCm39)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,382,057 (GRCm39)	missense	probably benign
R4983:Scn9a	UTSW	2	66,396,614 (GRCm39)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,392,824 (GRCm39)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,364,463 (GRCm39)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,395,511 (GRCm39)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,318,387 (GRCm39)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,377,447 (GRCm39)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,395,373 (GRCm39)	intron	probably benign
R6108:Scn9a	UTSW	2	66,314,393 (GRCm39)	missense	probably damaging	1.00
R6115:Scn9a	UTSW	2	66,393,973 (GRCm39)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,317,868 (GRCm39)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,314,240 (GRCm39)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,398,608 (GRCm39)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,357,307 (GRCm39)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,313,846 (GRCm39)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,393,686 (GRCm39)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,379,359 (GRCm39)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,364,567 (GRCm39)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,314,748 (GRCm39)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,314,931 (GRCm39)	missense	probably benign
R7386:Scn9a	UTSW	2	66,370,894 (GRCm39)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,377,531 (GRCm39)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,363,692 (GRCm39)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,364,561 (GRCm39)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,313,990 (GRCm39)	missense	probably benign
R7617:Scn9a	UTSW	2	66,370,893 (GRCm39)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,366,580 (GRCm39)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,357,424 (GRCm39)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,314,642 (GRCm39)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,314,135 (GRCm39)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,314,904 (GRCm39)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,373,456 (GRCm39)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,314,597 (GRCm39)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,345,774 (GRCm39)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,317,754 (GRCm39)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,314,745 (GRCm39)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,366,626 (GRCm39)	missense	probably benign
R8345:Scn9a	UTSW	2	66,324,966 (GRCm39)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,396,625 (GRCm39)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,332,015 (GRCm39)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,366,628 (GRCm39)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,332,010 (GRCm39)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,370,979 (GRCm39)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,313,961 (GRCm39)	missense	probably benign
R9009:Scn9a	UTSW	2	66,338,927 (GRCm39)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,325,147 (GRCm39)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,314,744 (GRCm39)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,363,657 (GRCm39)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,335,236 (GRCm39)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,314,261 (GRCm39)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,357,040 (GRCm39)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,395,553 (GRCm39)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,314,328 (GRCm39)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,363,708 (GRCm39)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,392,809 (GRCm39)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,357,002 (GRCm39)	missense	probably benign
X0003:Scn9a	UTSW	2	66,338,991 (GRCm39)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,398,421 (GRCm39)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,370,936 (GRCm39)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,325,029 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- TGGGCAACATTAGACCTGAG -3'
(R):5'- GACCCTGCGTGAATTCATTC -3'

Sequencing Primer
(F):5'- TGGGCAACATTAGACCTGAGATATC -3'
(R):5'- GACCCTGCGTGAATTCATTCTAAAG -3'

Posted On

2016-04-15