Mutagenetix > Incidental Mutation

Incidental Mutation 'R4934:Sspo'

380795

Institutional Source

Beutler Lab

Gene Symbol

Sspo

Ensembl Gene

ENSMUSG00000029797

Gene Name

SCO-spondin

Synonyms

C79529, Scospondin

MMRRC Submission

042534-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4934 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

48425163-48478184 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 48442486 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Isoleucine at position 1994 (L1994I)

Ref Sequence

ENSEMBL: ENSMUSP00000131401 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043676] [ENSMUST00000169350] [ENSMUST00000212740]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000043676
AA Change: L1853I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000047991
Gene: ENSMUSG00000029797
AA Change: L1853I

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	126	135	N/A	INTRINSIC
Pfam:VWD	154	219	7.4e-11	PFAM
C8	267	346	2.3e-10	SMART
Pfam:TIL	349	404	3.2e-13	PFAM
VWC	406	448	2e-1	SMART
VWD	433	593	5.08e-29	SMART
C8	631	703	2.14e-28	SMART
Pfam:TIL	706	759	5.8e-11	PFAM
VWC	856	924	4.76e-2	SMART
VWD	883	1042	9.59e-48	SMART
C8	1076	1150	3.62e-26	SMART
Pfam:TIL	1153	1209	2.6e-13	PFAM
LDLa	1253	1291	2.29e-13	SMART
LDLa	1293	1328	1.87e-9	SMART
LDLa	1329	1366	5.77e-10	SMART
LDLa	1369	1408	1.52e-9	SMART
LDLa	1442	1479	2.55e-11	SMART
LDLa	1480	1520	5.6e-8	SMART
LDLa	1533	1574	2.29e-4	SMART
TSP1	1575	1626	6.47e-13	SMART
TSP1	1631	1686	1.35e-10	SMART
Pfam:TIL	1690	1746	3.1e-9	PFAM
TSP1	1774	1827	6.94e-2	SMART
VWC	1829	1886	4.95e-9	SMART
low complexity region	1901	1911	N/A	INTRINSIC
FA58C	1928	2085	1.4e-2	SMART
LDLa	2091	2128	1.48e-7	SMART
LDLa	2242	2279	5.68e-9	SMART
LDLa	2299	2336	5.77e-10	SMART
TSP1	2339	2389	1.42e-9	SMART
TSP1	2394	2446	6.36e-21	SMART
Pfam:TIL	2460	2511	5.7e-10	PFAM
VWC	2513	2567	2.48e-1	SMART
TSP1	2554	2605	3.07e-14	SMART
TSP1	2611	2664	4.05e-5	SMART
TSP1	2669	2719	1.83e-12	SMART
EGF_like	2733	2776	5.45e1	SMART
VWC	2783	2836	2.73e-11	SMART
TSP1	2823	2875	3.72e-13	SMART
TSP1	2878	2919	6.05e-4	SMART
Pfam:TIL	2926	2978	1.1e-11	PFAM
VWC	2980	3035	9.77e-2	SMART
TSP1	3022	3086	6.68e-6	SMART
TSP1	3091	3143	1.08e-14	SMART
Pfam:TIL	3147	3201	2.2e-9	PFAM
VWC	3203	3260	2.72e-1	SMART
TSP1	3247	3306	3.72e-4	SMART
TSP1	3311	3363	5.27e-4	SMART
Pfam:TIL	3365	3421	4.2e-9	PFAM
TSP1	3484	3529	1.87e-9	SMART
low complexity region	3591	3601	N/A	INTRINSIC
TSP1	3660	3713	5.02e-10	SMART
TSP1	3730	3779	2.95e-7	SMART
TSP1	3796	3849	1.99e-13	SMART
TSP1	3854	3906	2.51e-10	SMART
Pfam:TIL	3909	3964	3.4e-11	PFAM
VWC	3966	4022	1.26e0	SMART
TSP1	4009	4059	4.05e-5	SMART
TSP1	4103	4155	3.19e-12	SMART
TSP1	4161	4213	2.87e-2	SMART
TSP1	4218	4269	1.45e-6	SMART
Pfam:TIL	4273	4328	2.1e-10	PFAM
TSP1	4468	4516	7.56e-5	SMART
low complexity region	4551	4562	N/A	INTRINSIC
VWC	4578	4652	5.21e-1	SMART
TSP1	4619	4669	3.92e-12	SMART
Pfam:TIL	4671	4725	1.5e-11	PFAM
Pfam:TIL	4777	4835	3.1e-9	PFAM
VWC	4837	4892	1.8e-11	SMART
GHB	4904	4997	1.02e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000169350
AA Change: L1994I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131401
Gene: ENSMUSG00000029797
AA Change: L1994I

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	126	135	N/A	INTRINSIC
VWD	185	341	4.36e-28	SMART
C8	390	469	2.3e-10	SMART
Pfam:TIL	472	527	8.6e-13	PFAM
VWC	529	571	2e-1	SMART
VWD	556	716	5.08e-29	SMART
C8	754	826	2.14e-28	SMART
Pfam:TIL	829	882	1.6e-10	PFAM
VWC	979	1047	4.76e-2	SMART
VWD	1006	1165	9.59e-48	SMART
C8	1201	1275	3.62e-26	SMART
Pfam:TIL	1278	1334	7e-13	PFAM
LDLa	1378	1416	2.29e-13	SMART
LDLa	1418	1453	1.87e-9	SMART
LDLa	1454	1491	5.77e-10	SMART
LDLa	1494	1533	1.52e-9	SMART
LDLa	1567	1604	2.55e-11	SMART
LDLa	1605	1645	5.6e-8	SMART
LDLa	1658	1699	2.29e-4	SMART
TSP1	1700	1751	6.47e-13	SMART
TSP1	1756	1811	1.35e-10	SMART
Pfam:TIL	1815	1871	8.3e-9	PFAM
VWC	1873	1928	2.42e-1	SMART
TSP1	1915	1968	6.94e-2	SMART
VWC	1970	2027	4.95e-9	SMART
low complexity region	2042	2052	N/A	INTRINSIC
FA58C	2069	2226	1.4e-2	SMART
LDLa	2232	2269	1.48e-7	SMART
LDLa	2387	2424	5.68e-9	SMART
LDLa	2444	2481	5.77e-10	SMART
TSP1	2484	2534	1.42e-9	SMART
TSP1	2539	2591	6.36e-21	SMART
Pfam:TIL	2606	2656	1.8e-9	PFAM
VWC	2658	2712	2.48e-1	SMART
TSP1	2699	2750	3.07e-14	SMART
TSP1	2756	2809	4.05e-5	SMART
TSP1	2814	2864	1.83e-12	SMART
EGF_like	2878	2921	5.45e1	SMART
VWC	2928	2981	2.73e-11	SMART
TSP1	2968	3020	3.72e-13	SMART
TSP1	3023	3064	6.05e-4	SMART
Pfam:TIL	3071	3123	3e-11	PFAM
VWC	3125	3180	9.77e-2	SMART
TSP1	3167	3231	6.68e-6	SMART
TSP1	3236	3288	1.08e-14	SMART
Pfam:TIL	3292	3346	6e-9	PFAM
VWC	3348	3405	2.72e-1	SMART
TSP1	3392	3451	3.72e-4	SMART
TSP1	3456	3508	5.27e-4	SMART
Pfam:TIL	3510	3566	1.1e-8	PFAM
TSP1	3629	3674	1.87e-9	SMART
low complexity region	3734	3744	N/A	INTRINSIC
TSP1	3803	3856	5.02e-10	SMART
TSP1	3873	3922	2.95e-7	SMART
TSP1	3939	3992	1.99e-13	SMART
TSP1	3997	4049	2.51e-10	SMART
Pfam:TIL	4052	4107	9.1e-11	PFAM
VWC	4109	4165	1.26e0	SMART
TSP1	4152	4202	4.05e-5	SMART
TSP1	4246	4298	3.19e-12	SMART
TSP1	4304	4356	2.87e-2	SMART
TSP1	4361	4412	1.45e-6	SMART
Pfam:TIL	4416	4471	5.6e-10	PFAM
TSP1	4611	4659	7.56e-5	SMART
low complexity region	4694	4705	N/A	INTRINSIC
VWC	4721	4795	5.21e-1	SMART
TSP1	4762	4812	3.92e-12	SMART
Pfam:TIL	4814	4868	4e-11	PFAM
Pfam:TIL	4920	4978	8.4e-9	PFAM
VWC	4980	5035	1.8e-11	SMART
GHB	5050	5143	1.02e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000212740
AA Change: L1994I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.0%
20x: 91.4%

Validation Efficiency

99% (134/135)

Allele List at MGI

Other mutations in this stock

Total: 125 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931428L18Rik	A	T	1: 31,261,580 (GRCm39)		probably benign	Het
Acy1	T	A	9: 106,312,321 (GRCm39)	I14F	probably null	Het
Ago1	T	C	4: 126,342,652 (GRCm39)	D193G	possibly damaging	Het
Arhgap45	A	T	10: 79,856,791 (GRCm39)	H201L	probably damaging	Het
Armc9	G	A	1: 86,140,801 (GRCm39)	D63N	probably damaging	Het
Asb17	A	T	3: 153,556,336 (GRCm39)	I148F	possibly damaging	Het
Atf7ip2	A	G	16: 10,059,447 (GRCm39)	E329G	possibly damaging	Het
Atp2a1	T	A	7: 126,052,600 (GRCm39)	D373V	probably benign	Het
Cacna1e	G	T	1: 154,357,380 (GRCm39)	Y603*	probably null	Het
Cbln4	G	A	2: 171,880,901 (GRCm39)	T131I	probably damaging	Het
Ccdc126	A	G	6: 49,311,181 (GRCm39)	E63G	probably damaging	Het
Ccl4	T	A	11: 83,553,504 (GRCm39)	S6T	unknown	Het
Ccnb1	A	T	13: 100,918,209 (GRCm39)	I146K	possibly damaging	Het
Ccr8	T	A	9: 119,923,815 (GRCm39)	M310K	probably benign	Het
Cd180	A	T	13: 102,875,672 (GRCm39)		probably null	Het
Cd46	G	C	1: 194,765,107 (GRCm39)		probably benign	Het
Cebpb	A	G	2: 167,531,005 (GRCm39)	M22V	probably benign	Het
Cep152	A	G	2: 125,453,016 (GRCm39)	I352T	possibly damaging	Het
Cep295	T	G	9: 15,244,456 (GRCm39)	E1333D	probably damaging	Het
Ces4a	A	G	8: 105,864,613 (GRCm39)	H30R	probably benign	Het
Chrm3	A	G	13: 9,927,450 (GRCm39)	Y529H	probably damaging	Het
Chrnb4	T	C	9: 54,942,101 (GRCm39)	Y391C	probably benign	Het
Clca3a2	A	G	3: 144,523,692 (GRCm39)	Y98H	probably damaging	Het
Clvs1	C	T	4: 9,424,216 (GRCm39)	H221Y	possibly damaging	Het
Col3a1	G	A	1: 45,379,112 (GRCm39)		probably benign	Het
Cpn2	T	G	16: 30,079,344 (GRCm39)	N119T	probably damaging	Het
Cspg4b	C	A	13: 113,504,882 (GRCm39)	Q2004K	probably damaging	Het
Cubn	T	A	2: 13,494,721 (GRCm39)	Q109H	probably benign	Het
Cyp26b1	A	G	6: 84,553,954 (GRCm39)	V221A	possibly damaging	Het
Cyp2e1	T	G	7: 140,350,030 (GRCm39)	N238K	probably damaging	Het
Dst	C	T	1: 34,247,669 (GRCm39)	A1693V	probably damaging	Het
Dync2li1	A	G	17: 84,956,683 (GRCm39)	Q281R	probably benign	Het
Enpp2	C	T	15: 54,745,543 (GRCm39)	G318S	probably damaging	Het
Entrep1	T	C	19: 23,950,789 (GRCm39)	*597W	probably null	Het
F13a1	G	T	13: 37,061,736 (GRCm39)	P676T	probably benign	Het
Fam227a	T	C	15: 79,521,262 (GRCm39)	H267R	possibly damaging	Het
Fam78a	G	A	2: 31,959,427 (GRCm39)	R228C	probably damaging	Het
Fbxw25	T	C	9: 109,480,705 (GRCm39)	N325S	possibly damaging	Het
Foxred1	T	C	9: 35,121,210 (GRCm39)		probably benign	Het
Fstl5	T	A	3: 76,496,272 (GRCm39)	V345E	probably damaging	Het
Fzd8	GAAAAACTCA	GA	18: 9,214,492 (GRCm39)		probably null	Het
Hand1	T	C	11: 57,722,078 (GRCm39)	R179G	possibly damaging	Het
Hk1	G	T	10: 62,194,165 (GRCm39)		probably benign	Het
Hmcn1	A	C	1: 150,598,286 (GRCm39)	L1672R	probably damaging	Het
Hps5	G	A	7: 46,418,775 (GRCm39)	Q297*	probably null	Het
Iars1	T	A	13: 49,871,460 (GRCm39)	F699I	probably benign	Het
Ift140	G	A	17: 25,267,462 (GRCm39)	G620E	probably benign	Het
Igkv4-80	A	C	6: 68,993,649 (GRCm39)	S81A	probably benign	Het
Inpp4a	T	G	1: 37,426,922 (GRCm39)	Y628D	possibly damaging	Het
Itk	C	T	11: 46,280,152 (GRCm39)	R29H	probably damaging	Het
Kcnv1	A	G	15: 44,972,644 (GRCm39)	F413S	probably damaging	Het
Klhl3	A	T	13: 58,250,231 (GRCm39)	Y4*	probably null	Het
Lonrf1	C	A	8: 36,701,103 (GRCm39)	C369F	probably damaging	Het
Magohb	A	T	6: 131,261,558 (GRCm39)		probably benign	Het
Map3k4	G	A	17: 12,490,787 (GRCm39)	R215C	probably damaging	Het
Map3k8	A	T	18: 4,339,548 (GRCm39)	S274R	possibly damaging	Het
Masp1	T	G	16: 23,283,826 (GRCm39)	M470L	probably damaging	Het
Mfng	C	T	15: 78,648,588 (GRCm39)	R163H	probably benign	Het
Muc4	A	T	16: 32,576,472 (GRCm39)		probably benign	Het
Myo1c	T	A	11: 75,562,676 (GRCm39)	V981E	probably damaging	Het
Nedd9	A	T	13: 41,492,411 (GRCm39)	I27K	probably damaging	Het
Nkd2	C	A	13: 73,970,841 (GRCm39)	G247V	probably damaging	Het
Nucb2	A	C	7: 116,139,199 (GRCm39)	Q398P	possibly damaging	Het
Numa1	T	A	7: 101,660,064 (GRCm39)	D376E	probably benign	Het
Or2w1	G	A	13: 21,317,241 (GRCm39)	V99I	probably benign	Het
Or4c101	C	T	2: 88,389,930 (GRCm39)	T39I	probably benign	Het
Or4n5	T	C	14: 50,133,206 (GRCm39)	T18A	probably benign	Het
Or4p23	A	T	2: 88,576,398 (GRCm39)	L278*	probably null	Het
Or4q3	C	T	14: 50,583,345 (GRCm39)	V185M	probably damaging	Het
Or5b119	G	A	19: 13,456,956 (GRCm39)	T202I	possibly damaging	Het
Or6c76b	A	T	10: 129,692,896 (GRCm39)	N170Y	possibly damaging	Het
Or8d23	A	T	9: 38,842,129 (GRCm39)	I221F	probably damaging	Het
Otx1	C	A	11: 21,947,037 (GRCm39)	A91S	probably damaging	Het
Pamr1	C	T	2: 102,472,549 (GRCm39)	T616I	probably benign	Het
Pcnx1	G	A	12: 82,038,599 (GRCm39)	V1955I	possibly damaging	Het
Pira13	A	G	7: 3,825,676 (GRCm39)	Y398H	probably damaging	Het
Plekha3	T	A	2: 76,510,571 (GRCm39)	D35E	possibly damaging	Het
Pnpla2	T	G	7: 141,038,085 (GRCm39)	N184K	probably damaging	Het
Polr3a	C	T	14: 24,502,692 (GRCm39)	E1216K	probably benign	Het
Ppp1r21	A	T	17: 88,852,803 (GRCm39)	S61C	probably damaging	Het
Ppp1r21	G	C	17: 88,852,804 (GRCm39)	S61T	probably damaging	Het
Prdm13	G	T	4: 21,678,223 (GRCm39)		probably benign	Het
Prss53	T	C	7: 127,487,879 (GRCm39)	N201S	probably benign	Het
Rab11fip2	A	G	19: 59,924,290 (GRCm39)	L338S	probably damaging	Het
Rad50	T	C	11: 53,575,102 (GRCm39)	N546S	probably benign	Het
Ralgapa1	T	C	12: 55,809,359 (GRCm39)	D472G	possibly damaging	Het
Rexo4	A	G	2: 26,850,346 (GRCm39)	I277T	probably damaging	Het
Rimbp2	C	T	5: 128,865,579 (GRCm39)	V590I	probably benign	Het
Ripor3	A	G	2: 167,824,736 (GRCm39)	V864A	probably benign	Het
Rnpc3	G	A	3: 113,418,628 (GRCm39)	H107Y	possibly damaging	Het
Ryr1	A	T	7: 28,767,520 (GRCm39)	D2927E	probably damaging	Het
Samd9l	G	A	6: 3,375,621 (GRCm39)	Q547*	probably null	Het
Scaper	T	C	9: 55,716,459 (GRCm39)	E724G	probably damaging	Het
Secisbp2l	C	T	2: 125,582,409 (GRCm39)	V1016I	probably damaging	Het
Selenoo	T	A	15: 88,982,970 (GRCm39)	M499K	probably damaging	Het
Sema4a	T	A	3: 88,345,568 (GRCm39)	D505V	probably damaging	Het
Sema5a	T	C	15: 32,679,310 (GRCm39)	M863T	probably damaging	Het
Slc24a5	T	C	2: 124,929,940 (GRCm39)	C414R	probably damaging	Het
Slc34a2	A	G	5: 53,224,942 (GRCm39)	D361G	probably damaging	Het
Slc5a1	T	C	5: 33,261,858 (GRCm39)	Y20H	probably benign	Het
Slx4ip	A	T	2: 136,910,267 (GRCm39)		probably benign	Het
Snx14	T	A	9: 88,280,341 (GRCm39)	E538V	probably damaging	Het
St13	G	C	15: 81,283,786 (GRCm39)	R4G	probably benign	Het
Stap2	A	G	17: 56,304,901 (GRCm39)	S294P	possibly damaging	Het
Stat1	C	A	1: 52,193,082 (GRCm39)	Y651*	probably null	Het
Syne2	A	G	12: 75,946,046 (GRCm39)	T373A	probably benign	Het
Tacc2	A	G	7: 130,330,318 (GRCm39)	S201G	probably damaging	Het
Tdpoz3	G	A	3: 93,734,287 (GRCm39)	E321K	probably benign	Het
Thumpd1	T	C	7: 119,316,002 (GRCm39)	T316A	probably benign	Het
Tlr3	A	T	8: 45,850,072 (GRCm39)	C866S	probably benign	Het
Tmprss11g	T	A	5: 86,644,401 (GRCm39)	I148F	probably benign	Het
Topbp1	T	A	9: 103,205,568 (GRCm39)		probably benign	Het
Tpm1	T	C	9: 66,935,331 (GRCm39)		probably null	Het
Traf3ip2	T	A	10: 39,502,096 (GRCm39)	S81R	probably damaging	Het
Trim29	T	A	9: 43,222,265 (GRCm39)	N31K	probably benign	Het
Ubn2	C	T	6: 38,467,433 (GRCm39)	P563S	probably benign	Het
Usp24	T	C	4: 106,283,743 (GRCm39)	Y2418H	probably benign	Het
Vmn2r6	T	A	3: 64,463,766 (GRCm39)	D267V	probably damaging	Het
Wapl	T	A	14: 34,414,052 (GRCm39)	C305S	probably benign	Het
Yme1l1	C	A	2: 23,058,333 (GRCm39)	S155*	probably null	Het
Ythdf3	T	A	3: 16,258,220 (GRCm39)	H126Q	probably damaging	Het
Zbtb32	A	T	7: 30,290,678 (GRCm39)	C206S	possibly damaging	Het
Zbtb47	T	C	9: 121,593,045 (GRCm39)	V455A	probably damaging	Het
Zfp518a	A	T	19: 40,902,707 (GRCm39)	I879F	probably benign	Het
Zfp938	A	T	10: 82,062,012 (GRCm39)	Y203N	possibly damaging	Het

Other mutations in Sspo

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00333:Sspo	APN	6	48,447,387 (GRCm39)	missense	probably benign	0.02
IGL00339:Sspo	APN	6	48,460,680 (GRCm39)	splice site	probably benign
IGL00391:Sspo	APN	6	48,474,320 (GRCm39)	missense	probably damaging	0.96
IGL00433:Sspo	APN	6	48,466,970 (GRCm39)	missense	probably damaging	1.00
IGL00471:Sspo	APN	6	48,475,147 (GRCm39)	splice site	probably benign
IGL00500:Sspo	APN	6	48,474,355 (GRCm39)	nonsense	probably null
IGL00537:Sspo	APN	6	48,475,147 (GRCm39)	splice site	probably benign
IGL00540:Sspo	APN	6	48,475,147 (GRCm39)	splice site	probably benign
IGL01060:Sspo	APN	6	48,426,413 (GRCm39)	nonsense	probably null
IGL01090:Sspo	APN	6	48,467,059 (GRCm39)	missense	probably benign	0.08
IGL01125:Sspo	APN	6	48,469,822 (GRCm39)	missense	probably damaging	1.00
IGL01447:Sspo	APN	6	48,441,600 (GRCm39)	splice site	probably null
IGL01457:Sspo	APN	6	48,475,277 (GRCm39)	missense	probably benign	0.00
IGL01481:Sspo	APN	6	48,425,449 (GRCm39)	missense	probably benign	0.41
IGL01485:Sspo	APN	6	48,455,665 (GRCm39)	missense	probably damaging	1.00
IGL01544:Sspo	APN	6	48,467,953 (GRCm39)	missense	probably damaging	0.99
IGL01575:Sspo	APN	6	48,435,976 (GRCm39)	missense	probably benign	0.01
IGL01589:Sspo	APN	6	48,428,112 (GRCm39)	missense	probably damaging	1.00
IGL01601:Sspo	APN	6	48,463,313 (GRCm39)	missense	probably benign	0.33
IGL01644:Sspo	APN	6	48,429,436 (GRCm39)	missense	probably benign
IGL01659:Sspo	APN	6	48,451,377 (GRCm39)	missense	probably damaging	1.00
IGL01801:Sspo	APN	6	48,434,072 (GRCm39)	missense	probably damaging	1.00
IGL01872:Sspo	APN	6	48,431,623 (GRCm39)	missense	probably damaging	0.99
IGL01874:Sspo	APN	6	48,429,124 (GRCm39)	missense	probably damaging	1.00
IGL01936:Sspo	APN	6	48,452,821 (GRCm39)	missense	probably damaging	1.00
IGL01941:Sspo	APN	6	48,472,116 (GRCm39)	missense	probably benign	0.19
IGL01986:Sspo	APN	6	48,460,237 (GRCm39)	missense	probably benign	0.05
IGL01987:Sspo	APN	6	48,454,558 (GRCm39)	splice site	probably null
IGL02170:Sspo	APN	6	48,444,917 (GRCm39)	missense	possibly damaging	0.76
IGL02192:Sspo	APN	6	48,436,502 (GRCm39)	missense	possibly damaging	0.86
IGL02210:Sspo	APN	6	48,477,426 (GRCm39)	missense	probably damaging	1.00
IGL02225:Sspo	APN	6	48,461,268 (GRCm39)	missense	probably benign	0.09
IGL02280:Sspo	APN	6	48,473,165 (GRCm39)	missense	probably damaging	1.00
IGL02303:Sspo	APN	6	48,461,639 (GRCm39)	missense	possibly damaging	0.52
IGL02397:Sspo	APN	6	48,438,572 (GRCm39)	missense	probably benign	0.35
IGL02451:Sspo	APN	6	48,437,237 (GRCm39)	splice site	probably benign
IGL02500:Sspo	APN	6	48,455,313 (GRCm39)	nonsense	probably null
IGL02519:Sspo	APN	6	48,461,762 (GRCm39)	missense	probably damaging	1.00
IGL02549:Sspo	APN	6	48,428,707 (GRCm39)	missense	possibly damaging	0.81
IGL02562:Sspo	APN	6	48,467,056 (GRCm39)	splice site	probably null
IGL02673:Sspo	APN	6	48,475,709 (GRCm39)	critical splice donor site	probably null
IGL02673:Sspo	APN	6	48,452,794 (GRCm39)	missense	probably damaging	1.00
IGL02719:Sspo	APN	6	48,459,601 (GRCm39)	missense	probably benign	0.39
IGL02793:Sspo	APN	6	48,464,828 (GRCm39)	splice site	probably benign
IGL03003:Sspo	APN	6	48,432,021 (GRCm39)	missense	probably damaging	0.98
IGL03056:Sspo	APN	6	48,447,472 (GRCm39)	missense	probably benign	0.17
IGL03105:Sspo	APN	6	48,450,592 (GRCm39)	splice site	probably benign
IGL03116:Sspo	APN	6	48,471,035 (GRCm39)	missense	probably benign	0.32
IGL03163:Sspo	APN	6	48,461,266 (GRCm39)	missense	probably benign	0.19
IGL03198:Sspo	APN	6	48,454,516 (GRCm39)	missense	probably benign	0.31
IGL03365:Sspo	APN	6	48,436,349 (GRCm39)	missense	possibly damaging	0.82
Barrier	UTSW	6	48,472,146 (GRCm39)	missense	possibly damaging	0.58
R0312_sspo_280	UTSW	6	48,432,335 (GRCm39)	missense	possibly damaging	0.52
R3112_Sspo_731	UTSW	6	48,434,534 (GRCm39)	missense	probably damaging	1.00
R3498_Sspo_650	UTSW	6	48,444,914 (GRCm39)	missense	possibly damaging	0.58
R4180_Sspo_324	UTSW	6	48,475,329 (GRCm39)	critical splice donor site	probably null
spotsylvania	UTSW	6	48,453,505 (GRCm39)	nonsense	probably null
ANU74:Sspo	UTSW	6	48,437,893 (GRCm39)	missense	probably damaging	1.00
IGL02984:Sspo	UTSW	6	48,472,089 (GRCm39)	missense	probably benign	0.33
IGL03052:Sspo	UTSW	6	48,437,387 (GRCm39)	missense	probably damaging	1.00
IGL03134:Sspo	UTSW	6	48,427,999 (GRCm39)	missense	probably benign	0.28
PIT4531001:Sspo	UTSW	6	48,458,173 (GRCm39)	missense	probably benign
R0087:Sspo	UTSW	6	48,454,719 (GRCm39)	missense	probably damaging	1.00
R0122:Sspo	UTSW	6	48,450,910 (GRCm39)	missense	possibly damaging	0.95
R0129:Sspo	UTSW	6	48,432,352 (GRCm39)	missense	probably benign	0.00
R0164:Sspo	UTSW	6	48,471,128 (GRCm39)	splice site	probably benign
R0195:Sspo	UTSW	6	48,463,570 (GRCm39)	missense	probably benign
R0200:Sspo	UTSW	6	48,463,349 (GRCm39)	missense	probably null	0.01
R0201:Sspo	UTSW	6	48,432,686 (GRCm39)	missense	possibly damaging	0.64
R0241:Sspo	UTSW	6	48,438,429 (GRCm39)	missense	possibly damaging	0.82
R0241:Sspo	UTSW	6	48,438,429 (GRCm39)	missense	possibly damaging	0.82
R0243:Sspo	UTSW	6	48,470,120 (GRCm39)	missense	probably damaging	1.00
R0268:Sspo	UTSW	6	48,442,489 (GRCm39)	missense	probably benign	0.26
R0312:Sspo	UTSW	6	48,432,335 (GRCm39)	missense	possibly damaging	0.52
R0449:Sspo	UTSW	6	48,443,674 (GRCm39)	missense	probably damaging	1.00
R0523:Sspo	UTSW	6	48,428,794 (GRCm39)	missense	probably benign	0.20
R0576:Sspo	UTSW	6	48,441,876 (GRCm39)	splice site	probably null
R0671:Sspo	UTSW	6	48,467,325 (GRCm39)	splice site	probably benign
R0828:Sspo	UTSW	6	48,475,668 (GRCm39)	missense	probably damaging	1.00
R0880:Sspo	UTSW	6	48,452,869 (GRCm39)	missense	possibly damaging	0.69
R0903:Sspo	UTSW	6	48,432,242 (GRCm39)	critical splice acceptor site	probably null
R1051:Sspo	UTSW	6	48,468,389 (GRCm39)	nonsense	probably null
R1083:Sspo	UTSW	6	48,447,933 (GRCm39)	missense	possibly damaging	0.91
R1109:Sspo	UTSW	6	48,474,377 (GRCm39)	missense	probably damaging	1.00
R1118:Sspo	UTSW	6	48,436,352 (GRCm39)	missense	probably damaging	0.97
R1256:Sspo	UTSW	6	48,434,573 (GRCm39)	missense	probably damaging	1.00
R1342:Sspo	UTSW	6	48,438,569 (GRCm39)	missense	probably benign	0.07
R1355:Sspo	UTSW	6	48,425,560 (GRCm39)	missense	probably benign	0.41
R1370:Sspo	UTSW	6	48,425,560 (GRCm39)	missense	probably benign	0.41
R1469:Sspo	UTSW	6	48,467,916 (GRCm39)	missense	probably damaging	1.00
R1469:Sspo	UTSW	6	48,467,916 (GRCm39)	missense	probably damaging	1.00
R1476:Sspo	UTSW	6	48,440,334 (GRCm39)	critical splice donor site	probably null
R1566:Sspo	UTSW	6	48,443,804 (GRCm39)	critical splice donor site	probably null
R1630:Sspo	UTSW	6	48,434,658 (GRCm39)	missense	probably benign	0.01
R1686:Sspo	UTSW	6	48,437,334 (GRCm39)	missense	probably benign	0.00
R1707:Sspo	UTSW	6	48,454,811 (GRCm39)	missense	probably damaging	0.99
R1727:Sspo	UTSW	6	48,471,782 (GRCm39)	missense	probably damaging	1.00
R1822:Sspo	UTSW	6	48,469,820 (GRCm39)	missense	possibly damaging	0.75
R1831:Sspo	UTSW	6	48,466,720 (GRCm39)	missense	probably damaging	1.00
R1835:Sspo	UTSW	6	48,434,274 (GRCm39)	missense	probably damaging	0.97
R1862:Sspo	UTSW	6	48,467,940 (GRCm39)	missense	probably damaging	0.98
R1878:Sspo	UTSW	6	48,436,300 (GRCm39)	missense	possibly damaging	0.92
R1900:Sspo	UTSW	6	48,436,284 (GRCm39)	missense	probably benign	0.22
R1945:Sspo	UTSW	6	48,466,707 (GRCm39)	missense	possibly damaging	0.93
R1957:Sspo	UTSW	6	48,455,207 (GRCm39)	missense	probably damaging	0.99
R1990:Sspo	UTSW	6	48,427,984 (GRCm39)	missense	probably benign	0.00
R1996:Sspo	UTSW	6	48,452,424 (GRCm39)	missense	possibly damaging	0.50
R2049:Sspo	UTSW	6	48,440,465 (GRCm39)	missense	probably benign	0.36
R2049:Sspo	UTSW	6	48,437,697 (GRCm39)	splice site	probably benign
R2064:Sspo	UTSW	6	48,450,596 (GRCm39)	missense	probably damaging	0.99
R2072:Sspo	UTSW	6	48,450,451 (GRCm39)	missense	probably benign	0.01
R2096:Sspo	UTSW	6	48,438,608 (GRCm39)	missense	probably benign
R2106:Sspo	UTSW	6	48,443,250 (GRCm39)	missense	possibly damaging	0.96
R2230:Sspo	UTSW	6	48,477,437 (GRCm39)	missense	probably benign	0.11
R2230:Sspo	UTSW	6	48,425,606 (GRCm39)	missense	probably damaging	0.97
R2232:Sspo	UTSW	6	48,425,606 (GRCm39)	missense	probably damaging	0.97
R2351:Sspo	UTSW	6	48,441,803 (GRCm39)	missense	probably damaging	1.00
R2423:Sspo	UTSW	6	48,430,989 (GRCm39)	missense	probably benign	0.00
R2508:Sspo	UTSW	6	48,441,298 (GRCm39)	missense	probably damaging	1.00
R3110:Sspo	UTSW	6	48,434,534 (GRCm39)	missense	probably damaging	1.00
R3112:Sspo	UTSW	6	48,434,534 (GRCm39)	missense	probably damaging	1.00
R3413:Sspo	UTSW	6	48,457,631 (GRCm39)	missense	probably damaging	1.00
R3433:Sspo	UTSW	6	48,452,885 (GRCm39)	splice site	probably null
R3498:Sspo	UTSW	6	48,444,914 (GRCm39)	missense	possibly damaging	0.58
R3732:Sspo	UTSW	6	48,426,864 (GRCm39)	missense	probably damaging	1.00
R3816:Sspo	UTSW	6	48,458,037 (GRCm39)	missense	possibly damaging	0.77
R3818:Sspo	UTSW	6	48,458,037 (GRCm39)	missense	possibly damaging	0.77
R3819:Sspo	UTSW	6	48,458,037 (GRCm39)	missense	possibly damaging	0.77
R3838:Sspo	UTSW	6	48,457,754 (GRCm39)	missense	probably damaging	1.00
R3850:Sspo	UTSW	6	48,469,424 (GRCm39)	missense	probably damaging	1.00
R3880:Sspo	UTSW	6	48,471,874 (GRCm39)	missense	probably benign	0.38
R3893:Sspo	UTSW	6	48,453,505 (GRCm39)	nonsense	probably null
R4116:Sspo	UTSW	6	48,433,928 (GRCm39)	missense	probably damaging	0.99
R4179:Sspo	UTSW	6	48,475,329 (GRCm39)	critical splice donor site	probably null
R4180:Sspo	UTSW	6	48,475,329 (GRCm39)	critical splice donor site	probably null
R4207:Sspo	UTSW	6	48,455,227 (GRCm39)	missense	probably benign	0.00
R4210:Sspo	UTSW	6	48,441,835 (GRCm39)	missense	probably benign	0.00
R4223:Sspo	UTSW	6	48,428,091 (GRCm39)	missense	possibly damaging	0.54
R4224:Sspo	UTSW	6	48,428,091 (GRCm39)	missense	possibly damaging	0.54
R4225:Sspo	UTSW	6	48,428,091 (GRCm39)	missense	possibly damaging	0.54
R4229:Sspo	UTSW	6	48,467,868 (GRCm39)	missense	probably benign	0.00
R4230:Sspo	UTSW	6	48,467,868 (GRCm39)	missense	probably benign	0.00
R4363:Sspo	UTSW	6	48,475,665 (GRCm39)	missense	probably damaging	1.00
R4370:Sspo	UTSW	6	48,443,282 (GRCm39)	missense	probably null	0.14
R4407:Sspo	UTSW	6	48,437,454 (GRCm39)	missense	probably damaging	1.00
R4438:Sspo	UTSW	6	48,464,287 (GRCm39)	missense	probably damaging	1.00
R4454:Sspo	UTSW	6	48,464,159 (GRCm39)	missense	probably benign	0.05
R4455:Sspo	UTSW	6	48,442,450 (GRCm39)	missense	probably damaging	1.00
R4561:Sspo	UTSW	6	48,452,468 (GRCm39)	splice site	probably null
R4574:Sspo	UTSW	6	48,442,457 (GRCm39)	missense	probably damaging	1.00
R4578:Sspo	UTSW	6	48,440,307 (GRCm39)	missense	possibly damaging	0.58
R4653:Sspo	UTSW	6	48,455,580 (GRCm39)	missense	probably damaging	1.00
R4656:Sspo	UTSW	6	48,431,010 (GRCm39)	missense	possibly damaging	0.65
R4659:Sspo	UTSW	6	48,461,147 (GRCm39)	missense	probably damaging	1.00
R4664:Sspo	UTSW	6	48,450,468 (GRCm39)	missense	possibly damaging	0.82
R4685:Sspo	UTSW	6	48,469,828 (GRCm39)	missense	probably damaging	0.98
R4692:Sspo	UTSW	6	48,459,621 (GRCm39)	missense	probably damaging	1.00
R4703:Sspo	UTSW	6	48,477,387 (GRCm39)	missense	probably damaging	1.00
R4704:Sspo	UTSW	6	48,475,638 (GRCm39)	missense	probably damaging	1.00
R4738:Sspo	UTSW	6	48,455,330 (GRCm39)	missense	possibly damaging	0.78
R4766:Sspo	UTSW	6	48,447,514 (GRCm39)	missense	probably benign	0.04
R4771:Sspo	UTSW	6	48,437,813 (GRCm39)	missense	probably damaging	1.00
R4790:Sspo	UTSW	6	48,437,705 (GRCm39)	missense	probably benign	0.04
R4792:Sspo	UTSW	6	48,438,519 (GRCm39)	missense	probably benign	0.00
R4808:Sspo	UTSW	6	48,428,095 (GRCm39)	missense	probably damaging	1.00
R4812:Sspo	UTSW	6	48,467,444 (GRCm39)	missense	probably benign	0.00
R4883:Sspo	UTSW	6	48,437,756 (GRCm39)	missense	probably benign	0.00
R4906:Sspo	UTSW	6	48,442,664 (GRCm39)	critical splice acceptor site	probably null
R4945:Sspo	UTSW	6	48,444,021 (GRCm39)	splice site	probably null
R4967:Sspo	UTSW	6	48,441,539 (GRCm39)	missense	probably damaging	0.97
R5016:Sspo	UTSW	6	48,429,214 (GRCm39)	nonsense	probably null
R5018:Sspo	UTSW	6	48,432,634 (GRCm39)	missense	probably damaging	1.00
R5034:Sspo	UTSW	6	48,457,757 (GRCm39)	missense	possibly damaging	0.93
R5044:Sspo	UTSW	6	48,443,889 (GRCm39)	critical splice acceptor site	probably null
R5055:Sspo	UTSW	6	48,441,729 (GRCm39)	missense	probably damaging	1.00
R5087:Sspo	UTSW	6	48,465,405 (GRCm39)	missense	possibly damaging	0.51
R5155:Sspo	UTSW	6	48,437,408 (GRCm39)	missense	probably benign	0.03
R5223:Sspo	UTSW	6	48,455,258 (GRCm39)	missense	probably damaging	1.00
R5249:Sspo	UTSW	6	48,470,244 (GRCm39)	missense	probably damaging	0.98
R5257:Sspo	UTSW	6	48,453,428 (GRCm39)	missense	probably damaging	1.00
R5258:Sspo	UTSW	6	48,453,428 (GRCm39)	missense	probably damaging	1.00
R5276:Sspo	UTSW	6	48,467,401 (GRCm39)	missense	probably damaging	1.00
R5307:Sspo	UTSW	6	48,431,784 (GRCm39)	missense	probably damaging	0.99
R5341:Sspo	UTSW	6	48,436,549 (GRCm39)	missense	probably damaging	1.00
R5361:Sspo	UTSW	6	48,443,247 (GRCm39)	missense	probably benign	0.02
R5385:Sspo	UTSW	6	48,439,187 (GRCm39)	missense	probably benign	0.18
R5394:Sspo	UTSW	6	48,472,194 (GRCm39)	missense	possibly damaging	0.52
R5477:Sspo	UTSW	6	48,475,327 (GRCm39)	missense	possibly damaging	0.60
R5490:Sspo	UTSW	6	48,470,214 (GRCm39)	missense	probably benign	0.33
R5512:Sspo	UTSW	6	48,432,605 (GRCm39)	missense	probably damaging	0.97
R5518:Sspo	UTSW	6	48,473,588 (GRCm39)	missense	possibly damaging	0.92
R5530:Sspo	UTSW	6	48,442,517 (GRCm39)	missense	probably damaging	0.97
R5538:Sspo	UTSW	6	48,429,112 (GRCm39)	missense	probably damaging	0.99
R5590:Sspo	UTSW	6	48,451,425 (GRCm39)	missense	probably damaging	1.00
R5613:Sspo	UTSW	6	48,431,978 (GRCm39)	missense	possibly damaging	0.79
R5638:Sspo	UTSW	6	48,469,825 (GRCm39)	missense	possibly damaging	0.86
R5809:Sspo	UTSW	6	48,436,979 (GRCm39)	missense	possibly damaging	0.59
R5810:Sspo	UTSW	6	48,460,832 (GRCm39)	missense	probably benign	0.02
R5814:Sspo	UTSW	6	48,428,818 (GRCm39)	missense	probably damaging	1.00
R5915:Sspo	UTSW	6	48,468,418 (GRCm39)	missense	possibly damaging	0.83
R5915:Sspo	UTSW	6	48,441,530 (GRCm39)	missense	probably benign	0.00
R5979:Sspo	UTSW	6	48,440,627 (GRCm39)	missense	probably benign	0.20
R5996:Sspo	UTSW	6	48,471,110 (GRCm39)	missense	possibly damaging	0.87
R6012:Sspo	UTSW	6	48,428,305 (GRCm39)	missense	probably benign	0.00
R6025:Sspo	UTSW	6	48,463,720 (GRCm39)	missense	possibly damaging	0.83
R6120:Sspo	UTSW	6	48,442,510 (GRCm39)	missense	probably damaging	1.00
R6150:Sspo	UTSW	6	48,463,313 (GRCm39)	missense	probably benign	0.33
R6221:Sspo	UTSW	6	48,440,639 (GRCm39)	missense	probably damaging	1.00
R6261:Sspo	UTSW	6	48,439,125 (GRCm39)	missense	possibly damaging	0.75
R6312:Sspo	UTSW	6	48,434,300 (GRCm39)	critical splice donor site	probably null
R6372:Sspo	UTSW	6	48,449,475 (GRCm39)	missense	probably damaging	1.00
R6456:Sspo	UTSW	6	48,428,740 (GRCm39)	missense	probably benign	0.08
R6497:Sspo	UTSW	6	48,472,142 (GRCm39)	missense	possibly damaging	0.71
R6501:Sspo	UTSW	6	48,472,146 (GRCm39)	missense	possibly damaging	0.58
R6617:Sspo	UTSW	6	48,467,980 (GRCm39)	missense	possibly damaging	0.93
R6825:Sspo	UTSW	6	48,442,459 (GRCm39)	missense	probably benign	0.04
R6831:Sspo	UTSW	6	48,461,767 (GRCm39)	missense	possibly damaging	0.68
R6861:Sspo	UTSW	6	48,464,889 (GRCm39)	missense	probably benign	0.15
R6961:Sspo	UTSW	6	48,440,811 (GRCm39)	missense	probably benign	0.05
R6967:Sspo	UTSW	6	48,466,728 (GRCm39)	missense	probably benign	0.21
R7016:Sspo	UTSW	6	48,426,098 (GRCm39)	missense	probably damaging	1.00
R7035:Sspo	UTSW	6	48,426,147 (GRCm39)	splice site	probably null
R7058:Sspo	UTSW	6	48,425,516 (GRCm39)	missense	probably damaging	1.00
R7072:Sspo	UTSW	6	48,431,913 (GRCm39)	missense	probably damaging	1.00
R7078:Sspo	UTSW	6	48,437,313 (GRCm39)	missense	probably damaging	1.00
R7082:Sspo	UTSW	6	48,455,543 (GRCm39)	critical splice acceptor site	probably null
R7120:Sspo	UTSW	6	48,442,505 (GRCm39)	missense	probably benign	0.05
R7127:Sspo	UTSW	6	48,426,446 (GRCm39)	missense	probably benign	0.02
R7146:Sspo	UTSW	6	48,478,029 (GRCm39)	missense	probably benign	0.15
R7220:Sspo	UTSW	6	48,453,540 (GRCm39)	nonsense	probably null
R7242:Sspo	UTSW	6	48,450,886 (GRCm39)	missense	probably benign
R7261:Sspo	UTSW	6	48,427,011 (GRCm39)	missense	possibly damaging	0.52
R7313:Sspo	UTSW	6	48,450,390 (GRCm39)	missense	probably benign	0.04
R7313:Sspo	UTSW	6	48,431,762 (GRCm39)	missense	probably damaging	1.00
R7323:Sspo	UTSW	6	48,438,581 (GRCm39)	missense	possibly damaging	0.93
R7330:Sspo	UTSW	6	48,452,396 (GRCm39)	missense	probably benign	0.00
R7351:Sspo	UTSW	6	48,441,855 (GRCm39)	missense	possibly damaging	0.89
R7467:Sspo	UTSW	6	48,463,237 (GRCm39)	missense	probably damaging	1.00
R7475:Sspo	UTSW	6	48,432,794 (GRCm39)	missense	probably benign	0.37
R7489:Sspo	UTSW	6	48,450,647 (GRCm39)	missense	probably damaging	0.99
R7508:Sspo	UTSW	6	48,443,633 (GRCm39)	missense	probably damaging	1.00
R7515:Sspo	UTSW	6	48,470,820 (GRCm39)	missense	probably damaging	1.00
R7564:Sspo	UTSW	6	48,426,434 (GRCm39)	missense	probably benign	0.04
R7607:Sspo	UTSW	6	48,466,661 (GRCm39)	missense	probably damaging	1.00
R7620:Sspo	UTSW	6	48,444,020 (GRCm39)	critical splice donor site	probably null
R7667:Sspo	UTSW	6	48,452,305 (GRCm39)	nonsense	probably null
R7691:Sspo	UTSW	6	48,461,163 (GRCm39)	missense	probably benign	0.12
R7707:Sspo	UTSW	6	48,438,461 (GRCm39)	missense	probably benign	0.01
R7723:Sspo	UTSW	6	48,441,572 (GRCm39)	missense	probably damaging	0.99
R7748:Sspo	UTSW	6	48,426,399 (GRCm39)	nonsense	probably null
R7767:Sspo	UTSW	6	48,428,316 (GRCm39)	missense	probably damaging	0.96
R7792:Sspo	UTSW	6	48,431,624 (GRCm39)	missense	probably damaging	0.98
R7878:Sspo	UTSW	6	48,469,460 (GRCm39)	missense	probably damaging	1.00
R7893:Sspo	UTSW	6	48,440,244 (GRCm39)	missense	probably benign	0.02
R7942:Sspo	UTSW	6	48,465,434 (GRCm39)	splice site	probably null
R7952:Sspo	UTSW	6	48,464,263 (GRCm39)	missense	probably damaging	1.00
R7981:Sspo	UTSW	6	48,445,428 (GRCm39)	missense	probably benign
R7995:Sspo	UTSW	6	48,469,823 (GRCm39)	missense	probably damaging	1.00
R8088:Sspo	UTSW	6	48,434,547 (GRCm39)	missense	probably damaging	1.00
R8129:Sspo	UTSW	6	48,443,959 (GRCm39)	missense	possibly damaging	0.79
R8145:Sspo	UTSW	6	48,444,683 (GRCm39)	missense	possibly damaging	0.49
R8202:Sspo	UTSW	6	48,434,534 (GRCm39)	missense	probably damaging	1.00
R8211:Sspo	UTSW	6	48,469,543 (GRCm39)	critical splice donor site	probably null
R8240:Sspo	UTSW	6	48,460,436 (GRCm39)	missense	possibly damaging	0.84
R8252:Sspo	UTSW	6	48,462,386 (GRCm39)	missense	probably damaging	0.99
R8270:Sspo	UTSW	6	48,426,897 (GRCm39)	missense	probably benign
R8272:Sspo	UTSW	6	48,425,453 (GRCm39)	missense	probably benign	0.03
R8316:Sspo	UTSW	6	48,459,622 (GRCm39)	missense	probably damaging	1.00
R8384:Sspo	UTSW	6	48,459,598 (GRCm39)	missense	probably damaging	1.00
R8390:Sspo	UTSW	6	48,444,896 (GRCm39)	missense	probably benign	0.00
R8770:Sspo	UTSW	6	48,451,206 (GRCm39)	missense	probably null	1.00
R8827:Sspo	UTSW	6	48,434,606 (GRCm39)	missense	possibly damaging	0.59
R8882:Sspo	UTSW	6	48,452,390 (GRCm39)	missense	probably damaging	1.00
R8886:Sspo	UTSW	6	48,458,201 (GRCm39)	missense	possibly damaging	0.92
R8946:Sspo	UTSW	6	48,434,071 (GRCm39)	missense	probably damaging	1.00
R8947:Sspo	UTSW	6	48,425,504 (GRCm39)	missense	probably damaging	1.00
R9028:Sspo	UTSW	6	48,473,087 (GRCm39)	missense	probably benign	0.38
R9043:Sspo	UTSW	6	48,470,214 (GRCm39)	missense	probably benign	0.07
R9056:Sspo	UTSW	6	48,450,608 (GRCm39)	missense	probably damaging	0.97
R9071:Sspo	UTSW	6	48,433,982 (GRCm39)	missense	probably benign	0.00
R9133:Sspo	UTSW	6	48,434,747 (GRCm39)	missense	possibly damaging	0.81
R9187:Sspo	UTSW	6	48,472,223 (GRCm39)	missense	probably damaging	1.00
R9205:Sspo	UTSW	6	48,432,806 (GRCm39)	missense	probably benign	0.03
R9213:Sspo	UTSW	6	48,440,869 (GRCm39)	missense	possibly damaging	0.91
R9214:Sspo	UTSW	6	48,440,869 (GRCm39)	missense	possibly damaging	0.91
R9215:Sspo	UTSW	6	48,440,869 (GRCm39)	missense	possibly damaging	0.91
R9235:Sspo	UTSW	6	48,466,718 (GRCm39)	missense	probably damaging	1.00
R9254:Sspo	UTSW	6	48,464,928 (GRCm39)	missense	probably damaging	1.00
R9291:Sspo	UTSW	6	48,473,330 (GRCm39)	missense	probably damaging	1.00
R9312:Sspo	UTSW	6	48,445,396 (GRCm39)	missense	probably benign	0.00
R9357:Sspo	UTSW	6	48,443,989 (GRCm39)	missense	possibly damaging	0.77
R9480:Sspo	UTSW	6	48,470,820 (GRCm39)	missense	probably damaging	1.00
R9586:Sspo	UTSW	6	48,458,039 (GRCm39)	missense	probably benign	0.03
R9660:Sspo	UTSW	6	48,432,707 (GRCm39)	missense	probably damaging	1.00
R9661:Sspo	UTSW	6	48,455,272 (GRCm39)	nonsense	probably null
R9728:Sspo	UTSW	6	48,432,707 (GRCm39)	missense	probably damaging	1.00
R9776:Sspo	UTSW	6	48,439,269 (GRCm39)	missense	probably benign	0.00
RF009:Sspo	UTSW	6	48,436,919 (GRCm39)	nonsense	probably null
X0060:Sspo	UTSW	6	48,457,728 (GRCm39)	missense	probably damaging	1.00
X0060:Sspo	UTSW	6	48,443,228 (GRCm39)	missense	probably damaging	1.00
X0063:Sspo	UTSW	6	48,474,356 (GRCm39)	missense	probably damaging	0.96
X0065:Sspo	UTSW	6	48,438,618 (GRCm39)	missense	probably benign	0.00
Z1176:Sspo	UTSW	6	48,458,227 (GRCm39)	missense	probably damaging	1.00
Z1177:Sspo	UTSW	6	48,467,824 (GRCm39)	missense	probably damaging	1.00
Z1177:Sspo	UTSW	6	48,467,482 (GRCm39)	nonsense	probably null
Z1177:Sspo	UTSW	6	48,450,369 (GRCm39)	missense	probably damaging	0.99
Z1177:Sspo	UTSW	6	48,447,918 (GRCm39)	missense	probably benign	0.16
Z1177:Sspo	UTSW	6	48,441,750 (GRCm39)	missense	possibly damaging	0.72
Z1177:Sspo	UTSW	6	48,433,960 (GRCm39)	missense	probably benign	0.31
Z1186:Sspo	UTSW	6	48,445,441 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ATGCCCTTTGTCCACCAAGC -3'
(R):5'- GGCAACATGATTCTCCCATCCC -3'

Sequencing Primer
(F):5'- AAGCAGGGTGGAGGTAGCTTTG -3'
(R):5'- CAGGACCCAGCCCTGTG -3'

Posted On

2016-04-15