Mutagenetix > Incidental Mutation

Incidental Mutation 'R4938:Mettl3'

382865

Institutional Source

Beutler Lab

Gene Symbol

Mettl3

Ensembl Gene

ENSMUSG00000022160

Gene Name

methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit

Synonyms

M6A, 2310024F18Rik, Spo8

MMRRC Submission

042537-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4938 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52532298-52542585 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 52537184 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 182 (S182T)

Ref Sequence

ENSEMBL: ENSMUSP00000134732 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022766] [ENSMUST00000022767] [ENSMUST00000122962] [ENSMUST00000145875] [ENSMUST00000147768] [ENSMUST00000174853] [ENSMUST00000174351] [ENSMUST00000173896] [ENSMUST00000173138]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000022766

SMART Domains

Protein: ENSMUSP00000022766
Gene: ENSMUSG00000016831

Domain	Start	End	E-Value	Type
low complexity region	146	160	N/A	INTRINSIC
low complexity region	207	218	N/A	INTRINSIC
HMG	222	292	1.17e-18	SMART
low complexity region	307	339	N/A	INTRINSIC
low complexity region	435	476	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000022767
AA Change: S233T

PolyPhen 2 Score 0.444 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000022767
Gene: ENSMUSG00000022160
AA Change: S233T

Domain	Start	End	E-Value	Type
low complexity region	53	67	N/A	INTRINSIC
low complexity region	82	93	N/A	INTRINSIC
low complexity region	191	213	N/A	INTRINSIC
Pfam:MT-A70	389	550	9.9e-65	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000122962

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127797

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130550

Predicted Effect

probably benign
Transcript: ENSMUST00000145875

Predicted Effect

unknown
Transcript: ENSMUST00000147768
AA Change: S233T

SMART Domains

Protein: ENSMUSP00000134577
Gene: ENSMUSG00000022160
AA Change: S233T

Domain	Start	End	E-Value	Type
low complexity region	53	67	N/A	INTRINSIC
low complexity region	82	93	N/A	INTRINSIC
low complexity region	191	213	N/A	INTRINSIC
low complexity region	231	242	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000174853
AA Change: S162T

SMART Domains

Protein: ENSMUSP00000133864
Gene: ENSMUSG00000022160
AA Change: S162T

Domain	Start	End	E-Value	Type
low complexity region	120	142	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000174351
AA Change: S182T

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000134732
Gene: ENSMUSG00000022160
AA Change: S182T

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	31	42	N/A	INTRINSIC
low complexity region	140	162	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197394

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156611

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173546

Predicted Effect

probably benign
Transcript: ENSMUST00000173896

SMART Domains

Protein: ENSMUSP00000133506
Gene: ENSMUSG00000022160

Domain	Start	End	E-Value	Type
low complexity region	53	67	N/A	INTRINSIC
low complexity region	82	93	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000173656

SMART Domains

Protein: ENSMUSP00000133759
Gene: ENSMUSG00000022160

Domain	Start	End	E-Value	Type
Pfam:MT-A70	1	60	8.6e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174360

SMART Domains

Protein: ENSMUSP00000134578
Gene: ENSMUSG00000022160

Domain	Start	End	E-Value	Type
Pfam:MT-A70	1	34	4.3e-10	PFAM
Pfam:MT-A70	30	74	1.4e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000173138

SMART Domains

Protein: ENSMUSP00000134018
Gene: ENSMUSG00000022160

Domain	Start	End	E-Value	Type
low complexity region	53	67	N/A	INTRINSIC
low complexity region	82	93	N/A	INTRINSIC

Meta Mutation Damage Score

0.0628

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

99% (74/75)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality between E3.5 and E8.5 with a deficiency in adopting the epiblast egg cylinder. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arl14ep	G	T	2: 106,799,663 (GRCm39)	H59Q	probably damaging	Het
Atp6v0a4	T	A	6: 38,055,749 (GRCm39)	I321F	possibly damaging	Het
Cdr2l	A	G	11: 115,284,651 (GRCm39)	D329G	possibly damaging	Het
Cep55	A	G	19: 38,058,364 (GRCm39)	E319G	probably damaging	Het
Cfhr2	C	T	1: 139,741,265 (GRCm39)	V237I	probably benign	Het
Clasrp	A	G	7: 19,318,703 (GRCm39)		probably null	Het
Col12a1	C	A	9: 79,607,632 (GRCm39)	E399*	probably null	Het
Cyp19a1	T	A	9: 54,080,647 (GRCm39)	I237F	probably benign	Het
Dnajc6	T	G	4: 101,494,010 (GRCm39)	N847K	probably damaging	Het
E330013P04Rik	C	G	19: 60,150,453 (GRCm39)		noncoding transcript	Het
Entpd2	A	G	2: 25,289,429 (GRCm39)	T304A	probably benign	Het
Etl4	A	G	2: 20,803,460 (GRCm39)	T779A	probably benign	Het
Fam107b	A	T	2: 3,773,907 (GRCm39)	I35L	probably benign	Het
Fgg	A	T	3: 82,920,175 (GRCm39)	Y318F	probably benign	Het
Fitm1	A	C	14: 55,814,076 (GRCm39)	T191P	probably damaging	Het
Fras1	A	G	5: 96,924,583 (GRCm39)	M3675V	probably damaging	Het
Fry	C	T	5: 150,401,454 (GRCm39)	R731W	probably damaging	Het
Galnt17	A	T	5: 131,335,237 (GRCm39)	S68T	probably benign	Het
Glp2r	G	T	11: 67,648,419 (GRCm39)	Y94*	probably null	Het
Grm1	C	T	10: 10,812,257 (GRCm39)	A256T	probably damaging	Het
Hoxd13	A	G	2: 74,499,027 (GRCm39)	Y125C	probably benign	Het
Hspa14	A	T	2: 3,492,646 (GRCm39)	I373K	probably benign	Het
Ifnlr1	A	G	4: 135,432,593 (GRCm39)	E343G	probably benign	Het
Ighv5-9	A	G	12: 113,625,582 (GRCm39)	S54P	probably benign	Het
Insyn2b	G	A	11: 34,352,231 (GRCm39)	G91E	probably damaging	Het
Irf2bpl	A	G	12: 86,928,892 (GRCm39)	S594P	possibly damaging	Het
Kcnq2	A	G	2: 180,728,766 (GRCm39)	S548P	probably damaging	Het
Lrp2	G	A	2: 69,302,712 (GRCm39)	R3006W	probably damaging	Het
Lrrc4c	A	T	2: 97,459,646 (GRCm39)	I91F	probably damaging	Het
Mdh1b	T	C	1: 63,750,663 (GRCm39)	D435G	probably benign	Het
Mllt6	A	G	11: 97,569,233 (GRCm39)	T862A	probably benign	Het
Mmp27	G	T	9: 7,578,983 (GRCm39)	R412I	probably damaging	Het
Mstn	A	G	1: 53,105,582 (GRCm39)	N308S	possibly damaging	Het
Ncapg	A	G	5: 45,828,551 (GRCm39)	T101A	probably benign	Het
Ngf	C	T	3: 102,427,790 (GRCm39)	R180W	probably damaging	Het
Notch1	G	A	2: 26,364,136 (GRCm39)	Q862*	probably null	Het
Nsrp1	T	C	11: 76,936,570 (GRCm39)	D542G	probably damaging	Het
Nup214	C	A	2: 31,873,171 (GRCm39)	T255K	probably benign	Het
Or5b21	T	A	19: 12,839,916 (GRCm39)	M259K	probably damaging	Het
Or8b43	T	A	9: 38,360,679 (GRCm39)	D170E	probably benign	Het
Papln	C	T	12: 83,829,677 (GRCm39)	P911S	probably benign	Het
Pdxdc1	A	G	16: 13,693,933 (GRCm39)	V163A	probably benign	Het
Plekhj1	A	T	10: 80,633,609 (GRCm39)	I76N	probably damaging	Het
Pnp2	A	G	14: 51,201,025 (GRCm39)		probably null	Het
Polr3gl	T	C	3: 96,487,208 (GRCm39)	E89G	probably benign	Het
Polrmt	A	G	10: 79,582,385 (GRCm39)	M1T	probably null	Het
Prss37	A	G	6: 40,491,917 (GRCm39)	I221T	possibly damaging	Het
Ptx4	C	T	17: 25,342,139 (GRCm39)	Q205*	probably null	Het
Qsox1	T	C	1: 155,655,414 (GRCm39)	E583G	probably benign	Het
Riok3	T	A	18: 12,288,300 (GRCm39)	N492K	probably benign	Het
Sec13	A	G	6: 113,712,153 (GRCm39)	W61R	probably damaging	Het
Slc20a2	T	C	8: 23,051,221 (GRCm39)	V418A	possibly damaging	Het
Smad9	G	A	3: 54,696,651 (GRCm39)	V239I	probably benign	Het
Stmn2	A	G	3: 8,610,792 (GRCm39)	E92G	probably damaging	Het
Taf1c	A	G	8: 120,325,537 (GRCm39)	V775A	probably benign	Het
Thsd7a	T	A	6: 12,330,991 (GRCm39)	I1384L	probably benign	Het
Tnxb	A	G	17: 34,932,606 (GRCm39)	Y2275C	probably damaging	Het
Trmo	G	T	4: 46,382,388 (GRCm39)	T243N	probably benign	Het
Tyrp1	C	T	4: 80,758,883 (GRCm39)	A252V	probably damaging	Het
Vasp	T	C	7: 18,991,642 (GRCm39)	*376W	probably null	Het
Vmn1r231	T	C	17: 21,110,613 (GRCm39)	I101V	possibly damaging	Het
Zfp532	T	C	18: 65,756,837 (GRCm39)	S257P	probably benign	Het
Zfp563	G	A	17: 33,324,683 (GRCm39)	C426Y	probably damaging	Het
Zfp647	TGCACG	TGCACGCACG	15: 76,795,244 (GRCm39)		probably null	Het
Zfp703	C	A	8: 27,469,801 (GRCm39)	H488Q	probably damaging	Het
Zfp964	A	G	8: 70,116,758 (GRCm39)	N452D	possibly damaging	Het
Zfyve28	A	C	5: 34,390,698 (GRCm39)	Y188D	probably damaging	Het

Other mutations in Mettl3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00401:Mettl3	APN	14	52,534,424 (GRCm39)	unclassified	probably benign
IGL00508:Mettl3	APN	14	52,532,436 (GRCm39)	unclassified	probably benign
R0417:Mettl3	UTSW	14	52,534,155 (GRCm39)	missense	probably damaging	1.00
R1533:Mettl3	UTSW	14	52,534,385 (GRCm39)	missense	probably benign	0.01
R2113:Mettl3	UTSW	14	52,532,441 (GRCm39)	makesense	probably null
R3785:Mettl3	UTSW	14	52,537,363 (GRCm39)	missense	probably benign	0.15
R3786:Mettl3	UTSW	14	52,537,363 (GRCm39)	missense	probably benign	0.15
R4651:Mettl3	UTSW	14	52,532,549 (GRCm39)	missense	probably damaging	1.00
R4652:Mettl3	UTSW	14	52,532,549 (GRCm39)	missense	probably damaging	1.00
R5462:Mettl3	UTSW	14	52,537,336 (GRCm39)	missense	probably damaging	0.96
R6046:Mettl3	UTSW	14	52,536,243 (GRCm39)	missense	possibly damaging	0.91
R6151:Mettl3	UTSW	14	52,532,477 (GRCm39)	missense	probably damaging	1.00
R6169:Mettl3	UTSW	14	52,536,214 (GRCm39)	missense	possibly damaging	0.88
R6225:Mettl3	UTSW	14	52,534,215 (GRCm39)	splice site	probably null
R6282:Mettl3	UTSW	14	52,535,428 (GRCm39)	missense	probably benign	0.01
R8038:Mettl3	UTSW	14	52,537,421 (GRCm39)	missense	possibly damaging	0.80
R8110:Mettl3	UTSW	14	52,537,709 (GRCm39)	missense	probably benign	0.02
R9332:Mettl3	UTSW	14	52,534,125 (GRCm39)	missense	probably damaging	1.00
R9757:Mettl3	UTSW	14	52,537,361 (GRCm39)	missense	probably benign	0.00
RF001:Mettl3	UTSW	14	52,537,756 (GRCm39)	missense	probably benign
X0025:Mettl3	UTSW	14	52,535,545 (GRCm39)	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- CTCTCTATTGAGTGCCAACAGC -3'
(R):5'- AAGCTGTCTGCCATGATGGG -3'

Sequencing Primer
(F):5'- CTCTACCATACTGTGATAACACTGG -3'
(R):5'- TAGCAGGGACCATCGCAG -3'

Posted On

2016-04-27