Incidental Mutation 'R4967:Sspo'
ID 384102
Institutional Source Beutler Lab
Gene Symbol Sspo
Ensembl Gene ENSMUSG00000029797
Gene Name SCO-spondin
Synonyms C79529, Scospondin
MMRRC Submission 042563-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R4967 (G1)
Quality Score 119
Status Validated
Chromosome 6
Chromosomal Location 48425163-48478184 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 48441539 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Leucine to Proline at position 1892 (L1892P)
Ref Sequence ENSEMBL: ENSMUSP00000131401 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000043676] [ENSMUST00000169350] [ENSMUST00000212740]
AlphaFold no structure available at present
Predicted Effect possibly damaging
Transcript: ENSMUST00000043676
AA Change: L1767P

PolyPhen 2 Score 0.741 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000047991
Gene: ENSMUSG00000029797
AA Change: L1767P

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
low complexity region 126 135 N/A INTRINSIC
Pfam:VWD 154 219 7.4e-11 PFAM
C8 267 346 2.3e-10 SMART
Pfam:TIL 349 404 3.2e-13 PFAM
VWC 406 448 2e-1 SMART
VWD 433 593 5.08e-29 SMART
C8 631 703 2.14e-28 SMART
Pfam:TIL 706 759 5.8e-11 PFAM
VWC 856 924 4.76e-2 SMART
VWD 883 1042 9.59e-48 SMART
C8 1076 1150 3.62e-26 SMART
Pfam:TIL 1153 1209 2.6e-13 PFAM
LDLa 1253 1291 2.29e-13 SMART
LDLa 1293 1328 1.87e-9 SMART
LDLa 1329 1366 5.77e-10 SMART
LDLa 1369 1408 1.52e-9 SMART
LDLa 1442 1479 2.55e-11 SMART
LDLa 1480 1520 5.6e-8 SMART
LDLa 1533 1574 2.29e-4 SMART
TSP1 1575 1626 6.47e-13 SMART
TSP1 1631 1686 1.35e-10 SMART
Pfam:TIL 1690 1746 3.1e-9 PFAM
TSP1 1774 1827 6.94e-2 SMART
VWC 1829 1886 4.95e-9 SMART
low complexity region 1901 1911 N/A INTRINSIC
FA58C 1928 2085 1.4e-2 SMART
LDLa 2091 2128 1.48e-7 SMART
LDLa 2242 2279 5.68e-9 SMART
LDLa 2299 2336 5.77e-10 SMART
TSP1 2339 2389 1.42e-9 SMART
TSP1 2394 2446 6.36e-21 SMART
Pfam:TIL 2460 2511 5.7e-10 PFAM
VWC 2513 2567 2.48e-1 SMART
TSP1 2554 2605 3.07e-14 SMART
TSP1 2611 2664 4.05e-5 SMART
TSP1 2669 2719 1.83e-12 SMART
EGF_like 2733 2776 5.45e1 SMART
VWC 2783 2836 2.73e-11 SMART
TSP1 2823 2875 3.72e-13 SMART
TSP1 2878 2919 6.05e-4 SMART
Pfam:TIL 2926 2978 1.1e-11 PFAM
VWC 2980 3035 9.77e-2 SMART
TSP1 3022 3086 6.68e-6 SMART
TSP1 3091 3143 1.08e-14 SMART
Pfam:TIL 3147 3201 2.2e-9 PFAM
VWC 3203 3260 2.72e-1 SMART
TSP1 3247 3306 3.72e-4 SMART
TSP1 3311 3363 5.27e-4 SMART
Pfam:TIL 3365 3421 4.2e-9 PFAM
TSP1 3484 3529 1.87e-9 SMART
low complexity region 3591 3601 N/A INTRINSIC
TSP1 3660 3713 5.02e-10 SMART
TSP1 3730 3779 2.95e-7 SMART
TSP1 3796 3849 1.99e-13 SMART
TSP1 3854 3906 2.51e-10 SMART
Pfam:TIL 3909 3964 3.4e-11 PFAM
VWC 3966 4022 1.26e0 SMART
TSP1 4009 4059 4.05e-5 SMART
TSP1 4103 4155 3.19e-12 SMART
TSP1 4161 4213 2.87e-2 SMART
TSP1 4218 4269 1.45e-6 SMART
Pfam:TIL 4273 4328 2.1e-10 PFAM
TSP1 4468 4516 7.56e-5 SMART
low complexity region 4551 4562 N/A INTRINSIC
VWC 4578 4652 5.21e-1 SMART
TSP1 4619 4669 3.92e-12 SMART
Pfam:TIL 4671 4725 1.5e-11 PFAM
Pfam:TIL 4777 4835 3.1e-9 PFAM
VWC 4837 4892 1.8e-11 SMART
GHB 4904 4997 1.02e0 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000169350
AA Change: L1892P

PolyPhen 2 Score 0.968 (Sensitivity: 0.77; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000131401
Gene: ENSMUSG00000029797
AA Change: L1892P

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
low complexity region 126 135 N/A INTRINSIC
VWD 185 341 4.36e-28 SMART
C8 390 469 2.3e-10 SMART
Pfam:TIL 472 527 8.6e-13 PFAM
VWC 529 571 2e-1 SMART
VWD 556 716 5.08e-29 SMART
C8 754 826 2.14e-28 SMART
Pfam:TIL 829 882 1.6e-10 PFAM
VWC 979 1047 4.76e-2 SMART
VWD 1006 1165 9.59e-48 SMART
C8 1201 1275 3.62e-26 SMART
Pfam:TIL 1278 1334 7e-13 PFAM
LDLa 1378 1416 2.29e-13 SMART
LDLa 1418 1453 1.87e-9 SMART
LDLa 1454 1491 5.77e-10 SMART
LDLa 1494 1533 1.52e-9 SMART
LDLa 1567 1604 2.55e-11 SMART
LDLa 1605 1645 5.6e-8 SMART
LDLa 1658 1699 2.29e-4 SMART
TSP1 1700 1751 6.47e-13 SMART
TSP1 1756 1811 1.35e-10 SMART
Pfam:TIL 1815 1871 8.3e-9 PFAM
VWC 1873 1928 2.42e-1 SMART
TSP1 1915 1968 6.94e-2 SMART
VWC 1970 2027 4.95e-9 SMART
low complexity region 2042 2052 N/A INTRINSIC
FA58C 2069 2226 1.4e-2 SMART
LDLa 2232 2269 1.48e-7 SMART
LDLa 2387 2424 5.68e-9 SMART
LDLa 2444 2481 5.77e-10 SMART
TSP1 2484 2534 1.42e-9 SMART
TSP1 2539 2591 6.36e-21 SMART
Pfam:TIL 2606 2656 1.8e-9 PFAM
VWC 2658 2712 2.48e-1 SMART
TSP1 2699 2750 3.07e-14 SMART
TSP1 2756 2809 4.05e-5 SMART
TSP1 2814 2864 1.83e-12 SMART
EGF_like 2878 2921 5.45e1 SMART
VWC 2928 2981 2.73e-11 SMART
TSP1 2968 3020 3.72e-13 SMART
TSP1 3023 3064 6.05e-4 SMART
Pfam:TIL 3071 3123 3e-11 PFAM
VWC 3125 3180 9.77e-2 SMART
TSP1 3167 3231 6.68e-6 SMART
TSP1 3236 3288 1.08e-14 SMART
Pfam:TIL 3292 3346 6e-9 PFAM
VWC 3348 3405 2.72e-1 SMART
TSP1 3392 3451 3.72e-4 SMART
TSP1 3456 3508 5.27e-4 SMART
Pfam:TIL 3510 3566 1.1e-8 PFAM
TSP1 3629 3674 1.87e-9 SMART
low complexity region 3734 3744 N/A INTRINSIC
TSP1 3803 3856 5.02e-10 SMART
TSP1 3873 3922 2.95e-7 SMART
TSP1 3939 3992 1.99e-13 SMART
TSP1 3997 4049 2.51e-10 SMART
Pfam:TIL 4052 4107 9.1e-11 PFAM
VWC 4109 4165 1.26e0 SMART
TSP1 4152 4202 4.05e-5 SMART
TSP1 4246 4298 3.19e-12 SMART
TSP1 4304 4356 2.87e-2 SMART
TSP1 4361 4412 1.45e-6 SMART
Pfam:TIL 4416 4471 5.6e-10 PFAM
TSP1 4611 4659 7.56e-5 SMART
low complexity region 4694 4705 N/A INTRINSIC
VWC 4721 4795 5.21e-1 SMART
TSP1 4762 4812 3.92e-12 SMART
Pfam:TIL 4814 4868 4e-11 PFAM
Pfam:TIL 4920 4978 8.4e-9 PFAM
VWC 4980 5035 1.8e-11 SMART
GHB 5050 5143 1.02e0 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000212740
AA Change: L1892P

PolyPhen 2 Score 0.703 (Sensitivity: 0.86; Specificity: 0.92)
Meta Mutation Damage Score 0.1489 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.7%
Validation Efficiency 100% (102/102)
Allele List at MGI
Other mutations in this stock
Total: 93 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930447C04Rik A T 12: 72,956,502 (GRCm39) Y199* probably null Het
Adhfe1 A T 1: 9,637,029 (GRCm39) I394F probably benign Het
Arhgap26 G T 18: 39,379,893 (GRCm39) R485L probably damaging Het
Atat1 T A 17: 36,212,467 (GRCm39) N231I probably damaging Het
B4galnt2 T C 11: 95,760,100 (GRCm39) N309S probably benign Het
Bank1 A T 3: 135,772,134 (GRCm39) F499I probably damaging Het
Bcl9l A G 9: 44,416,365 (GRCm39) D146G possibly damaging Het
Bglap3 A T 3: 88,283,671 (GRCm39) probably benign Het
Bscl2 C A 19: 8,825,344 (GRCm39) T376K probably benign Het
Cercam A T 2: 29,761,033 (GRCm39) probably null Het
Clec4f C T 6: 83,633,012 (GRCm39) M1I probably null Het
Clec4n A T 6: 123,209,066 (GRCm39) I14F probably benign Het
Cmya5 T C 13: 93,227,093 (GRCm39) E2665G probably damaging Het
Cog4 T A 8: 111,578,915 (GRCm39) probably null Het
Cubn A G 2: 13,352,856 (GRCm39) F1961L probably benign Het
Cyp4a29 A T 4: 115,104,196 (GRCm39) H88L probably benign Het
Dhdh A G 7: 45,128,530 (GRCm39) L216P probably damaging Het
Dpp6 T C 5: 27,871,509 (GRCm39) F544S probably damaging Het
Dthd1 C T 5: 63,045,549 (GRCm39) T771I probably benign Het
Emc10 A G 7: 44,142,612 (GRCm39) probably null Het
Fgg A T 3: 82,920,072 (GRCm39) T284S probably benign Het
Gask1a C T 9: 121,794,784 (GRCm39) R313W probably damaging Het
Gm3002 T A 14: 3,824,737 (GRCm38) N24K probably damaging Het
Gm8674 G A 13: 50,056,034 (GRCm39) noncoding transcript Het
Gpr63 G A 4: 25,008,368 (GRCm39) W364* probably null Het
Hcn4 C G 9: 58,767,111 (GRCm39) P891A unknown Het
Hcrtr1 A T 4: 130,024,792 (GRCm39) F365I possibly damaging Het
Hmcn2 A G 2: 31,244,176 (GRCm39) probably null Het
Hoxc5 T A 15: 102,923,786 (GRCm39) L194H probably damaging Het
Ifna11 A G 4: 88,738,287 (GRCm39) N31S probably null Het
Ikbke GCC G 1: 131,203,004 (GRCm39) probably null Het
Iqgap2 A T 13: 95,766,514 (GRCm39) D1496E probably benign Het
Kif28 G T 1: 179,536,007 (GRCm39) Q556K probably damaging Het
Klhl42 C T 6: 147,009,502 (GRCm39) T447I possibly damaging Het
Lrp1b A C 2: 41,678,986 (GRCm39) D35E probably damaging Het
Lsm14b A G 2: 179,675,692 (GRCm39) probably benign Het
Map3k1 T C 13: 111,909,272 (GRCm39) E226G probably damaging Het
Map3k14 T A 11: 103,130,357 (GRCm39) N187Y probably benign Het
Mcm6 A T 1: 128,263,586 (GRCm39) V645E probably damaging Het
Mdh1b G T 1: 63,759,022 (GRCm39) P190Q probably damaging Het
Meiob T G 17: 25,037,353 (GRCm39) L77R probably damaging Het
Mrgpra3 A T 7: 47,239,267 (GRCm39) F220I probably benign Het
Mrtfa C A 15: 80,929,476 (GRCm39) probably benign Het
Mtor T A 4: 148,575,817 (GRCm39) S1324T possibly damaging Het
Myot T A 18: 44,487,995 (GRCm39) D437E possibly damaging Het
Ncoa6 G T 2: 155,263,252 (GRCm39) T394K possibly damaging Het
Nf1 T A 11: 79,456,379 (GRCm39) probably null Het
Nup210 T A 6: 91,013,451 (GRCm39) T1190S possibly damaging Het
Odf1 T A 15: 38,226,652 (GRCm39) I184N probably damaging Het
Or10d1 C T 9: 39,484,054 (GRCm39) C167Y probably damaging Het
Or13d1 T C 4: 52,970,960 (GRCm39) V113A possibly damaging Het
Or1n1b A G 2: 36,780,719 (GRCm39) I47T probably damaging Het
Or5k15 G T 16: 58,709,957 (GRCm39) Q209K possibly damaging Het
Padi1 A G 4: 140,572,901 (GRCm39) V21A probably benign Het
Pdzrn3 C T 6: 101,128,551 (GRCm39) R705H probably damaging Het
Pik3cb T C 9: 98,987,685 (GRCm39) I18V probably benign Het
Pmpca T C 2: 26,280,320 (GRCm39) S117P probably damaging Het
Poteg T A 8: 27,985,009 (GRCm39) probably benign Het
Pramel32 T C 4: 88,547,432 (GRCm39) T80A probably damaging Het
Rab30 G A 7: 92,478,771 (GRCm39) R72H probably damaging Het
Ramp2 T A 11: 101,138,383 (GRCm39) probably null Het
Rbks G A 5: 31,781,876 (GRCm39) T308I probably damaging Het
Rnf112 A G 11: 61,343,752 (GRCm39) probably benign Het
Rnf38 G A 4: 44,152,460 (GRCm39) P3S probably damaging Het
Sec16a A G 2: 26,302,883 (GRCm39) S2344P probably benign Het
Slc16a8 C T 15: 79,137,084 (GRCm39) V109M possibly damaging Het
Slc28a1 A T 7: 80,791,757 (GRCm39) T308S possibly damaging Het
Slc39a3 T C 10: 80,867,453 (GRCm39) T98A possibly damaging Het
Smarcc2 T C 10: 128,319,049 (GRCm39) F731L probably damaging Het
Smc4 A G 3: 68,925,572 (GRCm39) probably benign Het
Sparcl1 T G 5: 104,240,776 (GRCm39) D216A probably damaging Het
Speg G A 1: 75,364,513 (GRCm39) R192H probably damaging Het
Styxl2 A T 1: 165,954,675 (GRCm39) V25E probably damaging Het
Tacc2 A G 7: 130,225,678 (GRCm39) N807D probably damaging Het
Teddm1a A T 1: 153,767,979 (GRCm39) K148* probably null Het
Tex15 T A 8: 34,064,498 (GRCm39) D1309E probably benign Het
Thbs1 A T 2: 117,945,259 (GRCm39) E277D probably benign Het
Ticrr G A 7: 79,310,158 (GRCm39) R24Q probably damaging Het
Tigd4 A G 3: 84,502,460 (GRCm39) E459G probably benign Het
Tln2 C A 9: 67,262,407 (GRCm39) A615S probably damaging Het
Tmprss5 T C 9: 49,026,817 (GRCm39) V410A probably damaging Het
Tnrc6a G T 7: 122,789,095 (GRCm39) W1638L probably damaging Het
Tpr A G 1: 150,285,810 (GRCm39) D424G probably damaging Het
Trim34a A T 7: 103,910,271 (GRCm39) K358* probably null Het
Tssk5 T A 15: 76,258,856 (GRCm39) D10V possibly damaging Het
Usp35 A C 7: 96,962,782 (GRCm39) L470R probably damaging Het
Usp42 T C 5: 143,701,119 (GRCm39) D968G possibly damaging Het
Utrn A G 10: 12,331,164 (GRCm39) V2924A probably damaging Het
Wdtc1 C A 4: 133,021,654 (GRCm39) A627S probably damaging Het
Xrra1 A G 7: 99,555,730 (GRCm39) T366A probably damaging Het
Zfp712 T A 13: 67,188,773 (GRCm39) K585* probably null Het
Zfp97 T A 17: 17,365,393 (GRCm39) N297K probably damaging Het
Zfp97 G T 17: 17,364,938 (GRCm39) E146* probably null Het
Other mutations in Sspo
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00333:Sspo APN 6 48,447,387 (GRCm39) missense probably benign 0.02
IGL00339:Sspo APN 6 48,460,680 (GRCm39) splice site probably benign
IGL00391:Sspo APN 6 48,474,320 (GRCm39) missense probably damaging 0.96
IGL00433:Sspo APN 6 48,466,970 (GRCm39) missense probably damaging 1.00
IGL00471:Sspo APN 6 48,475,147 (GRCm39) splice site probably benign
IGL00500:Sspo APN 6 48,474,355 (GRCm39) nonsense probably null
IGL00537:Sspo APN 6 48,475,147 (GRCm39) splice site probably benign
IGL00540:Sspo APN 6 48,475,147 (GRCm39) splice site probably benign
IGL01060:Sspo APN 6 48,426,413 (GRCm39) nonsense probably null
IGL01090:Sspo APN 6 48,467,059 (GRCm39) missense probably benign 0.08
IGL01125:Sspo APN 6 48,469,822 (GRCm39) missense probably damaging 1.00
IGL01447:Sspo APN 6 48,441,600 (GRCm39) splice site probably null
IGL01457:Sspo APN 6 48,475,277 (GRCm39) missense probably benign 0.00
IGL01481:Sspo APN 6 48,425,449 (GRCm39) missense probably benign 0.41
IGL01485:Sspo APN 6 48,455,665 (GRCm39) missense probably damaging 1.00
IGL01544:Sspo APN 6 48,467,953 (GRCm39) missense probably damaging 0.99
IGL01575:Sspo APN 6 48,435,976 (GRCm39) missense probably benign 0.01
IGL01589:Sspo APN 6 48,428,112 (GRCm39) missense probably damaging 1.00
IGL01601:Sspo APN 6 48,463,313 (GRCm39) missense probably benign 0.33
IGL01644:Sspo APN 6 48,429,436 (GRCm39) missense probably benign
IGL01659:Sspo APN 6 48,451,377 (GRCm39) missense probably damaging 1.00
IGL01801:Sspo APN 6 48,434,072 (GRCm39) missense probably damaging 1.00
IGL01872:Sspo APN 6 48,431,623 (GRCm39) missense probably damaging 0.99
IGL01874:Sspo APN 6 48,429,124 (GRCm39) missense probably damaging 1.00
IGL01936:Sspo APN 6 48,452,821 (GRCm39) missense probably damaging 1.00
IGL01941:Sspo APN 6 48,472,116 (GRCm39) missense probably benign 0.19
IGL01986:Sspo APN 6 48,460,237 (GRCm39) missense probably benign 0.05
IGL01987:Sspo APN 6 48,454,558 (GRCm39) splice site probably null
IGL02170:Sspo APN 6 48,444,917 (GRCm39) missense possibly damaging 0.76
IGL02192:Sspo APN 6 48,436,502 (GRCm39) missense possibly damaging 0.86
IGL02210:Sspo APN 6 48,477,426 (GRCm39) missense probably damaging 1.00
IGL02225:Sspo APN 6 48,461,268 (GRCm39) missense probably benign 0.09
IGL02280:Sspo APN 6 48,473,165 (GRCm39) missense probably damaging 1.00
IGL02303:Sspo APN 6 48,461,639 (GRCm39) missense possibly damaging 0.52
IGL02397:Sspo APN 6 48,438,572 (GRCm39) missense probably benign 0.35
IGL02451:Sspo APN 6 48,437,237 (GRCm39) splice site probably benign
IGL02500:Sspo APN 6 48,455,313 (GRCm39) nonsense probably null
IGL02519:Sspo APN 6 48,461,762 (GRCm39) missense probably damaging 1.00
IGL02549:Sspo APN 6 48,428,707 (GRCm39) missense possibly damaging 0.81
IGL02562:Sspo APN 6 48,467,056 (GRCm39) splice site probably null
IGL02673:Sspo APN 6 48,475,709 (GRCm39) critical splice donor site probably null
IGL02673:Sspo APN 6 48,452,794 (GRCm39) missense probably damaging 1.00
IGL02719:Sspo APN 6 48,459,601 (GRCm39) missense probably benign 0.39
IGL02793:Sspo APN 6 48,464,828 (GRCm39) splice site probably benign
IGL03003:Sspo APN 6 48,432,021 (GRCm39) missense probably damaging 0.98
IGL03056:Sspo APN 6 48,447,472 (GRCm39) missense probably benign 0.17
IGL03105:Sspo APN 6 48,450,592 (GRCm39) splice site probably benign
IGL03116:Sspo APN 6 48,471,035 (GRCm39) missense probably benign 0.32
IGL03163:Sspo APN 6 48,461,266 (GRCm39) missense probably benign 0.19
IGL03198:Sspo APN 6 48,454,516 (GRCm39) missense probably benign 0.31
IGL03365:Sspo APN 6 48,436,349 (GRCm39) missense possibly damaging 0.82
Barrier UTSW 6 48,472,146 (GRCm39) missense possibly damaging 0.58
R0312_sspo_280 UTSW 6 48,432,335 (GRCm39) missense possibly damaging 0.52
R3112_Sspo_731 UTSW 6 48,434,534 (GRCm39) missense probably damaging 1.00
R3498_Sspo_650 UTSW 6 48,444,914 (GRCm39) missense possibly damaging 0.58
R4180_Sspo_324 UTSW 6 48,475,329 (GRCm39) critical splice donor site probably null
spotsylvania UTSW 6 48,453,505 (GRCm39) nonsense probably null
ANU74:Sspo UTSW 6 48,437,893 (GRCm39) missense probably damaging 1.00
IGL02984:Sspo UTSW 6 48,472,089 (GRCm39) missense probably benign 0.33
IGL03052:Sspo UTSW 6 48,437,387 (GRCm39) missense probably damaging 1.00
IGL03134:Sspo UTSW 6 48,427,999 (GRCm39) missense probably benign 0.28
PIT4531001:Sspo UTSW 6 48,458,173 (GRCm39) missense probably benign
R0087:Sspo UTSW 6 48,454,719 (GRCm39) missense probably damaging 1.00
R0122:Sspo UTSW 6 48,450,910 (GRCm39) missense possibly damaging 0.95
R0129:Sspo UTSW 6 48,432,352 (GRCm39) missense probably benign 0.00
R0164:Sspo UTSW 6 48,471,128 (GRCm39) splice site probably benign
R0195:Sspo UTSW 6 48,463,570 (GRCm39) missense probably benign
R0200:Sspo UTSW 6 48,463,349 (GRCm39) missense probably null 0.01
R0201:Sspo UTSW 6 48,432,686 (GRCm39) missense possibly damaging 0.64
R0241:Sspo UTSW 6 48,438,429 (GRCm39) missense possibly damaging 0.82
R0241:Sspo UTSW 6 48,438,429 (GRCm39) missense possibly damaging 0.82
R0243:Sspo UTSW 6 48,470,120 (GRCm39) missense probably damaging 1.00
R0268:Sspo UTSW 6 48,442,489 (GRCm39) missense probably benign 0.26
R0312:Sspo UTSW 6 48,432,335 (GRCm39) missense possibly damaging 0.52
R0449:Sspo UTSW 6 48,443,674 (GRCm39) missense probably damaging 1.00
R0523:Sspo UTSW 6 48,428,794 (GRCm39) missense probably benign 0.20
R0576:Sspo UTSW 6 48,441,876 (GRCm39) splice site probably null
R0671:Sspo UTSW 6 48,467,325 (GRCm39) splice site probably benign
R0828:Sspo UTSW 6 48,475,668 (GRCm39) missense probably damaging 1.00
R0880:Sspo UTSW 6 48,452,869 (GRCm39) missense possibly damaging 0.69
R0903:Sspo UTSW 6 48,432,242 (GRCm39) critical splice acceptor site probably null
R1051:Sspo UTSW 6 48,468,389 (GRCm39) nonsense probably null
R1083:Sspo UTSW 6 48,447,933 (GRCm39) missense possibly damaging 0.91
R1109:Sspo UTSW 6 48,474,377 (GRCm39) missense probably damaging 1.00
R1118:Sspo UTSW 6 48,436,352 (GRCm39) missense probably damaging 0.97
R1256:Sspo UTSW 6 48,434,573 (GRCm39) missense probably damaging 1.00
R1342:Sspo UTSW 6 48,438,569 (GRCm39) missense probably benign 0.07
R1355:Sspo UTSW 6 48,425,560 (GRCm39) missense probably benign 0.41
R1370:Sspo UTSW 6 48,425,560 (GRCm39) missense probably benign 0.41
R1469:Sspo UTSW 6 48,467,916 (GRCm39) missense probably damaging 1.00
R1469:Sspo UTSW 6 48,467,916 (GRCm39) missense probably damaging 1.00
R1476:Sspo UTSW 6 48,440,334 (GRCm39) critical splice donor site probably null
R1566:Sspo UTSW 6 48,443,804 (GRCm39) critical splice donor site probably null
R1630:Sspo UTSW 6 48,434,658 (GRCm39) missense probably benign 0.01
R1686:Sspo UTSW 6 48,437,334 (GRCm39) missense probably benign 0.00
R1707:Sspo UTSW 6 48,454,811 (GRCm39) missense probably damaging 0.99
R1727:Sspo UTSW 6 48,471,782 (GRCm39) missense probably damaging 1.00
R1822:Sspo UTSW 6 48,469,820 (GRCm39) missense possibly damaging 0.75
R1831:Sspo UTSW 6 48,466,720 (GRCm39) missense probably damaging 1.00
R1835:Sspo UTSW 6 48,434,274 (GRCm39) missense probably damaging 0.97
R1862:Sspo UTSW 6 48,467,940 (GRCm39) missense probably damaging 0.98
R1878:Sspo UTSW 6 48,436,300 (GRCm39) missense possibly damaging 0.92
R1900:Sspo UTSW 6 48,436,284 (GRCm39) missense probably benign 0.22
R1945:Sspo UTSW 6 48,466,707 (GRCm39) missense possibly damaging 0.93
R1957:Sspo UTSW 6 48,455,207 (GRCm39) missense probably damaging 0.99
R1990:Sspo UTSW 6 48,427,984 (GRCm39) missense probably benign 0.00
R1996:Sspo UTSW 6 48,452,424 (GRCm39) missense possibly damaging 0.50
R2049:Sspo UTSW 6 48,440,465 (GRCm39) missense probably benign 0.36
R2049:Sspo UTSW 6 48,437,697 (GRCm39) splice site probably benign
R2064:Sspo UTSW 6 48,450,596 (GRCm39) missense probably damaging 0.99
R2072:Sspo UTSW 6 48,450,451 (GRCm39) missense probably benign 0.01
R2096:Sspo UTSW 6 48,438,608 (GRCm39) missense probably benign
R2106:Sspo UTSW 6 48,443,250 (GRCm39) missense possibly damaging 0.96
R2230:Sspo UTSW 6 48,477,437 (GRCm39) missense probably benign 0.11
R2230:Sspo UTSW 6 48,425,606 (GRCm39) missense probably damaging 0.97
R2232:Sspo UTSW 6 48,425,606 (GRCm39) missense probably damaging 0.97
R2351:Sspo UTSW 6 48,441,803 (GRCm39) missense probably damaging 1.00
R2423:Sspo UTSW 6 48,430,989 (GRCm39) missense probably benign 0.00
R2508:Sspo UTSW 6 48,441,298 (GRCm39) missense probably damaging 1.00
R3110:Sspo UTSW 6 48,434,534 (GRCm39) missense probably damaging 1.00
R3112:Sspo UTSW 6 48,434,534 (GRCm39) missense probably damaging 1.00
R3413:Sspo UTSW 6 48,457,631 (GRCm39) missense probably damaging 1.00
R3433:Sspo UTSW 6 48,452,885 (GRCm39) splice site probably null
R3498:Sspo UTSW 6 48,444,914 (GRCm39) missense possibly damaging 0.58
R3732:Sspo UTSW 6 48,426,864 (GRCm39) missense probably damaging 1.00
R3816:Sspo UTSW 6 48,458,037 (GRCm39) missense possibly damaging 0.77
R3818:Sspo UTSW 6 48,458,037 (GRCm39) missense possibly damaging 0.77
R3819:Sspo UTSW 6 48,458,037 (GRCm39) missense possibly damaging 0.77
R3838:Sspo UTSW 6 48,457,754 (GRCm39) missense probably damaging 1.00
R3850:Sspo UTSW 6 48,469,424 (GRCm39) missense probably damaging 1.00
R3880:Sspo UTSW 6 48,471,874 (GRCm39) missense probably benign 0.38
R3893:Sspo UTSW 6 48,453,505 (GRCm39) nonsense probably null
R4116:Sspo UTSW 6 48,433,928 (GRCm39) missense probably damaging 0.99
R4179:Sspo UTSW 6 48,475,329 (GRCm39) critical splice donor site probably null
R4180:Sspo UTSW 6 48,475,329 (GRCm39) critical splice donor site probably null
R4207:Sspo UTSW 6 48,455,227 (GRCm39) missense probably benign 0.00
R4210:Sspo UTSW 6 48,441,835 (GRCm39) missense probably benign 0.00
R4223:Sspo UTSW 6 48,428,091 (GRCm39) missense possibly damaging 0.54
R4224:Sspo UTSW 6 48,428,091 (GRCm39) missense possibly damaging 0.54
R4225:Sspo UTSW 6 48,428,091 (GRCm39) missense possibly damaging 0.54
R4229:Sspo UTSW 6 48,467,868 (GRCm39) missense probably benign 0.00
R4230:Sspo UTSW 6 48,467,868 (GRCm39) missense probably benign 0.00
R4363:Sspo UTSW 6 48,475,665 (GRCm39) missense probably damaging 1.00
R4370:Sspo UTSW 6 48,443,282 (GRCm39) missense probably null 0.14
R4407:Sspo UTSW 6 48,437,454 (GRCm39) missense probably damaging 1.00
R4438:Sspo UTSW 6 48,464,287 (GRCm39) missense probably damaging 1.00
R4454:Sspo UTSW 6 48,464,159 (GRCm39) missense probably benign 0.05
R4455:Sspo UTSW 6 48,442,450 (GRCm39) missense probably damaging 1.00
R4561:Sspo UTSW 6 48,452,468 (GRCm39) splice site probably null
R4574:Sspo UTSW 6 48,442,457 (GRCm39) missense probably damaging 1.00
R4578:Sspo UTSW 6 48,440,307 (GRCm39) missense possibly damaging 0.58
R4653:Sspo UTSW 6 48,455,580 (GRCm39) missense probably damaging 1.00
R4656:Sspo UTSW 6 48,431,010 (GRCm39) missense possibly damaging 0.65
R4659:Sspo UTSW 6 48,461,147 (GRCm39) missense probably damaging 1.00
R4664:Sspo UTSW 6 48,450,468 (GRCm39) missense possibly damaging 0.82
R4685:Sspo UTSW 6 48,469,828 (GRCm39) missense probably damaging 0.98
R4692:Sspo UTSW 6 48,459,621 (GRCm39) missense probably damaging 1.00
R4703:Sspo UTSW 6 48,477,387 (GRCm39) missense probably damaging 1.00
R4704:Sspo UTSW 6 48,475,638 (GRCm39) missense probably damaging 1.00
R4738:Sspo UTSW 6 48,455,330 (GRCm39) missense possibly damaging 0.78
R4766:Sspo UTSW 6 48,447,514 (GRCm39) missense probably benign 0.04
R4771:Sspo UTSW 6 48,437,813 (GRCm39) missense probably damaging 1.00
R4790:Sspo UTSW 6 48,437,705 (GRCm39) missense probably benign 0.04
R4792:Sspo UTSW 6 48,438,519 (GRCm39) missense probably benign 0.00
R4808:Sspo UTSW 6 48,428,095 (GRCm39) missense probably damaging 1.00
R4812:Sspo UTSW 6 48,467,444 (GRCm39) missense probably benign 0.00
R4883:Sspo UTSW 6 48,437,756 (GRCm39) missense probably benign 0.00
R4906:Sspo UTSW 6 48,442,664 (GRCm39) critical splice acceptor site probably null
R4934:Sspo UTSW 6 48,442,486 (GRCm39) missense probably damaging 1.00
R4945:Sspo UTSW 6 48,444,021 (GRCm39) splice site probably null
R5016:Sspo UTSW 6 48,429,214 (GRCm39) nonsense probably null
R5018:Sspo UTSW 6 48,432,634 (GRCm39) missense probably damaging 1.00
R5034:Sspo UTSW 6 48,457,757 (GRCm39) missense possibly damaging 0.93
R5044:Sspo UTSW 6 48,443,889 (GRCm39) critical splice acceptor site probably null
R5055:Sspo UTSW 6 48,441,729 (GRCm39) missense probably damaging 1.00
R5087:Sspo UTSW 6 48,465,405 (GRCm39) missense possibly damaging 0.51
R5155:Sspo UTSW 6 48,437,408 (GRCm39) missense probably benign 0.03
R5223:Sspo UTSW 6 48,455,258 (GRCm39) missense probably damaging 1.00
R5249:Sspo UTSW 6 48,470,244 (GRCm39) missense probably damaging 0.98
R5257:Sspo UTSW 6 48,453,428 (GRCm39) missense probably damaging 1.00
R5258:Sspo UTSW 6 48,453,428 (GRCm39) missense probably damaging 1.00
R5276:Sspo UTSW 6 48,467,401 (GRCm39) missense probably damaging 1.00
R5307:Sspo UTSW 6 48,431,784 (GRCm39) missense probably damaging 0.99
R5341:Sspo UTSW 6 48,436,549 (GRCm39) missense probably damaging 1.00
R5361:Sspo UTSW 6 48,443,247 (GRCm39) missense probably benign 0.02
R5385:Sspo UTSW 6 48,439,187 (GRCm39) missense probably benign 0.18
R5394:Sspo UTSW 6 48,472,194 (GRCm39) missense possibly damaging 0.52
R5477:Sspo UTSW 6 48,475,327 (GRCm39) missense possibly damaging 0.60
R5490:Sspo UTSW 6 48,470,214 (GRCm39) missense probably benign 0.33
R5512:Sspo UTSW 6 48,432,605 (GRCm39) missense probably damaging 0.97
R5518:Sspo UTSW 6 48,473,588 (GRCm39) missense possibly damaging 0.92
R5530:Sspo UTSW 6 48,442,517 (GRCm39) missense probably damaging 0.97
R5538:Sspo UTSW 6 48,429,112 (GRCm39) missense probably damaging 0.99
R5590:Sspo UTSW 6 48,451,425 (GRCm39) missense probably damaging 1.00
R5613:Sspo UTSW 6 48,431,978 (GRCm39) missense possibly damaging 0.79
R5638:Sspo UTSW 6 48,469,825 (GRCm39) missense possibly damaging 0.86
R5809:Sspo UTSW 6 48,436,979 (GRCm39) missense possibly damaging 0.59
R5810:Sspo UTSW 6 48,460,832 (GRCm39) missense probably benign 0.02
R5814:Sspo UTSW 6 48,428,818 (GRCm39) missense probably damaging 1.00
R5915:Sspo UTSW 6 48,468,418 (GRCm39) missense possibly damaging 0.83
R5915:Sspo UTSW 6 48,441,530 (GRCm39) missense probably benign 0.00
R5979:Sspo UTSW 6 48,440,627 (GRCm39) missense probably benign 0.20
R5996:Sspo UTSW 6 48,471,110 (GRCm39) missense possibly damaging 0.87
R6012:Sspo UTSW 6 48,428,305 (GRCm39) missense probably benign 0.00
R6025:Sspo UTSW 6 48,463,720 (GRCm39) missense possibly damaging 0.83
R6120:Sspo UTSW 6 48,442,510 (GRCm39) missense probably damaging 1.00
R6150:Sspo UTSW 6 48,463,313 (GRCm39) missense probably benign 0.33
R6221:Sspo UTSW 6 48,440,639 (GRCm39) missense probably damaging 1.00
R6261:Sspo UTSW 6 48,439,125 (GRCm39) missense possibly damaging 0.75
R6312:Sspo UTSW 6 48,434,300 (GRCm39) critical splice donor site probably null
R6372:Sspo UTSW 6 48,449,475 (GRCm39) missense probably damaging 1.00
R6456:Sspo UTSW 6 48,428,740 (GRCm39) missense probably benign 0.08
R6497:Sspo UTSW 6 48,472,142 (GRCm39) missense possibly damaging 0.71
R6501:Sspo UTSW 6 48,472,146 (GRCm39) missense possibly damaging 0.58
R6617:Sspo UTSW 6 48,467,980 (GRCm39) missense possibly damaging 0.93
R6825:Sspo UTSW 6 48,442,459 (GRCm39) missense probably benign 0.04
R6831:Sspo UTSW 6 48,461,767 (GRCm39) missense possibly damaging 0.68
R6861:Sspo UTSW 6 48,464,889 (GRCm39) missense probably benign 0.15
R6961:Sspo UTSW 6 48,440,811 (GRCm39) missense probably benign 0.05
R6967:Sspo UTSW 6 48,466,728 (GRCm39) missense probably benign 0.21
R7016:Sspo UTSW 6 48,426,098 (GRCm39) missense probably damaging 1.00
R7035:Sspo UTSW 6 48,426,147 (GRCm39) splice site probably null
R7058:Sspo UTSW 6 48,425,516 (GRCm39) missense probably damaging 1.00
R7072:Sspo UTSW 6 48,431,913 (GRCm39) missense probably damaging 1.00
R7078:Sspo UTSW 6 48,437,313 (GRCm39) missense probably damaging 1.00
R7082:Sspo UTSW 6 48,455,543 (GRCm39) critical splice acceptor site probably null
R7120:Sspo UTSW 6 48,442,505 (GRCm39) missense probably benign 0.05
R7127:Sspo UTSW 6 48,426,446 (GRCm39) missense probably benign 0.02
R7146:Sspo UTSW 6 48,478,029 (GRCm39) missense probably benign 0.15
R7220:Sspo UTSW 6 48,453,540 (GRCm39) nonsense probably null
R7242:Sspo UTSW 6 48,450,886 (GRCm39) missense probably benign
R7261:Sspo UTSW 6 48,427,011 (GRCm39) missense possibly damaging 0.52
R7313:Sspo UTSW 6 48,450,390 (GRCm39) missense probably benign 0.04
R7313:Sspo UTSW 6 48,431,762 (GRCm39) missense probably damaging 1.00
R7323:Sspo UTSW 6 48,438,581 (GRCm39) missense possibly damaging 0.93
R7330:Sspo UTSW 6 48,452,396 (GRCm39) missense probably benign 0.00
R7351:Sspo UTSW 6 48,441,855 (GRCm39) missense possibly damaging 0.89
R7467:Sspo UTSW 6 48,463,237 (GRCm39) missense probably damaging 1.00
R7475:Sspo UTSW 6 48,432,794 (GRCm39) missense probably benign 0.37
R7489:Sspo UTSW 6 48,450,647 (GRCm39) missense probably damaging 0.99
R7508:Sspo UTSW 6 48,443,633 (GRCm39) missense probably damaging 1.00
R7515:Sspo UTSW 6 48,470,820 (GRCm39) missense probably damaging 1.00
R7564:Sspo UTSW 6 48,426,434 (GRCm39) missense probably benign 0.04
R7607:Sspo UTSW 6 48,466,661 (GRCm39) missense probably damaging 1.00
R7620:Sspo UTSW 6 48,444,020 (GRCm39) critical splice donor site probably null
R7667:Sspo UTSW 6 48,452,305 (GRCm39) nonsense probably null
R7691:Sspo UTSW 6 48,461,163 (GRCm39) missense probably benign 0.12
R7707:Sspo UTSW 6 48,438,461 (GRCm39) missense probably benign 0.01
R7723:Sspo UTSW 6 48,441,572 (GRCm39) missense probably damaging 0.99
R7748:Sspo UTSW 6 48,426,399 (GRCm39) nonsense probably null
R7767:Sspo UTSW 6 48,428,316 (GRCm39) missense probably damaging 0.96
R7792:Sspo UTSW 6 48,431,624 (GRCm39) missense probably damaging 0.98
R7878:Sspo UTSW 6 48,469,460 (GRCm39) missense probably damaging 1.00
R7893:Sspo UTSW 6 48,440,244 (GRCm39) missense probably benign 0.02
R7942:Sspo UTSW 6 48,465,434 (GRCm39) splice site probably null
R7952:Sspo UTSW 6 48,464,263 (GRCm39) missense probably damaging 1.00
R7981:Sspo UTSW 6 48,445,428 (GRCm39) missense probably benign
R7995:Sspo UTSW 6 48,469,823 (GRCm39) missense probably damaging 1.00
R8088:Sspo UTSW 6 48,434,547 (GRCm39) missense probably damaging 1.00
R8129:Sspo UTSW 6 48,443,959 (GRCm39) missense possibly damaging 0.79
R8145:Sspo UTSW 6 48,444,683 (GRCm39) missense possibly damaging 0.49
R8202:Sspo UTSW 6 48,434,534 (GRCm39) missense probably damaging 1.00
R8211:Sspo UTSW 6 48,469,543 (GRCm39) critical splice donor site probably null
R8240:Sspo UTSW 6 48,460,436 (GRCm39) missense possibly damaging 0.84
R8252:Sspo UTSW 6 48,462,386 (GRCm39) missense probably damaging 0.99
R8270:Sspo UTSW 6 48,426,897 (GRCm39) missense probably benign
R8272:Sspo UTSW 6 48,425,453 (GRCm39) missense probably benign 0.03
R8316:Sspo UTSW 6 48,459,622 (GRCm39) missense probably damaging 1.00
R8384:Sspo UTSW 6 48,459,598 (GRCm39) missense probably damaging 1.00
R8390:Sspo UTSW 6 48,444,896 (GRCm39) missense probably benign 0.00
R8770:Sspo UTSW 6 48,451,206 (GRCm39) missense probably null 1.00
R8827:Sspo UTSW 6 48,434,606 (GRCm39) missense possibly damaging 0.59
R8882:Sspo UTSW 6 48,452,390 (GRCm39) missense probably damaging 1.00
R8886:Sspo UTSW 6 48,458,201 (GRCm39) missense possibly damaging 0.92
R8946:Sspo UTSW 6 48,434,071 (GRCm39) missense probably damaging 1.00
R8947:Sspo UTSW 6 48,425,504 (GRCm39) missense probably damaging 1.00
R9028:Sspo UTSW 6 48,473,087 (GRCm39) missense probably benign 0.38
R9043:Sspo UTSW 6 48,470,214 (GRCm39) missense probably benign 0.07
R9056:Sspo UTSW 6 48,450,608 (GRCm39) missense probably damaging 0.97
R9071:Sspo UTSW 6 48,433,982 (GRCm39) missense probably benign 0.00
R9133:Sspo UTSW 6 48,434,747 (GRCm39) missense possibly damaging 0.81
R9187:Sspo UTSW 6 48,472,223 (GRCm39) missense probably damaging 1.00
R9205:Sspo UTSW 6 48,432,806 (GRCm39) missense probably benign 0.03
R9213:Sspo UTSW 6 48,440,869 (GRCm39) missense possibly damaging 0.91
R9214:Sspo UTSW 6 48,440,869 (GRCm39) missense possibly damaging 0.91
R9215:Sspo UTSW 6 48,440,869 (GRCm39) missense possibly damaging 0.91
R9235:Sspo UTSW 6 48,466,718 (GRCm39) missense probably damaging 1.00
R9254:Sspo UTSW 6 48,464,928 (GRCm39) missense probably damaging 1.00
R9291:Sspo UTSW 6 48,473,330 (GRCm39) missense probably damaging 1.00
R9312:Sspo UTSW 6 48,445,396 (GRCm39) missense probably benign 0.00
R9357:Sspo UTSW 6 48,443,989 (GRCm39) missense possibly damaging 0.77
R9480:Sspo UTSW 6 48,470,820 (GRCm39) missense probably damaging 1.00
R9586:Sspo UTSW 6 48,458,039 (GRCm39) missense probably benign 0.03
R9660:Sspo UTSW 6 48,432,707 (GRCm39) missense probably damaging 1.00
R9661:Sspo UTSW 6 48,455,272 (GRCm39) nonsense probably null
R9728:Sspo UTSW 6 48,432,707 (GRCm39) missense probably damaging 1.00
R9776:Sspo UTSW 6 48,439,269 (GRCm39) missense probably benign 0.00
RF009:Sspo UTSW 6 48,436,919 (GRCm39) nonsense probably null
X0060:Sspo UTSW 6 48,457,728 (GRCm39) missense probably damaging 1.00
X0060:Sspo UTSW 6 48,443,228 (GRCm39) missense probably damaging 1.00
X0063:Sspo UTSW 6 48,474,356 (GRCm39) missense probably damaging 0.96
X0065:Sspo UTSW 6 48,438,618 (GRCm39) missense probably benign 0.00
Z1176:Sspo UTSW 6 48,458,227 (GRCm39) missense probably damaging 1.00
Z1177:Sspo UTSW 6 48,467,824 (GRCm39) missense probably damaging 1.00
Z1177:Sspo UTSW 6 48,467,482 (GRCm39) nonsense probably null
Z1177:Sspo UTSW 6 48,450,369 (GRCm39) missense probably damaging 0.99
Z1177:Sspo UTSW 6 48,447,918 (GRCm39) missense probably benign 0.16
Z1177:Sspo UTSW 6 48,441,750 (GRCm39) missense possibly damaging 0.72
Z1177:Sspo UTSW 6 48,433,960 (GRCm39) missense probably benign 0.31
Z1186:Sspo UTSW 6 48,445,441 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- GCCTTGCTTTGCATAACGC -3'
(R):5'- GAAAAGACCACTGGAGGCTCTG -3'

Sequencing Primer
(F):5'- TGCATAACGCCTAATGCTGG -3'
(R):5'- TCCACTGAGAGACAACAGAAGTCAG -3'
Posted On 2016-04-27