Mutagenetix > Incidental Mutation

Incidental Mutation 'R4969:Col6a5'

384279

Institutional Source

Beutler Lab

Gene Symbol

Col6a5

Ensembl Gene

ENSMUSG00000091345

Gene Name

collagen, type VI, alpha 5

Synonyms

Gm7455, Col6a5

Accession Numbers

Essential gene?

Probably essential (E-score: 0.839) question?

Stock #

R4969 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

105733277-105837842 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 105741806 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 2371 (T2371I)

Ref Sequence

ENSEMBL: ENSMUSP00000139398 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165165] [ENSMUST00000190193]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000165165
AA Change: T2371I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000131146
Gene: ENSMUSG00000091345
AA Change: T2371I

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
VWA	28	200	1.8e-24	SMART
low complexity region	222	248	N/A	INTRINSIC
VWA	266	439	2.23e-20	SMART
VWA	472	649	6.84e-39	SMART
VWA	658	834	1.52e-45	SMART
VWA	844	1024	2.44e-44	SMART
VWA	1035	1208	2.95e-20	SMART
Pfam:Collagen	1425	1478	3.3e-8	PFAM
low complexity region	1493	1508	N/A	INTRINSIC
low complexity region	1535	1552	N/A	INTRINSIC
Pfam:Collagen	1555	1616	9.6e-10	PFAM
low complexity region	1711	1730	N/A	INTRINSIC
low complexity region	1739	1757	N/A	INTRINSIC
VWA	1788	1964	1.99e-17	SMART
VWA	1994	2173	5.98e-21	SMART
VWA	2319	2513	4.4e-19	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000190193
AA Change: T2371I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000139398
Gene: ENSMUSG00000091345
AA Change: T2371I

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
VWA	28	200	1.1e-26	SMART
low complexity region	222	248	N/A	INTRINSIC
VWA	266	439	1.4e-22	SMART
VWA	472	649	4.4e-41	SMART
VWA	658	834	9.5e-48	SMART
VWA	844	1024	1.6e-46	SMART
VWA	1035	1208	1.9e-22	SMART
Pfam:Collagen	1425	1478	1.2e-6	PFAM
Pfam:Collagen	1457	1530	5.9e-6	PFAM
low complexity region	1535	1552	N/A	INTRINSIC
Pfam:Collagen	1555	1616	3.6e-8	PFAM
Pfam:Collagen	1631	1691	8.4e-6	PFAM
Pfam:Collagen	1706	1764	6.6e-6	PFAM
VWA	1788	1964	1.2e-19	SMART
VWA	1994	2173	3.7e-23	SMART
VWA	2319	2513	2.8e-21	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the collagen superfamily of proteins. The encoded protein contains multiple von Willebrand factor A-like domains and may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Polymorphisms in this gene may be linked to dermal phenotypes, such as eczema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc8	A	G	7: 45,754,943 (GRCm39)	I1525T	probably benign	Het
Agtpbp1	A	G	13: 59,648,392 (GRCm39)	V476A	probably benign	Het
Aipl1	A	G	11: 71,922,256 (GRCm39)	I151T	probably benign	Het
Apc	C	T	18: 34,445,971 (GRCm39)	R938*	probably null	Het
Atp2a2	A	G	5: 122,596,554 (GRCm39)	F855S	possibly damaging	Het
Axdnd1	T	C	1: 156,223,075 (GRCm39)	T261A	possibly damaging	Het
Cbfa2t2	A	G	2: 154,365,900 (GRCm39)	D370G	probably damaging	Het
Cep350	T	C	1: 155,736,025 (GRCm39)	I2964V	probably damaging	Het
Clec16a	T	C	16: 10,386,375 (GRCm39)	V158A	probably damaging	Het
Cpne8	T	C	15: 90,503,929 (GRCm39)	T79A	probably damaging	Het
Cyp2b13	A	G	7: 25,780,413 (GRCm39)	R145G	probably damaging	Het
Disc1	G	A	8: 125,851,289 (GRCm39)	W391*	probably null	Het
Dnah8	G	A	17: 30,941,988 (GRCm39)	V1745I	probably damaging	Het
Dsp	T	C	13: 38,376,886 (GRCm39)	V1557A	probably benign	Het
Egfem1	T	A	3: 29,637,145 (GRCm39)	Y194N	probably damaging	Het
Eif4a3	A	G	11: 119,179,705 (GRCm39)	Y361H	probably damaging	Het
Esd	T	A	14: 74,982,153 (GRCm39)	S189R	possibly damaging	Het
Fancf	A	T	7: 51,511,196 (GRCm39)	Y269*	probably null	Het
Fbln2	A	T	6: 91,248,569 (GRCm39)	H1078L	possibly damaging	Het
Fbxw13	A	G	9: 109,010,592 (GRCm39)		probably null	Het
Fcgr2b	A	T	1: 170,790,941 (GRCm39)	V284D	probably benign	Het
Fuz	G	A	7: 44,549,718 (GRCm39)	G363R	probably damaging	Het
Gas7	A	G	11: 67,574,234 (GRCm39)	E403G	probably damaging	Het
Gnl1	A	G	17: 36,291,581 (GRCm39)	D49G	possibly damaging	Het
Gucy2g	A	G	19: 55,214,445 (GRCm39)	V561A	probably benign	Het
H1f7	A	T	15: 98,154,216 (GRCm39)	V311E	unknown	Het
Hebp2	T	C	10: 18,420,122 (GRCm39)	T104A	probably benign	Het
Ighv1-19	T	A	12: 114,672,377 (GRCm39)	Q80L	probably benign	Het
Kctd19	T	A	8: 106,122,959 (GRCm39)		probably null	Het
Kdm3b	T	C	18: 34,955,428 (GRCm39)	L905P	probably damaging	Het
Klkb1	T	C	8: 45,735,814 (GRCm39)	D183G	probably benign	Het
Krt6b	T	C	15: 101,588,460 (GRCm39)	R67G	possibly damaging	Het
Krt75	T	C	15: 101,482,248 (GRCm39)	I7V	probably benign	Het
Lpo	A	T	11: 87,697,751 (GRCm39)	N685K	probably benign	Het
Mroh7	A	T	4: 106,538,070 (GRCm39)	I1202N	probably benign	Het
Muc4	T	A	16: 32,754,572 (GRCm38)	M1482K	probably benign	Het
Mylk	T	A	16: 34,791,810 (GRCm39)	V1494E	probably damaging	Het
Neurl4	A	G	11: 69,801,913 (GRCm39)	D17G	probably damaging	Het
Nkx6-3	A	G	8: 23,647,725 (GRCm39)	Y228C	probably damaging	Het
Nprl2	G	A	9: 107,420,273 (GRCm39)		probably null	Het
Nxf1	A	G	19: 8,739,669 (GRCm39)		probably null	Het
Or4a39	A	G	2: 89,236,770 (GRCm39)	F218L	probably benign	Het
Or52u1	A	T	7: 104,237,730 (GRCm39)	N240Y	probably damaging	Het
Pde3b	A	G	7: 114,118,847 (GRCm39)	E662G	possibly damaging	Het
Plekhm3	G	A	1: 64,977,078 (GRCm39)	R131C	probably damaging	Het
Plpp7	T	C	2: 31,985,950 (GRCm39)	S43P	probably benign	Het
Pramel5	A	G	4: 143,998,187 (GRCm39)	L352P	probably damaging	Het
Prepl	T	C	17: 85,395,902 (GRCm39)	S27G	probably benign	Het
Ptprd	A	G	4: 76,051,542 (GRCm39)	I227T	probably damaging	Het
Pttg1ip	C	T	10: 77,419,854 (GRCm39)	Q6*	probably null	Het
Rgl1	C	T	1: 152,424,813 (GRCm39)		probably null	Het
Riiad1	C	A	3: 94,380,173 (GRCm39)	G41*	probably null	Het
Rnf214	C	T	9: 45,807,486 (GRCm39)	R239H	probably damaging	Het
Rpap3	C	T	15: 97,584,407 (GRCm39)	V346I	probably benign	Het
Scaf4	G	A	16: 90,048,831 (GRCm39)	Q328*	probably null	Het
Sec23a	A	G	12: 59,051,274 (GRCm39)		probably null	Het
Slc5a8	T	A	10: 88,740,774 (GRCm39)		probably null	Het
Slc9a8	A	G	2: 167,288,449 (GRCm39)	T183A	probably benign	Het
Sod3	A	T	5: 52,525,736 (GRCm39)	H145L	probably damaging	Het
Sp4	A	G	12: 118,263,341 (GRCm39)	V235A	probably damaging	Het
Spp1	A	C	5: 104,588,153 (GRCm39)	E185A	possibly damaging	Het
Susd1	A	T	4: 59,351,679 (GRCm39)	W461R	probably benign	Het
Svil	A	C	18: 5,095,516 (GRCm39)	K1124Q	probably damaging	Het
Tdrd12	A	T	7: 35,186,720 (GRCm39)		probably null	Het
Terb1	A	T	8: 105,221,795 (GRCm39)	N168K	probably benign	Het
Tnnt1	A	G	7: 4,510,573 (GRCm39)	L216P	probably damaging	Het
Ttc28	A	G	5: 111,424,121 (GRCm39)	K1463E	probably damaging	Het
Twf2	T	G	9: 106,089,098 (GRCm39)		probably null	Het
Urb1	C	T	16: 90,602,299 (GRCm39)	R90Q	probably damaging	Het
Wdr45b	A	T	11: 121,219,650 (GRCm39)	C299*	probably null	Het
Wrap73	A	G	4: 154,237,138 (GRCm39)	S54G	probably damaging	Het
Zeb2	T	C	2: 44,888,931 (GRCm39)	K323R	probably damaging	Het
Zfp410	T	C	12: 84,378,582 (GRCm39)	I302T	possibly damaging	Het

Other mutations in Col6a5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00232:Col6a5	APN	9	105,759,882 (GRCm39)	missense	probably damaging	1.00
IGL01462:Col6a5	APN	9	105,823,274 (GRCm39)	missense	unknown
IGL01530:Col6a5	APN	9	105,792,385 (GRCm39)	splice site	probably benign
IGL01717:Col6a5	APN	9	105,817,472 (GRCm39)	missense	unknown
IGL01859:Col6a5	APN	9	105,808,160 (GRCm39)	nonsense	probably null
IGL01945:Col6a5	APN	9	105,805,489 (GRCm39)	missense	unknown
IGL01985:Col6a5	APN	9	105,814,482 (GRCm39)	missense	unknown
IGL02128:Col6a5	APN	9	105,817,093 (GRCm39)	missense	unknown
IGL02170:Col6a5	APN	9	105,805,621 (GRCm39)	missense	unknown
IGL02224:Col6a5	APN	9	105,741,534 (GRCm39)	missense	probably damaging	1.00
IGL02246:Col6a5	APN	9	105,788,306 (GRCm39)	nonsense	probably null
IGL02304:Col6a5	APN	9	105,805,613 (GRCm39)	missense	unknown
IGL02338:Col6a5	APN	9	105,755,829 (GRCm39)	missense	probably damaging	1.00
IGL02375:Col6a5	APN	9	105,783,312 (GRCm39)	missense	unknown
IGL02660:Col6a5	APN	9	105,814,085 (GRCm39)	missense	unknown
IGL02829:Col6a5	APN	9	105,811,506 (GRCm39)	missense	unknown
IGL02882:Col6a5	APN	9	105,811,520 (GRCm39)	missense	unknown
IGL02973:Col6a5	APN	9	105,803,020 (GRCm39)	missense	unknown
IGL03089:Col6a5	APN	9	105,811,038 (GRCm39)	missense	unknown
IGL03100:Col6a5	APN	9	105,814,512 (GRCm39)	missense	unknown
IGL03257:Col6a5	APN	9	105,759,072 (GRCm39)	missense	possibly damaging	0.95
FR4340:Col6a5	UTSW	9	105,811,373 (GRCm39)	missense	unknown
FR4342:Col6a5	UTSW	9	105,811,373 (GRCm39)	missense	unknown
FR4589:Col6a5	UTSW	9	105,811,373 (GRCm39)	missense	unknown
PIT4131001:Col6a5	UTSW	9	105,759,113 (GRCm39)	missense	probably damaging	0.98
R0147:Col6a5	UTSW	9	105,802,993 (GRCm39)	missense	unknown
R0549:Col6a5	UTSW	9	105,781,778 (GRCm39)	splice site	probably benign
R0622:Col6a5	UTSW	9	105,803,051 (GRCm39)	missense	unknown
R0628:Col6a5	UTSW	9	105,789,649 (GRCm39)	splice site	probably null
R0635:Col6a5	UTSW	9	105,805,805 (GRCm39)	missense	unknown
R0644:Col6a5	UTSW	9	105,825,523 (GRCm39)	critical splice donor site	probably null
R0828:Col6a5	UTSW	9	105,739,263 (GRCm39)	critical splice acceptor site	probably null
R0972:Col6a5	UTSW	9	105,817,484 (GRCm39)	missense	unknown
R1065:Col6a5	UTSW	9	105,758,982 (GRCm39)	missense	probably damaging	0.99
R1142:Col6a5	UTSW	9	105,811,516 (GRCm39)	missense	unknown
R1169:Col6a5	UTSW	9	105,774,173 (GRCm39)	splice site	probably null
R1522:Col6a5	UTSW	9	105,817,193 (GRCm39)	missense	unknown
R1646:Col6a5	UTSW	9	105,739,948 (GRCm39)	nonsense	probably null
R1719:Col6a5	UTSW	9	105,808,492 (GRCm39)	missense	unknown
R1759:Col6a5	UTSW	9	105,808,045 (GRCm39)	missense	unknown
R1780:Col6a5	UTSW	9	105,814,077 (GRCm39)	missense	unknown
R1812:Col6a5	UTSW	9	105,805,253 (GRCm39)	missense	unknown
R1838:Col6a5	UTSW	9	105,742,032 (GRCm39)	missense	probably benign	0.28
R1839:Col6a5	UTSW	9	105,742,032 (GRCm39)	missense	probably benign	0.28
R1863:Col6a5	UTSW	9	105,817,400 (GRCm39)	missense	unknown
R1900:Col6a5	UTSW	9	105,808,412 (GRCm39)	missense	unknown
R1951:Col6a5	UTSW	9	105,814,156 (GRCm39)	missense	unknown
R2024:Col6a5	UTSW	9	105,814,193 (GRCm39)	missense	unknown
R2126:Col6a5	UTSW	9	105,822,799 (GRCm39)	missense	unknown
R2319:Col6a5	UTSW	9	105,814,417 (GRCm39)	missense	unknown
R2344:Col6a5	UTSW	9	105,805,736 (GRCm39)	missense	unknown
R2483:Col6a5	UTSW	9	105,741,347 (GRCm39)	missense	probably damaging	1.00
R3176:Col6a5	UTSW	9	105,788,306 (GRCm39)	nonsense	probably null
R3276:Col6a5	UTSW	9	105,788,306 (GRCm39)	nonsense	probably null
R3438:Col6a5	UTSW	9	105,752,991 (GRCm39)	missense	possibly damaging	0.88
R3791:Col6a5	UTSW	9	105,741,868 (GRCm39)	missense	probably damaging	0.99
R3840:Col6a5	UTSW	9	105,805,810 (GRCm39)	missense	unknown
R3886:Col6a5	UTSW	9	105,808,129 (GRCm39)	missense	unknown
R3941:Col6a5	UTSW	9	105,817,033 (GRCm39)	missense	unknown
R4194:Col6a5	UTSW	9	105,823,113 (GRCm39)	missense	unknown
R4399:Col6a5	UTSW	9	105,766,164 (GRCm39)	missense	possibly damaging	0.75
R4421:Col6a5	UTSW	9	105,805,672 (GRCm39)	missense	unknown
R4450:Col6a5	UTSW	9	105,781,720 (GRCm39)	missense	unknown
R4491:Col6a5	UTSW	9	105,817,211 (GRCm39)	missense	unknown
R4582:Col6a5	UTSW	9	105,739,963 (GRCm39)	missense	probably benign	0.17
R4693:Col6a5	UTSW	9	105,814,371 (GRCm39)	missense	unknown
R4787:Col6a5	UTSW	9	105,808,280 (GRCm39)	missense	unknown
R4789:Col6a5	UTSW	9	105,814,534 (GRCm39)	missense	unknown
R4791:Col6a5	UTSW	9	105,807,983 (GRCm39)	missense	unknown
R4792:Col6a5	UTSW	9	105,807,983 (GRCm39)	missense	unknown
R4817:Col6a5	UTSW	9	105,811,497 (GRCm39)	missense	unknown
R4854:Col6a5	UTSW	9	105,775,950 (GRCm39)	missense	probably benign	0.18
R4927:Col6a5	UTSW	9	105,811,163 (GRCm39)	missense	unknown
R5037:Col6a5	UTSW	9	105,805,337 (GRCm39)	missense	unknown
R5118:Col6a5	UTSW	9	105,814,204 (GRCm39)	missense	unknown
R5144:Col6a5	UTSW	9	105,766,482 (GRCm39)	missense	probably damaging	1.00
R5145:Col6a5	UTSW	9	105,811,444 (GRCm39)	missense	unknown
R5160:Col6a5	UTSW	9	105,808,208 (GRCm39)	missense	unknown
R5182:Col6a5	UTSW	9	105,734,531 (GRCm39)	nonsense	probably null
R5234:Col6a5	UTSW	9	105,741,404 (GRCm39)	missense	probably damaging	1.00
R5252:Col6a5	UTSW	9	105,817,489 (GRCm39)	missense	unknown
R5290:Col6a5	UTSW	9	105,823,282 (GRCm39)	missense	unknown
R5313:Col6a5	UTSW	9	105,822,743 (GRCm39)	missense	unknown
R5321:Col6a5	UTSW	9	105,805,664 (GRCm39)	missense	unknown
R5466:Col6a5	UTSW	9	105,808,282 (GRCm39)	missense	unknown
R5540:Col6a5	UTSW	9	105,739,975 (GRCm39)	missense	probably benign	0.44
R5669:Col6a5	UTSW	9	105,803,197 (GRCm39)	missense	unknown
R5789:Col6a5	UTSW	9	105,741,807 (GRCm39)	missense	possibly damaging	0.91
R5801:Col6a5	UTSW	9	105,825,566 (GRCm39)	missense	unknown
R5827:Col6a5	UTSW	9	105,805,319 (GRCm39)	nonsense	probably null
R5839:Col6a5	UTSW	9	105,822,592 (GRCm39)	critical splice donor site	probably null
R5908:Col6a5	UTSW	9	105,740,000 (GRCm39)	missense	possibly damaging	0.88
R5970:Col6a5	UTSW	9	105,823,046 (GRCm39)	missense	unknown
R6045:Col6a5	UTSW	9	105,803,117 (GRCm39)	missense	unknown
R6107:Col6a5	UTSW	9	105,769,471 (GRCm39)	nonsense	probably null
R6168:Col6a5	UTSW	9	105,752,986 (GRCm39)	critical splice donor site	probably null
R6315:Col6a5	UTSW	9	105,759,169 (GRCm39)	missense	probably damaging	1.00
R6317:Col6a5	UTSW	9	105,766,266 (GRCm39)	missense	probably damaging	1.00
R6414:Col6a5	UTSW	9	105,769,465 (GRCm39)	splice site	probably null
R6434:Col6a5	UTSW	9	105,814,544 (GRCm39)	missense	unknown
R6456:Col6a5	UTSW	9	105,822,676 (GRCm39)	missense	unknown
R6698:Col6a5	UTSW	9	105,811,374 (GRCm39)	missense	unknown
R6876:Col6a5	UTSW	9	105,814,506 (GRCm39)	missense	unknown
R6882:Col6a5	UTSW	9	105,817,469 (GRCm39)	nonsense	probably null
R6928:Col6a5	UTSW	9	105,817,118 (GRCm39)	missense	unknown
R7024:Col6a5	UTSW	9	105,789,674 (GRCm39)	nonsense	probably null
R7038:Col6a5	UTSW	9	105,822,937 (GRCm39)	missense	unknown
R7082:Col6a5	UTSW	9	105,808,438 (GRCm39)	missense	unknown
R7158:Col6a5	UTSW	9	105,741,407 (GRCm39)	missense	possibly damaging	0.90
R7211:Col6a5	UTSW	9	105,805,363 (GRCm39)	missense	unknown
R7431:Col6a5	UTSW	9	105,805,468 (GRCm39)	missense	unknown
R7440:Col6a5	UTSW	9	105,758,630 (GRCm39)	nonsense	probably null
R7502:Col6a5	UTSW	9	105,753,075 (GRCm39)	missense	probably benign	0.05
R7577:Col6a5	UTSW	9	105,741,887 (GRCm39)	nonsense	probably null
R7582:Col6a5	UTSW	9	105,822,625 (GRCm39)	missense	unknown
R7641:Col6a5	UTSW	9	105,758,625 (GRCm39)	nonsense	probably null
R7762:Col6a5	UTSW	9	105,808,523 (GRCm39)	missense	unknown
R7793:Col6a5	UTSW	9	105,775,934 (GRCm39)	missense	probably damaging	1.00
R7821:Col6a5	UTSW	9	105,741,458 (GRCm39)	missense	probably damaging	1.00
R7848:Col6a5	UTSW	9	105,805,385 (GRCm39)	missense	unknown
R7897:Col6a5	UTSW	9	105,766,382 (GRCm39)	missense	possibly damaging	0.96
R7904:Col6a5	UTSW	9	105,805,720 (GRCm39)	missense	unknown
R7960:Col6a5	UTSW	9	105,823,049 (GRCm39)	missense	unknown
R8015:Col6a5	UTSW	9	105,758,940 (GRCm39)	missense	possibly damaging	0.65
R8100:Col6a5	UTSW	9	105,755,839 (GRCm39)	missense	probably damaging	1.00
R8131:Col6a5	UTSW	9	105,778,815 (GRCm39)	missense	unknown
R8418:Col6a5	UTSW	9	105,755,821 (GRCm39)	missense	probably damaging	1.00
R8425:Col6a5	UTSW	9	105,823,156 (GRCm39)	missense	unknown
R8678:Col6a5	UTSW	9	105,811,551 (GRCm39)	missense	unknown
R8690:Col6a5	UTSW	9	105,759,796 (GRCm39)	missense	probably damaging	0.97
R8847:Col6a5	UTSW	9	105,741,472 (GRCm39)	missense	possibly damaging	0.81
R8946:Col6a5	UTSW	9	105,822,833 (GRCm39)	missense	unknown
R8947:Col6a5	UTSW	9	105,822,833 (GRCm39)	missense	unknown
R8949:Col6a5	UTSW	9	105,822,833 (GRCm39)	missense	unknown
R8950:Col6a5	UTSW	9	105,822,833 (GRCm39)	missense	unknown
R9089:Col6a5	UTSW	9	105,766,142 (GRCm39)	missense	probably damaging	1.00
R9118:Col6a5	UTSW	9	105,755,853 (GRCm39)	splice site	probably benign
R9169:Col6a5	UTSW	9	105,822,596 (GRCm39)	missense	unknown
R9177:Col6a5	UTSW	9	105,808,152 (GRCm39)	missense	unknown
R9180:Col6a5	UTSW	9	105,739,178 (GRCm39)	missense	probably damaging	0.99
R9205:Col6a5	UTSW	9	105,755,837 (GRCm39)	missense	probably damaging	1.00
R9214:Col6a5	UTSW	9	105,758,940 (GRCm39)	missense	possibly damaging	0.65
R9224:Col6a5	UTSW	9	105,814,594 (GRCm39)	missense	unknown
R9279:Col6a5	UTSW	9	105,758,976 (GRCm39)	missense	probably damaging	1.00
R9383:Col6a5	UTSW	9	105,803,110 (GRCm39)	missense	unknown
R9427:Col6a5	UTSW	9	105,816,992 (GRCm39)	missense	unknown
R9488:Col6a5	UTSW	9	105,741,788 (GRCm39)	missense	probably damaging	1.00
R9494:Col6a5	UTSW	9	105,822,732 (GRCm39)	missense	unknown
R9659:Col6a5	UTSW	9	105,811,034 (GRCm39)	missense	unknown
R9749:Col6a5	UTSW	9	105,739,190 (GRCm39)	missense	probably damaging	0.98
RF013:Col6a5	UTSW	9	105,755,796 (GRCm39)	frame shift	probably null
X0054:Col6a5	UTSW	9	105,792,357 (GRCm39)	missense	unknown
X0058:Col6a5	UTSW	9	105,758,977 (GRCm39)	nonsense	probably null
Z1088:Col6a5	UTSW	9	105,803,266 (GRCm39)	missense	unknown
Z1177:Col6a5	UTSW	9	105,807,984 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGTCAACTGTCCACTGAAGGG -3'
(R):5'- CTGTTGCAGAACATGAAAGTCATG -3'

Sequencing Primer
(F):5'- ACTGTCCACTGAAGGGCATGC -3'
(R):5'- AGAAGAACTGGGTCTTGCTC -3'

Posted On

2016-04-27