|Institutional Source||Beutler Lab|
|Gene Name||H1 histone family, member N, testis-specific|
|Is this an essential gene?||Possibly non essential (E-score: 0.390)|
|Stock #||R4969 (G1)|
|Chromosomal Location||98255986-98257293 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 98256335 bp|
|Amino Acid Change||Valine to Glutamic Acid at position 311 (V311E)|
|Ref Sequence||ENSEMBL: ENSMUSP00000127616 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000060855]|
AA Change: V311E
AA Change: V311E
|Coding Region Coverage||
FUNCTION: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. This gene encodes a testis specific protein that is required for spermatogenesis and male fertility. [provided by RefSeq, Oct 2015]
PHENOTYPE: Homozygous null male mice display reduced fertility with asthenozoospermia, oligozoospermia, and teratozoospermia. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in H1fnt||
(F):5'- AAGACTTTGTGCATGCTTTTCC -3'
(R):5'- ACCTAGTGCGTTCCAAAGCC -3'
(F):5'- GAGCGTTGTCCAGAGCTG -3'
(R):5'- TTCCAAAGCCCGGGAACAGG -3'