Mutagenetix > Incidental Mutation

Incidental Mutation 'R4980:Cdc14a'

384732

Institutional Source

Beutler Lab

Gene Symbol

Cdc14a

Ensembl Gene

ENSMUSG00000033502

Gene Name

CDC14 cell division cycle 14A

Synonyms

A830059A17Rik, CDC14a1, CDC14A2, Cdc14

MMRRC Submission

042575-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.229) question?

Stock #

R4980 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

116066202-116222390 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 116186506 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 92 (R92*)

Ref Sequence

ENSEMBL: ENSMUSP00000102100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090464] [ENSMUST00000106491] [ENSMUST00000197830]

AlphaFold

Q6GQT0

Predicted Effect

probably null
Transcript: ENSMUST00000090464
AA Change: R92*

SMART Domains

Protein: ENSMUSP00000087950
Gene: ENSMUSG00000033502
AA Change: R92*

Domain	Start	End	E-Value	Type
Pfam:DSPn	13	153	1.6e-66	PFAM
Pfam:Y_phosphatase	210	324	1.1e-7	PFAM
Pfam:DSPc	214	328	1.8e-14	PFAM
low complexity region	539	558	N/A	INTRINSIC
low complexity region	573	595	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106491
AA Change: R92*

SMART Domains

Protein: ENSMUSP00000102100
Gene: ENSMUSG00000033502
AA Change: R92*

Domain	Start	End	E-Value	Type
Pfam:DSPn	12	121	9.5e-44	PFAM
Pfam:Y_phosphatase	160	274	6.4e-8	PFAM
Pfam:DSPc	160	280	2.8e-14	PFAM
low complexity region	490	509	N/A	INTRINSIC
low complexity region	524	546	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127704

Predicted Effect

probably benign
Transcript: ENSMUST00000197830

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,885,292 (GRCm39)	C189S	probably damaging	Het
Abcd3	A	T	3: 121,562,917 (GRCm39)		probably null	Het
Adar	T	C	3: 89,638,121 (GRCm39)	S2P	probably benign	Het
Amhr2	T	A	15: 102,362,765 (GRCm39)	S511T	probably benign	Het
Atg16l1	T	C	1: 87,694,553 (GRCm39)	S77P	possibly damaging	Het
Bltp1	T	C	3: 36,997,461 (GRCm39)	S1173P	probably damaging	Het
Cacna1i	T	C	15: 80,232,650 (GRCm39)	F218L	probably damaging	Het
Cd5l	A	T	3: 87,274,908 (GRCm39)	H149L	probably benign	Het
Col19a1	T	C	1: 24,565,564 (GRCm39)	T256A	unknown	Het
Col22a1	C	T	15: 71,673,792 (GRCm39)	A975T	unknown	Het
Cracd	A	T	5: 77,005,421 (GRCm39)	D594V	unknown	Het
Cyp20a1	T	C	1: 60,402,373 (GRCm39)	W153R	probably damaging	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Homo
Dpyd	T	C	3: 118,710,767 (GRCm39)	S392P	probably damaging	Het
Dst	A	T	1: 34,295,369 (GRCm39)	L957F	probably damaging	Het
Emilin2	G	T	17: 71,560,066 (GRCm39)	D970E	possibly damaging	Het
Fbn2	T	C	18: 58,143,703 (GRCm39)	E2784G	probably benign	Het
Fbxw10	T	C	11: 62,738,583 (GRCm39)	I159T	possibly damaging	Het
Fgfr2	T	C	7: 129,802,810 (GRCm39)	Y192C	probably damaging	Het
Gm7298	A	G	6: 121,736,198 (GRCm39)		probably null	Het
Igf2r	C	T	17: 12,922,247 (GRCm39)		probably null	Het
Ints4	T	C	7: 97,151,057 (GRCm39)		probably null	Het
Kcnf1	A	G	12: 17,225,012 (GRCm39)	F403S	possibly damaging	Het
Kif11	C	T	19: 37,375,819 (GRCm39)	Q211*	probably null	Het
Kif13a	A	G	13: 46,906,222 (GRCm39)	S574P	possibly damaging	Het
Kifc3	T	A	8: 95,853,177 (GRCm39)	T60S	probably benign	Het
Krt82	T	C	15: 101,453,534 (GRCm39)	D284G	possibly damaging	Het
Lancl1	T	C	1: 67,043,968 (GRCm39)	Y344C	probably benign	Het
Ltbp3	A	T	19: 5,803,955 (GRCm39)		probably null	Het
Map3k4	A	G	17: 12,490,958 (GRCm39)	F158L	probably damaging	Het
Mast2	A	G	4: 116,174,948 (GRCm39)	Y469H	probably damaging	Het
Mcf2l	T	G	8: 13,034,883 (GRCm39)	F97C	probably damaging	Het
Mpeg1	T	C	19: 12,438,904 (GRCm39)	S121P	probably damaging	Het
Mycbp2	A	T	14: 103,497,821 (GRCm39)		probably null	Het
Myt1l	A	T	12: 29,877,038 (GRCm39)	T230S	unknown	Het
Naa15	T	C	3: 51,366,173 (GRCm39)		probably null	Het
Nans	G	A	4: 46,492,764 (GRCm39)	R64Q	probably benign	Het
Nbea	C	T	3: 55,554,772 (GRCm39)		probably null	Het
Nbea	T	C	3: 55,860,466 (GRCm39)	E1870G	probably benign	Het
Ncapd3	T	C	9: 26,974,591 (GRCm39)	S688P	probably damaging	Het
Nedd4l	G	T	18: 65,213,131 (GRCm39)	E96*	probably null	Het
Or56a5	T	A	7: 104,793,431 (GRCm39)	Y23F	probably benign	Het
Or5b21	A	T	19: 12,839,384 (GRCm39)	M82L	probably benign	Het
Pdxk	A	G	10: 78,287,318 (GRCm39)	L81P	probably damaging	Het
Plec	T	A	15: 76,077,495 (GRCm39)	R269*	probably null	Het
Plec	T	C	15: 76,090,065 (GRCm39)	I92V	probably damaging	Het
Ppara	T	C	15: 85,671,434 (GRCm39)	Y112H	probably damaging	Het
Ppp2r2c	A	T	5: 37,109,732 (GRCm39)	M364L	probably benign	Het
Prag1	A	C	8: 36,606,740 (GRCm39)	H827P	probably damaging	Het
Ptprg	A	G	14: 12,154,421 (GRCm38)	N714S	probably benign	Het
Rbp2	G	A	9: 98,380,894 (GRCm39)	V67I	probably benign	Het
Rictor	T	A	15: 6,811,141 (GRCm39)	Y835N	probably damaging	Het
Ripply2	A	G	9: 86,901,747 (GRCm39)	Q91R	probably damaging	Het
Sap130	C	A	18: 31,782,699 (GRCm39)	H203Q	possibly damaging	Het
Sema4d	T	C	13: 51,865,270 (GRCm39)	Y358C	probably damaging	Het
Serbp1	G	A	6: 67,254,872 (GRCm39)	D294N	probably benign	Het
Slc16a10	A	G	10: 39,956,801 (GRCm39)	W113R	probably damaging	Het
Slc6a6	A	G	6: 91,703,041 (GRCm39)	Y138C	probably damaging	Het
Spag11a	T	C	8: 19,207,962 (GRCm39)	M1T	probably null	Het
Spata20	T	C	11: 94,375,435 (GRCm39)	M120V	probably damaging	Het
Stk32a	A	C	18: 43,447,113 (GRCm39)	K357Q	probably benign	Het
Tasor	T	A	14: 27,183,382 (GRCm39)	L614I	probably benign	Het
Trmo	A	T	4: 46,389,364 (GRCm39)	C10*	probably null	Het
Tsc22d1	T	G	14: 76,655,696 (GRCm39)	V643G	probably benign	Het
Vangl2	A	T	1: 171,837,132 (GRCm39)	L226I	probably damaging	Het
Vps35l	T	A	7: 118,406,232 (GRCm39)	D605E	probably damaging	Het
Wnt3a	A	T	11: 59,140,626 (GRCm39)	C297S	probably damaging	Het
Wwc1	T	C	11: 35,778,930 (GRCm39)	K301E	possibly damaging	Het
Zfhx4	C	T	3: 5,464,039 (GRCm39)	T1399I	possibly damaging	Het
Zswim4	C	T	8: 84,953,296 (GRCm39)		probably null	Het

Other mutations in Cdc14a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00801:Cdc14a	APN	3	116,088,493 (GRCm39)	nonsense	probably null
IGL01062:Cdc14a	APN	3	116,068,361 (GRCm39)	splice site	probably benign
IGL01584:Cdc14a	APN	3	116,186,474 (GRCm39)	nonsense	probably null
IGL03084:Cdc14a	APN	3	116,142,101 (GRCm39)	critical splice donor site	probably null
IGL03237:Cdc14a	APN	3	116,198,275 (GRCm39)	intron	probably benign
IGL03296:Cdc14a	APN	3	116,090,807 (GRCm39)	missense	probably benign	0.02
PIT4131001:Cdc14a	UTSW	3	116,122,310 (GRCm39)	missense	possibly damaging	0.66
R0707:Cdc14a	UTSW	3	116,087,362 (GRCm39)	splice site	probably benign
R0782:Cdc14a	UTSW	3	116,115,785 (GRCm39)	missense	probably damaging	1.00
R0835:Cdc14a	UTSW	3	116,122,171 (GRCm39)	missense	probably benign	0.12
R1363:Cdc14a	UTSW	3	116,087,509 (GRCm39)	small deletion	probably benign
R1507:Cdc14a	UTSW	3	116,087,646 (GRCm39)	missense	possibly damaging	0.47
R1545:Cdc14a	UTSW	3	116,087,373 (GRCm39)	critical splice donor site	probably null
R1795:Cdc14a	UTSW	3	116,092,122 (GRCm39)	missense	possibly damaging	0.81
R1797:Cdc14a	UTSW	3	116,115,843 (GRCm39)	missense	probably damaging	1.00
R1830:Cdc14a	UTSW	3	116,216,296 (GRCm39)	nonsense	probably null
R4229:Cdc14a	UTSW	3	116,087,413 (GRCm39)	missense	probably damaging	0.99
R4655:Cdc14a	UTSW	3	116,122,136 (GRCm39)	missense	probably damaging	1.00
R4769:Cdc14a	UTSW	3	116,088,399 (GRCm39)	critical splice donor site	probably null
R4870:Cdc14a	UTSW	3	116,217,109 (GRCm39)	missense	probably benign	0.30
R6228:Cdc14a	UTSW	3	116,144,862 (GRCm39)	missense	probably damaging	1.00
R6248:Cdc14a	UTSW	3	116,101,843 (GRCm39)	missense	probably benign	0.01
R6402:Cdc14a	UTSW	3	116,142,108 (GRCm39)	missense	probably damaging	1.00
R6749:Cdc14a	UTSW	3	116,090,807 (GRCm39)	missense	possibly damaging	0.68
R6852:Cdc14a	UTSW	3	116,122,325 (GRCm39)	missense	possibly damaging	0.94
R6996:Cdc14a	UTSW	3	116,122,355 (GRCm39)	missense	probably damaging	1.00
R7185:Cdc14a	UTSW	3	116,087,676 (GRCm39)	missense	probably benign
R7783:Cdc14a	UTSW	3	116,198,236 (GRCm39)	missense	probably damaging	1.00
R7896:Cdc14a	UTSW	3	116,088,482 (GRCm39)	missense	probably benign	0.00
R7991:Cdc14a	UTSW	3	116,101,887 (GRCm39)	missense	probably benign	0.01
R8049:Cdc14a	UTSW	3	116,087,577 (GRCm39)	missense	probably benign	0.33
R9163:Cdc14a	UTSW	3	116,122,213 (GRCm39)	missense	possibly damaging	0.95
R9434:Cdc14a	UTSW	3	116,217,092 (GRCm39)	missense	probably benign
R9526:Cdc14a	UTSW	3	116,087,509 (GRCm39)	small deletion	probably benign
R9662:Cdc14a	UTSW	3	116,088,484 (GRCm39)	missense	probably damaging	0.99
R9781:Cdc14a	UTSW	3	116,122,274 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACTCCGATGATGTTAGTCCAC -3'
(R):5'- AAAGTGAGCAGTAGAACCCC -3'

Sequencing Primer
(F):5'- CGATGATGTTAGTCCACTTTACG -3'
(R):5'- GGAATGTCCCAAATACCAGAAGCTTG -3'

Posted On

2016-05-10