Mutagenetix > Incidental Mutation

Incidental Mutation 'R4994:Kif14'

385066

Institutional Source

Beutler Lab

Gene Symbol

Kif14

Ensembl Gene

ENSMUSG00000041498

Gene Name

kinesin family member 14

Synonyms

N-3 kinesin, D1Ertd367e

MMRRC Submission

042588-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.947) question?

Stock #

R4994 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

136394081-136459249 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 136410697 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 668 (L668F)

Ref Sequence

ENSEMBL: ENSMUSP00000139698 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047817] [ENSMUST00000189413] [ENSMUST00000195274] [ENSMUST00000201676]

AlphaFold

L0N7N1

PDB Structure

Crystal structure of the mouse Kif14 motor domain [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000047817
AA Change: L618F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000044257
Gene: ENSMUSG00000041498
AA Change: L618F

Domain	Start	End	E-Value	Type
KISc	341	694	1.45e-180	SMART
FHA	809	861	1.46e-7	SMART
coiled coil region	911	1060	N/A	INTRINSIC
low complexity region	1169	1179	N/A	INTRINSIC
low complexity region	1548	1559	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000187963

Predicted Effect

probably damaging
Transcript: ENSMUST00000189413
AA Change: L668F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139698
Gene: ENSMUSG00000041498
AA Change: L668F

Domain	Start	End	E-Value	Type
low complexity region	278	290	N/A	INTRINSIC
KISc	391	744	1.45e-180	SMART
FHA	859	911	1.46e-7	SMART
coiled coil region	961	1110	N/A	INTRINSIC
low complexity region	1219	1229	N/A	INTRINSIC
low complexity region	1598	1609	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000193587

Predicted Effect

probably benign
Transcript: ENSMUST00000195274

SMART Domains

Protein: ENSMUSP00000142040
Gene: ENSMUSG00000041498

Domain	Start	End	E-Value	Type
Pfam:Kinesin	29	69	3.7e-12	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195390

Predicted Effect

probably benign
Transcript: ENSMUST00000201676

SMART Domains

Protein: ENSMUSP00000144265
Gene: ENSMUSG00000041498

Domain	Start	End	E-Value	Type
low complexity region	278	290	N/A	INTRINSIC
KISc	391	497	3.7e-6	SMART

Meta Mutation Damage Score

0.9144

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

100% (79/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
PHENOTYPE: Mice homozygous for a spontaneous mutation or targeted allele exhibit severe brain malformations, neurological defects and hypomyelination. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030K09Rik	A	G	8: 73,208,962 (GRCm39)	E364G	probably benign	Het
4930505A04Rik	C	T	11: 30,376,349 (GRCm39)	V173M	probably damaging	Het
Abcb4	A	G	5: 8,978,524 (GRCm39)	T557A	probably damaging	Het
Acadm	C	T	3: 153,635,221 (GRCm39)	E298K	probably damaging	Het
Adrb3	T	C	8: 27,717,855 (GRCm39)		probably null	Het
Aldh1b1	A	G	4: 45,803,128 (GRCm39)	Y222C	possibly damaging	Het
Ankrd6	A	G	4: 32,860,387 (GRCm39)	Y19H	probably damaging	Het
Arhgap21	T	A	2: 20,854,701 (GRCm39)	T1554S	probably benign	Het
BC043934	T	C	9: 96,319,173 (GRCm39)		noncoding transcript	Het
Birc6	A	G	17: 74,901,319 (GRCm39)		probably benign	Het
Blm	A	C	7: 80,108,573 (GRCm39)	F1357C	probably benign	Het
Cd209a	T	C	8: 3,797,713 (GRCm39)		probably null	Het
Cdk7	G	A	13: 100,854,103 (GRCm39)	H129Y	probably damaging	Het
Clec14a	G	T	12: 58,315,070 (GRCm39)	P184Q	probably damaging	Het
Cma1	T	A	14: 56,179,128 (GRCm39)	I243F	probably damaging	Het
Cntnap3	G	T	13: 64,909,798 (GRCm39)	T769K	possibly damaging	Het
Col5a1	A	C	2: 27,922,751 (GRCm39)	K273T	possibly damaging	Het
Csnk1e	A	G	15: 79,309,129 (GRCm39)	Y266H	probably damaging	Het
Cyb5d1	A	G	11: 69,284,597 (GRCm39)	L185S	probably damaging	Het
Dennd5b	T	C	6: 148,942,998 (GRCm39)		probably null	Het
Dipk2a	C	A	9: 94,419,486 (GRCm39)	R148L	probably benign	Het
Drp2	G	A	X: 133,342,065 (GRCm39)	R567H	probably damaging	Homo
Dzank1	T	G	2: 144,364,486 (GRCm39)	D37A	probably damaging	Het
Echdc2	A	G	4: 108,022,825 (GRCm39)	I34V	probably benign	Het
Esm1	A	T	13: 113,349,965 (GRCm39)	R128S	probably benign	Het
Fbh1	T	A	2: 11,769,041 (GRCm39)	I251F	probably damaging	Het
Fbrsl1	A	G	5: 110,595,817 (GRCm39)	S73P	probably damaging	Het
Fbxo6	A	T	4: 148,233,948 (GRCm39)	S49R	probably damaging	Het
Gm17093	A	T	14: 44,756,779 (GRCm39)	Q82L	probably damaging	Het
Hmcn2	T	C	2: 31,348,067 (GRCm39)		probably null	Het
Hspa4l	C	A	3: 40,700,081 (GRCm39)		probably benign	Het
Il3ra	G	A	14: 14,351,080 (GRCm38)	A201T	probably benign	Het
Irx5	T	A	8: 93,087,409 (GRCm39)	V447E	probably damaging	Het
Lag3	T	A	6: 124,881,416 (GRCm39)	R519W	unknown	Het
Lgr4	A	G	2: 109,842,283 (GRCm39)	N756S	probably damaging	Het
Lingo4	A	G	3: 94,309,848 (GRCm39)	H262R	probably benign	Het
Lingo4	T	A	3: 94,310,308 (GRCm39)	H415Q	probably benign	Het
Lkaaear1	C	A	2: 181,339,376 (GRCm39)	G25*	probably null	Het
Maco1	A	G	4: 134,555,610 (GRCm39)	Y288H	probably damaging	Het
Marf1	T	C	16: 13,932,095 (GRCm39)	K1641E	probably benign	Het
Mtfmt	T	C	9: 65,351,133 (GRCm39)		probably benign	Het
Mtif3	G	A	5: 146,893,598 (GRCm39)	T203M	probably benign	Het
Mycbp2	A	G	14: 103,407,430 (GRCm39)	I2740T	probably benign	Het
Nrdc	A	G	4: 108,903,809 (GRCm39)	T720A	probably benign	Het
Or2d2	T	C	7: 106,728,271 (GRCm39)	T110A	probably benign	Het
Peak1	T	A	9: 56,148,560 (GRCm39)	D32V	possibly damaging	Het
Pigr	G	A	1: 130,769,554 (GRCm39)	D122N	probably benign	Het
Plekhg3	A	G	12: 76,612,311 (GRCm39)	R391G	possibly damaging	Het
Ppfia3	T	C	7: 44,990,542 (GRCm39)	D919G	probably damaging	Het
Pramel23	T	A	4: 143,424,939 (GRCm39)	Q168L	possibly damaging	Het
Rnase2b	T	A	14: 51,400,208 (GRCm39)	D96E	possibly damaging	Het
Rsf1	CGGCGGC	CGGCGGCCGCGGCGGC	7: 97,229,130 (GRCm39)		probably benign	Het
Rsf1	G	GACGGCGGCT	7: 97,229,116 (GRCm39)		probably benign	Het
Serpina3f	A	G	12: 104,186,615 (GRCm39)	T394A	probably benign	Het
Six3	A	G	17: 85,928,720 (GRCm39)	N18S	possibly damaging	Het
Slc12a5	T	A	2: 164,825,285 (GRCm39)		probably null	Het
Slc35f6	A	G	5: 30,805,427 (GRCm39)	N21S	probably damaging	Het
Slc39a6	A	T	18: 24,729,351 (GRCm39)	I454N	probably damaging	Het
Slc40a1	T	A	1: 45,948,824 (GRCm39)	E485D	probably damaging	Het
Sort1	G	A	3: 108,235,385 (GRCm39)	C255Y	probably damaging	Het
Stab2	T	A	10: 86,785,771 (GRCm39)	T624S	probably benign	Het
Stk33	T	A	7: 108,939,605 (GRCm39)	I99L	probably benign	Het
Taf4b	T	A	18: 15,031,100 (GRCm39)	I828N	probably damaging	Het
Terf2	T	C	8: 107,803,110 (GRCm39)		probably benign	Het
Timd4	C	T	11: 46,706,344 (GRCm39)	R49C	probably damaging	Het
Tpte	T	C	8: 22,808,362 (GRCm39)	S166P	probably benign	Het
Trabd2b	T	C	4: 114,264,052 (GRCm39)	L13P	probably benign	Het
Trappc11	A	T	8: 47,975,476 (GRCm39)	Y247*	probably null	Het
Trnt1	C	T	6: 106,755,853 (GRCm39)	Q303*	probably null	Het
Tspyl5	T	A	15: 33,687,201 (GRCm39)	Q248L	possibly damaging	Het
Ubxn7	A	T	16: 32,200,322 (GRCm39)	K337N	probably damaging	Het
Unc13a	T	A	8: 72,095,816 (GRCm39)	I1234F	probably benign	Het
Vmn1r127	A	T	7: 21,052,943 (GRCm39)	F282I	probably damaging	Het
Wdhd1	A	T	14: 47,506,111 (GRCm39)		probably null	Het
Zfp605	A	G	5: 110,275,352 (GRCm39)	K157E	probably damaging	Het
Zhx2	T	A	15: 57,684,755 (GRCm39)	D41E	probably benign	Het

Other mutations in Kif14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00159:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00160:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00164:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00310:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00330:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00335:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00434:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL00468:Kif14	APN	1	136,396,756 (GRCm39)	missense	probably benign	0.11
IGL01330:Kif14	APN	1	136,404,112 (GRCm39)	missense	probably damaging	0.99
IGL01530:Kif14	APN	1	136,406,157 (GRCm39)	splice site	probably benign
IGL01622:Kif14	APN	1	136,425,094 (GRCm39)	splice site	probably benign
IGL01689:Kif14	APN	1	136,447,380 (GRCm39)	missense	probably damaging	0.99
IGL02115:Kif14	APN	1	136,424,305 (GRCm39)	splice site	probably benign
IGL02252:Kif14	APN	1	136,406,130 (GRCm39)	missense	probably damaging	1.00
IGL02259:Kif14	APN	1	136,427,840 (GRCm39)	missense	probably benign
IGL02439:Kif14	APN	1	136,417,999 (GRCm39)	missense	probably damaging	1.00
IGL02590:Kif14	APN	1	136,423,742 (GRCm39)	missense	probably benign	0.00
IGL02606:Kif14	APN	1	136,424,331 (GRCm39)	missense	probably damaging	1.00
IGL03253:Kif14	APN	1	136,415,198 (GRCm39)	missense	probably damaging	0.97
R0106:Kif14	UTSW	1	136,407,662 (GRCm39)	splice site	probably benign
R0193:Kif14	UTSW	1	136,396,176 (GRCm39)	missense	probably benign	0.00
R0238:Kif14	UTSW	1	136,455,131 (GRCm39)	missense	probably damaging	0.99
R0238:Kif14	UTSW	1	136,455,131 (GRCm39)	missense	probably damaging	0.99
R0239:Kif14	UTSW	1	136,455,131 (GRCm39)	missense	probably damaging	0.99
R0239:Kif14	UTSW	1	136,455,131 (GRCm39)	missense	probably damaging	0.99
R0329:Kif14	UTSW	1	136,423,764 (GRCm39)	splice site	probably benign
R0346:Kif14	UTSW	1	136,395,898 (GRCm39)	missense	probably damaging	1.00
R0393:Kif14	UTSW	1	136,410,156 (GRCm39)	missense	probably damaging	1.00
R0519:Kif14	UTSW	1	136,396,885 (GRCm39)	missense	probably damaging	1.00
R0590:Kif14	UTSW	1	136,410,210 (GRCm39)	missense	probably damaging	0.97
R0633:Kif14	UTSW	1	136,455,043 (GRCm39)	missense	probably damaging	0.96
R0657:Kif14	UTSW	1	136,396,840 (GRCm39)	missense	probably benign	0.07
R0831:Kif14	UTSW	1	136,453,609 (GRCm39)	splice site	probably benign
R0971:Kif14	UTSW	1	136,447,392 (GRCm39)	missense	probably damaging	0.98
R1018:Kif14	UTSW	1	136,423,579 (GRCm39)	splice site	probably benign
R1520:Kif14	UTSW	1	136,431,062 (GRCm39)	missense	probably benign	0.00
R1713:Kif14	UTSW	1	136,455,202 (GRCm39)	missense	probably benign	0.00
R1728:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1728:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1728:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1728:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1728:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1728:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1728:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1729:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1729:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1729:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1729:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1729:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1729:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1729:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1730:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1730:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1730:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1730:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1730:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1730:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1730:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1739:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1739:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1739:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1739:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1739:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1739:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1739:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1762:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1762:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1762:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1762:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1762:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1762:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1762:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1783:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1783:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1783:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1783:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1783:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1783:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1783:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1784:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1784:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1784:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1784:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1784:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1784:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1784:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1785:Kif14	UTSW	1	136,396,713 (GRCm39)	missense	probably damaging	1.00
R1785:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign	0.00
R1785:Kif14	UTSW	1	136,418,070 (GRCm39)	missense	probably benign
R1785:Kif14	UTSW	1	136,431,169 (GRCm39)	missense	probably benign	0.10
R1785:Kif14	UTSW	1	136,443,699 (GRCm39)	missense	probably benign	0.04
R1785:Kif14	UTSW	1	136,453,521 (GRCm39)	missense	probably benign	0.03
R1785:Kif14	UTSW	1	136,396,017 (GRCm39)	missense	probably benign
R1872:Kif14	UTSW	1	136,414,096 (GRCm39)	missense	probably damaging	1.00
R2049:Kif14	UTSW	1	136,414,818 (GRCm39)	missense	probably benign
R2049:Kif14	UTSW	1	136,437,905 (GRCm39)	missense	possibly damaging	0.68
R2268:Kif14	UTSW	1	136,447,486 (GRCm39)	nonsense	probably null
R2373:Kif14	UTSW	1	136,407,583 (GRCm39)	missense	probably damaging	1.00
R3076:Kif14	UTSW	1	136,447,383 (GRCm39)	missense	possibly damaging	0.51
R3077:Kif14	UTSW	1	136,447,383 (GRCm39)	missense	possibly damaging	0.51
R3078:Kif14	UTSW	1	136,447,383 (GRCm39)	missense	possibly damaging	0.51
R4232:Kif14	UTSW	1	136,444,101 (GRCm39)	nonsense	probably null
R4246:Kif14	UTSW	1	136,401,126 (GRCm39)	missense	possibly damaging	0.80
R4247:Kif14	UTSW	1	136,401,126 (GRCm39)	missense	possibly damaging	0.80
R4250:Kif14	UTSW	1	136,401,126 (GRCm39)	missense	possibly damaging	0.80
R4672:Kif14	UTSW	1	136,449,016 (GRCm39)	missense	probably benign	0.00
R4672:Kif14	UTSW	1	136,449,017 (GRCm39)	missense	probably benign
R4890:Kif14	UTSW	1	136,414,868 (GRCm39)	missense	possibly damaging	0.91
R5102:Kif14	UTSW	1	136,444,141 (GRCm39)	missense	probably benign	0.00
R5185:Kif14	UTSW	1	136,455,207 (GRCm39)	nonsense	probably null
R5201:Kif14	UTSW	1	136,431,145 (GRCm39)	missense	probably benign	0.00
R5399:Kif14	UTSW	1	136,431,062 (GRCm39)	missense	probably benign	0.00
R5431:Kif14	UTSW	1	136,424,433 (GRCm39)	missense	possibly damaging	0.91
R5932:Kif14	UTSW	1	136,444,128 (GRCm39)	missense	probably benign	0.23
R6027:Kif14	UTSW	1	136,410,797 (GRCm39)	splice site	probably null
R6246:Kif14	UTSW	1	136,404,162 (GRCm39)	nonsense	probably null
R6331:Kif14	UTSW	1	136,443,724 (GRCm39)	missense	probably null	1.00
R6448:Kif14	UTSW	1	136,431,085 (GRCm39)	missense	probably damaging	0.99
R6453:Kif14	UTSW	1	136,410,042 (GRCm39)	splice site	probably null
R6475:Kif14	UTSW	1	136,455,149 (GRCm39)	missense	probably damaging	1.00
R6631:Kif14	UTSW	1	136,443,697 (GRCm39)	missense	probably benign	0.39
R6713:Kif14	UTSW	1	136,453,544 (GRCm39)	missense	probably benign
R7173:Kif14	UTSW	1	136,406,908 (GRCm39)	missense	probably damaging	0.98
R7174:Kif14	UTSW	1	136,448,995 (GRCm39)	missense	possibly damaging	0.67
R7241:Kif14	UTSW	1	136,396,491 (GRCm39)	missense	probably benign	0.41
R7674:Kif14	UTSW	1	136,396,558 (GRCm39)	missense	probably damaging	0.99
R7688:Kif14	UTSW	1	136,422,392 (GRCm39)	missense	probably damaging	1.00
R7711:Kif14	UTSW	1	136,399,191 (GRCm39)	missense	probably benign	0.10
R7722:Kif14	UTSW	1	136,396,033 (GRCm39)	missense	probably benign	0.00
R7763:Kif14	UTSW	1	136,444,121 (GRCm39)	missense	probably benign	0.00
R7882:Kif14	UTSW	1	136,443,763 (GRCm39)	missense	probably benign	0.43
R7882:Kif14	UTSW	1	136,399,314 (GRCm39)	critical splice donor site	probably null
R8077:Kif14	UTSW	1	136,399,186 (GRCm39)	missense	possibly damaging	0.87
R8101:Kif14	UTSW	1	136,404,090 (GRCm39)	missense	probably benign	0.14
R8308:Kif14	UTSW	1	136,443,651 (GRCm39)	missense	possibly damaging	0.90
R8338:Kif14	UTSW	1	136,422,416 (GRCm39)	missense	probably damaging	1.00
R8527:Kif14	UTSW	1	136,396,495 (GRCm39)	missense	possibly damaging	0.95
R8542:Kif14	UTSW	1	136,396,495 (GRCm39)	missense	possibly damaging	0.95
R8884:Kif14	UTSW	1	136,414,089 (GRCm39)	missense
R9435:Kif14	UTSW	1	136,401,174 (GRCm39)	missense	possibly damaging	0.92
R9499:Kif14	UTSW	1	136,455,219 (GRCm39)	missense	probably damaging	0.96
R9551:Kif14	UTSW	1	136,455,219 (GRCm39)	missense	probably damaging	0.96
R9577:Kif14	UTSW	1	136,399,138 (GRCm39)	missense	probably benign	0.00
X0021:Kif14	UTSW	1	136,418,014 (GRCm39)	missense	probably damaging	1.00
Z1176:Kif14	UTSW	1	136,427,754 (GRCm39)	critical splice acceptor site	probably null
Z1176:Kif14	UTSW	1	136,424,391 (GRCm39)	missense	probably damaging	0.97
Z1177:Kif14	UTSW	1	136,406,103 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGCGTCTTTATCTTTAGTACAGACTGC -3'
(R):5'- ACATATGTGCACAAACACGG -3'

Sequencing Primer
(F):5'- TCACACACTGCATTGGGGTTAAG -3'
(R):5'- TATGTGCACAAACACGGATACAC -3'

Posted On

2016-05-10