Mutagenetix > Incidental Mutation

Incidental Mutation 'R4989:Hydin'

386050

Institutional Source

Beutler Lab

Gene Symbol

Hydin

Ensembl Gene

ENSMUSG00000059854

Gene Name

HYDIN, axonemal central pair apparatus protein

Synonyms

1700034M11Rik, 4930545D19Rik, hy3, hyrh, hy-3

MMRRC Submission

042583-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.757) question?

Stock #

R4989 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

110993609-111336885 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 111290554 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 3338 (I3338F)

Ref Sequence

ENSEMBL: ENSMUSP00000046204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043141]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000043141
AA Change: I3338F

PolyPhen 2 Score 0.843 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000046204
Gene: ENSMUSG00000059854
AA Change: I3338F

Domain	Start	End	E-Value	Type
Pfam:Motile_Sperm	246	325	5.6e-8	PFAM
Pfam:ASH	559	659	9.4e-17	PFAM
low complexity region	788	798	N/A	INTRINSIC
Pfam:PapD-like	848	906	1.2e-6	PFAM
low complexity region	998	1024	N/A	INTRINSIC
low complexity region	1279	1292	N/A	INTRINSIC
internal_repeat_6	1317	1549	5.96e-5	PROSPERO
internal_repeat_5	1355	1502	3.23e-5	PROSPERO
low complexity region	1574	1590	N/A	INTRINSIC
internal_repeat_4	1712	1940	5.14e-6	PROSPERO
coiled coil region	1947	1977	N/A	INTRINSIC
low complexity region	2009	2020	N/A	INTRINSIC
low complexity region	2034	2049	N/A	INTRINSIC
SCOP:d1eq1a_	2305	2403	3e-4	SMART
low complexity region	2404	2419	N/A	INTRINSIC
coiled coil region	2543	2588	N/A	INTRINSIC
low complexity region	2636	2656	N/A	INTRINSIC
internal_repeat_7	2772	3008	8.1e-5	PROSPERO
low complexity region	3660	3670	N/A	INTRINSIC
low complexity region	3919	3934	N/A	INTRINSIC
internal_repeat_5	4046	4190	3.23e-5	PROSPERO
internal_repeat_2	4106	4251	6.03e-7	PROSPERO
internal_repeat_4	4317	4532	5.14e-6	PROSPERO
internal_repeat_3	4403	4689	2.05e-6	PROSPERO
internal_repeat_2	4549	4697	6.03e-7	PROSPERO
low complexity region	4951	4964	N/A	INTRINSIC

Meta Mutation Damage Score

0.2088

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

100% (115/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that may be involved in cilia motility. Mutations in this gene cause of autosomal recessive primary ciliary dyskinesia-5, a disorder characterized by the accumulation of cerebrospinal fluid within the ventricles of the brain. A duplicate copy of this gene has been found in humans on chromosome 1. [provided by RefSeq, Jan 2013]
PHENOTYPE: Mice homozygous for a mutation in this gene develop hydrocephaly after birth. Symptoms develop after 3-5 days. Affected animals usually die before 2 months of age. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted(1) Gene trapped(3) Transgenic(1) Spontaneous(2)

Other mutations in this stock

Total: 114 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
Acot6	A	G	12: 84,155,789 (GRCm39)	K246E	probably benign	Het
Actl11	T	A	9: 107,808,615 (GRCm39)	H979Q	probably damaging	Het
Adal	T	A	2: 120,986,030 (GRCm39)		probably benign	Het
Add2	G	A	6: 86,087,840 (GRCm39)	V596I	probably benign	Het
Adrb3	A	C	8: 27,717,798 (GRCm39)	M217R	probably damaging	Het
Ank1	T	C	8: 23,631,134 (GRCm39)		probably benign	Het
Ank2	A	T	3: 126,757,094 (GRCm39)	N1054K	possibly damaging	Het
Ankib1	T	C	5: 3,763,217 (GRCm39)	Y504C	probably damaging	Het
Ankrd29	T	C	18: 12,395,242 (GRCm39)	K217R	probably damaging	Het
Ankrd45	T	C	1: 160,982,876 (GRCm39)	V129A	probably damaging	Het
Aox4	A	T	1: 58,275,835 (GRCm39)	D389V	probably benign	Het
Aph1a	G	T	3: 95,802,843 (GRCm39)	G148W	probably damaging	Het
Arhgef26	G	A	3: 62,247,806 (GRCm39)	D297N	possibly damaging	Het
Atxn7l3b	C	A	10: 112,764,649 (GRCm39)		probably benign	Het
Auh	A	G	13: 52,995,065 (GRCm39)	S167P	probably damaging	Het
Bach1	A	T	16: 87,515,888 (GRCm39)	K143I	possibly damaging	Het
Bbx	T	A	16: 50,045,101 (GRCm39)	T487S	probably damaging	Het
Bche	T	G	3: 73,609,177 (GRCm39)	D83A	probably benign	Het
Bri3bp	G	T	5: 125,518,760 (GRCm39)		probably benign	Het
Cd3d	A	T	9: 44,896,296 (GRCm39)	E28D	probably damaging	Het
Cdc42bpa	A	G	1: 179,965,366 (GRCm39)	T1028A	probably damaging	Het
Ceacam23	A	G	7: 17,639,143 (GRCm39)		probably null	Het
Celsr2	T	C	3: 108,319,945 (GRCm39)	I956V	possibly damaging	Het
Cep95	A	T	11: 106,707,480 (GRCm39)		probably null	Het
Cic	G	T	7: 24,986,535 (GRCm39)	G1289C	probably damaging	Het
Cndp1	A	G	18: 84,650,025 (GRCm39)	Y223H	probably damaging	Het
Cops8	A	G	1: 90,538,724 (GRCm39)	D51G	probably damaging	Het
Csgalnact1	T	C	8: 68,913,623 (GRCm39)	E194G	probably benign	Het
Ctsm	A	G	13: 61,686,776 (GRCm39)	Y39H	probably damaging	Het
Dhrs2	T	C	14: 55,474,722 (GRCm39)	V119A	probably damaging	Het
Drosha	T	A	15: 12,935,093 (GRCm39)	M1336K	probably benign	Het
Dsp	G	A	13: 38,381,678 (GRCm39)	D2808N	possibly damaging	Het
Ehmt1	G	T	2: 24,767,509 (GRCm39)	P135T	probably damaging	Het
Epas1	A	G	17: 87,116,882 (GRCm39)	N184S	probably damaging	Het
Erich3	C	T	3: 154,454,025 (GRCm39)	T597I	possibly damaging	Het
F10	T	C	8: 13,105,698 (GRCm39)	V421A	probably damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fam227b	G	A	2: 125,958,043 (GRCm39)	P241S	probably damaging	Het
Fcgrt	C	T	7: 44,751,372 (GRCm39)	G192D	probably benign	Het
Fras1	A	G	5: 96,798,541 (GRCm39)	E1184G	possibly damaging	Het
Garre1	A	C	7: 33,945,225 (GRCm39)	Y552D	probably damaging	Het
Grn	C	A	11: 102,321,380 (GRCm39)		probably benign	Het
Gvin-ps5	A	T	7: 105,928,664 (GRCm39)		noncoding transcript	Het
Hadh	A	T	3: 131,029,197 (GRCm39)	L274*	probably null	Het
Hus1	T	C	11: 8,956,027 (GRCm39)	S169G	probably damaging	Het
Ighv11-1	T	G	12: 113,945,768 (GRCm39)	E28D	probably benign	Het
Kctd9	C	T	14: 67,966,805 (GRCm39)	T106I	probably damaging	Het
Krit1	A	T	5: 3,872,238 (GRCm39)	N421I	probably damaging	Het
Lamb1	A	G	12: 31,376,677 (GRCm39)	D1619G	probably damaging	Het
Lpar6	G	A	14: 73,476,147 (GRCm39)	C36Y	probably damaging	Het
Lrrc37a	G	C	11: 103,347,565 (GRCm39)	F3043L	unknown	Het
Lrrc46	A	T	11: 96,931,765 (GRCm39)	V19D	probably damaging	Het
Mapkap1	G	T	2: 34,471,303 (GRCm39)	S197I	probably damaging	Het
Mrps35	A	C	6: 146,961,645 (GRCm39)	K173N	possibly damaging	Het
Mtf2	A	G	5: 108,220,894 (GRCm39)		probably benign	Het
Ncf1	A	C	5: 134,252,267 (GRCm39)	D261E	probably damaging	Het
Notch1	C	T	2: 26,371,193 (GRCm39)	E298K	probably damaging	Het
Nrxn1	A	T	17: 90,928,274 (GRCm39)		probably benign	Het
Or13a18	C	A	7: 140,190,304 (GRCm39)	A75E	possibly damaging	Het
Or4x13	T	A	2: 90,231,107 (GRCm39)	M34K	probably benign	Het
Or52a33	A	G	7: 103,289,308 (GRCm39)	F13S	probably damaging	Het
Or5k3	T	C	16: 58,969,981 (GRCm39)	L256P	probably damaging	Het
Or5v1	G	T	17: 37,810,017 (GRCm39)	L158F	probably benign	Het
Otud4	G	A	8: 80,382,318 (GRCm39)	V176I	probably damaging	Het
Pakap	T	A	4: 57,856,552 (GRCm39)	V668E	probably benign	Het
Papolg	T	C	11: 23,823,919 (GRCm39)		probably null	Het
Pgk2	A	G	17: 40,518,402 (GRCm39)	V342A	probably damaging	Het
Phf2	G	A	13: 48,961,320 (GRCm39)	A790V	unknown	Het
Prkdc	T	C	16: 15,491,861 (GRCm39)	I602T	possibly damaging	Het
Prl3d3	T	C	13: 27,343,072 (GRCm39)	I86T	possibly damaging	Het
Prss8	G	T	7: 127,525,635 (GRCm39)	Q295K	probably benign	Het
Ptpre	C	T	7: 135,270,861 (GRCm39)	H346Y	probably benign	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Pwwp3a	T	C	10: 80,068,702 (GRCm39)	L282P	probably benign	Het
Qrfpr	A	G	3: 36,276,285 (GRCm39)	V35A	probably damaging	Het
Rpf1	A	G	3: 146,212,293 (GRCm39)	L349S	probably damaging	Het
Rpl41	G	T	10: 128,384,652 (GRCm39)		probably null	Het
Rprd2	G	C	3: 95,672,632 (GRCm39)	R924G	probably benign	Het
Serpini1	T	A	3: 75,521,795 (GRCm39)	N95K	probably benign	Het
Sfxn1	A	T	13: 54,242,933 (GRCm39)	T64S	probably benign	Het
Siae	T	G	9: 37,557,816 (GRCm39)	I541S	possibly damaging	Het
Slc25a4	T	A	8: 46,660,509 (GRCm39)	K296N	probably benign	Het
Slc37a1	A	G	17: 31,541,120 (GRCm39)	N204S	probably damaging	Het
Slc9c1	A	G	16: 45,413,800 (GRCm39)	N976S	probably benign	Het
Smarcal1	T	C	1: 72,672,019 (GRCm39)	S847P	possibly damaging	Het
Smg1	A	T	7: 117,757,323 (GRCm39)		probably benign	Het
Smg1	C	T	7: 117,807,274 (GRCm39)	A168T	probably benign	Het
Sptlc1	A	G	13: 53,505,692 (GRCm39)	I242T	probably damaging	Het
St8sia2	T	C	7: 73,616,709 (GRCm39)	I89V	possibly damaging	Het
Supt20	A	T	3: 54,602,555 (GRCm39)		probably benign	Het
Tep1	T	C	14: 51,076,457 (GRCm39)	D1659G	probably benign	Het
Tex10	C	A	4: 48,458,525 (GRCm39)		probably benign	Het
Thbs3	CAGAAG	CAG	3: 89,130,409 (GRCm39)		probably benign	Het
Tmbim6	C	A	15: 99,299,950 (GRCm39)	S22*	probably null	Het
Tmc2	A	T	2: 130,043,961 (GRCm39)	K65M	possibly damaging	Het
Tmod1	A	G	4: 46,090,872 (GRCm39)	S142G	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trmt6	T	C	2: 132,650,191 (GRCm39)	R349G	possibly damaging	Het
Ttn	T	A	2: 76,537,586 (GRCm39)	T26454S	possibly damaging	Het
Ttn	C	T	2: 76,605,122 (GRCm39)	V16555I	probably benign	Het
Tut4	T	A	4: 108,384,042 (GRCm39)		probably benign	Het
Ubr3	T	C	2: 69,850,790 (GRCm39)		probably benign	Het
Vmn1r17	A	C	6: 57,337,460 (GRCm39)	F253V	possibly damaging	Het
Vmn1r201	G	T	13: 22,659,622 (GRCm39)	A279S	possibly damaging	Het
Vmn2r17	A	T	5: 109,575,739 (GRCm39)	R203S	probably benign	Het
Vwa5a	A	G	9: 38,633,926 (GRCm39)	E43G	probably benign	Het
Wdr86	C	T	5: 24,917,843 (GRCm39)		probably null	Het
Wdsub1	A	T	2: 59,700,758 (GRCm39)		probably benign	Het
Zcchc7	T	C	4: 44,931,039 (GRCm39)	L76P	probably damaging	Het
Zfp108	G	T	7: 23,960,163 (GRCm39)	K251N	probably benign	Het
Zfp263	T	C	16: 3,566,992 (GRCm39)	C148R	probably damaging	Het
Zfp687	A	C	3: 94,917,697 (GRCm39)	F692V	probably damaging	Het

Other mutations in Hydin

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00338:Hydin	APN	8	111,296,434 (GRCm39)	missense	possibly damaging	0.69
IGL00432:Hydin	APN	8	111,327,884 (GRCm39)	missense	probably damaging	0.98
IGL01025:Hydin	APN	8	111,053,033 (GRCm39)	missense	probably benign	0.38
IGL01140:Hydin	APN	8	111,124,694 (GRCm39)	missense	probably benign	0.14
IGL01317:Hydin	APN	8	111,053,078 (GRCm39)	missense	probably damaging	0.98
IGL01473:Hydin	APN	8	111,038,792 (GRCm39)	missense	probably benign	0.08
IGL01473:Hydin	APN	8	111,081,585 (GRCm39)	missense	probably damaging	1.00
IGL01610:Hydin	APN	8	111,284,345 (GRCm39)	missense	probably benign	0.00
IGL01685:Hydin	APN	8	111,081,665 (GRCm39)	nonsense	probably null
IGL01734:Hydin	APN	8	111,217,421 (GRCm39)	nonsense	probably null
IGL01743:Hydin	APN	8	111,319,408 (GRCm39)	missense	possibly damaging	0.94
IGL01829:Hydin	APN	8	111,316,154 (GRCm39)	missense	possibly damaging	0.68
IGL01919:Hydin	APN	8	111,245,806 (GRCm39)	missense	possibly damaging	0.89
IGL01946:Hydin	APN	8	111,217,350 (GRCm39)	missense	possibly damaging	0.91
IGL01983:Hydin	APN	8	111,241,527 (GRCm39)	missense	probably benign	0.02
IGL02122:Hydin	APN	8	111,221,047 (GRCm39)	missense	possibly damaging	0.86
IGL02140:Hydin	APN	8	111,293,570 (GRCm39)	missense	probably benign
IGL02158:Hydin	APN	8	111,336,598 (GRCm39)	missense	possibly damaging	0.89
IGL02167:Hydin	APN	8	111,145,055 (GRCm39)	missense	possibly damaging	0.96
IGL02171:Hydin	APN	8	111,178,590 (GRCm39)	nonsense	probably null
IGL02185:Hydin	APN	8	111,233,108 (GRCm39)	missense	possibly damaging	0.86
IGL02517:Hydin	APN	8	111,293,604 (GRCm39)	missense	probably benign	0.01
IGL02639:Hydin	APN	8	111,265,081 (GRCm39)	missense	probably benign	0.01
IGL02644:Hydin	APN	8	111,265,100 (GRCm39)	missense	probably damaging	1.00
IGL02652:Hydin	APN	8	111,316,154 (GRCm39)	missense	possibly damaging	0.68
IGL02658:Hydin	APN	8	111,139,908 (GRCm39)	missense	possibly damaging	0.86
IGL02706:Hydin	APN	8	111,137,198 (GRCm39)	missense	probably damaging	0.99
IGL02892:Hydin	APN	8	111,325,591 (GRCm39)	missense	possibly damaging	0.89
IGL02947:Hydin	APN	8	111,145,094 (GRCm39)	missense	probably damaging	0.96
IGL03136:Hydin	APN	8	111,145,156 (GRCm39)	missense	probably benign	0.22
IGL03248:Hydin	APN	8	111,321,921 (GRCm39)	missense	probably damaging	0.97
IGL03251:Hydin	APN	8	111,217,228 (GRCm39)	missense	probably damaging	1.00
IGL03350:Hydin	APN	8	111,038,856 (GRCm39)	missense	possibly damaging	0.86
IGL03366:Hydin	APN	8	110,993,995 (GRCm39)	missense	unknown
IGL03404:Hydin	APN	8	111,296,409 (GRCm39)	missense	probably benign	0.06
Franz_joseph	UTSW	8	111,327,950 (GRCm39)	missense	probably damaging	1.00
jahreszeiten	UTSW	8	111,295,991 (GRCm39)	missense	probably damaging	1.00
maria	UTSW	8	111,235,759 (GRCm39)	splice site	probably benign
schoepfung	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
surprise	UTSW	8	111,254,648 (GRCm39)	missense	probably benign
teresa	UTSW	8	111,336,303 (GRCm39)	missense	possibly damaging	0.79
BB001:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
BB004:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
BB011:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
BB014:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
P0005:Hydin	UTSW	8	111,220,921 (GRCm39)	critical splice acceptor site	probably null
R0099:Hydin	UTSW	8	111,316,193 (GRCm39)	missense	probably damaging	1.00
R0125:Hydin	UTSW	8	111,189,163 (GRCm39)	missense	probably benign	0.12
R0157:Hydin	UTSW	8	111,026,642 (GRCm39)	missense	possibly damaging	0.86
R0241:Hydin	UTSW	8	111,124,655 (GRCm39)	missense	probably benign	0.04
R0241:Hydin	UTSW	8	111,124,655 (GRCm39)	missense	probably benign	0.04
R0255:Hydin	UTSW	8	111,291,650 (GRCm39)	missense	probably benign	0.00
R0352:Hydin	UTSW	8	111,296,533 (GRCm39)	critical splice donor site	probably null
R0379:Hydin	UTSW	8	111,235,759 (GRCm39)	splice site	probably benign
R0468:Hydin	UTSW	8	111,139,855 (GRCm39)	missense	possibly damaging	0.96
R0477:Hydin	UTSW	8	111,145,130 (GRCm39)	missense	probably damaging	1.00
R0479:Hydin	UTSW	8	111,325,720 (GRCm39)	missense	probably damaging	1.00
R0539:Hydin	UTSW	8	111,249,704 (GRCm39)	missense	probably benign
R0550:Hydin	UTSW	8	111,314,407 (GRCm39)	missense	probably benign	0.01
R0571:Hydin	UTSW	8	111,240,735 (GRCm39)	splice site	probably null
R0606:Hydin	UTSW	8	111,276,430 (GRCm39)	splice site	probably benign
R0789:Hydin	UTSW	8	111,293,603 (GRCm39)	missense	possibly damaging	0.53
R0849:Hydin	UTSW	8	111,325,616 (GRCm39)	missense	probably damaging	1.00
R0946:Hydin	UTSW	8	111,257,685 (GRCm39)	missense	probably benign	0.25
R1201:Hydin	UTSW	8	111,296,487 (GRCm39)	missense	probably benign	0.01
R1375:Hydin	UTSW	8	111,232,854 (GRCm39)	critical splice donor site	probably null
R1385:Hydin	UTSW	8	111,249,836 (GRCm39)	missense	probably benign	0.40
R1411:Hydin	UTSW	8	111,301,663 (GRCm39)	missense	probably benign	0.04
R1437:Hydin	UTSW	8	111,308,617 (GRCm39)	nonsense	probably null
R1447:Hydin	UTSW	8	111,249,798 (GRCm39)	missense	probably damaging	1.00
R1448:Hydin	UTSW	8	111,173,217 (GRCm39)	missense	probably benign	0.27
R1466:Hydin	UTSW	8	111,259,585 (GRCm39)	missense	possibly damaging	0.47
R1466:Hydin	UTSW	8	111,259,585 (GRCm39)	missense	possibly damaging	0.47
R1523:Hydin	UTSW	8	111,259,903 (GRCm39)	missense	probably benign	0.05
R1544:Hydin	UTSW	8	111,301,486 (GRCm39)	missense	probably benign	0.30
R1581:Hydin	UTSW	8	111,137,092 (GRCm39)	missense	probably benign
R1584:Hydin	UTSW	8	111,307,447 (GRCm39)	missense	probably benign	0.27
R1598:Hydin	UTSW	8	111,137,306 (GRCm39)	missense	possibly damaging	0.96
R1633:Hydin	UTSW	8	111,233,614 (GRCm39)	missense	probably benign	0.10
R1777:Hydin	UTSW	8	111,316,203 (GRCm39)	missense	probably benign	0.14
R1817:Hydin	UTSW	8	111,259,459 (GRCm39)	missense	probably benign	0.00
R1828:Hydin	UTSW	8	111,237,526 (GRCm39)	missense	probably benign	0.03
R1837:Hydin	UTSW	8	111,296,257 (GRCm39)	missense	probably benign	0.20
R1848:Hydin	UTSW	8	111,296,440 (GRCm39)	missense	probably benign	0.19
R1869:Hydin	UTSW	8	111,227,337 (GRCm39)	missense	possibly damaging	0.94
R1909:Hydin	UTSW	8	111,314,404 (GRCm39)	missense	probably damaging	1.00
R1928:Hydin	UTSW	8	111,229,579 (GRCm39)	missense	possibly damaging	0.93
R1950:Hydin	UTSW	8	111,336,619 (GRCm39)	missense	possibly damaging	0.64
R2095:Hydin	UTSW	8	111,189,289 (GRCm39)	missense	probably damaging	0.96
R2172:Hydin	UTSW	8	111,308,681 (GRCm39)	missense	probably benign	0.42
R2217:Hydin	UTSW	8	111,145,138 (GRCm39)	missense	probably benign
R2248:Hydin	UTSW	8	111,304,835 (GRCm39)	missense	probably benign	0.09
R2272:Hydin	UTSW	8	111,035,764 (GRCm39)	missense	probably benign	0.01
R2294:Hydin	UTSW	8	111,026,591 (GRCm39)	missense	probably damaging	0.99
R2315:Hydin	UTSW	8	111,124,676 (GRCm39)	missense	probably benign	0.01
R2330:Hydin	UTSW	8	111,291,641 (GRCm39)	missense	probably benign	0.01
R2374:Hydin	UTSW	8	111,291,780 (GRCm39)	missense	probably damaging	1.00
R2446:Hydin	UTSW	8	111,314,347 (GRCm39)	missense	possibly damaging	0.82
R2484:Hydin	UTSW	8	111,239,747 (GRCm39)	missense	possibly damaging	0.76
R2698:Hydin	UTSW	8	111,336,561 (GRCm39)	missense	possibly damaging	0.70
R2843:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2844:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2846:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2882:Hydin	UTSW	8	111,293,555 (GRCm39)	missense	possibly damaging	0.92
R2937:Hydin	UTSW	8	111,130,927 (GRCm39)	missense	possibly damaging	0.88
R3031:Hydin	UTSW	8	111,329,848 (GRCm39)	missense	possibly damaging	0.83
R3038:Hydin	UTSW	8	111,309,321 (GRCm39)	missense	probably damaging	1.00
R3121:Hydin	UTSW	8	111,233,138 (GRCm39)	missense	probably benign
R3157:Hydin	UTSW	8	110,994,005 (GRCm39)	missense	unknown
R3547:Hydin	UTSW	8	111,308,699 (GRCm39)	missense	possibly damaging	0.85
R3696:Hydin	UTSW	8	111,329,911 (GRCm39)	missense	probably damaging	1.00
R3850:Hydin	UTSW	8	111,290,561 (GRCm39)	missense	probably damaging	0.99
R3896:Hydin	UTSW	8	111,235,711 (GRCm39)	missense	possibly damaging	0.93
R3983:Hydin	UTSW	8	111,118,957 (GRCm39)	missense	probably damaging	1.00
R4031:Hydin	UTSW	8	111,336,679 (GRCm39)	missense	probably benign	0.30
R4072:Hydin	UTSW	8	111,231,888 (GRCm39)	missense	possibly damaging	0.68
R4095:Hydin	UTSW	8	111,268,179 (GRCm39)	missense	probably damaging	0.98
R4176:Hydin	UTSW	8	111,320,452 (GRCm39)	missense	probably benign	0.00
R4213:Hydin	UTSW	8	111,183,139 (GRCm39)	missense	possibly damaging	0.91
R4412:Hydin	UTSW	8	111,142,368 (GRCm39)	missense	probably damaging	0.99
R4471:Hydin	UTSW	8	111,313,764 (GRCm39)	missense	probably damaging	1.00
R4474:Hydin	UTSW	8	111,290,497 (GRCm39)	missense	probably benign	0.11
R4495:Hydin	UTSW	8	111,322,034 (GRCm39)	missense	probably damaging	0.99
R4508:Hydin	UTSW	8	111,245,886 (GRCm39)	missense	possibly damaging	0.91
R4578:Hydin	UTSW	8	110,993,971 (GRCm39)	missense	unknown
R4583:Hydin	UTSW	8	111,321,857 (GRCm39)	missense	probably benign	0.36
R4600:Hydin	UTSW	8	111,293,582 (GRCm39)	missense	probably benign	0.04
R4681:Hydin	UTSW	8	111,233,103 (GRCm39)	missense	possibly damaging	0.85
R4685:Hydin	UTSW	8	111,189,154 (GRCm39)	missense	probably damaging	0.99
R4689:Hydin	UTSW	8	111,322,046 (GRCm39)	missense	probably benign	0.18
R4735:Hydin	UTSW	8	111,282,264 (GRCm39)	critical splice donor site	probably null
R4736:Hydin	UTSW	8	111,249,840 (GRCm39)	missense	probably benign	0.02
R4740:Hydin	UTSW	8	111,173,071 (GRCm39)	missense	probably benign	0.06
R4771:Hydin	UTSW	8	111,259,515 (GRCm39)	missense	probably benign
R4777:Hydin	UTSW	8	111,137,096 (GRCm39)	missense	probably damaging	0.98
R4859:Hydin	UTSW	8	111,233,126 (GRCm39)	missense	possibly damaging	0.93
R4911:Hydin	UTSW	8	111,322,070 (GRCm39)	missense	probably benign	0.01
R4964:Hydin	UTSW	8	111,217,305 (GRCm39)	missense	possibly damaging	0.86
R4965:Hydin	UTSW	8	111,124,727 (GRCm39)	missense	probably benign
R4995:Hydin	UTSW	8	111,296,274 (GRCm39)	missense	probably damaging	0.97
R5059:Hydin	UTSW	8	111,232,401 (GRCm39)	missense	probably damaging	0.96
R5071:Hydin	UTSW	8	111,265,105 (GRCm39)	missense	probably benign	0.03
R5073:Hydin	UTSW	8	111,265,105 (GRCm39)	missense	probably benign	0.03
R5092:Hydin	UTSW	8	111,309,300 (GRCm39)	missense	probably benign	0.16
R5156:Hydin	UTSW	8	111,336,333 (GRCm39)	missense	probably benign	0.00
R5166:Hydin	UTSW	8	111,249,774 (GRCm39)	missense	possibly damaging	0.89
R5189:Hydin	UTSW	8	111,139,843 (GRCm39)	critical splice acceptor site	probably null
R5243:Hydin	UTSW	8	111,232,380 (GRCm39)	missense	possibly damaging	0.92
R5244:Hydin	UTSW	8	111,259,451 (GRCm39)	missense	possibly damaging	0.77
R5256:Hydin	UTSW	8	111,313,855 (GRCm39)	missense	possibly damaging	0.92
R5266:Hydin	UTSW	8	111,061,416 (GRCm39)	missense	possibly damaging	0.87
R5283:Hydin	UTSW	8	111,178,612 (GRCm39)	missense	possibly damaging	0.96
R5343:Hydin	UTSW	8	111,212,051 (GRCm39)	missense	probably benign	0.40
R5359:Hydin	UTSW	8	111,265,004 (GRCm39)	missense	probably benign	0.00
R5390:Hydin	UTSW	8	111,322,099 (GRCm39)	missense	probably benign
R5394:Hydin	UTSW	8	111,266,474 (GRCm39)	splice site	probably null
R5441:Hydin	UTSW	8	111,291,741 (GRCm39)	missense	possibly damaging	0.72
R5461:Hydin	UTSW	8	111,245,863 (GRCm39)	missense	probably damaging	0.96
R5662:Hydin	UTSW	8	111,307,341 (GRCm39)	missense	probably benign	0.02
R5695:Hydin	UTSW	8	111,261,915 (GRCm39)	missense	probably benign	0.35
R5732:Hydin	UTSW	8	111,178,690 (GRCm39)	missense	probably benign	0.03
R5774:Hydin	UTSW	8	111,298,547 (GRCm39)	nonsense	probably null
R5780:Hydin	UTSW	8	111,312,712 (GRCm39)	missense	probably damaging	1.00
R5787:Hydin	UTSW	8	111,052,985 (GRCm39)	missense	probably damaging	0.99
R5802:Hydin	UTSW	8	111,178,692 (GRCm39)	missense	possibly damaging	0.86
R5841:Hydin	UTSW	8	111,259,846 (GRCm39)	missense	possibly damaging	0.76
R5856:Hydin	UTSW	8	111,268,474 (GRCm39)	missense	probably damaging	0.99
R5893:Hydin	UTSW	8	111,217,308 (GRCm39)	missense	probably benign	0.12
R5963:Hydin	UTSW	8	111,220,926 (GRCm39)	missense	possibly damaging	0.93
R6008:Hydin	UTSW	8	111,325,717 (GRCm39)	missense	probably benign	0.02
R6019:Hydin	UTSW	8	111,293,252 (GRCm39)	missense	probably benign
R6038:Hydin	UTSW	8	111,325,663 (GRCm39)	missense	probably benign	0.16
R6038:Hydin	UTSW	8	111,325,663 (GRCm39)	missense	probably benign	0.16
R6133:Hydin	UTSW	8	111,327,908 (GRCm39)	missense	probably benign	0.00
R6135:Hydin	UTSW	8	111,189,292 (GRCm39)	missense	possibly damaging	0.85
R6157:Hydin	UTSW	8	111,254,648 (GRCm39)	missense	probably benign
R6209:Hydin	UTSW	8	111,320,434 (GRCm39)	missense	probably benign	0.05
R6238:Hydin	UTSW	8	111,118,743 (GRCm39)	splice site	probably null
R6293:Hydin	UTSW	8	111,324,543 (GRCm39)	missense	possibly damaging	0.83
R6340:Hydin	UTSW	8	111,081,574 (GRCm39)	splice site	probably null
R6349:Hydin	UTSW	8	111,145,091 (GRCm39)	nonsense	probably null
R6357:Hydin	UTSW	8	111,268,289 (GRCm39)	missense	possibly damaging	0.86
R6385:Hydin	UTSW	8	111,038,856 (GRCm39)	missense	possibly damaging	0.86
R6396:Hydin	UTSW	8	111,233,521 (GRCm39)	missense	probably damaging	0.96
R6466:Hydin	UTSW	8	111,233,600 (GRCm39)	missense	possibly damaging	0.85
R6648:Hydin	UTSW	8	111,252,299 (GRCm39)	splice site	probably null
R6671:Hydin	UTSW	8	111,327,950 (GRCm39)	missense	probably damaging	1.00
R6695:Hydin	UTSW	8	111,053,092 (GRCm39)	missense	probably benign	0.05
R6800:Hydin	UTSW	8	111,324,603 (GRCm39)	missense	probably benign	0.09
R6841:Hydin	UTSW	8	111,265,007 (GRCm39)	missense	probably benign	0.09
R6867:Hydin	UTSW	8	111,266,434 (GRCm39)	missense	probably benign	0.08
R6889:Hydin	UTSW	8	111,259,488 (GRCm39)	missense	possibly damaging	0.79
R6895:Hydin	UTSW	8	111,038,883 (GRCm39)	missense	probably benign	0.00
R6940:Hydin	UTSW	8	111,217,243 (GRCm39)	missense	probably damaging	1.00
R6951:Hydin	UTSW	8	111,124,757 (GRCm39)	missense	probably benign
R6980:Hydin	UTSW	8	111,139,916 (GRCm39)	missense	possibly damaging	0.91
R6981:Hydin	UTSW	8	111,257,704 (GRCm39)	missense	possibly damaging	0.89
R7061:Hydin	UTSW	8	111,329,920 (GRCm39)	missense	possibly damaging	0.90
R7085:Hydin	UTSW	8	111,329,962 (GRCm39)	missense	probably benign	0.03
R7086:Hydin	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
R7110:Hydin	UTSW	8	111,081,583 (GRCm39)	critical splice acceptor site	probably null
R7158:Hydin	UTSW	8	111,336,303 (GRCm39)	missense	possibly damaging	0.79
R7163:Hydin	UTSW	8	111,329,968 (GRCm39)	missense	probably benign	0.25
R7209:Hydin	UTSW	8	111,216,424 (GRCm39)	nonsense	probably null
R7244:Hydin	UTSW	8	111,276,307 (GRCm39)	missense	probably damaging	0.98
R7347:Hydin	UTSW	8	111,326,994 (GRCm39)	missense	probably benign	0.06
R7349:Hydin	UTSW	8	111,124,803 (GRCm39)	splice site	probably null
R7359:Hydin	UTSW	8	111,232,733 (GRCm39)	missense	probably damaging	0.98
R7365:Hydin	UTSW	8	111,327,905 (GRCm39)	missense	probably damaging	1.00
R7365:Hydin	UTSW	8	111,284,294 (GRCm39)	missense	probably damaging	0.99
R7436:Hydin	UTSW	8	111,310,546 (GRCm39)	missense	probably damaging	0.96
R7528:Hydin	UTSW	8	111,107,204 (GRCm39)	nonsense	probably null
R7544:Hydin	UTSW	8	111,316,157 (GRCm39)	missense	probably benign	0.35
R7625:Hydin	UTSW	8	111,268,476 (GRCm39)	missense	probably benign	0.01
R7713:Hydin	UTSW	8	111,320,444 (GRCm39)	missense	possibly damaging	0.69
R7763:Hydin	UTSW	8	111,232,475 (GRCm39)	missense	possibly damaging	0.92
R7771:Hydin	UTSW	8	111,291,717 (GRCm39)	missense	probably damaging	0.97
R7794:Hydin	UTSW	8	111,235,715 (GRCm39)	missense	probably damaging	1.00
R7833:Hydin	UTSW	8	111,316,092 (GRCm39)	missense	probably damaging	0.99
R7894:Hydin	UTSW	8	111,239,642 (GRCm39)	missense	possibly damaging	0.88
R7899:Hydin	UTSW	8	111,314,380 (GRCm39)	missense	probably benign	0.00
R7908:Hydin	UTSW	8	111,237,499 (GRCm39)	missense	probably benign	0.01
R7912:Hydin	UTSW	8	111,282,239 (GRCm39)	missense	possibly damaging	0.68
R7919:Hydin	UTSW	8	110,993,971 (GRCm39)	missense	unknown
R7924:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
R7927:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
R7970:Hydin	UTSW	8	111,035,723 (GRCm39)	missense	probably damaging	0.99
R7993:Hydin	UTSW	8	111,306,264 (GRCm39)	missense	probably benign
R8011:Hydin	UTSW	8	111,310,541 (GRCm39)	missense	probably damaging	1.00
R8041:Hydin	UTSW	8	111,301,626 (GRCm39)	missense	probably benign	0.02
R8080:Hydin	UTSW	8	111,261,863 (GRCm39)	missense	probably benign	0.32
R8081:Hydin	UTSW	8	111,092,101 (GRCm39)	missense	possibly damaging	0.93
R8095:Hydin	UTSW	8	111,295,991 (GRCm39)	missense	probably damaging	1.00
R8157:Hydin	UTSW	8	111,178,668 (GRCm39)	missense	probably benign	0.33
R8186:Hydin	UTSW	8	111,336,277 (GRCm39)	missense	probably benign	0.14
R8205:Hydin	UTSW	8	111,319,270 (GRCm39)	missense	possibly damaging	0.57
R8263:Hydin	UTSW	8	111,178,705 (GRCm39)	missense	probably benign	0.00
R8288:Hydin	UTSW	8	111,233,661 (GRCm39)	missense	probably damaging	0.96
R8298:Hydin	UTSW	8	111,327,015 (GRCm39)	missense	probably damaging	1.00
R8309:Hydin	UTSW	8	111,334,534 (GRCm39)	missense	probably benign	0.18
R8348:Hydin	UTSW	8	111,329,878 (GRCm39)	missense	possibly damaging	0.68
R8356:Hydin	UTSW	8	111,259,756 (GRCm39)	missense	possibly damaging	0.67
R8406:Hydin	UTSW	8	111,336,543 (GRCm39)	missense	possibly damaging	0.94
R8415:Hydin	UTSW	8	111,178,626 (GRCm39)	missense	probably damaging	1.00
R8417:Hydin	UTSW	8	111,296,024 (GRCm39)	missense	probably benign	0.28
R8432:Hydin	UTSW	8	111,324,583 (GRCm39)	missense	probably benign	0.02
R8437:Hydin	UTSW	8	111,189,367 (GRCm39)	missense	probably damaging	0.96
R8463:Hydin	UTSW	8	111,237,553 (GRCm39)	missense	probably benign	0.22
R8508:Hydin	UTSW	8	111,308,650 (GRCm39)	missense	probably benign	0.00
R8510:Hydin	UTSW	8	111,233,202 (GRCm39)	missense	probably damaging	1.00
R8560:Hydin	UTSW	8	111,265,106 (GRCm39)	missense	probably benign	0.09
R8682:Hydin	UTSW	8	111,035,798 (GRCm39)	missense	probably damaging	0.96
R8697:Hydin	UTSW	8	111,259,515 (GRCm39)	missense	probably benign
R8857:Hydin	UTSW	8	111,298,587 (GRCm39)	critical splice donor site	probably null
R8866:Hydin	UTSW	8	111,308,779 (GRCm39)	missense	possibly damaging	0.89
R8878:Hydin	UTSW	8	111,035,720 (GRCm39)	missense	probably benign	0.12
R8897:Hydin	UTSW	8	111,316,112 (GRCm39)	missense	probably benign
R8987:Hydin	UTSW	8	111,239,766 (GRCm39)	nonsense	probably null
R9072:Hydin	UTSW	8	110,994,083 (GRCm39)	critical splice donor site	probably null
R9073:Hydin	UTSW	8	110,994,083 (GRCm39)	critical splice donor site	probably null
R9102:Hydin	UTSW	8	111,235,546 (GRCm39)	missense	probably benign	0.33
R9224:Hydin	UTSW	8	111,259,516 (GRCm39)	missense	probably benign
R9255:Hydin	UTSW	8	111,261,972 (GRCm39)	missense	probably benign	0.23
R9257:Hydin	UTSW	8	111,301,648 (GRCm39)	missense	probably damaging	0.99
R9261:Hydin	UTSW	8	110,994,047 (GRCm39)	missense	unknown
R9273:Hydin	UTSW	8	111,233,580 (GRCm39)	missense	probably damaging	0.98
R9376:Hydin	UTSW	8	111,124,695 (GRCm39)	missense	possibly damaging	0.70
R9380:Hydin	UTSW	8	111,290,504 (GRCm39)	missense	probably benign	0.07
R9386:Hydin	UTSW	8	111,314,362 (GRCm39)	missense	probably benign
R9406:Hydin	UTSW	8	111,314,412 (GRCm39)	missense	probably null	0.96
R9492:Hydin	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
R9513:Hydin	UTSW	8	111,322,114 (GRCm39)	missense	probably damaging	0.99
R9562:Hydin	UTSW	8	111,312,786 (GRCm39)	missense	probably benign	0.11
R9664:Hydin	UTSW	8	111,220,965 (GRCm39)	missense	probably benign	0.01
R9733:Hydin	UTSW	8	111,262,011 (GRCm39)	missense	probably benign
R9753:Hydin	UTSW	8	111,217,398 (GRCm39)	missense	possibly damaging	0.85
X0063:Hydin	UTSW	8	111,277,951 (GRCm39)	missense	probably damaging	1.00
Z1088:Hydin	UTSW	8	111,319,423 (GRCm39)	frame shift	probably null
Z1088:Hydin	UTSW	8	111,312,680 (GRCm39)	missense	probably benign	0.00
Z1088:Hydin	UTSW	8	111,026,605 (GRCm39)	missense	probably benign	0.12
Z1176:Hydin	UTSW	8	111,268,232 (GRCm39)	missense	possibly damaging	0.65
Z1177:Hydin	UTSW	8	111,313,774 (GRCm39)	frame shift	probably null
Z1177:Hydin	UTSW	8	111,176,864 (GRCm39)	missense	possibly damaging	0.47
Z1177:Hydin	UTSW	8	111,107,242 (GRCm39)	missense	probably damaging	1.00
Z1177:Hydin	UTSW	8	111,336,621 (GRCm39)	missense	probably benign	0.10
Z1188:Hydin	UTSW	8	111,142,419 (GRCm39)	missense	probably benign	0.40

Predicted Primers

PCR Primer
(F):5'- GCTCCTTTGGAAAGTCTCCTAC -3'
(R):5'- AAGGTTCCAGATGAAGTGACCC -3'

Sequencing Primer
(F):5'- GGAAAGTCTCCTACTCTGTTACC -3'
(R):5'- AGATGAAGTGACCCCTCTGTGTC -3'

Posted On

2016-05-10