Incidental Mutation 'R5062:Klhl30'
ID 386659
Institutional Source Beutler Lab
Gene Symbol Klhl30
Ensembl Gene ENSMUSG00000026308
Gene Name kelch-like 30
Synonyms 4631423F02Rik
MMRRC Submission 042652-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5062 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 91278795-91290126 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 91283300 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 301 (T301A)
Ref Sequence ENSEMBL: ENSMUSP00000027533 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027533]
AlphaFold Q8C3F7
Predicted Effect probably benign
Transcript: ENSMUST00000027533
AA Change: T301A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000027533
Gene: ENSMUSG00000026308
AA Change: T301A

DomainStartEndE-ValueType
BTB 33 130 1.41e-24 SMART
BACK 135 237 5.11e-26 SMART
Kelch 328 378 2.2e-5 SMART
Kelch 379 423 7.4e-2 SMART
Kelch 473 514 1e1 SMART
Kelch 515 564 2.7e0 SMART
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 95.7%
  • 20x: 90.1%
Validation Efficiency 100% (79/79)
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca6 A T 11: 110,067,892 (GRCm39) I1593N probably benign Het
Akr1b10 G T 6: 34,369,041 (GRCm39) K173N probably damaging Het
Artn A T 4: 117,784,873 (GRCm39) L3Q probably damaging Het
Asxl3 T A 18: 22,655,775 (GRCm39) S1262T possibly damaging Het
Atp6v0a4 A T 6: 38,051,118 (GRCm39) M420K probably benign Het
Bcam T A 7: 19,494,026 (GRCm39) T422S possibly damaging Het
Casp2 C T 6: 42,246,206 (GRCm39) probably benign Het
Ccdc137 A G 11: 120,353,341 (GRCm39) probably benign Het
Cd177 C A 7: 24,443,741 (GRCm39) A786S probably benign Het
Cdca4 T C 12: 112,785,483 (GRCm39) N82D probably benign Het
Clu A C 14: 66,217,177 (GRCm39) T337P probably damaging Het
Col6a3 A C 1: 90,707,074 (GRCm39) I2013S unknown Het
Cpne9 T A 6: 113,281,449 (GRCm39) M510K probably damaging Het
Cyp3a13 A C 5: 137,897,161 (GRCm39) N384K possibly damaging Het
Fam186a A C 15: 99,842,527 (GRCm39) I1239S possibly damaging Het
Fryl T C 5: 73,233,236 (GRCm39) E413G possibly damaging Het
Fscn2 A C 11: 120,257,575 (GRCm39) Y312S probably damaging Het
Fshr A T 17: 89,293,474 (GRCm39) C401* probably null Het
Glyat A C 19: 12,627,627 (GRCm39) Q74P probably damaging Het
Gm6158 A T 14: 24,120,158 (GRCm39) noncoding transcript Het
Grm7 A G 6: 110,623,097 (GRCm39) N90S probably damaging Het
Hectd1 A T 12: 51,791,662 (GRCm39) C2536S probably damaging Het
Herc3 C T 6: 58,832,745 (GRCm39) Q137* probably null Het
Kctd3 A C 1: 188,727,890 (GRCm39) probably benign Het
Klra9 T C 6: 130,156,072 (GRCm39) K228E possibly damaging Het
Lamc3 A T 2: 31,795,679 (GRCm39) T355S possibly damaging Het
Lcmt1 A C 7: 123,010,053 (GRCm39) probably null Het
Limch1 C T 5: 67,126,578 (GRCm39) P60S probably damaging Het
Lrfn2 A G 17: 49,377,528 (GRCm39) D203G probably damaging Het
Mc5r T C 18: 68,472,352 (GRCm39) L237P probably damaging Het
Mknk2 G A 10: 80,507,603 (GRCm39) R58W probably damaging Het
Mrgpra2b T C 7: 47,152,676 (GRCm39) probably benign Het
Mroh2a GCCC GC 1: 88,159,979 (GRCm39) probably null Het
Nae1 A T 8: 105,243,334 (GRCm39) C395S possibly damaging Het
Ncoa1 A G 12: 4,309,333 (GRCm39) M1321T probably damaging Het
Neb A C 2: 52,170,513 (GRCm39) F1720V possibly damaging Het
Nectin2 C T 7: 19,472,198 (GRCm39) V64I probably benign Het
Nisch T A 14: 30,894,397 (GRCm39) T1145S probably damaging Het
Nlrp5 A T 7: 23,135,335 (GRCm39) R1009* probably null Het
Or12d13 A T 17: 37,647,822 (GRCm39) H100Q probably damaging Het
Or4k15 T A 14: 50,364,894 (GRCm39) Y287N probably damaging Het
Otx1 C A 11: 21,947,037 (GRCm39) A91S probably damaging Het
Pak1ip1 A G 13: 41,161,621 (GRCm39) probably benign Het
Pcm1 T C 8: 41,712,297 (GRCm39) V189A probably damaging Het
Peak1 G T 9: 56,167,573 (GRCm39) N118K probably damaging Het
Phgdh T A 3: 98,235,655 (GRCm39) I121F probably damaging Het
Pi4ka G T 16: 17,127,261 (GRCm39) A1064E probably benign Het
Pkhd1 A C 1: 20,655,935 (GRCm39) C199W probably benign Het
Plat A G 8: 23,262,327 (GRCm39) D117G probably benign Het
Ppp2r2b C A 18: 42,821,526 (GRCm39) V211L possibly damaging Het
Ptgs1 A G 2: 36,127,294 (GRCm39) D60G probably damaging Het
Ryr2 T C 13: 11,715,240 (GRCm39) E2776G probably damaging Het
Sema4c C T 1: 36,592,059 (GRCm39) probably null Het
Sharpin T C 15: 76,231,811 (GRCm39) probably benign Het
Slamf6 A G 1: 171,764,100 (GRCm39) I164M possibly damaging Het
Slco1a7 A C 6: 141,713,180 (GRCm39) M67R possibly damaging Het
Spef1 A G 2: 131,015,201 (GRCm39) Y46H probably damaging Het
Spns1 G A 7: 125,973,501 (GRCm39) probably benign Het
Supt5 C T 7: 28,028,440 (GRCm39) probably null Het
Tbx5 T A 5: 119,974,987 (GRCm39) D3E probably damaging Het
Thbs1 T C 2: 117,951,718 (GRCm39) probably null Het
Tmem140 G A 6: 34,849,897 (GRCm39) V138M probably damaging Het
Tmem200a T A 10: 25,869,813 (GRCm39) D152V probably damaging Het
Tmem200b A G 4: 131,649,848 (GRCm39) D256G probably damaging Het
Tns1 A T 1: 73,992,023 (GRCm39) L885Q probably damaging Het
Umod G A 7: 119,071,644 (GRCm39) Q366* probably null Het
Vsir A G 10: 60,200,042 (GRCm39) I208V probably damaging Het
Zfp646 G A 7: 127,479,671 (GRCm39) R616H probably damaging Het
Other mutations in Klhl30
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00229:Klhl30 APN 1 91,281,879 (GRCm39) missense possibly damaging 0.79
IGL01485:Klhl30 APN 1 91,281,761 (GRCm39) missense probably damaging 0.98
IGL02698:Klhl30 APN 1 91,281,429 (GRCm39) missense probably damaging 1.00
IGL02751:Klhl30 APN 1 91,281,821 (GRCm39) missense probably damaging 1.00
BB006:Klhl30 UTSW 1 91,283,238 (GRCm39) missense possibly damaging 0.67
BB016:Klhl30 UTSW 1 91,283,238 (GRCm39) missense possibly damaging 0.67
R0458:Klhl30 UTSW 1 91,288,718 (GRCm39) splice site probably benign
R0578:Klhl30 UTSW 1 91,282,074 (GRCm39) missense probably benign 0.00
R0621:Klhl30 UTSW 1 91,285,585 (GRCm39) missense probably damaging 1.00
R0645:Klhl30 UTSW 1 91,283,228 (GRCm39) missense probably damaging 0.98
R1240:Klhl30 UTSW 1 91,288,737 (GRCm39) missense probably benign 0.00
R1374:Klhl30 UTSW 1 91,288,798 (GRCm39) missense probably damaging 1.00
R2029:Klhl30 UTSW 1 91,285,636 (GRCm39) splice site probably null
R2126:Klhl30 UTSW 1 91,286,499 (GRCm39) splice site probably null
R2152:Klhl30 UTSW 1 91,285,546 (GRCm39) missense probably benign 0.32
R3913:Klhl30 UTSW 1 91,287,166 (GRCm39) missense possibly damaging 0.92
R4031:Klhl30 UTSW 1 91,288,879 (GRCm39) missense probably benign 0.01
R4116:Klhl30 UTSW 1 91,281,830 (GRCm39) missense probably benign 0.20
R4427:Klhl30 UTSW 1 91,281,426 (GRCm39) missense probably damaging 1.00
R4561:Klhl30 UTSW 1 91,288,753 (GRCm39) missense probably damaging 1.00
R4896:Klhl30 UTSW 1 91,287,046 (GRCm39) splice site probably null
R4961:Klhl30 UTSW 1 91,285,106 (GRCm39) missense possibly damaging 0.82
R5004:Klhl30 UTSW 1 91,287,046 (GRCm39) splice site probably null
R6298:Klhl30 UTSW 1 91,285,086 (GRCm39) missense probably benign 0.24
R6299:Klhl30 UTSW 1 91,285,636 (GRCm39) splice site probably null
R6393:Klhl30 UTSW 1 91,288,912 (GRCm39) missense probably damaging 1.00
R6962:Klhl30 UTSW 1 91,285,137 (GRCm39) missense probably damaging 0.99
R7461:Klhl30 UTSW 1 91,285,130 (GRCm39) missense possibly damaging 0.90
R7849:Klhl30 UTSW 1 91,287,059 (GRCm39) missense probably benign 0.00
R7929:Klhl30 UTSW 1 91,283,238 (GRCm39) missense possibly damaging 0.67
R8378:Klhl30 UTSW 1 91,285,494 (GRCm39) nonsense probably null
R8944:Klhl30 UTSW 1 91,287,174 (GRCm39) missense probably damaging 1.00
R9790:Klhl30 UTSW 1 91,282,089 (GRCm39) missense probably benign 0.23
R9791:Klhl30 UTSW 1 91,282,089 (GRCm39) missense probably benign 0.23
Z1176:Klhl30 UTSW 1 91,287,187 (GRCm39) missense probably damaging 0.96
Predicted Primers PCR Primer
(F):5'- GTGTGCACACATACCAAAGC -3'
(R):5'- TGGACTGACAGTATTCCCACC -3'

Sequencing Primer
(F):5'- TACACGTGACCCTCAGGCTAG -3'
(R):5'- GACAGTATTCCCACCCTCCTC -3'
Posted On 2016-06-06