Mutagenetix > Incidental Mutation

Incidental Mutation 'R5018:Vmn2r91'

389071

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r91

Ensembl Gene

ENSMUSG00000091206

Gene Name

vomeronasal 2, receptor 91

Synonyms

EG665210

MMRRC Submission

042609-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R5018 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18305319-18356905 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 18356700 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Phenylalanine at position 789 (C789F)

Ref Sequence

ENSEMBL: ENSMUSP00000127465 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172359]

AlphaFold

E9Q2U5

Predicted Effect

probably damaging
Transcript: ENSMUST00000172359
AA Change: C789F

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000127465
Gene: ENSMUSG00000091206
AA Change: C789F

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	83	462	2.2e-38	PFAM
Pfam:NCD3G	510	564	6.7e-20	PFAM
Pfam:7tm_3	597	832	2.1e-53	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.8%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamdec1	A	T	14: 68,809,228 (GRCm39)	F245I	probably damaging	Het
Agbl5	G	A	5: 31,060,403 (GRCm39)	R141Q	probably damaging	Het
Alx4	G	T	2: 93,507,764 (GRCm39)	G353V	probably damaging	Het
Apol7b	A	G	15: 77,308,916 (GRCm39)	F61L	probably benign	Het
Aunip	T	A	4: 134,250,928 (GRCm39)		probably null	Het
Bfsp1	C	A	2: 143,704,802 (GRCm39)	R17L	possibly damaging	Het
Birc6	T	A	17: 74,947,054 (GRCm39)	D2926E	probably damaging	Het
Dmwd	A	G	7: 18,812,044 (GRCm39)	D166G	probably damaging	Het
Dnah10	T	C	5: 124,839,260 (GRCm39)	S1233P	possibly damaging	Het
Dnah11	G	T	12: 118,094,463 (GRCm39)	N868K	probably benign	Het
Eea1	T	C	10: 95,846,899 (GRCm39)	V393A	probably benign	Het
Fchsd1	C	T	18: 38,092,926 (GRCm39)		probably benign	Het
Flacc1	A	G	1: 58,730,109 (GRCm39)	V67A	probably benign	Het
Fyttd1	A	G	16: 32,722,787 (GRCm39)		probably null	Het
Hal	T	C	10: 93,343,413 (GRCm39)		probably null	Het
Hhat	A	T	1: 192,277,346 (GRCm39)	L371Q	probably damaging	Het
Hpse2	A	G	19: 43,373,263 (GRCm39)	F122S	possibly damaging	Het
Kif21b	A	G	1: 136,099,972 (GRCm39)	I1509V	probably benign	Het
Klhl20	T	C	1: 160,929,156 (GRCm39)	D334G	probably damaging	Het
Macf1	C	T	4: 123,279,392 (GRCm39)	D3870N	probably damaging	Het
Nlrc5	C	T	8: 95,252,080 (GRCm39)	A1867V	probably damaging	Het
Nr1h5	A	G	3: 102,855,111 (GRCm39)	L330P	probably damaging	Het
Or10g3b	A	C	14: 52,586,736 (GRCm39)	C256G	possibly damaging	Het
Pcdh15	T	G	10: 74,479,607 (GRCm39)	S573A	possibly damaging	Het
Polr1c	G	T	17: 46,558,635 (GRCm39)		probably benign	Het
Scd4	T	A	19: 44,326,048 (GRCm39)	M134K	probably benign	Het
Sh3gl2	A	G	4: 85,309,291 (GRCm39)		probably benign	Het
Sin3a	T	A	9: 57,018,175 (GRCm39)	S865T	probably benign	Het
Slitrk3	G	A	3: 72,957,845 (GRCm39)	T309I	probably benign	Het
Sspo	G	A	6: 48,432,634 (GRCm39)	E837K	probably damaging	Het
Stag1	A	G	9: 100,833,672 (GRCm39)	D1095G	probably benign	Het
Trat1	A	T	16: 48,555,168 (GRCm39)	L188*	probably null	Het
Ubn1	A	G	16: 4,881,589 (GRCm39)	D207G	probably damaging	Het
Ugp2	A	G	11: 21,281,052 (GRCm39)	Y219H	probably damaging	Het
Ugt2b1	G	T	5: 87,073,821 (GRCm39)	Y179*	probably null	Het
Vmn2r27	T	A	6: 124,201,141 (GRCm39)	D272V	probably benign	Het
Vmn2r3	T	C	3: 64,178,774 (GRCm39)	E497G	probably benign	Het
Zfp276	A	G	8: 123,991,716 (GRCm39)		probably benign	Het

Other mutations in Vmn2r91

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00983:Vmn2r91	APN	17	18,325,820 (GRCm39)	missense	probably benign	0.00
IGL01121:Vmn2r91	APN	17	18,356,766 (GRCm39)	missense	possibly damaging	0.94
IGL01464:Vmn2r91	APN	17	18,327,864 (GRCm39)	missense	probably null	0.00
IGL02003:Vmn2r91	APN	17	18,327,921 (GRCm39)	missense	probably benign
IGL02709:Vmn2r91	APN	17	18,325,711 (GRCm39)	missense	possibly damaging	0.74
IGL02795:Vmn2r91	APN	17	18,305,539 (GRCm39)	missense	probably benign	0.01
IGL02813:Vmn2r91	APN	17	18,356,348 (GRCm39)	missense	possibly damaging	0.91
IGL02830:Vmn2r91	APN	17	18,356,884 (GRCm39)	missense	probably benign	0.01
IGL03130:Vmn2r91	APN	17	18,330,373 (GRCm39)	splice site	probably benign
BB006:Vmn2r91	UTSW	17	18,327,906 (GRCm39)	missense	probably damaging	1.00
BB016:Vmn2r91	UTSW	17	18,327,906 (GRCm39)	missense	probably damaging	1.00
R0164:Vmn2r91	UTSW	17	18,326,399 (GRCm39)	missense	probably benign	0.00
R0164:Vmn2r91	UTSW	17	18,326,399 (GRCm39)	missense	probably benign	0.00
R0393:Vmn2r91	UTSW	17	18,325,712 (GRCm39)	missense	probably damaging	1.00
R1142:Vmn2r91	UTSW	17	18,356,705 (GRCm39)	missense	probably damaging	1.00
R1603:Vmn2r91	UTSW	17	18,326,405 (GRCm39)	missense	probably benign	0.04
R1992:Vmn2r91	UTSW	17	18,356,142 (GRCm39)	missense	probably damaging	1.00
R2182:Vmn2r91	UTSW	17	18,325,691 (GRCm39)	missense	possibly damaging	0.94
R2424:Vmn2r91	UTSW	17	18,356,431 (GRCm39)	nonsense	probably null
R2512:Vmn2r91	UTSW	17	18,356,048 (GRCm39)	missense	probably benign
R2885:Vmn2r91	UTSW	17	18,325,628 (GRCm39)	missense	probably benign	0.00
R2909:Vmn2r91	UTSW	17	18,356,661 (GRCm39)	missense	probably damaging	1.00
R3009:Vmn2r91	UTSW	17	18,325,717 (GRCm39)	missense	probably benign	0.11
R3079:Vmn2r91	UTSW	17	18,355,973 (GRCm39)	splice site	probably null
R3080:Vmn2r91	UTSW	17	18,355,973 (GRCm39)	splice site	probably null
R3434:Vmn2r91	UTSW	17	18,330,370 (GRCm39)	splice site	probably benign
R3723:Vmn2r91	UTSW	17	18,305,540 (GRCm39)	critical splice donor site	probably null
R3829:Vmn2r91	UTSW	17	18,325,759 (GRCm39)	missense	probably damaging	1.00
R3845:Vmn2r91	UTSW	17	18,327,860 (GRCm39)	missense	probably benign	0.00
R3846:Vmn2r91	UTSW	17	18,327,860 (GRCm39)	missense	probably benign	0.00
R4118:Vmn2r91	UTSW	17	18,330,358 (GRCm39)	missense	probably damaging	1.00
R4285:Vmn2r91	UTSW	17	18,356,030 (GRCm39)	missense	probably benign	0.00
R4729:Vmn2r91	UTSW	17	18,327,906 (GRCm39)	missense	probably damaging	1.00
R4793:Vmn2r91	UTSW	17	18,325,658 (GRCm39)	missense	probably damaging	1.00
R4932:Vmn2r91	UTSW	17	18,356,751 (GRCm39)	missense	possibly damaging	0.84
R5016:Vmn2r91	UTSW	17	18,330,322 (GRCm39)	nonsense	probably null
R5605:Vmn2r91	UTSW	17	18,356,763 (GRCm39)	missense	probably damaging	1.00
R5815:Vmn2r91	UTSW	17	18,326,464 (GRCm39)	missense	probably benign	0.01
R6146:Vmn2r91	UTSW	17	18,356,518 (GRCm39)	missense	probably benign	0.07
R6187:Vmn2r91	UTSW	17	18,326,888 (GRCm39)	missense	probably benign	0.05
R6426:Vmn2r91	UTSW	17	18,355,865 (GRCm39)	splice site	probably null
R6450:Vmn2r91	UTSW	17	18,305,527 (GRCm39)	missense	probably damaging	0.98
R6767:Vmn2r91	UTSW	17	18,327,807 (GRCm39)	missense	probably damaging	0.98
R6986:Vmn2r91	UTSW	17	18,356,271 (GRCm39)	missense	probably benign	0.10
R7112:Vmn2r91	UTSW	17	18,325,880 (GRCm39)	missense	possibly damaging	0.83
R7178:Vmn2r91	UTSW	17	18,356,424 (GRCm39)	missense	probably damaging	1.00
R7330:Vmn2r91	UTSW	17	18,326,429 (GRCm39)	missense	probably damaging	1.00
R7368:Vmn2r91	UTSW	17	18,356,540 (GRCm39)	missense	possibly damaging	0.75
R7380:Vmn2r91	UTSW	17	18,356,838 (GRCm39)	nonsense	probably null
R7397:Vmn2r91	UTSW	17	18,356,060 (GRCm39)	missense	probably benign	0.02
R7625:Vmn2r91	UTSW	17	18,325,693 (GRCm39)	missense	probably damaging	1.00
R7739:Vmn2r91	UTSW	17	18,356,080 (GRCm39)	missense	probably benign	0.00
R7749:Vmn2r91	UTSW	17	18,356,540 (GRCm39)	missense	possibly damaging	0.75
R7755:Vmn2r91	UTSW	17	18,330,311 (GRCm39)	missense	possibly damaging	0.88
R7929:Vmn2r91	UTSW	17	18,327,906 (GRCm39)	missense	probably damaging	1.00
R7981:Vmn2r91	UTSW	17	18,327,887 (GRCm39)	missense	probably benign	0.02
R8211:Vmn2r91	UTSW	17	18,326,762 (GRCm39)	missense	probably damaging	1.00
R8325:Vmn2r91	UTSW	17	18,356,625 (GRCm39)	missense	probably damaging	1.00
R8781:Vmn2r91	UTSW	17	18,305,323 (GRCm39)	missense	possibly damaging	0.78
R8974:Vmn2r91	UTSW	17	18,325,636 (GRCm39)	missense	probably benign	0.27
R9047:Vmn2r91	UTSW	17	18,326,296 (GRCm39)	missense	probably benign	0.00
R9048:Vmn2r91	UTSW	17	18,356,122 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r91	UTSW	17	18,327,905 (GRCm39)	missense	probably damaging	1.00
R9211:Vmn2r91	UTSW	17	18,356,819 (GRCm39)	nonsense	probably null
R9555:Vmn2r91	UTSW	17	18,325,792 (GRCm39)	missense	possibly damaging	0.78
R9616:Vmn2r91	UTSW	17	18,356,305 (GRCm39)	missense	possibly damaging	0.81

Predicted Primers

PCR Primer
(F):5'- GGACACATCATCATTTTGTGCAAC -3'
(R):5'- CATGGACAATAAATGGCTTTCAGAC -3'

Sequencing Primer
(F):5'- CATTTTGTGCAACAAGGGCTCAG -3'
(R):5'- ATTTTAGCAACTGGAATGTG -3'

Posted On

2016-06-06