Mutagenetix > Incidental Mutation

Incidental Mutation 'R5116:Zfp932'

394187

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

042704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R5116 (G1)

Quality Score

128

Status

Validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 110157242 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 280 (D280E)

Ref Sequence

ENSEMBL: ENSMUSP00000108155 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112536
AA Change: D280E

PolyPhen 2 Score 0.049 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: D280E

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112540
AA Change: D313E

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: D313E

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187241
AA Change: D313E

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: D313E

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.8%
20x: 90.5%

Validation Efficiency

97% (59/61)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb9	T	C	5: 124,216,930 (GRCm39)	H429R	probably damaging	Het
Adam39	G	T	8: 41,278,038 (GRCm39)	C143F	probably damaging	Het
Adamts19	T	C	18: 59,036,066 (GRCm39)	V417A	possibly damaging	Het
Adcy10	C	A	1: 165,347,069 (GRCm39)	A362E	probably damaging	Het
Akap6	A	G	12: 53,188,298 (GRCm39)	E1904G	probably benign	Het
Aldh18a1	A	G	19: 40,541,949 (GRCm39)	M89T	probably benign	Het
Alyref	G	T	11: 120,488,554 (GRCm39)	F91L	probably benign	Het
Capn3	G	T	2: 120,315,773 (GRCm39)	M248I	probably benign	Het
Catsper4	A	T	4: 133,953,991 (GRCm39)	I56N	probably damaging	Het
Cckbr	T	A	7: 105,082,862 (GRCm39)	I75N	probably damaging	Het
Ccr8	T	C	9: 119,923,095 (GRCm39)	I70T	probably benign	Het
Cdh24	T	C	14: 54,873,870 (GRCm39)	D428G	probably benign	Het
Cdk5rap2	G	T	4: 70,225,475 (GRCm39)	Q557K	possibly damaging	Het
Clip1	C	T	5: 123,768,770 (GRCm39)	A610T	probably benign	Het
Dnajc16	A	T	4: 141,495,280 (GRCm39)	Y479*	probably null	Het
Fpr-rs3	A	G	17: 20,844,562 (GRCm39)	V193A	probably benign	Het
Gm29125	T	C	1: 80,361,690 (GRCm39)		noncoding transcript	Het
H1f4	A	G	13: 23,806,270 (GRCm39)	Y71H	probably damaging	Het
Ifi208	A	G	1: 173,505,549 (GRCm39)		probably benign	Het
Immp1l	G	A	2: 105,795,640 (GRCm39)	R155H	probably benign	Het
Itgad	C	A	7: 127,803,065 (GRCm39)	T7K	probably damaging	Het
Itgb3	T	C	11: 104,531,903 (GRCm39)	V370A	probably benign	Het
Jak1	A	G	4: 101,012,310 (GRCm39)	I1053T	probably benign	Het
Kif21b	A	G	1: 136,080,521 (GRCm39)	R572G	probably damaging	Het
Lama2	A	T	10: 26,994,556 (GRCm39)	D1784E	probably benign	Het
Ltbp2	A	T	12: 84,856,511 (GRCm39)	V651D	probably damaging	Het
Mrpl47	A	G	3: 32,787,750 (GRCm39)	L100S	probably damaging	Het
Mx1	T	G	16: 97,258,679 (GRCm39)	N6T	possibly damaging	Het
Nde1	T	A	16: 14,001,351 (GRCm39)	M133K	probably benign	Het
Nlrc5	T	C	8: 95,208,488 (GRCm39)	L778P	probably damaging	Het
Odad3	A	G	9: 21,901,424 (GRCm39)	*595Q	probably null	Het
Or52e19b	G	A	7: 103,033,071 (GRCm39)	T46I	probably benign	Het
Or5l13	G	A	2: 87,779,873 (GRCm39)	R235C	probably benign	Het
Or6c3b	A	G	10: 129,527,266 (GRCm39)	S215P	probably damaging	Het
Or7g32	A	G	9: 19,389,094 (GRCm39)	S151P	possibly damaging	Het
Or8b38	T	C	9: 37,972,634 (GRCm39)	V6A	probably benign	Het
Otog	T	C	7: 45,923,191 (GRCm39)	V1022A	probably benign	Het
Pcdhac1	G	A	18: 37,224,500 (GRCm39)	V438M	probably damaging	Het
Pcsk5	G	A	19: 17,440,798 (GRCm39)	S1264F	possibly damaging	Het
Pfas	T	C	11: 68,881,816 (GRCm39)		probably benign	Het
Pigr	T	A	1: 130,776,768 (GRCm39)	F648I	probably benign	Het
Pla2r1	A	C	2: 60,279,250 (GRCm39)	Y777D	probably damaging	Het
Pnpla7	G	T	2: 24,911,982 (GRCm39)	G716V	probably damaging	Het
Rab11fip5	C	T	6: 85,325,789 (GRCm39)	E206K	probably damaging	Het
S100a10	A	G	3: 93,468,247 (GRCm39)		probably null	Het
Slc35f1	T	C	10: 52,897,991 (GRCm39)	I134T	probably benign	Het
Smarcd1	C	T	15: 99,600,369 (GRCm39)	A56V	probably benign	Het
Snrk	A	G	9: 121,989,396 (GRCm39)	T247A	probably benign	Het
Srgap1	A	G	10: 121,628,284 (GRCm39)	L896P	possibly damaging	Het
Tmem19	A	T	10: 115,179,651 (GRCm39)	F167I	probably benign	Het
Tmprss11f	T	C	5: 86,687,555 (GRCm39)	S118G	probably benign	Het
Tnc	A	G	4: 63,885,452 (GRCm39)		probably null	Het
Trem3	T	C	17: 48,556,580 (GRCm39)	L17P	probably benign	Het
Upp2	A	G	2: 58,661,554 (GRCm39)	Y67C	probably damaging	Het
Zfp276	A	G	8: 123,991,716 (GRCm39)		probably benign	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4551:Zfp932	UTSW	5	110,157,505 (GRCm39)	missense	probably benign	0.03
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7437:Zfp932	UTSW	5	110,157,880 (GRCm39)	missense	probably benign	0.10
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9083:Zfp932	UTSW	5	110,157,100 (GRCm39)	missense	probably damaging	1.00
R9142:Zfp932	UTSW	5	110,157,520 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00
R9640:Zfp932	UTSW	5	110,157,064 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGCCTTTGTAGGTCAGAATGATCT -3'
(R):5'- CGTGCAAAGGCTTTACCACATTC -3'

Sequencing Primer
(F):5'- AGCCTTTGTATGTAATGCCAGTC -3'
(R):5'- GTAAGGCTTCTCTCCAGTATGAATTC -3'

Posted On

2016-06-15