Mutagenetix > Incidental Mutation

Incidental Mutation 'R5129:Zfp874a'

394920

Institutional Source

Beutler Lab

Gene Symbol

Zfp874a

Ensembl Gene

ENSMUSG00000069206

Gene Name

zinc finger protein 874a

Synonyms

C330011K17Rik, Zfp874, Rslcan15

MMRRC Submission

042717-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R5129 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67588550-67599743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 67591100 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 195 (C195S)

Ref Sequence

ENSEMBL: ENSMUSP00000074735 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057241] [ENSMUST00000075255] [ENSMUST00000180580] [ENSMUST00000223682] [ENSMUST00000225479]

AlphaFold

Q8BX23

Predicted Effect

probably benign
Transcript: ENSMUST00000057241

SMART Domains

Protein: ENSMUSP00000077307
Gene: ENSMUSG00000069206

Domain	Start	End	E-Value	Type
KRAB	5	65	1.73e-27	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000075255
AA Change: C195S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000074735
Gene: ENSMUSG00000069206
AA Change: C195S

Domain	Start	End	E-Value	Type
KRAB	5	65	1.73e-27	SMART
ZnF_C2H2	137	159	9.58e-3	SMART
ZnF_C2H2	165	187	5.99e-4	SMART
ZnF_C2H2	193	215	7.26e-3	SMART
ZnF_C2H2	221	243	2.4e-3	SMART
ZnF_C2H2	249	269	1.88e2	SMART
ZnF_C2H2	277	299	5.21e-4	SMART
ZnF_C2H2	305	327	2.12e-4	SMART
ZnF_C2H2	333	355	2.12e-4	SMART
ZnF_C2H2	361	383	8.47e-4	SMART
ZnF_C2H2	389	411	1.79e-2	SMART
ZnF_C2H2	417	438	2.86e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180580

SMART Domains

Protein: ENSMUSP00000137953
Gene: ENSMUSG00000069206

Domain	Start	End	E-Value	Type
KRAB	5	65	1.73e-27	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000223682

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224239

Predicted Effect

probably benign
Transcript: ENSMUST00000225479

Predicted Effect

probably benign
Transcript: ENSMUST00000225778

Meta Mutation Damage Score

0.3288

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.8%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	G	7: 29,260,786 (GRCm39)		noncoding transcript	Het
Abcf1	C	T	17: 36,271,687 (GRCm39)		probably benign	Het
Adamts14	T	A	10: 61,085,397 (GRCm39)	D209V	probably benign	Het
Aldh3b1	A	G	19: 3,965,336 (GRCm39)	F392L	probably benign	Het
Atp1a2	A	T	1: 172,103,522 (GRCm39)	D999E	probably benign	Het
Capn7	T	C	14: 31,066,468 (GRCm39)	V94A	probably damaging	Het
Cdh13	A	G	8: 119,821,954 (GRCm39)	D271G	probably damaging	Het
Cntnap5b	G	A	1: 100,306,815 (GRCm39)	G844D	probably damaging	Het
Col6a4	T	C	9: 105,890,576 (GRCm39)	E1906G	probably damaging	Het
Daw1	T	C	1: 83,183,624 (GRCm39)	Y225H	probably damaging	Het
Dvl3	T	A	16: 20,336,090 (GRCm39)	M49K	possibly damaging	Het
Eef1b2	T	G	1: 63,218,739 (GRCm39)	S175A	probably damaging	Het
Epb41l1	A	T	2: 156,351,201 (GRCm39)	Y425F	possibly damaging	Het
Fam43b	A	T	4: 138,122,783 (GRCm39)	L179*	probably null	Het
Fbxw28	A	G	9: 109,155,671 (GRCm39)	L314P	probably damaging	Het
Fstl4	C	A	11: 53,077,266 (GRCm39)	D674E	probably damaging	Het
G3bp1	T	C	11: 55,379,942 (GRCm39)	V92A	possibly damaging	Het
Gm3604	A	T	13: 62,517,588 (GRCm39)	Y257N	probably benign	Het
Gm5900	A	G	7: 104,599,223 (GRCm39)		noncoding transcript	Het
Hectd4	G	A	5: 121,481,573 (GRCm39)	V3041M	possibly damaging	Het
Hivep2	A	G	10: 14,006,608 (GRCm39)	K1069E	probably damaging	Het
Hlf	T	C	11: 90,281,078 (GRCm39)	D38G	probably benign	Het
Hsd3b7	C	A	7: 127,400,306 (GRCm39)	C18*	probably null	Het
Kdm5a	T	C	6: 120,381,983 (GRCm39)	C676R	probably damaging	Het
Klhdc7b	A	G	15: 89,272,751 (GRCm39)	Y1211C	probably damaging	Het
Krt78	C	A	15: 101,856,015 (GRCm39)	V599L	possibly damaging	Het
Mroh9	C	G	1: 162,888,329 (GRCm39)	G249R	probably damaging	Het
Nbas	T	C	12: 13,440,961 (GRCm39)	L1097P	probably damaging	Het
Nf2	A	C	11: 4,766,145 (GRCm39)	D87E	probably benign	Het
Or10d4c	T	A	9: 39,558,790 (GRCm39)	I256N	probably benign	Het
Or14j2	T	C	17: 37,886,071 (GRCm39)	Y81C	probably damaging	Het
Ppil3	A	G	1: 58,479,992 (GRCm39)		probably benign	Het
Prrc2a	T	C	17: 35,379,154 (GRCm39)	E276G	unknown	Het
Semp2l1	C	A	1: 32,584,560 (GRCm39)	R450M	probably damaging	Het
Semp2l1	T	A	1: 32,584,561 (GRCm39)	R450W	probably damaging	Het
Spata16	A	G	3: 26,721,713 (GRCm39)	E78G	probably damaging	Het
Tlr3	A	T	8: 45,856,018 (GRCm39)	I54K	probably damaging	Het
Tmem72	A	G	6: 116,678,974 (GRCm39)	L34P	probably damaging	Het
Triobp	A	G	15: 78,845,296 (GRCm39)	R213G	probably benign	Het
Tyrp1	T	A	4: 80,764,844 (GRCm39)	V7D	probably damaging	Het
Uhrf2	A	G	19: 30,052,621 (GRCm39)	I372V	probably benign	Het
Uty	T	C	Y: 1,158,592 (GRCm39)	T484A	probably benign	Het
Vcan	T	C	13: 89,838,359 (GRCm39)	D2395G	probably damaging	Het
Vmn1r225	T	A	17: 20,723,378 (GRCm39)	I273N	probably damaging	Het
Zfp451	C	T	1: 33,842,014 (GRCm39)		probably benign	Het
Zfp457	T	C	13: 67,441,420 (GRCm39)	E385G	probably benign	Het
Zfp74	C	T	7: 29,631,880 (GRCm39)	M121I	probably benign	Het
Zfp866	A	T	8: 70,220,359 (GRCm39)		probably null	Het

Other mutations in Zfp874a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02490:Zfp874a	APN	13	67,590,819 (GRCm39)	nonsense	probably null
R1052:Zfp874a	UTSW	13	67,590,539 (GRCm39)	missense	possibly damaging	0.89
R1991:Zfp874a	UTSW	13	67,590,623 (GRCm39)	missense	probably benign	0.03
R2085:Zfp874a	UTSW	13	67,590,967 (GRCm39)	missense	probably damaging	1.00
R2103:Zfp874a	UTSW	13	67,590,623 (GRCm39)	missense	probably benign	0.03
R5336:Zfp874a	UTSW	13	67,590,799 (GRCm39)	missense	probably damaging	1.00
R5423:Zfp874a	UTSW	13	67,590,473 (GRCm39)	missense	possibly damaging	0.75
R5713:Zfp874a	UTSW	13	67,597,476 (GRCm39)	missense	probably benign	0.16
R5855:Zfp874a	UTSW	13	67,590,812 (GRCm39)	missense	probably benign	0.03
R6496:Zfp874a	UTSW	13	67,590,694 (GRCm39)	missense	possibly damaging	0.62
R7046:Zfp874a	UTSW	13	67,590,418 (GRCm39)	missense	probably damaging	1.00
R7065:Zfp874a	UTSW	13	67,590,401 (GRCm39)	missense	probably damaging	0.97
R7233:Zfp874a	UTSW	13	67,590,776 (GRCm39)	missense	possibly damaging	0.83
R7271:Zfp874a	UTSW	13	67,591,415 (GRCm39)	missense	probably benign	0.00
R7465:Zfp874a	UTSW	13	67,590,376 (GRCm39)	missense	probably damaging	0.99
R7468:Zfp874a	UTSW	13	67,573,723 (GRCm39)	splice site	probably null
R8108:Zfp874a	UTSW	13	67,591,353 (GRCm39)	nonsense	probably null
R8109:Zfp874a	UTSW	13	67,597,563 (GRCm39)	missense	possibly damaging	0.46
R8843:Zfp874a	UTSW	13	67,590,764 (GRCm39)	missense	probably damaging	1.00
R8859:Zfp874a	UTSW	13	67,590,647 (GRCm39)	missense	probably benign	0.17
R9137:Zfp874a	UTSW	13	67,590,841 (GRCm39)	missense	probably damaging	1.00
R9673:Zfp874a	UTSW	13	67,591,156 (GRCm39)	missense	probably benign	0.03
X0063:Zfp874a	UTSW	13	67,591,106 (GRCm39)	missense	probably damaging	1.00
Z1177:Zfp874a	UTSW	13	67,590,781 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCCTAAGATATGAACGATAGCAAAAG -3'
(R):5'- GATCACAGCTCTCTAAGTATGCAATG -3'

Sequencing Primer
(F):5'- AGGATTTGCCACATTCTTGACAC -3'
(R):5'- GCTCTCTAAGTATGCAATGCCAGAG -3'

Posted On

2016-06-21