Mutagenetix > Incidental Mutation

Incidental Mutation 'R5145:Adgrg7'

395096

Institutional Source

Beutler Lab

Gene Symbol

Adgrg7

Ensembl Gene

ENSMUSG00000022755

Gene Name

adhesion G protein-coupled receptor G7

Synonyms

9130020O16Rik, Gpr128

MMRRC Submission

042729-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5145 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

56544972-56616218 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 56562682 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 552 (I552L)

Ref Sequence

ENSEMBL: ENSMUSP00000023437 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023437]

AlphaFold

Q8BM96

Predicted Effect

probably benign
Transcript: ENSMUST00000023437
AA Change: I552L

PolyPhen 2 Score 0.039 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000023437
Gene: ENSMUSG00000022755
AA Change: I552L

Domain	Start	End	E-Value	Type
transmembrane domain	12	34	N/A	INTRINSIC
SCOP:d1edmb_	52	76	1e-3	SMART
GPS	376	424	6.16e-8	SMART
Pfam:7tm_2	428	712	4.5e-40	PFAM

Meta Mutation Damage Score

0.1860

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 94.8%

Validation Efficiency

100% (37/37)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced body weight gain and increased frequency of peristalsis and slow wave potential in the small intestine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts12	G	A	15: 11,285,962 (GRCm39)	G724R	probably damaging	Het
Ankrd11	A	G	8: 123,617,943 (GRCm39)		probably benign	Het
Col12a1	T	C	9: 79,613,582 (GRCm39)	T88A	probably benign	Het
Col6a5	T	G	9: 105,811,444 (GRCm39)	I692L	unknown	Het
Cry2	T	C	2: 92,243,405 (GRCm39)	I479V	probably benign	Het
Ddx21	T	C	10: 62,423,318 (GRCm39)		probably null	Het
Efcab12	T	A	6: 115,800,238 (GRCm39)	I262F	probably damaging	Het
Eif2ak2	T	C	17: 79,183,633 (GRCm39)	D72G	possibly damaging	Het
Fry	T	C	5: 150,293,689 (GRCm39)	F461L	probably damaging	Het
Gm38706	G	A	6: 130,460,731 (GRCm39)		noncoding transcript	Het
Gm9772	C	T	17: 22,226,107 (GRCm39)	C59Y	probably damaging	Het
Meig1	T	C	2: 3,410,263 (GRCm39)	E79G	probably damaging	Het
Mfng	C	T	15: 78,648,588 (GRCm39)	R163H	probably benign	Het
Mfsd2b	T	A	12: 4,915,908 (GRCm39)		probably benign	Het
Nfatc2	A	C	2: 168,431,987 (GRCm39)	I42S	probably benign	Het
Nlgn3	G	A	X: 100,361,891 (GRCm39)	V287I	probably benign	Het
Npy6r	C	T	18: 44,409,686 (GRCm39)	T369I	probably benign	Het
Or7a36	A	G	10: 78,820,143 (GRCm39)	E173G	probably benign	Het
Ptprb	A	G	10: 116,179,820 (GRCm39)	T1413A	probably benign	Het
Ptprc	A	G	1: 138,017,304 (GRCm39)	S624P	probably benign	Het
Pum3	A	G	19: 27,377,169 (GRCm39)	V441A	probably damaging	Het
Ranbp2	T	A	10: 58,315,860 (GRCm39)	D2193E	probably damaging	Het
Rbl1	A	T	2: 157,017,397 (GRCm39)		probably benign	Het
Rnf167	T	C	11: 70,540,906 (GRCm39)		probably benign	Het
Sdr9c7	T	C	10: 127,738,259 (GRCm39)	V179A	probably damaging	Het
Sema3c	A	G	5: 17,932,615 (GRCm39)	N706S	possibly damaging	Het
Stt3a	T	C	9: 36,646,762 (GRCm39)	Y617C	probably damaging	Het
Tdrd6	T	A	17: 43,936,966 (GRCm39)	S1361C	probably damaging	Het
Tha1	A	C	11: 117,760,502 (GRCm39)	S241A	probably damaging	Het
Tti1	G	A	2: 157,850,432 (GRCm39)	A269V	probably benign	Het
Ugt2a1	A	G	5: 87,633,886 (GRCm39)		probably null	Het
Vmn2r10	T	C	5: 109,143,761 (GRCm39)	T730A	possibly damaging	Het

Other mutations in Adgrg7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01783:Adgrg7	APN	16	56,568,282 (GRCm39)	critical splice donor site	probably null
IGL03122:Adgrg7	APN	16	56,590,725 (GRCm39)	splice site	probably benign
orchard	UTSW	16	56,545,342 (GRCm39)	missense	probably damaging	1.00
sevin	UTSW	16	56,562,769 (GRCm39)	missense	probably damaging	1.00
R0632:Adgrg7	UTSW	16	56,562,952 (GRCm39)	missense	possibly damaging	0.89
R0673:Adgrg7	UTSW	16	56,593,849 (GRCm39)	missense	possibly damaging	0.48
R1690:Adgrg7	UTSW	16	56,615,993 (GRCm39)	missense	probably damaging	0.99
R2009:Adgrg7	UTSW	16	56,582,236 (GRCm39)	missense	probably benign	0.08
R2017:Adgrg7	UTSW	16	56,553,169 (GRCm39)	missense	probably benign	0.02
R2132:Adgrg7	UTSW	16	56,588,281 (GRCm39)	missense	probably damaging	1.00
R2153:Adgrg7	UTSW	16	56,572,791 (GRCm39)	missense	possibly damaging	0.75
R2229:Adgrg7	UTSW	16	56,572,766 (GRCm39)	missense	probably benign
R2436:Adgrg7	UTSW	16	56,582,308 (GRCm39)	missense	possibly damaging	0.78
R2878:Adgrg7	UTSW	16	56,570,817 (GRCm39)	missense	probably benign	0.14
R2981:Adgrg7	UTSW	16	56,570,769 (GRCm39)	critical splice donor site	probably null
R4014:Adgrg7	UTSW	16	56,562,651 (GRCm39)	missense	probably damaging	1.00
R4023:Adgrg7	UTSW	16	56,550,661 (GRCm39)	missense	probably damaging	1.00
R4024:Adgrg7	UTSW	16	56,550,661 (GRCm39)	missense	probably damaging	1.00
R4026:Adgrg7	UTSW	16	56,550,661 (GRCm39)	missense	probably damaging	1.00
R4551:Adgrg7	UTSW	16	56,568,375 (GRCm39)	missense	probably damaging	1.00
R4834:Adgrg7	UTSW	16	56,553,232 (GRCm39)	missense	probably damaging	1.00
R5041:Adgrg7	UTSW	16	56,550,711 (GRCm39)	missense	probably benign	0.21
R5377:Adgrg7	UTSW	16	56,550,669 (GRCm39)	missense	possibly damaging	0.68
R5549:Adgrg7	UTSW	16	56,570,790 (GRCm39)	missense	probably damaging	1.00
R5915:Adgrg7	UTSW	16	56,550,748 (GRCm39)	splice site	probably null
R5957:Adgrg7	UTSW	16	56,593,790 (GRCm39)	missense	probably damaging	0.96
R6146:Adgrg7	UTSW	16	56,593,829 (GRCm39)	missense	probably benign	0.21
R6198:Adgrg7	UTSW	16	56,597,556 (GRCm39)	missense	possibly damaging	0.64
R6233:Adgrg7	UTSW	16	56,599,005 (GRCm39)	missense	possibly damaging	0.52
R6337:Adgrg7	UTSW	16	56,572,788 (GRCm39)	missense	probably damaging	0.96
R6633:Adgrg7	UTSW	16	56,550,649 (GRCm39)	missense	probably benign	0.05
R6693:Adgrg7	UTSW	16	56,590,587 (GRCm39)	missense	probably damaging	0.97
R6812:Adgrg7	UTSW	16	56,616,161 (GRCm39)	start gained	probably benign
R6841:Adgrg7	UTSW	16	56,570,787 (GRCm39)	missense	probably damaging	1.00
R6868:Adgrg7	UTSW	16	56,593,839 (GRCm39)	missense	probably benign
R7076:Adgrg7	UTSW	16	56,562,769 (GRCm39)	missense	probably damaging	1.00
R7146:Adgrg7	UTSW	16	56,550,605 (GRCm39)	missense	probably damaging	1.00
R7232:Adgrg7	UTSW	16	56,597,515 (GRCm39)	splice site	probably null
R7266:Adgrg7	UTSW	16	56,590,674 (GRCm39)	missense	probably benign	0.00
R7376:Adgrg7	UTSW	16	56,545,342 (GRCm39)	missense	probably damaging	1.00
R7390:Adgrg7	UTSW	16	56,553,207 (GRCm39)	missense	probably damaging	0.98
R7401:Adgrg7	UTSW	16	56,562,781 (GRCm39)	missense	probably benign	0.43
R7496:Adgrg7	UTSW	16	56,553,220 (GRCm39)	missense	probably benign
R7540:Adgrg7	UTSW	16	56,570,792 (GRCm39)	missense	probably damaging	1.00
R8147:Adgrg7	UTSW	16	56,562,876 (GRCm39)	missense	probably damaging	1.00
R8354:Adgrg7	UTSW	16	56,616,045 (GRCm39)	start gained	probably benign
R8372:Adgrg7	UTSW	16	56,616,114 (GRCm39)	start gained	probably benign
R8393:Adgrg7	UTSW	16	56,582,477 (GRCm39)	missense	probably damaging	1.00
R8454:Adgrg7	UTSW	16	56,616,045 (GRCm39)	start gained	probably benign
R8723:Adgrg7	UTSW	16	56,582,282 (GRCm39)	missense	probably benign	0.00
R8891:Adgrg7	UTSW	16	56,572,762 (GRCm39)	missense	probably benign	0.03
R9017:Adgrg7	UTSW	16	56,553,211 (GRCm39)	missense	probably benign	0.01
R9570:Adgrg7	UTSW	16	56,570,813 (GRCm39)	missense	probably damaging	1.00
R9604:Adgrg7	UTSW	16	56,597,570 (GRCm39)	missense	probably damaging	0.99
R9628:Adgrg7	UTSW	16	56,553,193 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTTGATCCCTCACTGGAGAGTC -3'
(R):5'- AACCTCCTCTTTGTGTTTGGAATTG -3'

Sequencing Primer
(F):5'- CTCACTGGAGAGTCAGCTACTAG -3'
(R):5'- CCTCTTTGTGTTTGGAATTGAAAAC -3'

Posted On

2016-06-21