Mutagenetix > Incidental Mutation

Incidental Mutation 'R5150:Brd10'

395326

Institutional Source

Beutler Lab

Gene Symbol

Brd10

Ensembl Gene

ENSMUSG00000046138

Gene Name

bromodomain containing 10

Synonyms

9930021J03Rik, Gm9832

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

R5150 (G1)

Quality Score

119

Status

Validated

Chromosome

Chromosomal Location

29691802-29783389 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 29782950 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Serine at position 109 (A109S)

Ref Sequence

ENSEMBL: ENSMUSP00000135473 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099525] [ENSMUST00000177155]

AlphaFold

H3BKP8

Predicted Effect

probably benign
Transcript: ENSMUST00000099525

SMART Domains

Protein: ENSMUSP00000100503
Gene: ENSMUSG00000074909

Domain	Start	End	E-Value	Type
low complexity region	70	78	N/A	INTRINSIC
Pfam:HEAT_2	367	475	2.4e-12	PFAM
Pfam:HEAT_EZ	380	434	1.1e-9	PFAM
Pfam:HEAT	409	438	8.3e-7	PFAM
Pfam:HEAT	916	944	1.1e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000175726

Predicted Effect

probably damaging
Transcript: ENSMUST00000177155
AA Change: A109S

PolyPhen 2 Score 0.964 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000135473
Gene: ENSMUSG00000046138
AA Change: A109S

Domain	Start	End	E-Value	Type
low complexity region	4	66	N/A	INTRINSIC
BROMO	75	198	1.22e-3	SMART
low complexity region	205	219	N/A	INTRINSIC
low complexity region	280	298	N/A	INTRINSIC
low complexity region	428	441	N/A	INTRINSIC
coiled coil region	606	638	N/A	INTRINSIC
low complexity region	655	689	N/A	INTRINSIC
coiled coil region	697	729	N/A	INTRINSIC
low complexity region	1079	1093	N/A	INTRINSIC
internal_repeat_1	1097	1226	1.32e-7	PROSPERO
low complexity region	1228	1237	N/A	INTRINSIC
low complexity region	1319	1348	N/A	INTRINSIC
low complexity region	1401	1416	N/A	INTRINSIC
low complexity region	1535	1557	N/A	INTRINSIC
low complexity region	1580	1596	N/A	INTRINSIC
low complexity region	1665	1678	N/A	INTRINSIC
internal_repeat_1	1699	1843	1.32e-7	PROSPERO
low complexity region	1920	1926	N/A	INTRINSIC
low complexity region	1946	1960	N/A	INTRINSIC
low complexity region	1986	2004	N/A	INTRINSIC

Meta Mutation Damage Score

0.0675

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.9%

Validation Efficiency

93% (57/61)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam33	T	C	2: 130,895,117 (GRCm39)		probably benign	Het
Ahnak	T	C	19: 8,988,268 (GRCm39)	V3184A	possibly damaging	Het
Aoc1	A	G	6: 48,883,084 (GRCm39)	N320S	possibly damaging	Het
Bin2	T	C	15: 100,543,244 (GRCm39)	E313G	probably damaging	Het
C9orf72	A	G	4: 35,193,270 (GRCm39)	S228P	possibly damaging	Het
Ccdc47	T	C	11: 106,096,265 (GRCm39)	D253G	possibly damaging	Het
Ccdc73	A	G	2: 104,822,384 (GRCm39)	T778A	probably benign	Het
Cops3	C	A	11: 59,710,839 (GRCm39)	D377Y	probably damaging	Het
Cyp4a14	A	C	4: 115,350,806 (GRCm39)	V156G	probably damaging	Het
D6Ertd527e	C	G	6: 87,088,506 (GRCm39)	T223S	unknown	Het
Disp1	C	A	1: 182,871,063 (GRCm39)	M452I	probably damaging	Het
Fam210b	T	C	2: 172,193,468 (GRCm39)	Y94H	probably damaging	Het
Fbxo45	C	A	16: 32,065,524 (GRCm39)		probably benign	Het
Flrt2	T	A	12: 95,745,977 (GRCm39)	M105K	possibly damaging	Het
Gm26558	G	A	2: 70,491,656 (GRCm39)		probably benign	Het
Gpr83	T	G	9: 14,772,101 (GRCm39)	L91R	probably damaging	Het
Greb1l	TTTAATAACTT	TTT	18: 10,555,950 (GRCm39)		probably null	Het
Hmces	T	A	6: 87,910,217 (GRCm39)		probably null	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Ksr2	A	C	5: 117,693,074 (GRCm39)	E174A	probably damaging	Het
Lats1	A	G	10: 7,588,415 (GRCm39)	T1011A	probably benign	Het
Lrrc46	T	C	11: 96,926,957 (GRCm39)	D120G	probably damaging	Het
Ncstn	C	A	1: 171,895,151 (GRCm39)		probably benign	Het
Neb	T	C	2: 52,059,130 (GRCm39)	T6118A	probably benign	Het
Nipsnap2	A	C	5: 129,834,175 (GRCm39)	M272L	probably benign	Het
Nlgn2	C	T	11: 69,716,216 (GRCm39)	R775H	probably damaging	Het
Or13c7c	A	G	4: 43,836,301 (GRCm39)	L63P	probably damaging	Het
Or2n1c	A	C	17: 38,519,208 (GRCm39)	E24A	possibly damaging	Het
Or4f15	T	A	2: 111,814,366 (GRCm39)	T18S	probably benign	Het
Or4f61	T	A	2: 111,922,880 (GRCm39)	L55F	possibly damaging	Het
Or4g17	T	A	2: 111,209,598 (GRCm39)	D84E	probably damaging	Het
Or5b118	A	T	19: 13,448,794 (GRCm39)	Q153H	probably benign	Het
Pold1	A	G	7: 44,185,256 (GRCm39)	V750A	possibly damaging	Het
Prdm11	C	T	2: 92,805,817 (GRCm39)	E378K	probably damaging	Het
Ptpn9	A	G	9: 56,943,954 (GRCm39)	D276G	probably benign	Het
Rlf	A	T	4: 121,005,369 (GRCm39)	F1204I	probably damaging	Het
Robo1	C	A	16: 72,769,192 (GRCm39)	T537K	possibly damaging	Het
Sec31b	T	A	19: 44,508,970 (GRCm39)	M670L	probably benign	Het
Sephs1	T	A	2: 4,904,321 (GRCm39)	V233E	possibly damaging	Het
Serpinb2	T	C	1: 107,450,939 (GRCm39)		probably null	Het
Sf3b3	T	C	8: 111,550,008 (GRCm39)	Q670R	possibly damaging	Het
Slc8a2	A	C	7: 15,879,101 (GRCm39)	D529A	possibly damaging	Het
Sva	A	G	6: 42,019,093 (GRCm39)	N88D	probably benign	Het
Tcf15	G	T	2: 151,986,051 (GRCm39)	R169L	probably damaging	Het
Tfr2	A	G	5: 137,572,752 (GRCm39)	T188A	probably benign	Het
Tshz1	T	A	18: 84,031,340 (GRCm39)	K1023*	probably null	Het
Ttc28	A	G	5: 111,373,555 (GRCm39)	N966S	probably damaging	Het
Unc5c	T	A	3: 141,463,554 (GRCm39)	I225N	probably damaging	Het
Ush2a	T	C	1: 188,184,067 (GRCm39)	L1457S	possibly damaging	Het
Zbtb34	G	T	2: 33,301,133 (GRCm39)	H469Q	probably damaging	Het
Zfp692	T	C	11: 58,198,413 (GRCm39)	M1T	probably null	Het

Other mutations in Brd10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01311:Brd10	APN	19	29,731,420 (GRCm39)	missense	probably benign	0.33
IGL01535:Brd10	APN	19	29,731,212 (GRCm39)	missense	possibly damaging	0.53
IGL02019:Brd10	APN	19	29,694,463 (GRCm39)	missense	probably benign	0.28
IGL02034:Brd10	APN	19	29,694,259 (GRCm39)	missense	possibly damaging	0.73
IGL03114:Brd10	APN	19	29,694,532 (GRCm39)	missense	probably benign	0.18
IGL03382:Brd10	APN	19	29,694,676 (GRCm39)	missense	probably damaging	1.00
R0020:Brd10	UTSW	19	29,693,597 (GRCm39)	missense	probably damaging	0.98
R0020:Brd10	UTSW	19	29,693,597 (GRCm39)	missense	probably damaging	0.98
R0142:Brd10	UTSW	19	29,695,654 (GRCm39)	missense	possibly damaging	0.93
R0178:Brd10	UTSW	19	29,732,188 (GRCm39)	missense	probably damaging	1.00
R0453:Brd10	UTSW	19	29,731,068 (GRCm39)	missense	probably damaging	1.00
R0730:Brd10	UTSW	19	29,695,381 (GRCm39)	missense	probably benign	0.00
R0735:Brd10	UTSW	19	29,695,038 (GRCm39)	missense	possibly damaging	0.92
R0891:Brd10	UTSW	19	29,695,053 (GRCm39)	missense	probably damaging	1.00
R0894:Brd10	UTSW	19	29,697,974 (GRCm39)	splice site	probably benign
R1289:Brd10	UTSW	19	29,700,852 (GRCm39)	missense	probably benign	0.07
R1368:Brd10	UTSW	19	29,693,796 (GRCm39)	missense	probably damaging	0.97
R1387:Brd10	UTSW	19	29,700,853 (GRCm39)	missense	probably benign	0.15
R1483:Brd10	UTSW	19	29,696,745 (GRCm39)	missense	possibly damaging	0.93
R1526:Brd10	UTSW	19	29,712,545 (GRCm39)	missense	probably damaging	1.00
R1612:Brd10	UTSW	19	29,695,245 (GRCm39)	missense	possibly damaging	0.86
R1721:Brd10	UTSW	19	29,720,998 (GRCm39)	missense	probably damaging	0.99
R1764:Brd10	UTSW	19	29,696,560 (GRCm39)	missense	possibly damaging	0.53
R1822:Brd10	UTSW	19	29,693,814 (GRCm39)	missense	probably damaging	0.99
R1824:Brd10	UTSW	19	29,693,814 (GRCm39)	missense	probably damaging	0.99
R1859:Brd10	UTSW	19	29,732,323 (GRCm39)	missense	possibly damaging	0.53
R1868:Brd10	UTSW	19	29,720,998 (GRCm39)	missense	probably damaging	0.99
R1880:Brd10	UTSW	19	29,695,523 (GRCm39)	missense	probably benign	0.06
R1898:Brd10	UTSW	19	29,712,532 (GRCm39)	missense	possibly damaging	0.74
R1936:Brd10	UTSW	19	29,731,077 (GRCm39)	missense	possibly damaging	0.87
R1939:Brd10	UTSW	19	29,731,077 (GRCm39)	missense	possibly damaging	0.87
R1969:Brd10	UTSW	19	29,694,075 (GRCm39)	missense	possibly damaging	0.95
R2153:Brd10	UTSW	19	29,694,229 (GRCm39)	missense	probably benign	0.01
R2366:Brd10	UTSW	19	29,731,035 (GRCm39)	missense	probably damaging	0.99
R2379:Brd10	UTSW	19	29,696,275 (GRCm39)	missense	probably benign	0.01
R3107:Brd10	UTSW	19	29,700,847 (GRCm39)	missense	probably damaging	1.00
R4012:Brd10	UTSW	19	29,720,990 (GRCm39)	missense	probably damaging	1.00
R4222:Brd10	UTSW	19	29,696,149 (GRCm39)	missense	probably benign	0.18
R4328:Brd10	UTSW	19	29,720,961 (GRCm39)	missense	probably benign	0.00
R4329:Brd10	UTSW	19	29,720,961 (GRCm39)	missense	probably benign	0.00
R4387:Brd10	UTSW	19	29,782,715 (GRCm39)	unclassified	probably benign
R4688:Brd10	UTSW	19	29,694,501 (GRCm39)	missense	probably benign	0.33
R4796:Brd10	UTSW	19	29,731,018 (GRCm39)	missense	probably benign	0.33
R4820:Brd10	UTSW	19	29,695,809 (GRCm39)	missense	possibly damaging	0.53
R4832:Brd10	UTSW	19	29,694,616 (GRCm39)	missense	possibly damaging	0.53
R5056:Brd10	UTSW	19	29,694,759 (GRCm39)	missense	probably benign
R5224:Brd10	UTSW	19	29,696,450 (GRCm39)	missense	possibly damaging	0.73
R5306:Brd10	UTSW	19	29,707,230 (GRCm39)	intron	probably benign
R5460:Brd10	UTSW	19	29,732,250 (GRCm39)	missense	probably damaging	0.98
R5477:Brd10	UTSW	19	29,731,518 (GRCm39)	missense	probably benign	0.33
R5531:Brd10	UTSW	19	29,731,072 (GRCm39)	missense	possibly damaging	0.73
R5559:Brd10	UTSW	19	29,694,363 (GRCm39)	missense	possibly damaging	0.91
R5647:Brd10	UTSW	19	29,731,210 (GRCm39)	missense	possibly damaging	0.73
R5886:Brd10	UTSW	19	29,696,677 (GRCm39)	missense	probably benign	0.03
R6029:Brd10	UTSW	19	29,732,367 (GRCm39)	unclassified	probably benign
R6240:Brd10	UTSW	19	29,694,640 (GRCm39)	missense	probably benign	0.18
R6331:Brd10	UTSW	19	29,695,147 (GRCm39)	missense	probably benign	0.33
R6456:Brd10	UTSW	19	29,693,914 (GRCm39)	missense	possibly damaging	0.93
R6584:Brd10	UTSW	19	29,696,128 (GRCm39)	missense	possibly damaging	0.53
R6661:Brd10	UTSW	19	29,700,864 (GRCm39)	missense	possibly damaging	0.53
R6991:Brd10	UTSW	19	29,696,508 (GRCm39)	missense	possibly damaging	0.86
R7059:Brd10	UTSW	19	29,696,945 (GRCm39)	missense	probably benign	0.33
R7128:Brd10	UTSW	19	29,693,881 (GRCm39)	missense	possibly damaging	0.53
R7211:Brd10	UTSW	19	29,763,712 (GRCm39)	missense
R7471:Brd10	UTSW	19	29,707,139 (GRCm39)	splice site	probably null
R7686:Brd10	UTSW	19	29,694,870 (GRCm39)	missense	probably benign	0.34
R8012:Brd10	UTSW	19	29,695,534 (GRCm39)	missense	possibly damaging	0.73
R8203:Brd10	UTSW	19	29,693,443 (GRCm39)	missense	probably benign	0.18
R8353:Brd10	UTSW	19	29,731,242 (GRCm39)	missense	possibly damaging	0.53
R8672:Brd10	UTSW	19	29,731,564 (GRCm39)	missense	probably benign
R8755:Brd10	UTSW	19	29,693,890 (GRCm39)	missense	probably benign	0.03
R8918:Brd10	UTSW	19	29,696,841 (GRCm39)	missense	possibly damaging	0.53
R8954:Brd10	UTSW	19	29,696,126 (GRCm39)	missense	possibly damaging	0.72
R9038:Brd10	UTSW	19	29,731,900 (GRCm39)	missense	possibly damaging	0.86
R9195:Brd10	UTSW	19	29,763,703 (GRCm39)	missense
R9204:Brd10	UTSW	19	29,696,938 (GRCm39)	missense	possibly damaging	0.73
R9518:Brd10	UTSW	19	29,731,541 (GRCm39)	missense	possibly damaging	0.53
R9743:Brd10	UTSW	19	29,694,261 (GRCm39)	missense	probably benign
R9747:Brd10	UTSW	19	29,731,911 (GRCm39)	missense	possibly damaging	0.73
RF011:Brd10	UTSW	19	29,721,009 (GRCm39)	missense	possibly damaging	0.53
X0027:Brd10	UTSW	19	29,712,599 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGGAGATCCAGTGGTCGACA -3'
(R):5'- GAGTGCCTCCTCGGTGGA -3'

Sequencing Primer
(F):5'- GTAGCAGGTCTCCAGCATC -3'
(R):5'- TGGACGAGGAGGAGGAAGC -3'

Posted On

2016-06-21