Incidental Mutation 'R5165:Or5b97'
ID 395681
Institutional Source Beutler Lab
Gene Symbol Or5b97
Ensembl Gene ENSMUSG00000060303
Gene Name olfactory receptor family 5 subfamily B member 97
Synonyms MOR202-3, Olfr1447, GA_x6K02T2RE5P-3231251-3230331
MMRRC Submission 042746-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.063) question?
Stock # R5165 (G1)
Quality Score 225
Status Validated
Chromosome 19
Chromosomal Location 12878213-12879142 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 12878564 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Lysine at position 193 (N193K)
Ref Sequence ENSEMBL: ENSMUSP00000149392 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071484] [ENSMUST00000208343] [ENSMUST00000216989]
AlphaFold Q8VFX3
Predicted Effect probably benign
Transcript: ENSMUST00000071484
AA Change: N193K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000071419
Gene: ENSMUSG00000060303
AA Change: N193K

DomainStartEndE-ValueType
Pfam:7tm_4 32 309 5.3e-50 PFAM
Pfam:7TM_GPCR_Srsx 36 306 2.1e-7 PFAM
Pfam:7tm_1 42 291 7.4e-20 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000208343
Predicted Effect probably benign
Transcript: ENSMUST00000216989
AA Change: N193K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000218013
Predicted Effect noncoding transcript
Transcript: ENSMUST00000220222
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.3%
Validation Efficiency 98% (58/59)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 53 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actg2 T A 6: 83,503,814 (GRCm39) I77F probably benign Het
Actl6a G A 3: 32,774,357 (GRCm39) V285I probably benign Het
Adam9 A T 8: 25,457,190 (GRCm39) I646N possibly damaging Het
Ahnak T C 19: 8,993,029 (GRCm39) I4771T possibly damaging Het
Alas1 T C 9: 106,118,454 (GRCm39) T223A probably damaging Het
Apc2 A G 10: 80,151,684 (GRCm39) E2246G probably damaging Het
Atp1a1 A G 3: 101,489,105 (GRCm39) I795T probably benign Het
Ccn3 A G 15: 54,612,585 (GRCm39) D198G probably damaging Het
Cdhr4 T C 9: 107,874,829 (GRCm39) L633P probably damaging Het
Cep350 A G 1: 155,804,114 (GRCm39) S990P probably damaging Het
Cplx2 A G 13: 54,526,789 (GRCm39) I66V possibly damaging Het
Cx3cl1 T C 8: 95,506,504 (GRCm39) S170P probably benign Het
Cyth1 T C 11: 118,059,908 (GRCm39) N353S possibly damaging Het
Dapp1 T C 3: 137,644,976 (GRCm39) probably null Het
Dmwd G A 7: 18,811,960 (GRCm39) probably benign Het
Dsg1c A G 18: 20,410,080 (GRCm39) H516R probably damaging Het
Efemp2 T C 19: 5,525,439 (GRCm39) C39R probably damaging Het
Fnbp4 C G 2: 90,608,001 (GRCm39) Q908E possibly damaging Het
Foxred2 A G 15: 77,840,212 (GRCm39) V26A probably damaging Het
Gkap1 A G 13: 58,411,010 (GRCm39) probably null Het
Gstcd A C 3: 132,790,440 (GRCm39) V109G probably damaging Het
Hoxc9 T A 15: 102,892,432 (GRCm39) M215K probably damaging Het
Igkv4-80 A C 6: 68,993,649 (GRCm39) S81A probably benign Het
Kcnj9 A T 1: 172,150,724 (GRCm39) D296E probably benign Het
Kctd18 T C 1: 57,998,395 (GRCm39) Y68C probably damaging Het
Kif21a A T 15: 90,840,579 (GRCm39) M1179K probably benign Het
Lrfn5 T C 12: 61,886,410 (GRCm39) I66T possibly damaging Het
Lrp12 A G 15: 39,735,857 (GRCm39) S692P probably benign Het
Lrrc10 A T 10: 116,881,965 (GRCm39) N213I probably benign Het
Nceh1 G A 3: 27,295,677 (GRCm39) V313I probably benign Het
Nkx6-3 T A 8: 23,643,759 (GRCm39) H53Q probably damaging Het
Ntmt2 A G 1: 163,550,092 (GRCm39) I53T probably benign Het
Or14j7 A G 17: 38,235,252 (GRCm39) D265G probably benign Het
Or2y16 A G 11: 49,335,203 (GRCm39) H175R probably damaging Het
Or4f53 T A 2: 111,087,568 (GRCm39) V36E possibly damaging Het
Oxct1 A T 15: 4,083,251 (GRCm39) T157S possibly damaging Het
Pcdhga3 A G 18: 37,808,723 (GRCm39) E392G possibly damaging Het
Polr1a T G 6: 71,944,909 (GRCm39) Y1322D probably damaging Het
Prkdc T A 16: 15,496,136 (GRCm39) S776T probably damaging Het
Ralgapb A G 2: 158,307,832 (GRCm39) I1047V possibly damaging Het
Sertad4 A T 1: 192,529,130 (GRCm39) S229T possibly damaging Het
Shank2 T A 7: 143,963,373 (GRCm39) V327D possibly damaging Het
Skint6 A T 4: 112,722,865 (GRCm39) V904E possibly damaging Het
Slfn8 A T 11: 82,907,953 (GRCm39) Y197N probably damaging Het
Smo T A 6: 29,736,077 (GRCm39) L23Q unknown Het
Snx29 T A 16: 11,238,639 (GRCm39) M23K probably damaging Het
Synrg T C 11: 83,881,761 (GRCm39) S366P probably benign Het
Tomm40 A G 7: 19,447,592 (GRCm39) probably null Het
Tpcn1 T C 5: 120,696,010 (GRCm39) E81G probably damaging Het
Trappc1 A G 11: 69,215,060 (GRCm39) Q26R probably benign Het
Ttn T A 2: 76,606,900 (GRCm39) probably null Het
Usp25 T A 16: 76,873,293 (GRCm39) D450E possibly damaging Het
Zmynd19 T A 2: 24,848,201 (GRCm39) Y132* probably null Het
Other mutations in Or5b97
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00944:Or5b97 APN 19 12,878,719 (GRCm39) missense probably benign 0.40
IGL01704:Or5b97 APN 19 12,879,103 (GRCm39) missense probably benign 0.01
IGL01767:Or5b97 APN 19 12,879,112 (GRCm39) missense probably benign 0.12
IGL01969:Or5b97 APN 19 12,878,416 (GRCm39) missense possibly damaging 0.69
IGL02435:Or5b97 APN 19 12,878,391 (GRCm39) missense probably damaging 1.00
IGL02666:Or5b97 APN 19 12,878,221 (GRCm39) missense probably benign
IGL03034:Or5b97 APN 19 12,879,121 (GRCm39) missense possibly damaging 0.94
IGL03221:Or5b97 APN 19 12,878,905 (GRCm39) missense probably damaging 1.00
R0315:Or5b97 UTSW 19 12,878,598 (GRCm39) missense possibly damaging 0.66
R0550:Or5b97 UTSW 19 12,879,164 (GRCm39) splice site probably null
R0729:Or5b97 UTSW 19 12,878,259 (GRCm39) missense probably damaging 0.97
R1381:Or5b97 UTSW 19 12,878,320 (GRCm39) missense probably benign 0.00
R1669:Or5b97 UTSW 19 12,878,652 (GRCm39) missense possibly damaging 0.79
R1775:Or5b97 UTSW 19 12,878,599 (GRCm39) missense probably benign 0.02
R1918:Or5b97 UTSW 19 12,878,215 (GRCm39) makesense probably null
R2377:Or5b97 UTSW 19 12,878,217 (GRCm39) missense possibly damaging 0.45
R2406:Or5b97 UTSW 19 12,878,991 (GRCm39) missense probably benign 0.11
R2471:Or5b97 UTSW 19 12,878,679 (GRCm39) missense probably benign 0.00
R2484:Or5b97 UTSW 19 12,879,005 (GRCm39) missense probably benign 0.06
R2656:Or5b97 UTSW 19 12,879,030 (GRCm39) missense probably benign 0.37
R3888:Or5b97 UTSW 19 12,878,497 (GRCm39) missense probably benign 0.00
R4250:Or5b97 UTSW 19 12,878,368 (GRCm39) missense probably benign 0.09
R4545:Or5b97 UTSW 19 12,878,632 (GRCm39) nonsense probably null
R4895:Or5b97 UTSW 19 12,878,251 (GRCm39) missense probably damaging 1.00
R4956:Or5b97 UTSW 19 12,878,963 (GRCm39) missense probably damaging 0.99
R4991:Or5b97 UTSW 19 12,878,815 (GRCm39) missense probably damaging 0.98
R5044:Or5b97 UTSW 19 12,878,365 (GRCm39) missense probably damaging 1.00
R6025:Or5b97 UTSW 19 12,879,034 (GRCm39) missense probably benign 0.10
R6135:Or5b97 UTSW 19 12,878,803 (GRCm39) missense probably damaging 0.97
R6459:Or5b97 UTSW 19 12,878,369 (GRCm39) missense possibly damaging 0.94
R6733:Or5b97 UTSW 19 12,878,605 (GRCm39) missense probably damaging 1.00
R6789:Or5b97 UTSW 19 12,878,653 (GRCm39) missense probably benign 0.21
R6923:Or5b97 UTSW 19 12,878,676 (GRCm39) missense probably benign 0.04
R7310:Or5b97 UTSW 19 12,878,637 (GRCm39) missense probably damaging 1.00
R8552:Or5b97 UTSW 19 12,879,096 (GRCm39) missense probably damaging 0.98
R8699:Or5b97 UTSW 19 12,878,828 (GRCm39) missense possibly damaging 0.59
R8735:Or5b97 UTSW 19 12,878,274 (GRCm39) missense possibly damaging 0.85
R8955:Or5b97 UTSW 19 12,878,578 (GRCm39) missense probably benign 0.01
R9626:Or5b97 UTSW 19 12,878,600 (GRCm39) missense possibly damaging 0.88
R9631:Or5b97 UTSW 19 12,878,502 (GRCm39) missense possibly damaging 0.69
R9694:Or5b97 UTSW 19 12,879,021 (GRCm39) missense probably damaging 0.97
Predicted Primers PCR Primer
(F):5'- TGGAGCTGGGCTGTAAATAC -3'
(R):5'- TGTAAGCCCCTACACTATGCC -3'

Sequencing Primer
(F):5'- CTGGGCTGTAAATACATGAAAATGAC -3'
(R):5'- TGCCACCATCATGACTAAAAGTATG -3'
Posted On 2016-06-21