|Institutional Source||Beutler Lab|
|Gene Name||anti-silencing function 1B histone chaperone|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R5156 (G1)|
|Chromosomal Location||83955507-83970197 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 83955911 bp|
|Amino Acid Change||Phenylalanine to Serine at position 28 (F28S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000005607 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000005607] [ENSMUST00000098595]|
|Predicted Effect||probably damaging
AA Change: F28S
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: F28S
|Predicted Effect||probably benign
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.566|
|Coding Region Coverage||
|Validation Efficiency||100% (52/52)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mutant mice are viable and fertile and exhibit no overt abnormal phenotype. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Asf1b||
(F):5'- TATTTGAAGGGAGCGCGCTG -3'
(R):5'- AATGTACACTGGACAGAGCC -3'
(F):5'- AGACGCAGGGAGGTTTCC -3'
(R):5'- ACTGGACAGAGCCGGCTC -3'