Mutagenetix > Incidental Mutation

Incidental Mutation 'R4779:Tmem245'

397264

Institutional Source

Beutler Lab

Gene Symbol

Tmem245

Ensembl Gene

ENSMUSG00000055296

Gene Name

transmembrane protein 245

Synonyms

D730040F13Rik, A630051L19Rik

MMRRC Submission

045240-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.298) question?

Stock #

R4779 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

56866923-56947437 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 56936468 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 230 (S230T)

Ref Sequence

ENSEMBL: ENSMUSP00000067421 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068792] [ENSMUST00000107609]

AlphaFold

B1AZA5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000068792
AA Change: S230T

PolyPhen 2 Score 0.650 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000067421
Gene: ENSMUSG00000055296
AA Change: S230T

Domain	Start	End	E-Value	Type
low complexity region	4	25	N/A	INTRINSIC
transmembrane domain	50	72	N/A	INTRINSIC
transmembrane domain	109	131	N/A	INTRINSIC
transmembrane domain	144	166	N/A	INTRINSIC
transmembrane domain	181	203	N/A	INTRINSIC
transmembrane domain	212	231	N/A	INTRINSIC
transmembrane domain	235	252	N/A	INTRINSIC
low complexity region	309	330	N/A	INTRINSIC
transmembrane domain	351	373	N/A	INTRINSIC
transmembrane domain	457	479	N/A	INTRINSIC
Pfam:UPF0118	589	838	1.7e-14	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107609
AA Change: S230T

PolyPhen 2 Score 0.504 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000103234
Gene: ENSMUSG00000055296
AA Change: S230T

Domain	Start	End	E-Value	Type
low complexity region	4	25	N/A	INTRINSIC
transmembrane domain	50	72	N/A	INTRINSIC
transmembrane domain	109	131	N/A	INTRINSIC
transmembrane domain	144	166	N/A	INTRINSIC
transmembrane domain	181	203	N/A	INTRINSIC
transmembrane domain	212	231	N/A	INTRINSIC
transmembrane domain	235	252	N/A	INTRINSIC
low complexity region	309	330	N/A	INTRINSIC
transmembrane domain	351	373	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
Pfam:UPF0118	585	842	1.1e-12	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125082

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155625

Meta Mutation Damage Score

0.0745

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

98% (95/97)

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930562C15Rik	T	A	16: 4,667,613 (GRCm39)	S335T	unknown	Het
4933421I07Rik	T	A	7: 42,097,455 (GRCm39)	H13L	possibly damaging	Het
6030468B19Rik	A	G	11: 117,696,834 (GRCm39)	T185A	probably benign	Het
Abcc1	T	A	16: 14,228,635 (GRCm39)	V294E	probably benign	Het
Abhd16b	A	G	2: 181,135,253 (GRCm39)	T52A	possibly damaging	Het
Actl7a	A	T	4: 56,743,632 (GRCm39)	N53I	probably benign	Het
Ankmy1	T	C	1: 92,814,445 (GRCm39)	E354G	probably benign	Het
Arap1	T	C	7: 101,053,574 (GRCm39)	F1301S	probably damaging	Het
Arfgef1	A	T	1: 10,223,958 (GRCm39)	W1447R	probably damaging	Het
Atoh7	ATGGCGCT	AT	10: 62,936,187 (GRCm39)		probably benign	Het
Ccl24	T	A	5: 135,601,811 (GRCm39)	T6S	possibly damaging	Het
Cep152	G	T	2: 125,410,812 (GRCm39)	P1292Q	possibly damaging	Het
Cfap46	T	C	7: 139,239,731 (GRCm39)		probably benign	Het
Chd6	A	T	2: 160,791,477 (GRCm39)	S2627T	probably damaging	Het
Chd8	A	T	14: 52,468,963 (GRCm39)	S552T	probably damaging	Het
Ciita	C	A	16: 10,329,230 (GRCm39)	L502M	probably damaging	Het
Clic1	T	C	17: 35,271,463 (GRCm39)	F31S	probably damaging	Het
Cntnap1	T	A	11: 101,068,898 (GRCm39)	I147N	possibly damaging	Het
Cplane1	T	C	15: 8,248,322 (GRCm39)	S1624P	probably benign	Het
Crispld1	T	A	1: 17,819,831 (GRCm39)	D276E	probably benign	Het
Cspg4	A	T	9: 56,793,092 (GRCm39)	N276Y	probably damaging	Het
Cspg4b	A	T	13: 113,504,870 (GRCm39)	N2000Y	possibly damaging	Het
Cwf19l2	A	G	9: 3,410,035 (GRCm39)	M55V	possibly damaging	Het
Cyp2c69	T	C	19: 39,866,038 (GRCm39)	N185S	probably benign	Het
Dop1b	T	C	16: 93,553,969 (GRCm39)	I301T	probably damaging	Het
Epg5	T	C	18: 78,034,580 (GRCm39)	V1443A	probably benign	Het
Ephb4	T	C	5: 137,363,964 (GRCm39)	V612A	probably benign	Het
Fcgbp	T	A	7: 27,794,362 (GRCm39)	C1189S	probably damaging	Het
Fgfr2	T	G	7: 129,786,923 (GRCm39)		probably benign	Het
Fmn2	A	G	1: 174,437,461 (GRCm39)	E1144G	probably damaging	Het
Fmo3	T	C	1: 162,796,407 (GRCm39)	Y55C	probably damaging	Het
Frmpd1	A	G	4: 45,229,865 (GRCm39)	T11A	probably damaging	Het
Ghdc	G	T	11: 100,660,929 (GRCm39)	Q79K	possibly damaging	Het
Gm11677	C	T	11: 111,615,537 (GRCm39)		noncoding transcript	Het
Gm5591	T	C	7: 38,221,680 (GRCm39)	T130A	probably damaging	Het
Heg1	T	G	16: 33,540,142 (GRCm39)	D367E	probably benign	Het
Igkv13-84	C	T	6: 68,916,894 (GRCm39)	P64S	probably damaging	Het
Il10rb	T	A	16: 91,211,545 (GRCm39)	S128T	possibly damaging	Het
Il15ra	T	A	2: 11,723,117 (GRCm39)	I47N	probably damaging	Het
Il33	C	A	19: 29,936,311 (GRCm39)	S207*	probably null	Het
Itgb7	A	G	15: 102,132,848 (GRCm39)	Y155H	possibly damaging	Het
Kdm5a	A	G	6: 120,346,060 (GRCm39)		probably benign	Het
Kif11	G	A	19: 37,406,397 (GRCm39)	V987I	probably benign	Het
Krtap19-2	G	A	16: 88,670,762 (GRCm39)		probably benign	Het
Krtap24-1	T	A	16: 88,408,417 (GRCm39)	R236S	probably damaging	Het
L3mbtl2	A	G	15: 81,566,813 (GRCm39)	I406V	probably benign	Het
Lrp2	A	T	2: 69,290,059 (GRCm39)	H3593Q	possibly damaging	Het
Mavs	G	T	2: 131,082,285 (GRCm39)	W56C	probably damaging	Het
Mn1	C	T	5: 111,567,526 (GRCm39)	P499S	probably damaging	Het
Ntn5	T	A	7: 45,340,895 (GRCm39)	C178S	probably damaging	Het
Nufip2	A	G	11: 77,577,154 (GRCm39)	H34R	unknown	Het
Or5p51	T	A	7: 107,444,755 (GRCm39)	M62L	possibly damaging	Het
Or6f2	T	A	7: 139,756,363 (GRCm39)	L110Q	probably damaging	Het
Osbpl10	C	T	9: 114,938,598 (GRCm39)	S86L	probably damaging	Het
Pitpna	T	A	11: 75,511,153 (GRCm39)	M242K	possibly damaging	Het
Pnpla6	T	C	8: 3,572,838 (GRCm39)	V345A	probably benign	Het
Polk	A	G	13: 96,632,999 (GRCm39)		probably null	Het
Polr3k	A	G	2: 181,506,340 (GRCm39)	Y30C	probably damaging	Het
Pramel6	A	G	2: 87,339,941 (GRCm39)	H235R	probably benign	Het
Ptpra	G	A	2: 130,379,537 (GRCm39)	V364I	probably damaging	Het
Recql	A	G	6: 142,309,426 (GRCm39)		probably benign	Het
Ring1	T	C	17: 34,241,263 (GRCm39)		probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Samsn1	C	A	16: 75,744,177 (GRCm39)		noncoding transcript	Het
Scara5	A	G	14: 65,968,198 (GRCm39)	H157R	probably benign	Het
Sdc3	C	T	4: 130,546,376 (GRCm39)	P245L	probably damaging	Het
Slc18b1	T	A	10: 23,696,767 (GRCm39)	M318K	possibly damaging	Het
Slc26a10	C	T	10: 127,009,224 (GRCm39)	A638T	possibly damaging	Het
Slc35f2	T	A	9: 53,717,013 (GRCm39)	W259R	possibly damaging	Het
Spata13	G	A	14: 60,991,356 (GRCm39)	W366*	probably null	Het
Sptlc3	A	G	2: 139,431,509 (GRCm39)	T344A	probably benign	Het
Tap1	T	C	17: 34,412,865 (GRCm39)	V560A	probably damaging	Het
Tbc1d1	T	C	5: 64,435,389 (GRCm39)		probably null	Het
Tlr11	A	G	14: 50,598,707 (GRCm39)	Y231C	possibly damaging	Het
Tnnt3	C	T	7: 142,068,020 (GRCm39)		probably benign	Het
Traf7	C	G	17: 24,729,412 (GRCm39)		probably benign	Het
Tshz2	A	G	2: 169,804,601 (GRCm39)		probably benign	Het
Tshz3	T	C	7: 36,468,397 (GRCm39)	S129P	probably damaging	Het
Ttc6	A	T	12: 57,776,237 (GRCm39)	N1727I	probably damaging	Het
Ttc7b	A	G	12: 100,369,621 (GRCm39)	S383P	probably damaging	Het
Tulp3	T	A	6: 128,300,083 (GRCm39)	I448F	probably damaging	Het
Ube3b	T	C	5: 114,542,778 (GRCm39)		probably null	Het
Vav1	G	A	17: 57,603,552 (GRCm39)	V84I	probably damaging	Het
Vav3	A	T	3: 109,416,110 (GRCm39)	K243I	possibly damaging	Het
Vmn1r25	T	A	6: 57,956,011 (GRCm39)	I93F	probably damaging	Het
Vmn2r73	C	T	7: 85,520,923 (GRCm39)	W348*	probably null	Het
Zdbf2	A	G	1: 63,342,397 (GRCm39)	R259G	possibly damaging	Het

Other mutations in Tmem245

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02472:Tmem245	APN	4	56,899,119 (GRCm39)	missense	probably damaging	1.00
IGL02668:Tmem245	APN	4	56,925,081 (GRCm39)	missense	possibly damaging	0.86
IGL03093:Tmem245	APN	4	56,886,019 (GRCm39)	missense	probably damaging	1.00
Integral	UTSW	4	56,899,170 (GRCm39)	missense	possibly damaging	0.79
leibniz	UTSW	4	56,916,770 (GRCm39)	missense	probably benign	0.02
R0090:Tmem245	UTSW	4	56,899,410 (GRCm39)	missense	probably benign
R0116:Tmem245	UTSW	4	56,926,213 (GRCm39)	missense	probably benign	0.00
R0648:Tmem245	UTSW	4	56,906,270 (GRCm39)	missense	probably benign	0.38
R0864:Tmem245	UTSW	4	56,890,837 (GRCm39)	missense	probably damaging	1.00
R1102:Tmem245	UTSW	4	56,903,200 (GRCm39)	intron	probably benign
R1548:Tmem245	UTSW	4	56,906,233 (GRCm39)	nonsense	probably null
R1778:Tmem245	UTSW	4	56,903,968 (GRCm39)	missense	probably damaging	1.00
R1840:Tmem245	UTSW	4	56,903,947 (GRCm39)	missense	probably benign	0.03
R1942:Tmem245	UTSW	4	56,923,511 (GRCm39)	unclassified	probably benign
R1969:Tmem245	UTSW	4	56,937,964 (GRCm39)	missense	probably benign	0.01
R2341:Tmem245	UTSW	4	56,937,957 (GRCm39)	missense	probably damaging	1.00
R2364:Tmem245	UTSW	4	56,899,391 (GRCm39)	missense	probably damaging	0.96
R3848:Tmem245	UTSW	4	56,926,298 (GRCm39)	unclassified	probably benign
R4591:Tmem245	UTSW	4	56,910,204 (GRCm39)	missense	probably damaging	0.99
R4772:Tmem245	UTSW	4	56,937,989 (GRCm39)	splice site	probably null
R4860:Tmem245	UTSW	4	56,899,164 (GRCm39)	missense	probably damaging	1.00
R4860:Tmem245	UTSW	4	56,899,164 (GRCm39)	missense	probably damaging	1.00
R5049:Tmem245	UTSW	4	56,925,057 (GRCm39)	missense	probably benign	0.12
R5061:Tmem245	UTSW	4	56,946,945 (GRCm39)	missense	possibly damaging	0.94
R5199:Tmem245	UTSW	4	56,925,149 (GRCm39)	missense	probably benign	0.12
R5377:Tmem245	UTSW	4	56,947,084 (GRCm39)	missense	probably damaging	0.99
R5547:Tmem245	UTSW	4	56,910,156 (GRCm39)	critical splice donor site	probably null
R5846:Tmem245	UTSW	4	56,903,241 (GRCm39)	missense	probably benign	0.00
R5851:Tmem245	UTSW	4	56,916,770 (GRCm39)	missense	probably benign	0.02
R5991:Tmem245	UTSW	4	56,916,733 (GRCm39)	missense	probably damaging	1.00
R6314:Tmem245	UTSW	4	56,888,592 (GRCm39)	missense	possibly damaging	0.88
R6992:Tmem245	UTSW	4	56,937,940 (GRCm39)	missense	probably benign	0.03
R7172:Tmem245	UTSW	4	56,903,946 (GRCm39)	missense	possibly damaging	0.65
R7632:Tmem245	UTSW	4	56,916,787 (GRCm39)	missense	probably benign	0.00
R7660:Tmem245	UTSW	4	56,899,170 (GRCm39)	missense	possibly damaging	0.79
R7672:Tmem245	UTSW	4	56,947,069 (GRCm39)	missense	probably benign
R7735:Tmem245	UTSW	4	56,925,155 (GRCm39)	missense	probably benign	0.22
R7900:Tmem245	UTSW	4	56,924,973 (GRCm39)	splice site	probably null
R8280:Tmem245	UTSW	4	56,890,884 (GRCm39)	missense	possibly damaging	0.89
R8306:Tmem245	UTSW	4	56,886,037 (GRCm39)	missense	probably damaging	0.96
R8446:Tmem245	UTSW	4	56,906,261 (GRCm39)	missense	probably benign	0.24
R8447:Tmem245	UTSW	4	56,906,261 (GRCm39)	missense	probably benign	0.24
R8491:Tmem245	UTSW	4	56,906,261 (GRCm39)	missense	probably benign	0.24
R8524:Tmem245	UTSW	4	56,906,261 (GRCm39)	missense	probably benign	0.24
R8750:Tmem245	UTSW	4	56,886,141 (GRCm39)	missense	probably damaging	1.00
R8756:Tmem245	UTSW	4	56,899,025 (GRCm39)	critical splice donor site	probably null
R8899:Tmem245	UTSW	4	56,903,916 (GRCm39)	critical splice donor site	probably null
R9035:Tmem245	UTSW	4	56,922,384 (GRCm39)	intron	probably benign
R9267:Tmem245	UTSW	4	56,947,236 (GRCm39)	missense	probably benign	0.03
R9292:Tmem245	UTSW	4	56,926,173 (GRCm39)	unclassified	probably benign
R9292:Tmem245	UTSW	4	56,937,979 (GRCm39)	missense	probably benign	0.07
R9667:Tmem245	UTSW	4	56,947,119 (GRCm39)	missense	probably damaging	0.98
Z1189:Tmem245	UTSW	4	56,937,901 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTCTGTTTAGAAACCACTTCGGG -3'
(R):5'- AAGGGTAGGTTGGCACATGC -3'

Sequencing Primer
(F):5'- GAACAAGCTAGTCCTTTATGGCC -3'
(R):5'- GCCTTCATCCTTTGAGAGCAAGG -3'

Posted On

2016-07-05