Mutagenetix > Incidental Mutation

Incidental Mutation 'R5166:Zfp850'

397299

Institutional Source

Beutler Lab

Gene Symbol

Zfp850

Ensembl Gene

ENSMUSG00000096916

Gene Name

zinc finger protein 850

Synonyms

C130069I09Rik, Gm4636

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R5166 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

27684279-27713540 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 27689781 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 142 (C142*)

Ref Sequence

ENSEMBL: ENSMUSP00000141063 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099111] [ENSMUST00000180024] [ENSMUST00000180502]

AlphaFold

J3QPC5

Predicted Effect

probably benign
Transcript: ENSMUST00000099111

Predicted Effect

probably null
Transcript: ENSMUST00000180024
AA Change: C142*

SMART Domains

Protein: ENSMUSP00000137192
Gene: ENSMUSG00000096916
AA Change: C142*

Domain	Start	End	E-Value	Type
KRAB	14	75	1.56e-34	SMART
ZnF_C2H2	172	194	7.18e1	SMART
ZnF_C2H2	200	222	3.63e-3	SMART
ZnF_C2H2	228	250	8.94e-3	SMART
ZnF_C2H2	256	278	7.49e-5	SMART
ZnF_C2H2	313	335	1.01e-1	SMART
ZnF_C2H2	341	363	4.4e-2	SMART
ZnF_C2H2	369	391	7.37e-4	SMART
ZnF_C2H2	397	419	8.47e-4	SMART
ZnF_C2H2	425	447	1.92e-2	SMART
ZnF_C2H2	453	475	2.99e-4	SMART
ZnF_C2H2	481	503	7.78e-3	SMART
ZnF_C2H2	509	531	1.95e-3	SMART
ZnF_C2H2	537	559	1.92e-2	SMART
ZnF_C2H2	565	587	2.99e-4	SMART
ZnF_C2H2	593	615	1.79e-2	SMART
ZnF_C2H2	621	643	7.37e-4	SMART
ZnF_C2H2	649	671	4.4e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000180502
AA Change: C142*

SMART Domains

Protein: ENSMUSP00000141063
Gene: ENSMUSG00000096916
AA Change: C142*

Domain	Start	End	E-Value	Type
KRAB	14	75	6.5e-37	SMART
ZnF_C2H2	172	194	3e-1	SMART
ZnF_C2H2	200	222	1.5e-5	SMART
ZnF_C2H2	228	250	3.8e-5	SMART
ZnF_C2H2	256	274	2.5e-1	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad12	T	C	5: 121,738,083 (GRCm39)	T435A	probably benign	Het
Adcy3	T	A	12: 4,184,438 (GRCm39)	I38N	probably damaging	Het
Aff1	A	G	5: 103,902,523 (GRCm39)		probably benign	Het
Asxl1	A	G	2: 153,243,041 (GRCm39)	E1197G	probably damaging	Het
Brinp3	T	A	1: 146,777,105 (GRCm39)	N517K	probably damaging	Het
Carmil1	C	T	13: 24,338,966 (GRCm39)		probably null	Het
Ccdc185	G	A	1: 182,576,564 (GRCm39)	Q42*	probably null	Het
Cdc23	C	A	18: 34,784,742 (GRCm39)	V7L	unknown	Het
Cdh10	A	G	15: 19,013,446 (GRCm39)	E682G	probably damaging	Het
Col6a3	C	A	1: 90,738,330 (GRCm39)	R456L	probably damaging	Het
Egf	C	T	3: 129,529,489 (GRCm39)	R307H	probably benign	Het
Flt4	T	C	11: 49,524,084 (GRCm39)		probably null	Het
Fstl5	A	C	3: 76,536,267 (GRCm39)	K26Q	possibly damaging	Het
Gk5	G	T	9: 96,056,821 (GRCm39)	A413S	probably damaging	Het
Glcci1	T	A	6: 8,537,854 (GRCm39)	S157R	probably benign	Het
Gzmc	G	A	14: 56,471,433 (GRCm39)	A36V	probably damaging	Het
Hexb	T	C	13: 97,318,512 (GRCm39)	N283S	probably benign	Het
Hydin	A	G	8: 111,249,774 (GRCm39)	E2239G	possibly damaging	Het
Igkv10-95	A	T	6: 68,657,544 (GRCm39)	Y20F	probably benign	Het
Il17re	A	G	6: 113,439,923 (GRCm39)	T181A	probably benign	Het
Kcnh6	G	A	11: 105,911,145 (GRCm39)	A514T	possibly damaging	Het
Kcnk10	G	A	12: 98,401,254 (GRCm39)	R460W	probably damaging	Het
Krtap5-2	A	T	7: 141,728,721 (GRCm39)	S320T	unknown	Het
Larp1b	G	T	3: 40,918,487 (GRCm39)	E24*	probably null	Het
Mettl22	A	G	16: 8,296,115 (GRCm39)	T135A	probably benign	Het
Mlh1	A	G	9: 111,070,581 (GRCm39)	V378A	probably benign	Het
Mmrn1	A	T	6: 60,953,474 (GRCm39)	H585L	probably benign	Het
Myh14	A	G	7: 44,278,279 (GRCm39)	F1024L	probably damaging	Het
Nbea	A	T	3: 55,926,874 (GRCm39)	H776Q	probably damaging	Het
Nod2	A	G	8: 89,390,875 (GRCm39)	D372G	possibly damaging	Het
Nptn	C	T	9: 58,526,263 (GRCm39)	R137*	probably null	Het
Or9k2	T	C	10: 129,998,430 (GRCm39)	Y255C	possibly damaging	Het
Pm20d2	C	T	4: 33,181,803 (GRCm39)	V267I	probably benign	Het
Rassf10	A	G	7: 112,553,627 (GRCm39)	D76G	probably benign	Het
Rbbp5	A	G	1: 132,418,303 (GRCm39)	T41A	possibly damaging	Het
Rttn	T	C	18: 89,031,218 (GRCm39)	V643A	possibly damaging	Het
Sbno2	C	A	10: 79,902,762 (GRCm39)	E421*	probably null	Het
Slc17a3	T	C	13: 24,026,525 (GRCm39)		probably null	Het
Slc1a6	T	A	10: 78,632,103 (GRCm39)		probably null	Het
Slco2b1	A	G	7: 99,338,220 (GRCm39)	S106P	possibly damaging	Het
Spef1	T	C	2: 131,016,511 (GRCm39)	N28S	probably damaging	Het
Srebf2	C	A	15: 82,069,603 (GRCm39)	T675N	probably damaging	Het
Srp68	C	A	11: 116,156,300 (GRCm39)	E147D	probably damaging	Het
Tbcd	T	A	11: 121,500,216 (GRCm39)	L1114H	possibly damaging	Het
Tex15	T	C	8: 34,066,420 (GRCm39)	V1950A	probably benign	Het
Tnfsf9	T	A	17: 57,413,263 (GRCm39)	F148Y	possibly damaging	Het
Traf6	A	G	2: 101,520,402 (GRCm39)	D150G	probably benign	Het
Ttn	A	G	2: 76,693,717 (GRCm39)	Y262H	possibly damaging	Het
Unc93b1	A	G	19: 3,994,027 (GRCm39)	Y386C	probably damaging	Het
Vmn1r226	A	T	17: 20,908,125 (GRCm39)	E119V	probably benign	Het
Wrn	A	T	8: 33,842,100 (GRCm39)		probably null	Het
Zfp398	A	G	6: 47,842,838 (GRCm39)	I165V	probably benign	Het

Other mutations in Zfp850

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02278:Zfp850	APN	7	27,707,822 (GRCm39)	missense	probably damaging	0.96
R0610:Zfp850	UTSW	7	27,688,819 (GRCm39)	missense	probably damaging	0.99
R0690:Zfp850	UTSW	7	27,684,642 (GRCm39)	missense	possibly damaging	0.67
R0711:Zfp850	UTSW	7	27,689,698 (GRCm39)	missense	probably benign	0.00
R1310:Zfp850	UTSW	7	27,688,884 (GRCm39)	missense	probably benign	0.40
R1594:Zfp850	UTSW	7	27,688,816 (GRCm39)	missense	probably benign	0.00
R1771:Zfp850	UTSW	7	27,684,700 (GRCm39)	nonsense	probably null
R2189:Zfp850	UTSW	7	27,688,480 (GRCm39)	missense	probably benign	0.02
R2192:Zfp850	UTSW	7	27,684,620 (GRCm39)	missense	probably damaging	1.00
R2417:Zfp850	UTSW	7	27,688,608 (GRCm39)	missense	possibly damaging	0.54
R4321:Zfp850	UTSW	7	27,688,825 (GRCm39)	missense	probably damaging	0.99
R4770:Zfp850	UTSW	7	27,684,411 (GRCm39)	splice site	probably null
R4970:Zfp850	UTSW	7	27,689,658 (GRCm39)	nonsense	probably null
R5112:Zfp850	UTSW	7	27,689,658 (GRCm39)	nonsense	probably null
R5303:Zfp850	UTSW	7	27,707,838 (GRCm39)	missense	probably damaging	1.00
R5315:Zfp850	UTSW	7	27,689,743 (GRCm39)	missense	probably benign	0.02
R5496:Zfp850	UTSW	7	27,706,771 (GRCm39)	missense	probably damaging	0.98
R5547:Zfp850	UTSW	7	27,688,844 (GRCm39)	missense	probably damaging	1.00
R5677:Zfp850	UTSW	7	27,688,513 (GRCm39)	missense	probably damaging	1.00
R5927:Zfp850	UTSW	7	27,689,620 (GRCm39)	missense	probably benign	0.17
R6654:Zfp850	UTSW	7	27,684,640 (GRCm39)	nonsense	probably null
R6950:Zfp850	UTSW	7	27,689,939 (GRCm39)	missense	possibly damaging	0.86
R6987:Zfp850	UTSW	7	27,689,426 (GRCm39)	missense	probably damaging	1.00
R6990:Zfp850	UTSW	7	27,689,801 (GRCm39)	missense	probably benign	0.09
R7640:Zfp850	UTSW	7	27,688,634 (GRCm39)	missense	probably benign	0.05
R7856:Zfp850	UTSW	7	27,689,899 (GRCm39)	missense	probably benign	0.02
R9498:Zfp850	UTSW	7	27,689,275 (GRCm39)	missense	possibly damaging	0.88
R9506:Zfp850	UTSW	7	27,688,987 (GRCm39)	missense	possibly damaging	0.82
Z1186:Zfp850	UTSW	7	27,689,704 (GRCm39)	missense	probably benign	0.00
Z1186:Zfp850	UTSW	7	27,688,549 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGCTAGAACAGTGAAGGCAC -3'
(R):5'- TCTCGGAAAATGCTTTAGAGTCAG -3'

Sequencing Primer
(F):5'- CTGCCACATTCTTTACACTGAAAGGG -3'
(R):5'- AATGCTTTAGAGTCAGGCTATTTTG -3'

Posted On

2016-07-06