Incidental Mutation 'R5168:Zfp746'
ID397393
Institutional Source Beutler Lab
Gene Symbol Zfp746
Ensembl Gene ENSMUSG00000057691
Gene Namezinc finger protein 746
Synonyms2810407L07Rik
MMRRC Submission 042748-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.214) question?
Stock #R5168 (G1)
Quality Score178
Status Validated
Chromosome6
Chromosomal Location48062395-48086593 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to C at 48064395 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Glutamic Acid at position 465 (Q465E)
Ref Sequence ENSEMBL: ENSMUSP00000145506 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000073124] [ENSMUST00000203609]
Predicted Effect possibly damaging
Transcript: ENSMUST00000073124
AA Change: Q466E

PolyPhen 2 Score 0.877 (Sensitivity: 0.83; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000072868
Gene: ENSMUSG00000057691
AA Change: Q466E

DomainStartEndE-ValueType
Pfam:DUF3669 11 81 3.4e-12 PFAM
KRAB 107 156 1.37e-12 SMART
low complexity region 168 183 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
ZnF_C2H2 454 474 3.65e1 SMART
low complexity region 481 511 N/A INTRINSIC
ZnF_C2H2 517 539 1.92e-2 SMART
ZnF_C2H2 545 567 1.82e-3 SMART
ZnF_C2H2 573 595 1.72e-4 SMART
low complexity region 601 623 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000203033
Predicted Effect possibly damaging
Transcript: ENSMUST00000203609
AA Change: Q465E

PolyPhen 2 Score 0.877 (Sensitivity: 0.83; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000145506
Gene: ENSMUSG00000057691
AA Change: Q465E

DomainStartEndE-ValueType
Pfam:DUF3669 11 81 4.6e-9 PFAM
KRAB 107 156 5.7e-15 SMART
low complexity region 168 183 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
ZnF_C2H2 453 473 1.6e-1 SMART
low complexity region 480 510 N/A INTRINSIC
ZnF_C2H2 516 538 8e-5 SMART
ZnF_C2H2 544 566 7.8e-6 SMART
ZnF_C2H2 572 594 7.3e-7 SMART
low complexity region 600 622 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204771
Meta Mutation Damage Score 0.174 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 95.5%
Validation Efficiency 96% (47/49)
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca1 T C 4: 53,086,070 N478D probably benign Het
Acsl1 T A 8: 46,513,266 probably benign Het
Aox1 T A 1: 58,049,402 C116S probably damaging Het
Bag6 T C 17: 35,144,695 L785P probably damaging Het
Calcr T C 6: 3,708,610 N192S probably benign Het
Cntrl T A 2: 35,157,655 L1414H probably damaging Het
Cntrob T A 11: 69,299,990 I849F possibly damaging Het
Col6a3 C T 1: 90,773,639 W2518* probably null Het
Cxcl15 T A 5: 90,795,283 I48K probably damaging Het
Dab2 T C 15: 6,336,443 probably benign Het
Ddx54 A G 5: 120,617,032 E82G probably benign Het
Dock1 T C 7: 135,118,908 W1249R probably damaging Het
Fam205a1 C T 4: 42,851,488 V223I probably damaging Het
Fras1 A C 5: 96,708,757 M2000L probably benign Het
Gm13103 T C 4: 143,853,198 V451A probably benign Het
Gm1966 T C 7: 106,596,847 noncoding transcript Het
Gpr31b A T 17: 13,051,439 I281N probably damaging Het
Haus5 T C 7: 30,657,711 T432A possibly damaging Het
Hecw2 A G 1: 53,913,300 S925P probably damaging Het
Katnal1 A G 5: 148,921,322 M26T possibly damaging Het
Mccc1 C T 3: 35,990,780 W71* probably null Het
Muc6 G A 7: 141,639,559 probably benign Het
Nrbp1 T A 5: 31,250,137 V397D probably damaging Het
Nt5dc1 T A 10: 34,397,240 E187D probably benign Het
Olfr378 T A 11: 73,425,843 I47F probably benign Het
Olfr564 C T 7: 102,804,321 A281V probably benign Het
Polr1c G T 17: 46,247,709 probably benign Het
Ralgapa1 A G 12: 55,758,032 V493A probably benign Het
Ryr2 T C 13: 11,752,321 T1228A probably benign Het
Slc26a3 G A 12: 31,468,554 V674I possibly damaging Het
Srp68 C A 11: 116,265,474 E147D probably damaging Het
Tacr3 T C 3: 134,829,559 I96T probably damaging Het
Tmem236 T C 2: 14,192,328 probably null Het
Tmem62 T A 2: 120,993,607 N254K probably benign Het
Tmem79 A T 3: 88,333,344 L99Q probably damaging Het
Trav6-5 A T 14: 53,491,516 N78Y probably benign Het
Trim33 C T 3: 103,341,681 Q807* probably null Het
Ugt1a10 A G 1: 88,055,809 T110A probably benign Het
Vcl C T 14: 21,010,102 T603I probably damaging Het
Vps8 A T 16: 21,457,445 T243S probably damaging Het
Vps8 A C 16: 21,533,099 I323L probably benign Het
Other mutations in Zfp746
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01993:Zfp746 APN 6 48082158 missense probably damaging 0.99
R0020:Zfp746 UTSW 6 48064707 missense probably benign 0.05
R0020:Zfp746 UTSW 6 48064707 missense probably benign 0.05
R0734:Zfp746 UTSW 6 48064899 missense probably damaging 0.97
R1109:Zfp746 UTSW 6 48064922 missense possibly damaging 0.94
R1656:Zfp746 UTSW 6 48064477 missense probably damaging 0.99
R1657:Zfp746 UTSW 6 48082174 missense possibly damaging 0.94
R2474:Zfp746 UTSW 6 48064769 missense probably damaging 1.00
R4748:Zfp746 UTSW 6 48064556 missense probably benign 0.05
R5386:Zfp746 UTSW 6 48064176 missense possibly damaging 0.94
R5446:Zfp746 UTSW 6 48064173 missense probably damaging 0.97
R5560:Zfp746 UTSW 6 48082174 missense possibly damaging 0.94
R7251:Zfp746 UTSW 6 48064877 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCTCACCAGTGTGTAGCATGC -3'
(R):5'- ACAACGGAGAGGCCATCTTG -3'

Sequencing Primer
(F):5'- TGTGAAGCAGCGACCAC -3'
(R):5'- ATCTTGGACCCCAGCCAG -3'
Posted On2016-07-06